FastQCFastQC Report
Mon 16 Jan 2017
ERR1146530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4466950
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122590.2744378155117026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104290.23347026494588027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57800.12939477719696885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72650.059.715131
GTATCAA139250.045.144031
ATCAACG174850.035.1180953
CAACGCA176800.034.703785
TATCAAC182700.034.277122
AACGCAG188450.032.9707076
TCAACGC186100.032.944264
ACGCAGA208150.029.5099057
CGCAGAG211750.028.9638148
GTGGTAT21650.025.8629551
GCAGAGT239900.025.2713289
GTACTAG21150.024.6945841
GTACATG141050.023.8852081
ACATGGG140050.022.9628833
TACATGG143600.022.9027442
GAGTACT157800.022.78363612-13
AGAGTAC218750.022.26844610-11
CAGAGTA230900.022.0329510-11
CTAGTAC28450.021.1534443
GTACTTT172150.020.66605214-15
Produced by FastQC (version 0.11.5)