##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146530_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4466950
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16862736318965	31.0	31.0	34.0	28.0	34.0
2	31.38087061641612	31.0	31.0	34.0	28.0	34.0
3	31.48128096352097	31.0	31.0	34.0	28.0	34.0
4	35.15780163198603	37.0	35.0	37.0	32.0	37.0
5	35.167830622684384	37.0	35.0	37.0	32.0	37.0
6	35.392061249846094	37.0	35.0	37.0	33.0	37.0
7	35.37908774443412	37.0	35.0	37.0	33.0	37.0
8	35.282243589026066	37.0	35.0	37.0	33.0	37.0
9	37.003380606454066	39.0	37.0	39.0	33.0	39.0
10-11	36.97150941917863	39.0	37.5	39.0	33.0	39.0
12-13	36.93163534402669	39.0	38.0	39.0	33.0	39.0
14-15	38.35476645138182	40.0	39.0	41.0	34.0	41.0
16-17	38.35626042377909	40.0	38.5	41.0	33.5	41.0
18-19	38.36357044515833	40.0	38.5	41.0	33.5	41.0
20-21	38.45292246387356	40.0	39.0	41.0	34.0	41.0
22-23	38.46364555233437	40.0	39.0	41.0	34.0	41.0
24-25	38.50654283123832	40.5	39.0	41.0	34.0	41.0
26-27	38.534156527384454	40.0	39.0	41.0	34.0	41.0
28-29	38.49623288821232	40.0	39.0	41.0	34.0	41.0
30-31	38.48665274963902	40.0	38.0	41.0	34.0	41.0
32-33	38.501636463358665	40.0	38.0	41.0	34.0	41.0
34-35	38.44832615095311	40.0	38.0	41.0	33.0	41.0
36-37	38.35822720200584	40.0	38.0	41.0	33.0	41.0
38-39	38.318685456519546	40.0	38.0	41.0	33.0	41.0
40-41	38.25505512710015	40.0	38.0	41.0	33.0	41.0
42-43	38.129194304838876	40.0	38.0	41.0	33.0	41.0
44-45	37.98283638724409	40.0	38.0	41.0	33.0	41.0
46-47	37.87131029001892	40.0	37.5	41.0	33.0	41.0
48-49	37.74762522526556	40.0	37.0	41.0	32.0	41.0
50-51	37.619101176417914	40.0	37.0	41.0	32.0	41.0
52-53	37.483219534581764	40.0	36.5	41.0	32.0	41.0
54-55	37.29011350026305	40.0	36.0	41.0	31.0	41.0
56-57	37.143771924915214	39.0	35.5	41.0	31.0	41.0
58-59	36.90650656488208	39.0	35.0	41.0	31.0	41.0
60-61	36.7080168795263	39.0	35.0	41.0	31.0	41.0
62-63	36.456473768454984	38.0	35.0	40.0	31.0	41.0
64-65	36.190222411264955	37.5	35.0	40.0	31.0	41.0
66-67	35.91242704753803	37.0	35.0	40.0	30.0	41.0
68-69	35.608097247562654	36.5	35.0	39.5	30.0	41.0
70-71	35.33839946719797	36.0	35.0	39.0	30.0	41.0
72-73	35.01669125465922	35.5	34.0	39.0	30.0	40.5
74-75	34.703167373711366	35.0	34.0	37.5	29.5	40.0
76-77	34.40616584022655	35.0	34.0	37.0	29.0	39.0
78-79	34.136168862422906	35.0	34.0	37.0	29.0	39.0
80-81	33.87600006715992	35.0	34.0	36.0	29.0	38.5
82-83	33.64059190275244	35.0	34.0	36.0	29.0	37.0
84-85	33.433149800199246	35.0	34.0	35.5	29.0	37.0
86-87	33.25372368170676	35.0	34.0	35.0	29.0	36.5
88-89	33.124021424014146	35.0	34.0	35.0	28.5	36.0
90-91	32.980377102944956	35.0	34.0	35.0	28.0	36.0
92-93	32.90154098434054	35.0	34.0	35.0	28.0	36.0
94-95	32.81243174873235	35.0	33.0	35.0	28.0	35.5
96-97	32.74266602491633	35.0	33.0	35.0	27.0	35.0
98-99	32.67823917885806	35.0	33.0	35.0	27.0	35.0
100	31.755010689620434	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16624525885084296
1101	2	0.15335401364033174
1101	3	0.1732213553901083
1101	4	0.2633314701566789
1101	5	0.18438098948850978
1101	6	0.25177903598949314
1101	7	0.251161205555853
1101	8	0.3702387008908232
1101	9	0.46870676236378017
1101	10-11	0.42996436893094625
1101	12-13	0.4091109726955864
1101	14-15	0.5297522989300489
1101	16-17	0.5484420485898838
1101	18-19	0.4399629068910187
1101	20-21	0.4605385659145824
1101	22-23	0.4354304556286337
1101	24-25	0.41868888783805147
1101	26-27	0.3799849267512343
1101	28-29	0.4057960666348066
1101	30-31	0.41580235306609836
1101	32-33	0.4510917151079994
1101	34-35	0.47769046548534533
1101	36-37	0.46830114828336633
1101	38-39	0.4305817598253512
1101	40-41	0.4107165994778299
1101	42-43	0.3887200869974521
1101	44-45	0.3253979921092949
1101	46-47	0.3053236234882917
1101	48-49	0.3480921930849874
1101	50-51	0.36287301394823857
1101	52-53	0.29154384487052454
1101	54-55	0.37224586642946633
1101	56-57	0.3229158147670361
1101	58-59	0.3525724578687317
1101	60-61	0.3658042140553164
1101	62-63	0.36290098629888234
1101	64-65	0.3557384673994619
1101	66-67	0.29563578848044614
1101	68-69	0.27344075581338245
1101	70-71	0.31262003016979634
1101	72-73	0.2991797191913008
1101	74-75	0.25842585213728597
1101	76-77	0.27886272160257874
1101	78-79	0.31744532035754247
1101	80-81	0.3120755144973799
1101	82-83	0.289969344402202
1101	84-85	0.2703959316588893
1101	86-87	0.3257736809863587
1101	88-89	0.26195874505106787
1101	90-91	0.24048773543515267
1101	92-93	0.299762887640199
1101	94-95	0.2727515667718805
1101	96-97	0.24855631546370915
1101	98-99	0.2442137195415981
1101	100	0.21609934479431203
1102	1	-0.019575158109599755
1102	2	0.02822348474956371
1102	3	0.040355389922346774
1102	4	0.07236933586264627
1102	5	0.05123778436387738
1102	6	0.08914998259153606
1102	7	0.14856264351848125
1102	8	0.1079136431450678
1102	9	0.18794183982082302
1102	10-11	0.24502592301942627
1102	12-13	0.2458591524699898
1102	14-15	0.25843703257226025
1102	16-17	0.2704219104519794
1102	18-19	0.30017234919768754
1102	20-21	0.25228522988858515
1102	22-23	0.23518666243700181
1102	24-25	0.25006570004627093
1102	26-27	0.28906480943204826
1102	28-29	0.30149590685202554
1102	30-31	0.2898297782261068
1102	32-33	0.2786383215569046
1102	34-35	0.2824134194225536
1102	36-37	0.26165895331966027
1102	38-39	0.22437744640820512
1102	40-41	0.24056775505791705
1102	42-43	0.19559445068198755
1102	44-45	0.18755818890915066
1102	46-47	0.1995815262160363
1102	48-49	0.22345993355735772
1102	50-51	0.16400313552649948
1102	52-53	0.2104887577655532
1102	54-55	0.26841893823545604
1102	56-57	0.20543845968337848
1102	58-59	0.22024061197337375
1102	60-61	0.18717721440214063
1102	62-63	0.14437757300825638
1102	64-65	0.16584583737868286
1102	66-67	0.15588896598290347
1102	68-69	0.20355148632678777
1102	70-71	0.18265628404328993
1102	72-73	0.15470033072261913
1102	74-75	0.12618533086200046
1102	76-77	0.1733653678393523
1102	78-79	0.1712139938003574
1102	80-81	0.19379115457999063
1102	82-83	0.12197446940808021
1102	84-85	0.15775954640033518
1102	86-87	0.15541136820918666
1102	88-89	0.11737673407115778
1102	90-91	0.10555843375426832
1102	92-93	0.13033112095953925
1102	94-95	0.052495927859077085
1102	96-97	0.08685857312364931
1102	98-99	0.07431845981896856
1102	100	-0.03144813017997805
1103	1	0.04278870100514354
1103	2	-0.003938067859895256
1103	3	0.05387968514346042
1103	4	0.0021632361377612597
1103	5	0.04615975398220229
1103	6	0.07833470756779093
1103	7	0.08683171769695264
1103	8	0.10353194888995176
1103	9	0.16819793470563127
1103	10-11	0.2186900943082648
1103	12-13	0.17460405889003283
1103	14-15	0.25597286338590663
1103	16-17	0.2056950638745647
1103	18-19	0.20600030697818994
1103	20-21	0.13142059482828472
1103	22-23	0.18266184991885837
1103	24-25	0.19236928595283587
1103	26-27	0.1419902752950435
1103	28-29	0.19065724176748233
1103	30-31	0.16250615746221797
1103	32-33	0.19929088016237273
1103	34-35	0.22852352966683043
1103	36-37	0.16789410183559994
1103	38-39	0.1891117830612572
1103	40-41	0.22740772499249573
1103	42-43	0.21366568173942824
1103	44-45	0.1642540340196348
1103	46-47	0.11629396556723748
1103	48-49	0.07238165832468013
1103	50-51	0.11782566935338679
1103	52-53	0.09151410751046996
1103	54-55	0.10056738321252112
1103	56-57	0.030967714561015214
1103	58-59	0.12524405916023795
1103	60-61	0.11747778982392276
1103	62-63	0.1174719948291667
1103	64-65	0.139224093645268
1103	66-67	0.06967562190941834
1103	68-69	0.045778791726078794
1103	70-71	0.03110287082458285
1103	72-73	0.11773949023976371
1103	74-75	0.05340178003176277
1103	76-77	0.07038788756213421
1103	78-79	0.09585299186476703
1103	80-81	0.04675149747652796
1103	82-83	0.12751712538889848
1103	84-85	0.13093045678618154
1103	86-87	0.10631797397409315
1103	88-89	0.07434252632624094
1103	90-91	0.11804337147494692
1103	92-93	0.11255483900680474
1103	94-95	0.11123367052547906
1103	96-97	0.09110875303877464
1103	98-99	0.014997991457093462
1103	100	0.07552734223135715
1104	1	0.04111012343266296
1104	2	0.04236384687101946
1104	3	0.0348189964631338
1104	4	0.11472084835642704
1104	5	0.15971188180103013
1104	6	0.130023577410185
1104	7	0.14069868784336848
1104	8	0.18278145279095526
1104	9	0.32145831407005687
1104	10-11	0.32867269015357437
1104	12-13	0.297061304621721
1104	14-15	0.35561275508636925
1104	16-17	0.26330534681085993
1104	18-19	0.22106158759572025
1104	20-21	0.2720262885920661
1104	22-23	0.2696383781708249
1104	24-25	0.23296423182677017
1104	26-27	0.21017331093737823
1104	28-29	0.23843878881258718
1104	30-31	0.2108381355237725
1104	32-33	0.19134419696485594
1104	34-35	0.2252405641952393
1104	36-37	0.18748055748228865
1104	38-39	0.19442062437394725
1104	40-41	0.20729774097447518
1104	42-43	0.2112791419004978
1104	44-45	0.15218936306308706
1104	46-47	0.10154034691785796
1104	48-49	0.15752604138420878
1104	50-51	0.13837992230998708
1104	52-53	0.10299396526434634
1104	54-55	0.13224284466070912
1104	56-57	0.11678026556806742
1104	58-59	0.12354979825425261
1104	60-61	0.11092108979323001
1104	62-63	0.15035143853250332
1104	64-65	0.09546027163769821
1104	66-67	0.04568184667846964
1104	68-69	0.021420797081646015
1104	70-71	0.04902069089442307
1104	72-73	0.07137507920253228
1104	74-75	0.06571087923060759
1104	76-77	0.005481509820654651
1104	78-79	0.08941093423668889
1104	80-81	0.08939450761889844
1104	82-83	0.013097686841184952
1104	84-85	0.061397627342152816
1104	86-87	0.0771723882886306
1104	88-89	0.07792518700127715
1104	90-91	0.08851916035847296
1104	92-93	0.07195653524031087
1104	94-95	0.03258409692779907
1104	96-97	0.08318802437555206
1104	98-99	0.039962671694596
1104	100	-0.05088453966226325
1105	1	-0.03744279947936846
1105	2	0.012013948161072818
1105	3	-0.003676518137908147
1105	4	0.09367869418598929
1105	5	0.0584187336193267
1105	6	0.10669118531271948
1105	7	0.11694210267873473
1105	8	0.16718384648573448
1105	9	0.21600671005801786
1105	10-11	0.1972036865576925
1105	12-13	0.1698214057456724
1105	14-15	0.26239511092272494
1105	16-17	0.21153642075765333
1105	18-19	0.14079500761766184
1105	20-21	0.18597790190822394
1105	22-23	0.1765963383973812
1105	24-25	0.1663210232057324
1105	26-27	0.2082651165279117
1105	28-29	0.17104629233159585
1105	30-31	0.16799468569490728
1105	32-33	0.17455086725909297
1105	34-35	0.1374095950041223
1105	36-37	0.15534998704464442
1105	38-39	0.11540699127384357
1105	40-41	0.07781742181683882
1105	42-43	0.08162240658356268
1105	44-45	0.12775285258620528
1105	46-47	0.07827688026164026
1105	48-49	0.04999860639848208
1105	50-51	0.05861271770683629
1105	52-53	0.04196290360648902
1105	54-55	0.07879044686336556
1105	56-57	0.04019725649486361
1105	58-59	0.06390846032389419
1105	60-61	0.05010768216511963
1105	62-63	0.06334864866942524
1105	64-65	0.04909542988209381
1105	66-67	0.03561220063603088
1105	68-69	0.020375513229090814
1105	70-71	-0.03756867120867469
1105	72-73	-0.014194775839236229
1105	74-75	0.016780584844411806
1105	76-77	0.018009442483283067
1105	78-79	-0.02145746762598577
1105	80-81	0.014791365922597777
1105	82-83	0.015590105229968287
1105	84-85	0.027642082490849873
1105	86-87	0.053610490332502536
1105	88-89	-0.005589106316882919
1105	90-91	0.02001748755212418
1105	92-93	0.06326073971720803
1105	94-95	0.003873821941631661
1105	96-97	-0.003467465687577942
1105	98-99	-0.051709901112666046
1105	100	0.008800771175991429
1106	1	-0.0799104088221867
1106	2	-0.0965998412766993
1106	3	-0.019921558635068948
1106	4	-0.06099095239796526
1106	5	0.013650159503512782
1106	6	-0.019548237870935736
1106	7	0.02917950286233406
1106	8	0.028200098335091184
1106	9	0.058199615268790694
1106	10-11	0.09911863184822067
1106	12-13	0.08157131278308327
1106	14-15	0.07908448978882632
1106	16-17	0.060460608123229065
1106	18-19	0.06651449964757461
1106	20-21	0.08212308944330715
1106	22-23	0.0814582754762796
1106	24-25	0.07931546606916129
1106	26-27	0.09373043171482465
1106	28-29	0.08618036103831628
1106	30-31	0.1094445495535794
1106	32-33	0.10404893164746198
1106	34-35	0.1428576818449656
1106	36-37	0.09317474172771512
1106	38-39	0.05072504078340501
1106	40-41	0.09788150426356168
1106	42-43	0.0383136027452764
1106	44-45	0.017786052994836155
1106	46-47	0.01062944136580768
1106	48-49	0.004698796974778929
1106	50-51	-0.03057731027903543
1106	52-53	0.017247827029535756
1106	54-55	0.05213738513981525
1106	56-57	0.027089400479333392
1106	58-59	0.030171026455590777
1106	60-61	-0.00303261905042973
1106	62-63	0.0034452026885460896
1106	64-65	-0.018181021778680417
1106	66-67	-0.005017195007688713
1106	68-69	-0.016038176137548987
1106	70-71	-0.05228754300853211
1106	72-73	-0.04195865351654504
1106	74-75	-0.020082993075021704
1106	76-77	-0.02246373286680381
1106	78-79	-0.034448062521782674
1106	80-81	0.011630937798436491
1106	82-83	-0.026469631530680715
1106	84-85	-0.0297378279533973
1106	86-87	-0.026484080964344514
1106	88-89	-0.006412907039624827
1106	90-91	-0.01524235095790516
1106	92-93	-0.05941462631322025
1106	94-95	-0.09003426989077212
1106	96-97	-0.09254135937290897
1106	98-99	-0.1009738604244248
1106	100	-0.18679319799183958
1107	1	-0.14944307763784437
1107	2	-0.13883359491727276
1107	3	-0.10566656817745823
1107	4	-0.13602387906965419
1107	5	-0.141517058132699
1107	6	-0.08790558631048384
1107	7	-0.040089135097503004
1107	8	-3.8855439858309637E-4
1107	9	0.015256145772987395
1107	10-11	0.07160064767634111
1107	12-13	-0.029210602761338578
1107	14-15	-0.0038050414849379877
1107	16-17	0.026876455005911737
1107	18-19	-0.041490643751252776
1107	20-21	-0.02486060101968235
1107	22-23	0.005256454818294287
1107	24-25	-0.011422242316811548
1107	26-27	0.050096674186406176
1107	28-29	0.01102583609375074
1107	30-31	0.02737027786822921
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2216	18-19	-0.15093317643601978
2216	20-21	-0.18967672746249065
2216	22-23	-0.169159553517801
2216	24-25	-0.21287866608516026
2216	26-27	-0.16053026660583214
2216	28-29	-0.22847682446772666
2216	30-31	-0.23928776255352346
2216	32-33	-0.19310555836316468
2216	34-35	-0.1853595024268131
2216	36-37	-0.149445529369153
2216	38-39	-0.17784282873182633
2216	40-41	-0.17376813744157005
2216	42-43	-0.14695143444325254
2216	44-45	-0.04736700952952333
2216	46-47	-0.03308028356926229
2216	48-49	-0.0436069615269119
2216	50-51	-0.044020699122157225
2216	52-53	0.04666778390907922
2216	54-55	0.018964299703647214
2216	56-57	0.026461914696852773
2216	58-59	0.015914599536031915
2216	60-61	0.01383437211919869
2216	62-63	0.051505656350634865
2216	64-65	0.025838374132980846
2216	66-67	0.03180130003208603
2216	68-69	0.03199508161756626
2216	70-71	0.015789247623402503
2216	72-73	0.06975596847492227
2216	74-75	0.04585875075193968
2216	76-77	0.040382571332713724
2216	78-79	0.05465942011075953
2216	80-81	0.1042552658671454
2216	82-83	0.09616530518317035
2216	84-85	0.07445579958237403
2216	86-87	0.050790604495190905
2216	88-89	0.03789096254195101
2216	90-91	0.0361847169041809
2216	92-93	0.03453699650165731
2216	94-95	0.0356659406608415
2216	96-97	0.06347010908130102
2216	98-99	0.06664482486716139
2216	100	-0.05625310595760169
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	136.0
1	59.0
2	54.0
3	49.0
4	51.0
5	57.0
6	174.0
7	735.0
8	2253.0
9	2879.0
10	2864.0
11	3544.0
12	4859.0
13	5889.0
14	6997.0
15	8033.0
16	8761.0
17	9763.0
18	11039.0
19	12461.0
20	13900.0
21	15452.0
22	17694.0
23	20896.0
24	25507.0
25	32375.0
26	36667.0
27	35530.0
28	38941.0
29	48929.0
30	55806.0
31	63259.0
32	80758.0
33	110745.0
34	167958.0
35	266240.0
36	530929.0
37	1157547.0
38	1302246.0
39	364390.0
40	524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.845958775185906	19.579580279164286	12.947810081552277	24.62665086409753
2	15.517781028643892	20.52684628821391	39.450111071258895	24.505261611883302
3	18.262157046512847	25.684227777849923	29.93022155094167	26.123393624695563
4	12.590246574900824	17.379351632918116	38.67343495921763	31.356966832963433
5	13.77158726725694	37.9797985225705	34.9498679699069	13.298746240265663
6	30.727252739822486	37.58945325347553	18.295626560667234	13.38766744603475
7	26.96721957404968	32.46266017016163	22.852719120482348	17.717401135306343
8	25.903396243311615	34.57845836971366	20.85976224058029	18.65838314639443
9	26.443632693579524	15.403995062031328	20.371180496008833	37.781191748380316
10-11	24.779688588627852	26.10771382106249	28.613348939424604	20.49924865088505
12-13	25.396257166972262	23.928138859597865	27.893833661318162	22.781770312111707
14-15	22.8358704520155	25.14761051422103	25.311844842609982	26.704674191153487
16-17	22.80584880857375	27.937107139310914	26.125415634309196	23.131628417806134
18-19	22.74840129826022	27.780570112387675	27.206228550485793	22.264800038866312
20-21	23.81751588923582	27.069871219306663	26.490720229560377	22.621892661897146
22-23	23.933528467355423	26.775142944868573	26.194151150773216	23.097177437002788
24-25	23.496834690294413	26.95047273960619	26.497540455574008	23.055152114525388
26-27	23.3009157736726	27.228041189106534	26.19563818152714	23.275404855693726
28-29	23.205457410519557	27.386354973699028	26.289685549192736	23.118502066588675
30-31	23.490213721622435	27.057386436844943	26.246981860321466	23.20541798121116
32-33	23.466994412367168	27.31635333577672	25.876682060624596	23.33997019123151
34-35	23.62030880364894	27.198270284018577	26.26689336686968	22.91452754546281
36-37	23.619864842156314	26.896865427564897	26.16325185790816	23.32001787237063
38-39	23.486227480418815	27.045933177947447	26.316188975782033	23.151650365851705
40-41	23.451988112690973	26.712825430198446	26.57174060409428	23.2634458530163
42-43	23.33428314076874	26.70422604595911	26.361937092144945	23.599553721127204
44-45	23.31348454361761	27.02386053455023	26.369693570709675	23.292961351122486
46-47	23.408657797324867	26.92116571904595	26.17525870157673	23.49491778205246
48-49	23.550344104187026	26.783694038841716	26.313142871986795	23.352818984984463
50-51	23.319126913754534	26.83196022277165	26.25298414869926	23.595928714774562
52-53	23.328857972284112	26.891825361715433	26.226130801244146	23.553185864756312
54-55	23.282703368259146	26.80111007745549	26.292921778148443	23.623264776136917
56-57	23.505422808886788	26.82041603142547	26.185109517168172	23.48905164251957
58-59	23.34873958801295	26.710719089437944	26.485268804730538	23.455272517818567
60-61	23.319897372054076	26.89737462951448	26.319221822804987	23.463506175626456
62-63	23.390913891398277	26.776885396199358	26.18975584565422	23.642444866748153
64-65	23.12530148758509	26.76049714267031	26.24067320022269	23.873528169521908
66-67	23.445115751585803	26.694764371641288	26.149957494000336	23.710162382772573
68-69	23.21864228012534	26.655376241800234	26.413189013873133	23.712792464201293
70-71	23.320306905462488	26.52591898576387	26.22694830088148	23.926825807892158
72-73	23.4390235490718	26.612854101637645	26.272486100641522	23.67563624864903
74-75	23.3405920121239	26.865080507060163	26.2938108780299	23.500516602786035
76-77	23.41629020077882	26.629329133756695	26.132040315219072	23.82234035024541
78-79	23.302303160329902	26.732011616299324	26.193870369758653	23.77181485361212
80-81	23.345828562470082	26.83076009999916	26.3084352595023	23.51497607802846
82-83	23.58257506365882	26.49219185659801	26.660358639347308	23.26487444039586
84-85	23.606966720021678	26.720189637034757	26.147986534158125	23.524857108785437
86-87	23.241160102435355	26.97176914176968	26.205832983704557	23.581237772090407
88-89	23.520075565320706	26.44367462348623	26.28642602166524	23.74982378952783
90-91	23.42516220111595	26.515593970050904	26.31260183757763	23.746641991255515
92-93	23.583027152037502	26.470154503935913	26.445195684867645	23.50162265915894
94-95	23.160911746898268	26.4404562519309	26.642686450140012	23.75594555103082
96-97	23.329487856628027	26.399009096457792	26.453975936639324	23.81752711027486
98-99	23.209526455178413	26.675207620304224	26.28599407888424	23.829271845633123
100	23.526197683066023	26.269945895809403	26.174304573831808	24.029551847292762
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1003.0
1	768.5
2	581.0
3	613.0
4	571.5
5	544.5
6	513.0
7	490.5
8	525.5
9	507.5
10	464.5
11	429.5
12	398.5
13	426.0
14	468.5
15	561.5
16	639.0
17	716.5
18	886.5
19	1149.5
20	1563.0
21	2170.0
22	3135.5
23	4744.0
24	7114.0
25	10163.5
26	14599.5
27	20334.0
28	26001.5
29	32404.0
30	41043.5
31	50796.5
32	62428.5
33	71752.5
34	78725.5
35	86044.5
36	95394.0
37	107468.5
38	114103.5
39	116080.0
40	118028.5
41	120833.0
42	125517.5
43	134418.5
44	143797.5
45	157528.0
46	171612.0
47	179652.5
48	185273.5
49	185767.0
50	183957.0
51	187425.5
52	188413.0
53	185644.5
54	181115.5
55	167183.5
56	154036.5
57	139188.5
58	122349.0
59	109123.0
60	90640.0
61	71133.5
62	56588.5
63	43794.0
64	31290.5
65	20956.0
66	15015.0
67	11165.0
68	7805.0
69	5522.5
70	4087.0
71	2875.5
72	2008.0
73	1455.5
74	1138.0
75	844.5
76	598.0
77	402.5
78	242.0
79	149.0
80	110.0
81	91.0
82	65.0
83	47.5
84	42.0
85	31.5
86	23.0
87	21.0
88	15.5
89	12.5
90	8.5
91	10.0
92	11.5
93	7.5
94	5.5
95	7.0
96	6.0
97	6.5
98	7.5
99	8.0
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060376767145367646
2	0.010029214564747759
3	0.011820145737024144
4	0.009782961528559755
5	0.012178331971479422
6	0.011439572862915411
7	0.003089356272176765
8	0.0055966599133637045
9	0.007521910923560819
10-11	0.007118951409798632
12-13	0.006268259102967349
14-15	0.010432174078509944
16-17	0.009704608289772664
18-19	0.00799203035628337
20-21	0.0072868512071995436
22-23	0.0023617904834394832
24-25	0.0037497621419536817
26-27	0.005260860318561883
28-29	0.006480932179675171
30-31	0.009301648776010477
32-33	0.010723200394004859
34-35	0.01235742508870706
36-37	0.018771197349421866
38-39	0.019353249980411692
40-41	0.018860743908035684
42-43	0.025352869407537584
44-45	0.028442225679714345
46-47	0.03089356272176765
48-49	0.03228153438028185
50-51	0.03314342000693986
52-53	0.031083849158822015
54-55	0.03752000805919027
56-57	0.03990418518228322
58-59	0.03725136838334882
60-61	0.037934160892779185
62-63	0.03349041292156841
64-65	0.03563953032830007
66-67	0.03801251413156628
68-69	0.040508624452926495
70-71	0.04021759813743158
72-73	0.03662454247305209
74-75	0.038516213523769015
76-77	0.038907979717704474
78-79	0.03744165482040318
80-81	0.04281444833723234
82-83	0.03895275299701138
84-85	0.035314924053324974
86-87	0.0335687661603555
88-89	0.03155396859154457
90-91	0.033714279318102953
92-93	0.03569549692743371
94-95	0.038986332956491565
96-97	0.0342627519896126
98-99	0.0314868086725842
100	0.02925933802706545
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4466950.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.893598757363456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.40700057024668	36.18292440357361
2	15.323820111734939	16.210639848402487
3	6.435454605731351	10.211830612103421
4	3.4254400515851	7.247354066237786
5	1.9589533797392058	5.18080470261533
6	1.2075822389757989	3.83240222489427
7	0.7819670763511009	2.8952736944588606
8	0.5281029271388815	2.2346611464538513
9	0.39740479078892427	1.8918152593418995
>10	1.4969496798603514	12.38039104636068
>50	0.028616268860387147	1.0044343379352294
>100	0.008632204633060662	0.6734946916965738
>500	0.0	0.0
>1k	7.609435431873854E-5	0.0539739659259328
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5670647757418373E-4
2	0.0	0.0	0.0	0.0	1.5670647757418373E-4
3	0.0	0.0	0.0	0.0	1.5670647757418373E-4
4	0.0	0.0	0.0	0.0	1.5670647757418373E-4
5	0.0	0.0	0.0	0.0	1.5670647757418373E-4
6	0.0	0.0	0.0	0.0	2.686396758414578E-4
7	0.0	0.0	0.0	0.0	2.686396758414578E-4
8	0.0	0.0	0.0	0.0	2.686396758414578E-4
9	0.0	0.0	0.0	0.0	2.686396758414578E-4
10-11	0.0	0.0	0.0	0.0	2.686396758414578E-4
12-13	0.0	0.0	0.0	0.0	8.954655861381927E-4
14-15	0.0	0.0	0.0	0.0	0.0012536518205934699
16-17	0.0	0.0	0.0	1.119331982672741E-5	0.001611838055048747
18-19	0.0	0.0	0.0	5.596659913363704E-5	0.0017573512127962033
20-21	0.0	0.0	0.0	1.0073987844054667E-4	0.002081957487771298
22-23	0.0	0.0	0.0	2.0147975688109335E-4	0.0021715040463851174
24-25	0.0	0.0	0.0	5.260860318561882E-4	0.0021715040463851174
26-27	0.0	0.0	0.0	0.0011193319826727408	0.002406563762746393
28-29	0.0	0.0	0.0	0.003548282385072589	0.0025184969610136674
30-31	0.0	0.0	0.0	0.011596279340489596	0.0025632702403205767
32-33	0.0	0.0	0.0	0.030568956446792556	0.0026863967584145783
34-35	0.0	0.0	0.0	0.06142893920908002	0.002955036434256036
36-37	0.0	0.0	0.0	0.10931396142781988	0.002955036434256036
38-39	0.0	0.0	0.0	0.17537693504516505	0.003145322871310402
40-41	0.0	0.0	0.0	0.24337635299253407	0.003201289470444039
42-43	0.0	0.0	0.0	0.31217049664760066	0.0032796427092311306
44-45	0.0	0.0	0.0	0.3953704429196655	0.0034923157859389517
46-47	0.0	0.0	0.0	0.49623344787830626	0.0037273755023002272
48-49	0.0	0.0	0.0	0.6012491744926628	0.003850502020394229
50-51	0.0	0.0	0.0	0.7086378849102856	0.004051981777275322
52-53	0.0	0.0	0.0	0.8129708190152116	0.004387781372077145
54-55	0.0	0.0	0.0	0.9262136357022129	0.004544487849651328
56-57	0.0	0.0	0.0	1.05588824589485	0.004690001007398785
58-59	0.0	0.0	0.0	1.1965546961573335	0.004813127525492786
60-61	0.0	0.0	0.0	1.3406239156471418	0.004902674084106605
62-63	0.0	0.0	0.0	1.4849393881731383	0.005260860318561883
64-65	0.0	0.0	0.0	1.6357805661581168	0.005339213557348974
66-67	0.0	0.0	0.0	1.804172869631404	0.005372793516829157
68-69	0.0	0.0	0.0	1.9866687560863676	0.005383986836655884
70-71	0.0	0.0	0.0	2.179350563583653	0.0054847267150964305
72-73	0.0	0.0	0.0	2.375032180794502	0.005719786431457706
74-75	0.0	0.0	0.0	2.5730979751284435	0.005910072868512072
76-77	0.0	0.0	0.0	2.7864090710663874	0.005966039467645709
78-79	0.0	0.0	0.0	3.0189390971468226	0.006077972665912984
80-81	0.0	0.0	0.0	3.2669159045881417	0.006145132584873347
82-83	0.0	0.0	0.0	3.517791781864583	0.00648093217967517
84-85	0.0	0.0	0.0	3.7709287097460233	0.0065257054589820795
86-87	0.0	0.0	0.0	4.035583563729166	0.006883891693437357
88	0.0	0.0	0.0	4.249611032136022	0.007230884608065907
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6245	0.0	35.157207	1
GGTATCA	4035	0.0	33.55667	1
CAACGCA	6515	0.0	31.737217	5
ATCAACG	6710	0.0	30.81455	3
GTACATG	14625	0.0	27.999626	1
TACATGG	14485	0.0	27.51048	2
AACGCAG	7820	0.0	27.40179	6
ACATGGG	14370	0.0	27.175026	3
TATCAAC	7985	0.0	27.012117	2
TCAACGC	7835	0.0	26.930096	4
ACGCAGA	8250	0.0	25.003584	7
CATGGGG	11930	0.0	24.461422	4
CGCAGAG	8480	0.0	24.27055	8
GTGATCG	1435	0.0	23.576643	8
TGATCGC	1460	0.0	23.172934	9
GTATAAG	2355	0.0	22.35924	1
CTAACGC	1015	0.0	22.222908	3
TCTAACG	995	0.0	22.197067	2
GTACTAG	2145	0.0	21.479727	1
TAACGCC	1095	0.0	20.599546	4
>>END_MODULE