##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146529_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4729333 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.634054950243513 33.0 31.0 34.0 30.0 34.0 2 31.721304885910975 33.0 31.0 34.0 30.0 34.0 3 31.921818573570523 33.0 31.0 34.0 30.0 34.0 4 35.464684766329626 37.0 35.0 37.0 33.0 37.0 5 35.44120576833985 37.0 35.0 37.0 33.0 37.0 6 35.51961196219425 37.0 35.0 37.0 33.0 37.0 7 35.38949530515191 37.0 35.0 37.0 33.0 37.0 8 35.35828392714152 37.0 35.0 37.0 33.0 37.0 9 37.04691338080867 39.0 38.0 39.0 34.0 39.0 10-11 37.0549169407187 39.0 38.0 39.0 34.0 39.0 12-13 37.036165036380396 39.0 38.0 39.0 34.0 39.0 14-15 38.47560596811432 40.5 39.0 41.0 34.0 41.0 16-17 38.47884966019521 40.5 39.0 41.0 34.0 41.0 18-19 38.495909148287936 40.0 39.0 41.0 34.0 41.0 20-21 38.591802057499436 41.0 39.0 41.0 34.0 41.0 22-23 38.59729162653592 40.0 39.0 41.0 34.0 41.0 24-25 38.64362004113477 41.0 39.0 41.0 34.0 41.0 26-27 38.670604501734175 41.0 39.0 41.0 34.0 41.0 28-29 38.63719947400617 40.0 39.0 41.0 34.0 41.0 30-31 38.62673911944877 40.0 39.0 41.0 34.0 41.0 32-33 38.64268766864164 40.0 39.0 41.0 34.0 41.0 34-35 38.58497339899728 40.0 38.5 41.0 34.0 41.0 36-37 38.49870457419682 40.0 38.0 41.0 34.0 41.0 38-39 38.46178129135758 40.0 38.0 41.0 33.5 41.0 40-41 38.402216232183264 40.0 38.0 41.0 33.0 41.0 42-43 38.278322651418286 40.0 38.0 41.0 33.0 41.0 44-45 38.124157888649414 40.0 38.0 41.0 33.0 41.0 46-47 38.018756979049684 40.0 38.0 41.0 33.0 41.0 48-49 37.90620950142441 40.0 37.5 41.0 33.0 41.0 50-51 37.78057148862218 40.0 37.0 41.0 32.5 41.0 52-53 37.66399215280464 40.0 37.0 41.0 32.0 41.0 54-55 37.48792345559089 40.0 36.0 41.0 32.0 41.0 56-57 37.35762918365021 39.5 36.0 41.0 32.0 41.0 58-59 37.132538668772106 39.0 35.5 41.0 31.5 41.0 60-61 36.95901599654751 39.0 35.0 41.0 31.0 41.0 62-63 36.72657645380437 39.0 35.0 41.0 31.0 41.0 64-65 36.4811026206021 38.0 35.0 40.0 31.0 41.0 66-67 36.21957007468072 37.0 35.0 40.0 31.0 41.0 68-69 35.92615650029296 37.0 35.0 40.0 31.0 41.0 70-71 35.66331362160372 36.5 35.0 39.0 30.5 41.0 72-73 35.33846284454911 36.0 35.0 39.0 30.0 41.0 74-75 35.009450592715716 35.5 34.5 38.5 30.0 40.0 76-77 34.692381251225065 35.0 34.0 37.0 30.0 39.5 78-79 34.406493473815445 35.0 34.0 37.0 30.0 39.0 80-81 34.12376576147207 35.0 34.0 36.5 29.5 39.0 82-83 33.86042661829903 35.0 34.0 36.0 29.0 37.5 84-85 33.631062456375986 35.0 34.0 36.0 29.0 37.0 86-87 33.427803878475046 35.0 34.0 35.0 29.0 37.0 88-89 33.28326383445615 35.0 34.0 35.0 29.0 36.0 90-91 33.12083426986427 35.0 34.0 35.0 29.0 36.0 92-93 33.02438261378508 35.0 34.0 35.0 29.0 36.0 94-95 32.927705238772575 35.0 34.0 35.0 28.0 36.0 96-97 32.848056269245575 35.0 34.0 35.0 28.0 35.0 98-99 32.7776435916016 35.0 34.0 35.0 28.0 35.0 100 31.864143210046745 34.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18624727720659706 1101 2 0.17617825999662529 1101 3 0.2617408754612427 1101 4 0.19357265743804675 1101 5 0.22249381247246447 1101 6 0.21210241458865653 1101 7 0.1753909609322335 1101 8 0.182307243323784 1101 9 0.3543240935916643 1101 10-11 0.3289291701564423 1101 12-13 0.3035659361287415 1101 14-15 0.40773740574424266 1101 16-17 0.3955671581935434 1101 18-19 0.36453469168019836 1101 20-21 0.3270983828757821 1101 22-23 0.3078040426458628 1101 24-25 0.31877684913677484 1101 26-27 0.3130476235978037 1101 28-29 0.42737686270454844 1101 30-31 0.4129090210863282 1101 32-33 0.3526438809009349 1101 34-35 0.37784319649231435 1101 36-37 0.3975847685644993 1101 38-39 0.3738842349965097 1101 40-41 0.3685241628474927 1101 42-43 0.4026996085137142 1101 44-45 0.3399960692871957 1101 46-47 0.2802135189412098 1101 48-49 0.34319254245861686 1101 50-51 0.3973822974191137 1101 52-53 0.3488080812452523 1101 54-55 0.3438179647660746 1101 56-57 0.35736895301320715 1101 58-59 0.34897979341619845 1101 60-61 0.3504234011491292 1101 62-63 0.34856603977320333 1101 64-65 0.3076768691792111 1101 66-67 0.3119306870365861 1101 68-69 0.2672236584209102 1101 70-71 0.26238420926685535 1101 72-73 0.303216471471778 1101 74-75 0.2808699271054351 1101 76-77 0.24681314180877933 1101 78-79 0.15816760619021863 1101 80-81 0.23766018145397538 1101 82-83 0.20124316093560424 1101 84-85 0.24137368210833188 1101 86-87 0.22372790792510244 1101 88-89 0.2215680737768153 1101 90-91 0.24782180297831502 1101 92-93 0.25803147952304073 1101 94-95 0.20354976113205225 1101 96-97 0.22150849266096628 1101 98-99 0.20374805049357292 1101 100 0.09797156916337357 1102 1 0.16190726433569935 1102 2 0.1622612830761625 1102 3 0.2455745129943736 1102 4 0.16192927775362165 1102 5 0.15866521529108013 1102 6 0.19281157407785798 1102 7 0.10666998233251235 1102 8 0.16230527604363232 1102 9 0.21807273947930383 1102 10-11 0.2743547873584333 1102 12-13 0.21781396900932037 1102 14-15 0.31093039142616874 1102 16-17 0.27948038699530287 1102 18-19 0.30163600082155995 1102 20-21 0.28738597866014715 1102 22-23 0.2426123389105399 1102 24-25 0.2890989623299518 1102 26-27 0.32406515103526345 1102 28-29 0.35617016778019206 1102 30-31 0.356616971276857 1102 32-33 0.3417228787710016 1102 34-35 0.3389623988823729 1102 36-37 0.3163130441020172 1102 38-39 0.31146639626590655 1102 40-41 0.25547713520849413 1102 42-43 0.2537570601658814 1102 44-45 0.22262665715036434 1102 46-47 0.18511864267733813 1102 48-49 0.24321044085052534 1102 50-51 0.19963334165269941 1102 52-53 0.22768376135520896 1102 54-55 0.23727274657477437 1102 56-57 0.2596257062166316 1102 58-59 0.26144572764603424 1102 60-61 0.18484414322520593 1102 62-63 0.192063272401505 1102 64-65 0.1502853797874124 1102 66-67 0.15638350349384922 1102 68-69 0.15775398185848388 1102 70-71 0.13509060170581222 1102 72-73 0.16711059322584276 1102 74-75 0.13489091271278397 1102 76-77 0.15630409842768245 1102 78-79 0.1550058141137356 1102 80-81 0.14511514456103924 1102 82-83 0.07882959107433862 1102 84-85 0.10829940828222817 1102 86-87 0.15470709268677751 1102 88-89 0.06696869494873425 1102 90-91 0.11699755760061947 1102 92-93 0.14767902135507427 1102 94-95 0.04011019681388461 1102 96-97 0.06960963657650865 1102 98-99 0.07918460345873513 1102 100 -0.039331649782692324 1103 1 0.03988813365123178 1103 2 0.07814495559330226 1103 3 0.13887054380036545 1103 4 0.04688610424431516 1103 5 0.0811176193171903 1103 6 0.12134623850893433 1103 7 0.09125065577772773 1103 8 0.14118768719696106 1103 9 0.20100476940336875 1103 10-11 0.2068793096040764 1103 12-13 0.2255666904273781 1103 14-15 0.27134967952474653 1103 16-17 0.2519800024331573 1103 18-19 0.2072223042564616 1103 20-21 0.1940624388425789 1103 22-23 0.2240592795905485 1103 24-25 0.19744635781878372 1103 26-27 0.19784670435864626 1103 28-29 0.20638880417412508 1103 30-31 0.214123175546014 1103 32-33 0.21069205996130336 1103 34-35 0.20399683001242153 1103 36-37 0.310726368923703 1103 38-39 0.2324362619768081 1103 40-41 0.2095680944090148 1103 42-43 0.19226731771996697 1103 44-45 0.17755161625859017 1103 46-47 0.12713732470598416 1103 48-49 0.12181415982276889 1103 50-51 0.15546705954817241 1103 52-53 0.2025528692223304 1103 54-55 0.13319761851558098 1103 56-57 0.14686908790223896 1103 58-59 0.1829045512960903 1103 60-61 0.1454536229382697 1103 62-63 0.09563513681003144 1103 64-65 0.10774823108492626 1103 66-67 0.1387895934198653 1103 68-69 0.16685710604128445 1103 70-71 0.09220287333999977 1103 72-73 0.16101268114188372 1103 74-75 0.15285692511106674 1103 76-77 0.1728694714273189 1103 78-79 0.11474376065446279 1103 80-81 0.14489671326691678 1103 82-83 0.09669439953675862 1103 84-85 0.12221410983637071 1103 86-87 0.1078059969410674 1103 88-89 0.043657448300841395 1103 90-91 0.10224417729942559 1103 92-93 0.12036818747053246 1103 94-95 -0.0023613327691975883 1103 96-97 0.04110836495770798 1103 98-99 0.015579641068917738 1103 100 -0.0803832477665587 1104 1 0.11557769539342289 1104 2 0.0962899803172732 1104 3 0.1330651519423398 1104 4 0.10420948025189603 1104 5 0.13135910140077556 1104 6 0.15132998165057643 1104 7 0.0730378941439156 1104 8 0.07934388851106178 1104 9 0.19304263433596702 1104 10-11 0.21957930283038252 1104 12-13 0.21271496652302346 1104 14-15 0.26170174211365094 1104 16-17 0.24004594431489323 1104 18-19 0.14320686024102258 1104 20-21 0.1949931439838153 1104 22-23 0.21282562112004655 1104 24-25 0.20439836621816454 1104 26-27 0.21979651130845212 1104 28-29 0.18149413506893097 1104 30-31 0.18358788461598152 1104 32-33 0.21273686792716262 1104 34-35 0.16766952526406698 1104 36-37 0.18132397781079135 1104 38-39 0.1689654227164965 1104 40-41 0.1964455922075672 1104 42-43 0.20766323995799496 1104 44-45 0.16643937619897997 1104 46-47 0.09681980794109535 1104 48-49 0.0995370142561498 1104 50-51 0.12553394974664656 1104 52-53 0.05589991927990923 1104 54-55 0.13597957340279976 1104 56-57 0.09744315821315297 1104 58-59 0.14307050672520916 1104 60-61 0.15843099881038114 1104 62-63 0.11601378613604396 1104 64-65 0.10726408152182643 1104 66-67 0.07294372875821153 1104 68-69 0.09933322878057282 1104 70-71 0.07591587205299533 1104 72-73 0.08516139752744323 1104 74-75 0.058300432528263 1104 76-77 0.03202377610794116 1104 78-79 0.0741197343725446 1104 80-81 0.058081025398593056 1104 82-83 0.08608747682457363 1104 84-85 0.16910820317783504 1104 86-87 0.09226782719236581 1104 88-89 0.05888284286834278 1104 90-91 0.09441091170300808 1104 92-93 0.08551145103484714 1104 94-95 0.06149323608536861 1104 96-97 0.049776487967932326 1104 98-99 0.042591427291220896 1104 100 0.030642289048451232 1105 1 0.07672859200751958 1105 2 0.09255503937956178 1105 3 0.08802982979281637 1105 4 0.11157329241350311 1105 5 0.13519569882175375 1105 6 0.09400983438346344 1105 7 0.06386087468330715 1105 8 0.10934896481320777 1105 9 0.14319132542178892 1105 10-11 0.20151165648450586 1105 12-13 0.16220039036164025 1105 14-15 0.2174739324564996 1105 16-17 0.20695338930440244 1105 18-19 0.1951447358683538 1105 20-21 0.19983733846596863 1105 22-23 0.14402858515874328 1105 24-25 0.18067286046302655 1105 26-27 0.17883201390041137 1105 28-29 0.26415980241787906 1105 30-31 0.22450571700235145 1105 32-33 0.2076942628602083 1105 34-35 0.2239554439062701 1105 36-37 0.20880118608374687 1105 38-39 0.22231082540204739 1105 40-41 0.20875712497905852 1105 42-43 0.2259084373087461 1105 44-45 0.15599936841604745 1105 46-47 0.16633642875437005 1105 48-49 0.19134789536958863 1105 50-51 0.15523681758560315 1105 52-53 0.12109583519372791 1105 54-55 0.1406223897830614 1105 56-57 0.125869471937321 1105 58-59 0.17552029535755764 1105 60-61 0.13777917770057257 1105 62-63 0.14043797368312028 1105 64-65 0.164748170521257 1105 66-67 0.13812224176039223 1105 68-69 0.1301737318018965 1105 70-71 0.09890085728606834 1105 72-73 0.14189457425993623 1105 74-75 0.1926680850700535 1105 76-77 0.17623663947932044 1105 78-79 0.11090579378158338 1105 80-81 0.11821185703167458 1105 82-83 0.09214830983815148 1105 84-85 0.1441224483469199 1105 86-87 0.1311502689480477 1105 88-89 0.11814582249464678 1105 90-91 0.11469765211917604 1105 92-93 0.10954668731116612 1105 94-95 0.06526179801353749 1105 96-97 0.025866333080934112 1105 98-99 -0.001090370089315229 1105 100 0.07644907868409945 1106 1 0.002287872721922213 1106 2 -0.016931161655051596 1106 3 0.0051871450141369735 1106 4 0.029980978406022984 1106 5 0.04324799243727995 1106 6 -0.0191323781907613 1106 7 -0.023942989942909776 1106 8 0.04733584784383993 1106 9 0.003232676708393001 1106 10-11 0.07805456124205534 1106 12-13 0.08206632263394908 1106 14-15 0.057139715796793666 1106 16-17 0.025763816544738916 1106 18-19 0.008840760759241562 1106 20-21 -0.005174348440476706 1106 22-23 0.06610434062661596 1106 24-25 -0.006113173986250331 1106 26-27 0.05179838832318495 1106 28-29 0.06392261415010125 1106 30-31 0.019686657684040654 1106 32-33 -0.0030806321211613863 1106 34-35 0.013050007317524148 1106 36-37 0.027517503613296412 1106 38-39 -0.01587420419998864 1106 40-41 0.012907032870060675 1106 42-43 -0.04945264315166753 1106 44-45 -0.03626897995275158 1106 46-47 -0.08398623458595722 1106 48-49 -0.09426413385835275 1106 50-51 -0.06829533211267602 1106 52-53 -0.07605196127838809 1106 54-55 -0.08563781625815636 1106 56-57 -0.11860456606755321 1106 58-59 -0.08047331426685389 1106 60-61 -0.10996140464899895 1106 62-63 -0.07907032517050538 1106 64-65 -0.09126488871867622 1106 66-67 -0.051046473826225736 1106 68-69 -0.08986367092370529 1106 70-71 -0.06611579236941623 1106 72-73 -0.08153226464857255 1106 74-75 -0.06013565340207805 1106 76-77 0.005838733381160921 1106 78-79 -0.03638742469908607 1106 80-81 -0.04205511185972455 1106 82-83 -0.01608915155013335 1106 84-85 -0.03888609705433055 1106 86-87 -0.07284398833617445 1106 88-89 -0.1036429231319218 1106 90-91 -0.058443847302477536 1106 92-93 -0.07567839160946477 1106 94-95 -0.0876821015732574 1106 96-97 -0.10062947552692236 1106 98-99 -0.09845989424311341 1106 100 -0.11272839129422252 1107 1 0.09421812171826716 1107 2 0.07142068021178716 1107 3 0.1203086316862283 1107 4 0.159995037676687 1107 5 0.13661396356317823 1107 6 0.1374316822757038 1107 7 -0.013198770045264041 1107 8 -0.005198888201093155 1107 9 0.10109922217914402 1107 10-11 0.09768016967124993 1107 12-13 0.1084714678294958 1107 14-15 0.13802915403465477 1107 16-17 0.1027354038921473 1107 18-19 0.09559674702743592 1107 20-21 0.10043255460782063 1107 22-23 0.07821121475970472 1107 24-25 0.08969561241401891 1107 26-27 0.11017415662588803 1107 28-29 0.09446573376604306 1107 30-31 0.10314461037044254 1107 32-33 0.129453189675246 1107 34-35 0.11446482491054866 1107 36-37 0.09332706028594373 1107 38-39 0.08555377121305696 1107 40-41 0.11025196476174415 1107 42-43 0.07599967190877521 1107 44-45 0.0766523912344681 1107 46-47 0.0381949624333231 1107 48-49 0.05474919761289243 1107 50-51 0.05710361179029633 1107 52-53 -0.001857853758124861 1107 54-55 0.06762975037140961 1107 56-57 0.029144014947540597 1107 58-59 0.08634584713448135 1107 60-61 0.06107813751965807 1107 62-63 0.07595718987080602 1107 64-65 0.015418975898626286 1107 66-67 0.01620738355467921 1107 68-69 0.044680417811669315 1107 70-71 0.029933554174846222 1107 72-73 0.05557369963527492 1107 74-75 0.006936374104405729 1107 76-77 0.022517330071259778 1107 78-79 0.05822965914246936 1107 80-81 0.05584656778999886 1107 82-83 0.08364400466054178 1107 84-85 0.06902680393623228 1107 86-87 0.07569908104809286 1107 88-89 0.08630916560601776 1107 90-91 0.08399151400686122 1107 92-93 0.07380476202163777 1107 94-95 0.043455471553627945 1107 96-97 0.03873499456767604 1107 98-99 0.020017876359851527 1107 100 0.035836554465564774 1108 1 0.09558843653198679 1108 2 0.12180018439890716 1108 3 0.10992873948261916 1108 4 0.11180346521430806 1108 5 0.089651260369628 1108 6 0.08468121688150632 1108 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-0.3646845828466567 2214 34-35 -0.2879077324275414 2214 36-37 -0.27965767674830033 2214 38-39 -0.2959599322348154 2214 40-41 -0.3063894012288273 2214 42-43 -0.32204790828951957 2214 44-45 -0.28153146672375584 2214 46-47 -0.2542201907876773 2214 48-49 -0.30072841684537366 2214 50-51 -0.29902473474196256 2214 52-53 -0.259680595939102 2214 54-55 -0.20603148629999168 2214 56-57 -0.27442204547941884 2214 58-59 -0.24259863266076565 2214 60-61 -0.26533126878180724 2214 62-63 -0.25403311696178577 2214 64-65 -0.29475221317758127 2214 66-67 -0.2629164482782542 2214 68-69 -0.24364683497515927 2214 70-71 -0.2674073973745408 2214 72-73 -0.252652748616363 2214 74-75 -0.20739109463413286 2214 76-77 -0.201553553973163 2214 78-79 -0.20372154017698563 2214 80-81 -0.15955842369967854 2214 82-83 -0.17534566750154568 2214 84-85 -0.19236559403093878 2214 86-87 -0.139890297954949 2214 88-89 -0.2513003025612832 2214 90-91 -0.21048084392770505 2214 92-93 -0.24147943230241253 2214 94-95 -0.2264607718651206 2214 96-97 -0.27068291167594793 2214 98-99 -0.2033678262494547 2214 100 -0.10992976454449632 2215 1 -0.2572626579170354 2215 2 -0.2551440015534503 2215 3 -0.2842878536441553 2215 4 -0.2767609641343398 2215 5 -0.30081087236287374 2215 6 -0.2584564776444367 2215 7 -0.265399432754144 2215 8 -0.20101448887157858 2215 9 -0.37194484864905064 2215 10-11 -0.33738015749948147 2215 12-13 -0.373741408566616 2215 14-15 -0.43135520064301147 2215 16-17 -0.4291981825642708 2215 18-19 -0.44660793946526667 2215 20-21 -0.45129443389863866 2215 22-23 -0.40136635510835816 2215 24-25 -0.4074149477635345 2215 26-27 -0.41630367633942456 2215 28-29 -0.40354569179692135 2215 30-31 -0.4197814897050307 2215 32-33 -0.41533473884534544 2215 34-35 -0.41588079828895985 2215 36-37 -0.3575471291399168 2215 38-39 -0.41686364166346834 2215 40-41 -0.38430529987128637 2215 42-43 -0.41070645641315195 2215 44-45 -0.3098014829448772 2215 46-47 -0.2599007770736961 2215 48-49 -0.2465286496179857 2215 50-51 -0.2807686142017758 2215 52-53 -0.27332720335249405 2215 54-55 -0.24912268824789408 2215 56-57 -0.23119998018681542 2215 58-59 -0.21501393911239575 2215 60-61 -0.24091807347055294 2215 62-63 -0.2460499969653398 2215 64-65 -0.29106615297360605 2215 66-67 -0.23527726633374613 2215 68-69 -0.21280685873119154 2215 70-71 -0.24890015144378452 2215 72-73 -0.2660753608447948 2215 74-75 -0.26045276113551097 2215 76-77 -0.2730485992908669 2215 78-79 -0.27981412941313266 2215 80-81 -0.2498634789233236 2215 82-83 -0.22412885498152946 2215 84-85 -0.23627286800383018 2215 86-87 -0.18585513584056912 2215 88-89 -0.2605376816010292 2215 90-91 -0.22364480726317026 2215 92-93 -0.2728563130355184 2215 94-95 -0.24548155383060077 2215 96-97 -0.27088251247434414 2215 98-99 -0.2891526244429059 2215 100 -0.19721470351863246 2216 1 0.23694895571205166 2216 2 0.268696521144868 2216 3 0.22632925625907774 2216 4 0.24060229796050692 2216 5 0.2548976680972359 2216 6 0.2524617446244406 2216 7 0.25093894046601406 2216 8 0.30502610103537364 2216 9 0.2860593544579544 2216 10-11 0.21719050741527468 2216 12-13 0.19932322668390867 2216 14-15 0.0959068790819444 2216 16-17 0.13146031941347758 2216 18-19 0.10605151558567627 2216 20-21 0.11155710633725135 2216 22-23 0.10956454009581051 2216 24-25 0.12278734565977345 2216 26-27 0.12124256525449795 2216 28-29 0.09559700588232545 2216 30-31 0.10292131434415097 2216 32-33 0.13854461281385255 2216 34-35 0.16279085130644688 2216 36-37 0.17192035511769177 2216 38-39 0.15098393052447534 2216 40-41 0.11852456586548499 2216 42-43 0.20549001844266002 2216 44-45 0.26155938776636134 2216 46-47 0.3013229363915926 2216 48-49 0.3445075700161766 2216 50-51 0.27900455808567415 2216 52-53 0.33372012538958273 2216 54-55 0.33562019593816217 2216 56-57 0.34940304043619363 2216 58-59 0.3859183168098639 2216 60-61 0.3577688977535445 2216 62-63 0.3376645038393917 2216 64-65 0.3278220761587747 2216 66-67 0.35045293008320044 2216 68-69 0.3279527671369493 2216 70-71 0.3047645234016443 2216 72-73 0.2658025984317973 2216 74-75 0.27280960756375094 2216 76-77 0.29564646920432125 2216 78-79 0.25387208728279376 2216 80-81 0.2881183564885532 2216 82-83 0.24512817297053147 2216 84-85 0.2935236561459149 2216 86-87 0.30499956913463677 2216 88-89 0.288637693281089 2216 90-91 0.2755016630569145 2216 92-93 0.28341460051694867 2216 94-95 0.32311161770374497 2216 96-97 0.341891478240818 2216 98-99 0.28001453050379865 2216 100 0.3100123926290834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 110.0 1 70.0 2 47.0 3 63.0 4 38.0 5 60.0 6 198.0 7 1045.0 8 2830.0 9 3399.0 10 3240.0 11 3693.0 12 4425.0 13 5327.0 14 6507.0 15 7517.0 16 8076.0 17 8953.0 18 9903.0 19 11225.0 20 12757.0 21 14239.0 22 16527.0 23 19517.0 24 24971.0 25 32519.0 26 37540.0 27 36053.0 28 37969.0 29 48195.0 30 56237.0 31 62550.0 32 79830.0 33 110091.0 34 168641.0 35 266864.0 36 524894.0 37 1171998.0 38 1473753.0 39 456827.0 40 635.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.08987837873291 20.340778203464907 13.196893414882464 24.372450002919724 2 15.032928383541286 20.946820813893467 40.316065113676565 23.704185688888682 3 17.935155095668218 26.330112707455243 30.407518539093818 25.32721365778272 4 12.220639749116335 17.60385376256771 40.12102218599099 30.05448430232497 5 13.061917685855198 38.491850509710574 35.6694416001482 12.776790204286026 6 29.647607648419687 38.573661082468774 18.847894400717305 12.93083686839423 7 26.159461676279637 32.749245696787135 23.688789581491495 17.402503045441733 8 24.91981560973547 35.4678480049058 21.431498593812776 18.180837791545958 9 25.944617513539846 15.654289994256718 21.33343511657 37.06765737563343 10-11 24.177966888396092 26.52523124686908 29.336924198309294 19.959877666425534 12-13 24.85303438880586 24.364874158993842 28.497885160599335 22.28420629160096 14-15 22.388113515152096 25.696654790318146 26.05089401445357 25.86433768007619 16-17 22.203783551742585 28.326816652533683 26.881646644349942 22.58775315137379 18-19 21.874563198525518 28.382849659514243 28.02536234565643 21.71722479630381 20-21 23.283596860198124 27.5933969045836 27.236335950469275 21.886670284749002 22-23 23.260877471652854 27.44913330389933 26.83206589495444 22.45792332949338 24-25 22.821360396559864 27.54637307055304 27.241161112224177 22.391105420662917 26-27 22.70761933912617 27.881650223323906 26.826537932288634 22.58419250526129 28-29 22.481097000419215 27.988418460048496 27.033496490594622 22.496988048937663 30-31 22.922670246320035 27.585365417837515 27.093321677467692 22.39864265837476 32-33 22.901166115160006 27.905909326214502 26.61559943360604 22.577325125019453 34-35 22.95777064909598 27.890613327801255 26.99721249720726 22.154403525895503 36-37 22.91547089782378 27.614202709624536 26.891486013046904 22.57884037950478 38-39 22.8635270204781 27.675239293961866 27.123567035777008 22.33766664978302 40-41 22.809863872385716 27.21787767389752 27.334395340562246 22.637863113154527 42-43 22.705361009312806 27.383886040416293 27.062144054752714 22.84860889551819 44-45 22.788571846748145 27.73165781445378 27.092107289158562 22.38766304963951 46-47 22.711687741433042 27.42756572462195 26.92406410936635 22.936682424578663 48-49 22.956435349749373 27.2597365115601 27.140548777553704 22.643279361136827 50-51 22.81176049051186 27.268636379403848 26.99392063447746 22.925682495606832 52-53 22.652454942547724 27.600094799350366 26.836221854592136 22.911228403509774 54-55 22.734781837092083 27.44167166745177 26.917546378271723 22.90600011718442 56-57 22.995829234029745 27.26655439986513 26.83666957240394 22.900946793701184 58-59 22.6843121581372 27.320626932405958 27.264678776146244 22.7303821333106 60-61 22.797467308846958 27.504386265966414 26.942296951237115 22.75584947394951 62-63 22.862195386862993 27.297171873608615 26.771076952103524 23.069555787424868 64-65 22.53482114509528 27.3062101137159 26.910982792963704 23.247985948225114 66-67 22.889131039241366 27.186209261267386 26.826625506512414 23.098034192978837 68-69 22.610718814001533 27.14277283240393 27.043712582703566 23.202795770890965 70-71 22.744803763543214 26.95716092785555 26.89170294407568 23.406332364525557 72-73 22.95871762180673 27.064703241428433 27.04696705373578 22.92961208302905 74-75 22.86072314010034 27.303576884891832 26.892501596252295 22.94319837875553 76-77 22.647461129506336 27.25988469020421 26.818374644409637 23.274279535879817 78-79 22.754295767251893 27.30895605878036 26.725416848095033 23.21133132587271 80-81 22.644021337324897 27.31727794897006 26.991669457193666 23.047031256511378 82-83 23.01065270096488 26.831456842869883 27.438364974706964 22.719525481458273 84-85 23.169433952446415 27.20662811778385 26.766634014971835 22.857303914797903 86-87 22.463854025798163 27.33306266134164 27.153579422047258 23.049503890812936 88-89 23.017168872100825 26.83525283642314 26.961272609344313 23.186305682131728 90-91 22.951746188269002 26.900334314034367 27.03317789967533 23.114741598021297 92-93 23.1672284648948 26.866572497776918 26.98491817121139 22.981280866116883 94-95 22.54988988334499 26.97614260770496 27.254472329006123 23.21949517994393 96-97 22.71401055981576 26.91378152831779 27.09765397122596 23.274553940640487 98-99 22.60436229452267 27.17935468532835 26.87241702488222 23.343865995266754 100 22.87045452286172 26.86809071717687 26.700767285064686 23.560687474896728 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 981.0 1 736.5 2 533.0 3 534.5 4 464.5 5 399.5 6 366.0 7 348.0 8 367.5 9 387.5 10 336.5 11 301.5 12 296.0 13 324.5 14 361.0 15 453.0 16 540.5 17 664.5 18 861.5 19 1080.5 20 1497.0 21 2212.5 22 3359.5 23 5217.5 24 7754.5 25 11054.5 26 16144.5 27 22550.5 28 28699.0 29 36622.5 30 46718.0 31 56525.5 32 69596.0 33 83225.5 34 95102.0 35 107223.0 36 120892.0 37 136543.0 38 144158.5 39 147141.5 40 150233.0 41 151074.0 42 152271.0 43 159115.5 44 170372.5 45 184645.0 46 195917.0 47 205032.0 48 211904.5 49 207766.5 50 200583.5 51 198014.0 52 191662.0 53 181667.0 54 171162.0 55 153188.0 56 135571.0 57 114050.5 58 94990.5 59 85794.5 60 70756.5 61 53278.5 62 39872.0 63 30296.5 64 21803.5 65 13375.0 66 9060.0 67 6482.5 68 4484.0 69 3251.5 70 2503.5 71 1885.0 72 1276.0 73 920.0 74 808.0 75 640.5 76 464.5 77 301.5 78 159.0 79 118.0 80 86.0 81 70.5 82 59.0 83 43.5 84 34.0 85 23.5 86 18.5 87 18.5 88 21.5 89 23.5 90 16.5 91 9.5 92 7.5 93 7.5 94 9.5 95 7.0 96 4.0 97 8.0 98 8.5 99 7.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06041021006556315 2 0.009176769747446417 3 0.011460389868930778 4 0.009472794578009204 5 0.012073584160810837 6 0.010847195577050717 7 0.002833380521100967 8 0.004926698965794965 9 0.006956583518225509 10-11 0.00625881070332751 12-13 0.0052967300039984495 14-15 0.009863970246967172 16-17 0.009367071424236779 18-19 0.00748519928708763 20-21 0.006523118587758571 22-23 0.0023047647522388462 24-25 0.003393713236094815 26-27 0.0045566679275914805 28-29 0.005402453157770874 30-31 0.008108965894344932 32-33 0.009916831823853384 34-35 0.011597829968834928 36-37 0.01807865929508453 38-39 0.018850438317623227 40-41 0.01830067791800662 42-43 0.02464406714435207 44-45 0.028016635749692397 46-47 0.03052227449409885 48-49 0.03166408455484103 50-51 0.03249929746964318 52-53 0.030712576170889214 54-55 0.03683394677431257 56-57 0.04025937695653911 58-59 0.03683394677431257 60-61 0.037901750627414056 62-63 0.03328164880755912 64-65 0.035396111883007605 66-67 0.03771144895062369 68-69 0.04032281084880257 70-71 0.039603893403150084 72-73 0.0361678909055463 74-75 0.039191573103437634 76-77 0.03821892008873133 78-79 0.03809205230420442 80-81 0.0429236004316042 82-83 0.03904356068815624 84-85 0.03501550852942688 86-87 0.03369396910727157 88-89 0.03179095233936794 90-91 0.033672824476517094 92-93 0.03503665316018136 94-95 0.03904356068815624 96-97 0.033810264576421244 98-99 0.03171694613172724 100 0.028841276349117304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4729333.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.39217534227439 #Duplication Level Percentage of deduplicated Percentage of total 1 68.08086001475189 34.988234953309664 2 14.81694733398988 15.22950310851306 3 6.584619399219766 10.15193744180531 4 3.480362289533187 7.154535561533164 5 2.0491222262976496 5.265442437582023 6 1.2903479486075962 3.978827281643139 7 0.8639311405280627 3.1079510460368507 8 0.6047877129784743 2.486508495219429 9 0.4424512625796082 2.046467958021172 >10 1.7511612773039316 13.974575401621788 >50 0.028879343449777783 0.9918314515613922 >100 0.00628879127015663 0.5050732439957748 >500 8.041983002176922E-5 0.023185849530712874 >1k 1.6083966004269804E-4 0.09592576962656578 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.114463075448483E-5 2 0.0 0.0 0.0 0.0 2.114463075448483E-5 3 0.0 0.0 0.0 0.0 2.114463075448483E-5 4 0.0 0.0 0.0 0.0 2.114463075448483E-5 5 0.0 0.0 0.0 0.0 2.114463075448483E-5 6 0.0 0.0 0.0 0.0 2.114463075448483E-5 7 0.0 0.0 0.0 0.0 2.114463075448483E-5 8 0.0 0.0 0.0 0.0 2.114463075448483E-5 9 0.0 0.0 0.0 0.0 2.114463075448483E-5 10-11 0.0 0.0 0.0 0.0 2.114463075448483E-5 12-13 0.0 0.0 0.0 1.0572315377242415E-5 5.286157688621207E-5 14-15 0.0 0.0 0.0 2.114463075448483E-5 6.343389226345449E-5 16-17 0.0 0.0 0.0 4.228926150896966E-5 6.343389226345449E-5 18-19 0.0 0.0 0.0 7.40062076406969E-5 8.457852301793932E-5 20-21 0.0 0.0 0.0 1.0572315377242415E-4 8.457852301793932E-5 22-23 0.0 0.0 0.0 2.0087399216760587E-4 8.457852301793932E-5 24-25 0.0 0.0 0.0 5.497603996166055E-4 8.457852301793932E-5 26-27 0.0 0.0 0.0 0.0013321117375325441 8.457852301793932E-5 28-29 0.0 0.0 0.0 0.004260643097028693 8.457852301793932E-5 30-31 0.0 0.0 0.0 0.012908797075612988 8.457852301793932E-5 32-33 0.0 0.0 0.0 0.03397942162245712 8.457852301793932E-5 34-35 1.0572315377242415E-5 0.0 0.0 0.06797998787566872 8.457852301793932E-5 36-37 2.114463075448483E-5 0.0 0.0 0.12209967029177266 8.457852301793932E-5 38-39 2.114463075448483E-5 0.0 0.0 0.19723711567783447 8.457852301793932E-5 40-41 2.114463075448483E-5 0.0 0.0 0.2763603239611167 8.457852301793932E-5 42-43 2.114463075448483E-5 0.0 0.0 0.35887724548049377 8.457852301793932E-5 44-45 2.114463075448483E-5 0.0 0.0 0.4585530348571352 8.457852301793932E-5 46-47 2.114463075448483E-5 0.0 0.0 0.5717508156012698 8.457852301793932E-5 48-49 2.114463075448483E-5 0.0 0.0 0.6917148781868394 8.457852301793932E-5 50-51 2.114463075448483E-5 0.0 0.0 0.8119855379183492 8.457852301793932E-5 52-53 2.114463075448483E-5 0.0 0.0 0.9296236911209255 9.515083839518174E-5 54-55 2.114463075448483E-5 0.0 0.0 1.0563328909171759 1.0572315377242415E-4 56-57 2.114463075448483E-5 0.0 0.0 1.2076227239655148 1.1629546914966655E-4 58-59 2.114463075448483E-5 0.0 0.0 1.3723288252275743 1.2686778452690898E-4 60-61 2.114463075448483E-5 0.0 0.0 1.5367811909205802 1.2686778452690898E-4 62-63 2.114463075448483E-5 0.0 0.0 1.7025868129818729 2.114463075448483E-4 64-65 2.114463075448483E-5 0.0 0.0 1.8742600700775354 2.114463075448483E-4 66-67 2.114463075448483E-5 0.0 0.0 2.06605244333609 2.114463075448483E-4 68-69 2.114463075448483E-5 0.0 0.0 2.2764098023970822 2.114463075448483E-4 70-71 2.114463075448483E-5 0.0 0.0 2.5001940019871722 2.114463075448483E-4 72-73 2.114463075448483E-5 0.0 0.0 2.7219588893402094 2.4316325367657553E-4 74-75 2.114463075448483E-5 0.0 0.0 2.9450558884307787 2.854525151855452E-4 76-77 2.114463075448483E-5 0.0 0.0 3.1910630949438326 2.960248305627876E-4 78-79 2.114463075448483E-5 1.0572315377242415E-5 0.0 3.4582995107343892 3.0659714594003E-4 80-81 2.114463075448483E-5 2.114463075448483E-5 0.0 3.735008721102955 3.2774177669451485E-4 82-83 2.114463075448483E-5 2.114463075448483E-5 0.0 4.016845504429483 3.594587228262421E-4 84-85 2.114463075448483E-5 2.114463075448483E-5 0.0 4.303820433029351 3.594587228262421E-4 86-87 2.114463075448483E-5 2.114463075448483E-5 0.0 4.606326092918389 3.8060335358072695E-4 88 2.114463075448483E-5 2.114463075448483E-5 0.0 4.845820753159061 4.0174798433521174E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6460 0.0 35.80738 1 CAACGCA 6165 0.0 34.681496 5 GGTATCA 4295 0.0 32.94908 1 ATCAACG 6565 0.0 32.85539 3 TATCAAC 7865 0.0 29.03766 2 AACGCAG 7725 0.0 29.013052 6 ACGCAGA 8025 0.0 26.580395 7 CGCAGAG 8210 0.0 25.92641 8 GTACTAG 2480 0.0 25.78263 1 TCAACGC 8535 0.0 25.546919 4 GTACATG 14080 0.0 25.17727 1 TACATGG 13610 0.0 25.136068 2 ACATGGG 14315 0.0 23.340326 3 GCATCGT 2405 0.0 23.054422 8 CATGGGG 10625 0.0 22.998446 4 CGTTAAC 555 0.0 21.165783 9 ATGGGGG 7595 0.0 20.479517 5 GCAGAGT 10175 0.0 20.31915 9 GTGATCG 1560 0.0 19.57835 8 TGATCGC 1600 0.0 19.382566 9 >>END_MODULE