FastQCFastQC Report
Mon 16 Jan 2017
ERR1146529_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146529_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4729333
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64600.035.807381
CAACGCA61650.034.6814965
GGTATCA42950.032.949081
ATCAACG65650.032.855393
TATCAAC78650.029.037662
AACGCAG77250.029.0130526
ACGCAGA80250.026.5803957
CGCAGAG82100.025.926418
GTACTAG24800.025.782631
TCAACGC85350.025.5469194
GTACATG140800.025.177271
TACATGG136100.025.1360682
ACATGGG143150.023.3403263
GCATCGT24050.023.0544228
CATGGGG106250.022.9984464
CGTTAAC5550.021.1657839
ATGGGGG75950.020.4795175
GCAGAGT101750.020.319159
GTGATCG15600.019.578358
TGATCGC16000.019.3825669
Produced by FastQC (version 0.11.5)