FastQCFastQC Report
Mon 16 Jan 2017
ERR1146529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4729333
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123460.2610516112948697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102320.21635186187988878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56310.11906541577850406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78400.053.6554641
GTATCAA144300.044.7383841
ATCAACG173450.036.407123
CAACGCA174150.036.2873955
TATCAAC183450.035.033922
AACGCAG193450.033.2599686
TCAACGC192850.032.8418774
ACGCAGA206550.030.580057
CGCAGAG208650.030.2722728
GCAGAGT237550.026.3322019
GTGGTAT22450.025.9894941
GTACTAG19850.025.126841
TACATGG134950.024.4051172
GTACATG140650.024.0536861
ACATGGG139150.023.2482263
GAGTACT161700.022.75704612-13
CAGAGTA228150.022.4939810-11
CTAACGC10200.022.127163
AGAGTAC219200.022.0402610-11
TAACGCC10300.021.9121024
Produced by FastQC (version 0.11.5)