##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146530_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4466950 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.457776335083224 34.0 31.0 34.0 31.0 34.0 2 32.581035382083975 34.0 31.0 34.0 31.0 34.0 3 32.70072107366324 34.0 31.0 34.0 31.0 34.0 4 36.174004186301616 37.0 37.0 37.0 35.0 37.0 5 36.20104254580866 37.0 37.0 37.0 35.0 37.0 6 36.281563259047 37.0 37.0 37.0 35.0 37.0 7 36.26295705123182 37.0 37.0 37.0 35.0 37.0 8 36.2715501628628 37.0 37.0 37.0 35.0 37.0 9 38.081999126921055 39.0 38.0 39.0 37.0 39.0 10-11 38.047710182563044 39.0 38.0 39.0 36.0 39.0 12-13 38.02926168862423 39.0 38.0 39.0 35.0 39.0 14-15 39.49260188719373 41.0 39.0 41.0 37.0 41.0 16-17 39.48492450105777 41.0 39.0 41.0 37.0 41.0 18-19 39.521412708895326 41.0 39.5 41.0 37.0 41.0 20-21 39.55201401403642 41.0 40.0 41.0 37.0 41.0 22-23 39.514945768365436 41.0 39.0 41.0 37.0 41.0 24-25 39.501465317498514 41.0 39.0 41.0 36.0 41.0 26-27 39.43073730397698 41.0 39.0 41.0 36.0 41.0 28-29 39.347307782715276 41.0 39.0 41.0 36.0 41.0 30-31 39.256571262270676 41.0 39.0 41.0 36.0 41.0 32-33 39.1502133446759 41.0 39.0 41.0 35.0 41.0 34-35 39.07729726099464 41.0 39.0 41.0 35.0 41.0 36-37 38.99743135696616 41.0 39.0 41.0 35.0 41.0 38-39 38.897103056895645 40.5 39.0 41.0 35.0 41.0 40-41 38.8077973785245 40.0 38.5 41.0 35.0 41.0 42-43 38.71272255118146 40.0 38.0 41.0 35.0 41.0 44-45 38.568565128331414 40.0 38.0 41.0 35.0 41.0 46-47 38.34762791166232 40.0 38.0 41.0 34.5 41.0 48-49 38.13725953950682 40.0 38.0 41.0 34.0 41.0 50-51 37.98017159359294 40.0 37.5 41.0 34.0 41.0 52-53 37.83678169668342 40.0 37.0 41.0 33.0 41.0 54-55 37.68028475805639 40.0 37.0 41.0 33.0 41.0 56-57 37.4687664961551 40.0 36.0 41.0 33.0 41.0 58-59 37.243331355846834 39.0 35.5 41.0 33.0 41.0 60-61 37.044731528223956 39.0 35.0 41.0 33.0 41.0 62-63 36.77134901890552 39.0 35.0 41.0 32.5 41.0 64-65 36.464838312495104 37.5 35.0 40.5 32.0 41.0 66-67 36.19525268919509 37.0 35.0 40.0 32.0 41.0 68-69 35.87180570635445 37.0 35.0 39.5 31.5 41.0 70-71 35.586129350003915 36.0 35.0 39.0 31.0 41.0 72-73 35.26645104601573 36.0 35.0 39.0 31.0 41.0 74-75 34.95095848397676 35.0 35.0 38.0 31.0 40.0 76-77 34.658444688210075 35.0 35.0 37.0 31.0 39.0 78-79 34.38505232877019 35.0 35.0 37.0 31.0 39.0 80-81 34.142951790371505 35.0 35.0 36.0 31.0 39.0 82-83 33.91033468026282 35.0 34.0 36.0 31.0 37.0 84-85 33.723322848923765 35.0 34.0 36.0 30.5 37.0 86-87 33.55670714917338 35.0 34.0 35.0 30.0 36.5 88-89 33.42177067126339 35.0 34.0 35.0 30.0 36.0 90-91 33.34871254435353 35.0 34.0 35.0 30.0 36.0 92-93 33.2724996921837 35.0 34.0 35.0 30.0 36.0 94-95 33.20948745788513 35.0 34.0 35.0 30.0 36.0 96-97 33.169598271751426 35.0 34.0 35.0 30.0 35.0 98-99 33.120914718096245 35.0 34.0 35.0 30.0 35.0 100 32.57095758851118 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34440991587482017 1101 2 0.3030346323917641 1101 3 0.3105711747841795 1101 4 0.22955404813896507 1101 5 0.22572473515941738 1101 6 0.18605829780153016 1101 7 0.23724961087394547 1101 8 0.22311788361820106 1101 9 0.28501955028936266 1101 10-11 0.2743461608231854 1101 12-13 0.3042589329854195 1101 14-15 0.4014013936849494 1101 16-17 0.3989660078603592 1101 18-19 0.34196703543059925 1101 20-21 0.3605466132182187 1101 22-23 0.4125261367031925 1101 24-25 0.3745162905503605 1101 26-27 0.4120308730581854 1101 28-29 0.45455737797550455 1101 30-31 0.44650739634525394 1101 32-33 0.42327772417425535 1101 34-35 0.4541226089010806 1101 36-37 0.4531177793609942 1101 38-39 0.4912028040863703 1101 40-41 0.45124727821755073 1101 42-43 0.4296855159873729 1101 44-45 0.4658639642556537 1101 46-47 0.4365630356366452 1101 48-49 0.5073924527243818 1101 50-51 0.5304557648028094 1101 52-53 0.5206349725209876 1101 54-55 0.47901270640055316 1101 56-57 0.5023603569627326 1101 58-59 0.45089625365525166 1101 60-61 0.37302222165741483 1101 62-63 0.4403301337594172 1101 64-65 0.47232227927254655 1101 66-67 0.3785756191968872 1101 68-69 0.34784093535136407 1101 70-71 0.34645273955454314 1101 72-73 0.3800397005488634 1101 74-75 0.31446302139845983 1101 76-77 0.32343026073600356 1101 78-79 0.3071116545926813 1101 80-81 0.2866825928274821 1101 82-83 0.3039716016892271 1101 84-85 0.30971732448837486 1101 86-87 0.33912816797254663 1101 88-89 0.31530246806342177 1101 90-91 0.2782852328406449 1101 92-93 0.29483933795630435 1101 94-95 0.3196941998545242 1101 96-97 0.24528136314131643 1101 98-99 0.24023937501777937 1101 100 0.2648018544918074 1102 1 0.20915400510650528 1102 2 0.21188701261616671 1102 3 0.23096320336878762 1102 4 0.13395247015309053 1102 5 0.09201245761165922 1102 6 0.16600321705161747 1102 7 0.16393771293532922 1102 8 0.19965648868876684 1102 9 0.21442606029900446 1102 10-11 0.2237840966231488 1102 12-13 0.22741907729714939 1102 14-15 0.2974089837494489 1102 16-17 0.2886595331448589 1102 18-19 0.30307979709299815 1102 20-21 0.2956950410284023 1102 22-23 0.31195110346561705 1102 24-25 0.2737966796120759 1102 26-27 0.2928281034507876 1102 28-29 0.30921989407940487 1102 30-31 0.29729476520881093 1102 32-33 0.33275378329564376 1102 34-35 0.37311690593746505 1102 36-37 0.35563192496798024 1102 38-39 0.2850232489384865 1102 40-41 0.33133659569664786 1102 42-43 0.31758104368186224 1102 44-45 0.3141681213735339 1102 46-47 0.3231812837785881 1102 48-49 0.32431514313827137 1102 50-51 0.25533430070439067 1102 52-53 0.2999370388229039 1102 54-55 0.3094518966194926 1102 56-57 0.31813223653521305 1102 58-59 0.27943558873435137 1102 60-61 0.2578987115815181 1102 62-63 0.25700900744440247 1102 64-65 0.2946674866894696 1102 66-67 0.2378579283116622 1102 68-69 0.25171435848645274 1102 70-71 0.23132181523758533 1102 72-73 0.2630697061001257 1102 74-75 0.26493986301650096 1102 76-77 0.21701648324020084 1102 78-79 0.19104841888371737 1102 80-81 0.19178554427208638 1102 82-83 0.2042563556205721 1102 84-85 0.23039736103234532 1102 86-87 0.1972216241280762 1102 88-89 0.12935708289301573 1102 90-91 0.12161927405746553 1102 92-93 0.1431868477892948 1102 94-95 0.2065457612171926 1102 96-97 0.14431749415025052 1102 98-99 0.16824525545243318 1102 100 0.15538786904419766 1103 1 0.20846314418144374 1103 2 0.19844692599409797 1103 3 0.22165098066037103 1103 4 0.1312948073457889 1103 5 0.12151985154277156 1103 6 0.12508024700447606 1103 7 0.12055791047183817 1103 8 0.13483503062946767 1103 9 0.18195242120413724 1103 10-11 0.1882629056222811 1103 12-13 0.16762752259835167 1103 14-15 0.21831581071248962 1103 16-17 0.22854937647087326 1103 18-19 0.22751752675031867 1103 20-21 0.2193910998907569 1103 22-23 0.2672566503703493 1103 24-25 0.22103035595583265 1103 26-27 0.21882308991827415 1103 28-29 0.28212093178340325 1103 30-31 0.3058732014124175 1103 32-33 0.25598861118378125 1103 34-35 0.23369089978158541 1103 36-37 0.3149668369114096 1103 38-39 0.30328934241725847 1103 40-41 0.341896890586618 1103 42-43 0.3036411806670216 1103 44-45 0.31849457084553734 1103 46-47 0.3137835384227614 1103 48-49 0.26146278654584876 1103 50-51 0.23666802474809856 1103 52-53 0.23513906937802886 1103 54-55 0.2521970027748921 1103 56-57 0.23165356419712424 1103 58-59 0.2608188633516235 1103 60-61 0.28029261422192064 1103 62-63 0.2819930636576373 1103 64-65 0.27582496399695344 1103 66-67 0.24232180534126257 1103 68-69 0.2723775499332248 1103 70-71 0.1773143181536767 1103 72-73 0.1988843021988913 1103 74-75 0.17742500879483458 1103 76-77 0.1933206871875086 1103 78-79 0.22354549184099426 1103 80-81 0.22792609006645392 1103 82-83 0.20521090224979588 1103 84-85 0.24315633258801483 1103 86-87 0.20797360582250946 1103 88-89 0.19072631690355735 1103 90-91 0.1305377202741198 1103 92-93 0.13051925734663428 1103 94-95 0.1680716845442234 1103 96-97 0.19744014392000508 1103 98-99 0.1627056108324325 1103 100 0.23752684192267992 1104 1 0.20041510530131035 1104 2 0.21463623465379555 1104 3 0.17791207289880617 1104 4 0.11528137324723531 1104 5 0.09609623298504033 1104 6 0.09541525459620459 1104 7 0.09545172469120189 1104 8 0.1087023568441765 1104 9 0.1904315081439023 1104 10-11 0.17372042599006932 1104 12-13 0.19721874671585482 1104 14-15 0.21494708071433877 1104 16-17 0.22838009918100255 1104 18-19 0.2083697303864085 1104 20-21 0.20074779067209647 1104 22-23 0.26320933637363453 1104 24-25 0.23202916597137602 1104 26-27 0.2657522721484469 1104 28-29 0.2929480415591499 1104 30-31 0.3032760097799354 1104 32-33 0.2881168745681322 1104 34-35 0.31657654044273187 1104 36-37 0.3210094370063672 1104 38-39 0.31274421976806366 1104 40-41 0.3312153684979009 1104 42-43 0.3565571693027536 1104 44-45 0.36571603457908 1104 46-47 0.4073951833729552 1104 48-49 0.38124448981854187 1104 50-51 0.4058889941998629 1104 52-53 0.42072254890478433 1104 54-55 0.3799618520078809 1104 56-57 0.41740360836327994 1104 58-59 0.406073737141746 1104 60-61 0.34301917979259144 1104 62-63 0.33026291412265607 1104 64-65 0.35755067005246133 1104 66-67 0.3192351114553915 1104 68-69 0.3445849342207765 1104 70-71 0.28240168604101257 1104 72-73 0.28432241526448365 1104 74-75 0.22358557600109208 1104 76-77 0.2627960900845778 1104 78-79 0.272917889869305 1104 80-81 0.29444706106679774 1104 82-83 0.29476141975503367 1104 84-85 0.34485270304062965 1104 86-87 0.2938555137712697 1104 88-89 0.30494150340592086 1104 90-91 0.2570574629442035 1104 92-93 0.27155169316663574 1104 94-95 0.3437201344198684 1104 96-97 0.2708386319571119 1104 98-99 0.2725291084112129 1104 100 0.3544682289730261 1105 1 0.1607352320671751 1105 2 0.16732103607571958 1105 3 0.15756991347060278 1105 4 0.12343892838852355 1105 5 0.08301001548812081 1105 6 0.08572872610429982 1105 7 0.10134177144036016 1105 8 0.10701694934295602 1105 9 0.15341843379071918 1105 10-11 0.10588836278397906 1105 12-13 0.1253268763586206 1105 14-15 0.19669994747692954 1105 16-17 0.19123963952237233 1105 18-19 0.17462252802373968 1105 20-21 0.17771796365260428 1105 22-23 0.19955622304554765 1105 24-25 0.17277961278782072 1105 26-27 0.1745870425420577 1105 28-29 0.24072494942572575 1105 30-31 0.27056363818151397 1105 32-33 0.24213189079789288 1105 34-35 0.19812065765819398 1105 36-37 0.20376709907638713 1105 38-39 0.2023114506725392 1105 40-41 0.17598540867297885 1105 42-43 0.12783637878451515 1105 44-45 0.14013049615824258 1105 46-47 0.1887146278780989 1105 48-49 0.17094252794746012 1105 50-51 0.18841405319986393 1105 52-53 0.2102188436783763 1105 54-55 0.11062933976859313 1105 56-57 0.19370414676601655 1105 58-59 0.14875151114097918 1105 60-61 0.1428626457319382 1105 62-63 0.18544184489286408 1105 64-65 0.16047091573625494 1105 66-67 0.12050120899281325 1105 68-69 0.13392559412411487 1105 70-71 0.11297410939533137 1105 72-73 0.1019519295905198 1105 74-75 0.0567754176973736 1105 76-77 0.040048621336751467 1105 78-79 0.07139469881244764 1105 80-81 0.0282563200552417 1105 82-83 0.07954837969597861 1105 84-85 0.04424793505860691 1105 86-87 0.10672588810797379 1105 88-89 0.12001616805473958 1105 90-91 0.11442283759500071 1105 92-93 0.06785731948833984 1105 94-95 0.09715010241243505 1105 96-97 0.0940036997092335 1105 98-99 0.08149834523460697 1105 100 0.13814405185399892 1106 1 0.13523214925771754 1106 2 0.146304875330884 1106 3 0.14803781434791574 1106 4 0.10182337176262024 1106 5 0.05950408781983896 1106 6 0.07692730928501135 1106 7 0.07125562731251733 1106 8 0.05968392656418331 1106 9 0.09658987357553883 1106 10-11 0.08567612758947973 1106 12-13 0.11666206838419413 1106 14-15 0.17306257754962218 1106 16-17 0.1482687375899232 1106 18-19 0.11463204125463022 1106 20-21 0.11448902252458737 1106 22-23 0.11725392526943068 1106 24-25 0.15557133368766074 1106 26-27 0.14656011252256462 1106 28-29 0.11942194127403383 1106 30-31 0.1293641679303832 1106 32-33 0.10959634145076791 1106 34-35 0.09301583110803335 1106 36-37 0.14011672757369098 1106 38-39 0.12954838454479045 1106 40-41 0.10271640073177934 1106 42-43 0.13603028422712526 1106 44-45 0.07892783964604888 1106 46-47 0.12844199277787993 1106 48-49 0.15510166790151914 1106 50-51 0.14686828983621325 1106 52-53 0.15337067201443233 1106 54-55 0.13243769036184005 1106 56-57 0.13986622460141263 1106 58-59 0.09058110107709183 1106 60-61 0.03557435527727648 1106 62-63 0.06076264671821008 1106 64-65 0.08080506317647007 1106 66-67 0.05276833562547978 1106 68-69 0.0491651402988893 1106 70-71 0.011051815805238618 1106 72-73 0.04753219711657408 1106 74-75 0.007708807579454913 1106 76-77 0.022502716041827853 1106 78-79 0.05261538203408378 1106 80-81 0.10594038536951444 1106 82-83 0.08126749819731316 1106 84-85 0.07084941483591933 1106 86-87 0.09170158837215325 1106 88-89 0.06675417036735354 1106 90-91 0.06088679508466299 1106 92-93 0.06068594251769355 1106 94-95 0.10681300845993036 1106 96-97 0.06804039517532345 1106 98-99 0.060833704125322186 1106 100 0.10968517847658887 1107 1 0.09666050015689365 1107 2 0.07363459601162248 1107 3 0.11098427977633918 1107 4 0.056502641166716217 1107 5 0.07155162480874822 1107 6 0.06321654942730959 1107 7 0.08575875417425749 1107 8 0.07566304194075002 1107 9 0.08231510081474624 1107 10-11 0.07771815297715534 1107 12-13 0.08262729040909988 1107 14-15 0.14437596239316974 1107 16-17 0.14238434857150395 1107 18-19 0.11031449069255217 1107 20-21 0.09941600524308569 1107 22-23 0.09549835449281119 1107 24-25 0.1093238993837744 1107 26-27 0.10695872021432962 1107 28-29 0.11501667392134607 1107 30-31 0.11099524741521805 1107 32-33 0.09507842931849098 1107 34-35 0.09766876603568875 1107 36-37 0.10268952473418835 1107 38-39 0.14033530855672893 1107 40-41 0.1285223008894647 1107 42-43 0.1340343870539371 1107 44-45 0.09766021710154149 1107 46-47 0.09510914352017608 1107 48-49 0.08891944658900997 1107 50-51 0.15267851236997387 1107 52-53 0.12183306105343661 1107 54-55 0.04200625319467122 1107 56-57 0.1050398000182966 1107 58-59 0.04703046352661744 1107 60-61 0.12129887355531821 1107 62-63 0.1474879322751832 1107 64-65 0.15823174001253193 1107 66-67 0.09941291395939089 1107 68-69 0.09971140630339903 1107 70-71 0.05718363226662149 1107 72-73 0.11517587324024703 1107 74-75 0.05561549685663891 1107 76-77 0.061940041017045644 1107 78-79 0.0701273972104346 1107 80-81 0.08591996271431412 1107 82-83 0.041301299635591704 1107 84-85 0.0726502333429977 1107 86-87 0.09186150485196265 1107 88-89 0.08181260820575176 1107 90-91 0.07200465530268474 1107 92-93 0.02629893663217331 1107 94-95 0.08437011127874428 1107 96-97 0.03449482326664821 1107 98-99 0.08591903674816592 1107 100 0.02822776017006845 1108 1 0.08831767559907178 1108 2 0.08077635918363768 1108 3 0.09395585416893226 1108 4 0.0782154093670897 1108 5 0.03259693033950839 1108 6 0.02220687831926682 1108 7 0.05093077055603601 1108 8 0.02867179105453488 1108 9 0.08434444950881925 1108 10-11 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52-53 -0.3887661782203793 2214 54-55 -0.40587438499869677 2214 56-57 -0.3701253041612915 2214 58-59 -0.40969976243893313 2214 60-61 -0.486798173117009 2214 62-63 -0.3856753653787166 2214 64-65 -0.34497833157888635 2214 66-67 -0.3510958585536983 2214 68-69 -0.3226837901550965 2214 70-71 -0.332178656673527 2214 72-73 -0.3726333655138063 2214 74-75 -0.3381227423474016 2214 76-77 -0.3615823750261029 2214 78-79 -0.3449714021383201 2214 80-81 -0.3280240649576527 2214 82-83 -0.3159879472286278 2214 84-85 -0.2471415245357349 2214 86-87 -0.2630093014026116 2214 88-89 -0.24231006322337834 2214 90-91 -0.31227862740453816 2214 92-93 -0.3678145616921782 2214 94-95 -0.3146157619526093 2214 96-97 -0.3123977418359374 2214 98-99 -0.3385695006168987 2214 100 -0.29916505989707076 2215 1 -0.3559757026308077 2215 2 -0.36412006506148487 2215 3 -0.34699682774391505 2215 4 -0.24030325056846635 2215 5 -0.25762096855282124 2215 6 -0.26113242148496596 2215 7 -0.23628075831116746 2215 8 -0.2571002256273971 2215 9 -0.3697311634326752 2215 10-11 -0.3189595620273238 2215 12-13 -0.34955487195164636 2215 14-15 -0.46375478694565686 2215 16-17 -0.42139041122499776 2215 18-19 -0.45698374593224145 2215 20-21 -0.4650265740901176 2215 22-23 -0.42611894602998746 2215 24-25 -0.437713785055422 2215 26-27 -0.4453572675844626 2215 28-29 -0.4349835395730963 2215 30-31 -0.43300539840549845 2215 32-33 -0.46678936246392766 2215 34-35 -0.43563287226734815 2215 36-37 -0.4725815465009049 2215 38-39 -0.4886858193878254 2215 40-41 -0.45861501429327944 2215 42-43 -0.4849480272302671 2215 44-45 -0.4909597992485786 2215 46-47 -0.4892708997302435 2215 48-49 -0.46285412297593354 2215 50-51 -0.48378611738172594 2215 52-53 -0.5815573553585978 2215 54-55 -0.5405248836365928 2215 56-57 -0.5007055758253429 2215 58-59 -0.4593488485053072 2215 60-61 -0.4315342244893401 2215 62-63 -0.46521213204002265 2215 64-65 -0.4292895538350052 2215 66-67 -0.4227404978153615 2215 68-69 -0.44635818755008927 2215 70-71 -0.4093923437365987 2215 72-73 -0.4127280471405541 2215 74-75 -0.3834249345444718 2215 76-77 -0.3865324150853837 2215 78-79 -0.3457867993699182 2215 80-81 -0.30865362215106273 2215 82-83 -0.3200724837796258 2215 84-85 -0.3338126439750795 2215 86-87 -0.3023072055792966 2215 88-89 -0.3025824241342079 2215 90-91 -0.34246957458280747 2215 92-93 -0.26613788012998896 2215 94-95 -0.2590359702885934 2215 96-97 -0.309003327782456 2215 98-99 -0.3008985704949865 2215 100 -0.28518899611979265 2216 1 -0.4273275519220263 2216 2 -0.3927188229882077 2216 3 -0.3800124849303401 2216 4 -0.3073235830391994 2216 5 -0.3078292720618805 2216 6 -0.33796664871838544 2216 7 -0.332096507308421 2216 8 -0.36198345598741355 2216 9 -0.42095809862217237 2216 10-11 -0.4602885975260165 2216 12-13 -0.49499128810251847 2216 14-15 -0.5715450307145602 2216 16-17 -0.5657000291063312 2216 18-19 -0.5383209523385659 2216 20-21 -0.5663100395453569 2216 22-23 -0.5602540024495255 2216 24-25 -0.5726670656329844 2216 26-27 -0.6035614098475293 2216 28-29 -0.5481402788892353 2216 30-31 -0.588944624708077 2216 32-33 -0.541483608762789 2216 34-35 -0.6038949380004368 2216 36-37 -0.6704281043186171 2216 38-39 -0.6242296424623888 2216 40-41 -0.5819954724558016 2216 42-43 -0.6376754875886022 2216 44-45 -0.5819131810761107 2216 46-47 -0.5928865728539563 2216 48-49 -0.6024641176152699 2216 50-51 -0.5764171856741314 2216 52-53 -0.6373045612633206 2216 54-55 -0.6205038757204449 2216 56-57 -0.5335935662127511 2216 58-59 -0.5697781448999493 2216 60-61 -0.5867465957247404 2216 62-63 -0.5386375714616989 2216 64-65 -0.4953457738410805 2216 66-67 -0.485533120842355 2216 68-69 -0.4572907869780636 2216 70-71 -0.4545948038668044 2216 72-73 -0.4089137072732285 2216 74-75 -0.45687218455861256 2216 76-77 -0.3952746992068654 2216 78-79 -0.38607359369408556 2216 80-81 -0.43115690507388393 2216 82-83 -0.365757247164197 2216 84-85 -0.4046751280879306 2216 86-87 -0.44390726784047274 2216 88-89 -0.42139887415093114 2216 90-91 -0.4013048610466967 2216 92-93 -0.48427285157394806 2216 94-95 -0.4839678256280706 2216 96-97 -0.4906154232134057 2216 98-99 -0.539252818457328 2216 100 -0.6490727067487647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 26.0 8 79.0 9 158.0 10 236.0 11 238.0 12 231.0 13 215.0 14 380.0 15 708.0 16 1390.0 17 2654.0 18 4663.0 19 7735.0 20 11360.0 21 16347.0 22 24228.0 23 29269.0 24 28329.0 25 25140.0 26 22153.0 27 23847.0 28 28780.0 29 35308.0 30 43590.0 31 56141.0 32 73228.0 33 97242.0 34 143766.0 35 233535.0 36 446232.0 37 1123705.0 38 1495379.0 39 489298.0 40 1360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88507984894387 19.422036571201364 13.358209356202499 24.334674223652268 2 15.629312289825759 20.691866328229516 39.195803247894304 24.483018134050415 3 17.94921390099825 26.028653677435358 30.413140476089733 25.60899194547666 4 12.665935369771319 17.59099609353138 38.20991952003045 31.533149016666854 5 13.22242255845166 37.91915429097136 34.940074185866884 13.918348964710091 6 30.37962384620723 37.889513498507874 17.84641767354185 13.88444498174304 7 26.2650413067174 33.08423713814507 22.79211157324608 17.858609981891444 8 26.04443747971211 34.17954085002071 20.649906535779447 19.126115134487737 9 26.49172253998813 14.631124145110197 20.508534906367878 38.36861840853379 10-11 24.706455187544073 25.843674095299924 28.510739990373747 20.939130726782256 12-13 25.794725707697648 23.74967259539507 28.166959558535464 22.28864213837182 14-15 22.941374526550447 24.65374017030416 26.26370930135983 26.14117600178556 16-17 22.151725450251288 27.556520668465062 26.98906412652929 23.302689754754365 18-19 22.04919464063847 26.538588970102644 29.35191797535231 22.060298413906583 20-21 23.063734763093386 25.813843897961696 29.016017640672047 22.10640369827287 22-23 23.07886383636894 25.637099061614393 28.723863183795036 22.560173918221633 24-25 22.759578683441724 25.800333560930838 28.981385509128156 22.45870224649929 26-27 22.626255050985574 26.068357604181823 28.651619113712933 22.653768231119667 28-29 22.44562228044242 26.212522238333147 28.80927227958054 22.532583201643895 30-31 22.766155806880604 25.86883456070047 28.740191985733432 22.624817646685493 32-33 22.81146785237402 26.0984118743563 28.36480895625001 22.725311317019674 34-35 22.874191159482844 26.073879622681385 28.621872766901703 22.430056450934075 36-37 22.956823921997223 25.82861382277445 28.456846532665253 22.757715722563074 38-39 22.89889551376413 25.92730312854376 28.58553385292344 22.588267504768666 40-41 22.88032556078889 25.70473733662243 28.691947416505986 22.72298968608269 42-43 22.928747596499875 25.708132869091322 28.312308047396385 23.050811487012425 44-45 22.94441945214517 26.055067108147956 28.144958121699425 22.85555531800745 46-47 23.1687653635706 26.02282301401732 27.65559678065861 23.152814841753475 48-49 23.41284254972297 26.121484703849372 27.46309009700074 23.002582649426913 50-51 23.134449397274654 26.300188821443687 27.234557782954848 23.33080399832681 52-53 23.09641002347156 26.3987259160193 27.22821700216627 23.27664705834287 54-55 23.0608018552553 26.325990286057703 27.19561322296991 23.41759463571709 56-57 23.341353047487647 26.319710117219756 26.99724798512797 23.341688850164623 58-59 23.028414154758316 26.28637848421574 27.379302918427705 23.30590444259824 60-61 23.082342471925205 26.486308902501477 27.21030701079337 23.22104161477995 62-63 23.13090985124827 26.340446832774184 27.071198722337115 23.45744459364043 64-65 22.879328710430364 26.307473046374252 27.130958420290746 23.682239822904638 66-67 23.0695230827766 26.25449726808559 27.089547084624034 23.58643256451378 68-69 22.941449400602558 26.1734886055999 27.302020304346925 23.583041689450614 70-71 23.030327927389028 26.07986616140642 27.069048079047693 23.820757832156858 72-73 23.173911179453494 26.07710055162333 27.111874235387866 23.637114033535305 74-75 23.081413486591977 26.386475550812893 26.984836631362004 23.54727433123313 76-77 23.13434606769746 26.193794865882285 26.73506783962067 23.93679122679959 78-79 22.975937864940104 26.29801846442326 26.87122173482389 23.85482193581274 80-81 23.001638479256638 26.410678255193087 26.925089999948508 23.662593265601764 82-83 23.24259046005502 26.054132310590866 27.272649936959137 23.43062729239498 84-85 23.294939843134454 26.228946328325176 26.75459623257185 23.72151759596852 86-87 22.86625358088333 26.475539963010554 26.836222348159524 23.821984107946598 88-89 23.077446580642892 26.046355858317888 26.891374381610156 23.984823179429064 90-91 22.99722405668297 26.07702123372771 26.920561009189715 24.0051937003996 92-93 23.19537939757553 26.02606924187645 26.92848587962704 23.850065480920986 94-95 22.72410705291082 26.01240219836801 27.147740628392974 24.11575012032819 96-97 22.918035987083766 25.93315641862789 27.005871586250617 24.142936008037726 98-99 22.813791889932446 26.1161721938974 26.827947474881796 24.242088441288363 100 23.071880880732532 25.733111733862142 26.8159836379356 24.379023747469734 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 295.0 1 189.5 2 317.0 3 533.5 4 349.5 5 262.5 6 558.0 7 1469.0 8 2873.5 9 3498.0 10 2571.5 11 1642.0 12 1678.5 13 1975.5 14 2324.5 15 2750.0 16 3205.5 17 3713.5 18 4340.5 19 5037.0 20 5834.0 21 6608.0 22 7674.0 23 9229.0 24 11463.5 25 14552.0 26 18778.0 27 24097.0 28 29690.5 29 35963.5 30 43931.5 31 52759.5 32 63548.0 33 72040.0 34 78043.0 35 84819.5 36 93544.0 37 105090.0 38 111444.0 39 113198.0 40 115621.5 41 118850.5 42 123298.5 43 132016.5 44 141465.5 45 154458.5 46 168973.5 47 178318.0 48 183539.0 49 183314.5 50 180898.0 51 183292.0 52 184082.5 53 181319.5 54 178119.5 55 164988.5 56 150990.0 57 137247.0 58 121459.5 59 108034.5 60 89331.0 61 69762.5 62 55066.0 63 42522.5 64 30218.5 65 19900.5 66 14120.0 67 10411.0 68 7262.5 69 5015.5 70 3528.5 71 2397.0 72 1618.0 73 1154.0 74 947.0 75 705.0 76 452.5 77 263.0 78 124.5 79 58.0 80 29.0 81 10.5 82 7.0 83 4.0 84 2.5 85 2.0 86 2.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005954846147818982 2 0.08258431368159483 3 4.253461534156415E-4 4 0.0 5 0.037587167978150635 6 0.0035818623445527708 7 2.238663965345482E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.238663965345482E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.148927120294609E-4 24-25 0.0 26-27 0.0 28-29 2.238663965345482E-5 30-31 7.835323878709187E-5 32-33 0.0018021244921031128 34-35 0.0013879716585141987 36-37 7.163724689105542E-4 38-39 7.051791490838268E-4 40-41 0.0016342246947022019 42-43 6.0443927064328E-4 44-45 4.1415283358891414E-4 46-47 2.5744635601473043E-4 48-49 5.708593111630978E-4 50-51 7.051791490838268E-4 52-53 0.0012200718611132875 54-55 0.0016006447352220194 56-57 9.178522257916476E-4 58-59 0.002843103235988762 60-61 0.0019588309696772967 62-63 9.85012144752012E-4 64-65 0.004566874489304783 66-67 0.004712387647052239 68-69 0.003861695340220956 70-71 0.0017237712533160207 72-73 0.005025800602200607 74-75 0.0037049888626467724 76-77 0.003693795542820045 78-79 7.947257076976461E-4 80-81 6.715991896036445E-5 82-83 1.343198379207289E-4 84-85 7.835323878709187E-5 86-87 1.119331982672741E-5 88-89 1.4551315774745632E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 3.1341295514836746E-4 98-99 4.589261128958238E-4 100 7.163724689105542E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4466950.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.031473918782204 #Duplication Level Percentage of deduplicated Percentage of total 1 65.3968556311553 32.06504221249356 2 16.640476363135196 16.3181416559035 3 7.184494166606377 10.567990150488104 4 3.702991410172 7.262525069972918 5 2.051309727754947 5.0289369707880445 6 1.3507385418974571 3.9737220952883443 7 0.8783130992410576 3.0145490072573873 8 0.6058858716415397 2.376598185052062 9 0.43395656654524495 1.9149777067002702 >10 1.7013547547646422 13.105122192156916 >50 0.03601305216953886 1.2038960259077132 >100 0.015878305328249295 1.3494087201314307 >500 0.0010725059356979769 0.393468723737427 >1k 4.125022829599204E-4 0.27405681127965 >5k 8.250045659198407E-5 0.23380787737215894 >10k+ 1.6500091318396814E-4 0.9177565954705089 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12259 0.2744378155117026 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10429 0.23347026494588027 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5780 0.12939477719696885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.8057287410873193E-4 2 0.0 0.0 0.0 0.0 3.8057287410873193E-4 3 0.0 0.0 0.0 0.0 4.0295951376218675E-4 4 0.0 0.0 0.0 0.0 4.0295951376218675E-4 5 0.0 0.0 0.0 0.0 4.253461534156415E-4 6 0.0 0.0 0.0 0.0 5.596659913363704E-4 7 0.0 0.0 0.0 0.0 5.596659913363704E-4 8 0.0 0.0 0.0 0.0 5.820526309898253E-4 9 0.0 0.0 0.0 0.0 5.820526309898253E-4 10-11 0.0 0.0 0.0 0.0 6.492125499501897E-4 12-13 0.0 0.0 0.0 0.0 0.0016006447352220196 14-15 0.0 0.0 0.0 0.0 0.001947637649850569 16-17 0.0 0.0 0.0 1.119331982672741E-5 0.002272243924825664 18-19 0.0 0.0 0.0 5.596659913363704E-5 0.002552076920493849 20-21 0.0 0.0 0.0 1.0073987844054667E-4 0.002910263154949126 22-23 0.0 0.0 0.0 2.0147975688109335E-4 0.0031565161911371294 24-25 0.0 0.0 0.0 5.484726715096431E-4 0.0031565161911371294 26-27 0.0 0.0 0.0 0.001175298581806378 0.0033803825876716773 28-29 0.0 0.0 0.0 0.0036154423040329533 0.0034587358264587696 30-31 0.0 0.0 0.0 0.011741792498237051 0.003537089065245861 32-33 0.0 0.0 0.0 0.030815209482980558 0.003693795542820045 34-35 0.0 0.0 0.0 0.06166399892544129 0.003940048579008048 36-37 0.0 0.0 0.0 0.10981766082002262 0.003940048579008048 38-39 0.0 0.0 0.0 0.17565676804083324 0.004107948376408959 40-41 0.0 0.0 0.0 0.24363379934854879 0.004163914975542596 42-43 0.0 0.0 0.0 0.3124615229630956 0.004208688254849506 44-45 0.0 0.0 0.0 0.39588533563169503 0.004398974691903872 46-47 0.0 0.0 0.0 0.49730800658167207 0.0045556811694780555 48-49 0.0 0.0 0.0 0.6028274325882313 0.0047347742867056945 50-51 0.0 0.0 0.0 0.7106974557584034 0.004958640683240242 52-53 0.0 0.0 0.0 0.8155117026158789 0.005328020237522247 54-55 0.0 0.0 0.0 0.9292022520959491 0.005372793516829157 56-57 0.0 0.0 0.0 1.0591902752437345 0.005462340075442976 58-59 0.0 0.0 0.0 1.2007298044527026 0.005551886634056795 60-61 0.0 0.0 0.0 1.345291530014887 0.005585466593536977 62-63 0.0 0.0 0.0 1.4902338284511802 0.006022006066779346 64-65 1.119331982672741E-5 0.0 0.0 1.6415898991481885 0.006100359305566438 66-67 2.238663965345482E-5 0.0 0.0 1.8105194819731585 0.00613393926504662 68-69 2.238663965345482E-5 0.0 0.0 1.9932952014237904 0.006156325904700075 70-71 2.238663965345482E-5 0.0 0.0 2.1869396344261745 0.006223485823660439 72-73 2.238663965345482E-5 0.0 0.0 2.3835055239033345 0.006458545540021714 74-75 2.238663965345482E-5 0.0 0.0 2.5827466168190822 0.0067047985762097185 76-77 2.238663965345482E-5 0.0 0.0 2.7971210781405658 0.006839118414130447 78-79 2.238663965345482E-5 0.0 0.0 3.0309047560415943 0.006895085013264085 80-81 2.238663965345482E-5 0.0 0.0 3.279978508825933 0.0069958248917046315 82-83 2.238663965345482E-5 0.0 0.0 3.5322199711212345 0.007253271247719361 84-85 2.238663965345482E-5 0.0 0.0 3.786923963778417 0.0072756578873728155 86-87 2.238663965345482E-5 0.0 0.0 4.052787696302846 0.007589070842521183 88 2.238663965345482E-5 0.0 0.0 4.267654663696706 0.007880097158016096 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7265 0.0 59.71513 1 GTATCAA 13925 0.0 45.14403 1 ATCAACG 17485 0.0 35.118095 3 CAACGCA 17680 0.0 34.70378 5 TATCAAC 18270 0.0 34.27712 2 AACGCAG 18845 0.0 32.970707 6 TCAACGC 18610 0.0 32.94426 4 ACGCAGA 20815 0.0 29.509905 7 CGCAGAG 21175 0.0 28.963814 8 GTGGTAT 2165 0.0 25.862955 1 GCAGAGT 23990 0.0 25.271328 9 GTACTAG 2115 0.0 24.694584 1 GTACATG 14105 0.0 23.885208 1 ACATGGG 14005 0.0 22.962883 3 TACATGG 14360 0.0 22.902744 2 GAGTACT 15780 0.0 22.783636 12-13 AGAGTAC 21875 0.0 22.268446 10-11 CAGAGTA 23090 0.0 22.03295 10-11 CTAGTAC 2845 0.0 21.153444 3 GTACTTT 17215 0.0 20.666052 14-15 >>END_MODULE