FastQCFastQC Report
Mon 16 Jan 2017
ERR1146530_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146530_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4466950
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62450.035.1572071
GGTATCA40350.033.556671
CAACGCA65150.031.7372175
ATCAACG67100.030.814553
GTACATG146250.027.9996261
TACATGG144850.027.510482
AACGCAG78200.027.401796
ACATGGG143700.027.1750263
TATCAAC79850.027.0121172
TCAACGC78350.026.9300964
ACGCAGA82500.025.0035847
CATGGGG119300.024.4614224
CGCAGAG84800.024.270558
GTGATCG14350.023.5766438
TGATCGC14600.023.1729349
GTATAAG23550.022.359241
CTAACGC10150.022.2229083
TCTAACG9950.022.1970672
GTACTAG21450.021.4797271
TAACGCC10950.020.5995464
Produced by FastQC (version 0.11.5)