##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146529_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4729333
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.421060221388515	34.0	31.0	34.0	31.0	34.0
2	32.55203640767102	34.0	31.0	34.0	31.0	34.0
3	32.67542188295897	34.0	31.0	34.0	31.0	34.0
4	36.15327319941311	37.0	35.0	37.0	35.0	37.0
5	36.18102468149314	37.0	37.0	37.0	35.0	37.0
6	36.26354625483128	37.0	37.0	37.0	35.0	37.0
7	36.245928971379264	37.0	37.0	37.0	35.0	37.0
8	36.25576270480425	37.0	37.0	37.0	35.0	37.0
9	38.065399074245775	39.0	38.0	39.0	35.0	39.0
10-11	38.02990136241199	39.0	38.0	39.0	35.0	39.0
12-13	38.00927530372677	39.0	38.0	39.0	35.0	39.0
14-15	39.46136770238002	41.0	39.0	41.0	36.5	41.0
16-17	39.45191668677168	41.0	39.0	41.0	36.5	41.0
18-19	39.495198582971426	41.0	39.0	41.0	37.0	41.0
20-21	39.527668806573786	41.0	39.0	41.0	37.0	41.0
22-23	39.487781786564824	41.0	39.0	41.0	36.5	41.0
24-25	39.4761462134301	41.0	39.0	41.0	36.0	41.0
26-27	39.40490709789309	41.0	39.0	41.0	36.0	41.0
28-29	39.3220160221325	41.0	39.0	41.0	36.0	41.0
30-31	39.231663217624984	41.0	39.0	41.0	36.0	41.0
32-33	39.12728824550946	41.0	39.0	41.0	35.0	41.0
34-35	39.057579261177	41.0	39.0	41.0	35.0	41.0
36-37	38.9809793474048	41.0	39.0	41.0	35.0	41.0
38-39	38.880840384891485	40.0	39.0	41.0	35.0	41.0
40-41	38.79058558574751	40.0	38.0	41.0	35.0	41.0
42-43	38.7018068932765	40.0	38.0	41.0	35.0	41.0
44-45	38.553288486981145	40.0	38.0	41.0	35.0	41.0
46-47	38.33641551567631	40.0	38.0	41.0	34.5	41.0
48-49	38.141310201671146	40.0	38.0	41.0	34.0	41.0
50-51	37.99840738641157	40.0	38.0	41.0	34.0	41.0
52-53	37.87014765084209	40.0	37.0	41.0	33.0	41.0
54-55	37.73179822609235	40.0	37.0	41.0	33.0	41.0
56-57	37.5335713302489	40.0	36.5	41.0	33.0	41.0
58-59	37.324869807222285	39.5	36.0	41.0	33.0	41.0
60-61	37.14511792677742	39.0	35.5	41.0	33.0	41.0
62-63	36.893803312221834	39.0	35.0	41.0	32.5	41.0
64-65	36.604606928714894	38.5	35.0	41.0	32.0	41.0
66-67	36.34708689364864	37.5	35.0	40.0	32.0	41.0
68-69	36.035754619097446	37.0	35.0	40.0	31.5	41.0
70-71	35.76282702021617	36.5	35.0	39.5	31.0	41.0
72-73	35.437830345209356	36.0	35.0	39.0	31.0	41.0
74-75	35.1104433754189	36.0	35.0	39.0	31.0	40.5
76-77	34.8032698268445	35.0	35.0	37.5	31.0	39.5
78-79	34.51345929753731	35.0	35.0	37.0	31.0	39.0
80-81	34.25371484731568	35.0	35.0	36.5	31.0	39.0
82-83	33.99744572860486	35.0	34.0	36.0	30.5	38.0
84-85	33.79017738865079	35.0	34.0	36.0	30.0	37.0
86-87	33.603936326750514	35.0	34.0	35.5	30.0	37.0
88-89	33.45664769217985	35.0	34.0	35.0	30.0	36.0
90-91	33.36985437481353	35.0	34.0	35.0	30.0	36.0
92-93	33.28367372312333	35.0	34.0	35.0	30.0	36.0
94-95	33.212970623975934	35.0	34.0	35.0	30.0	36.0
96-97	33.16676495395862	35.0	34.0	35.0	30.0	35.5
98-99	33.11607567071297	35.0	34.0	35.0	30.0	35.0
100	32.5652890164427	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33931076350400247
1101	2	0.3299309237523431
1101	3	0.31155609634492265
1101	4	0.20923016162284114
1101	5	0.2307100960857298
1101	6	0.2259332783872452
1101	7	0.22535612354137413
1101	8	0.22483668870914642
1101	9	0.2659114290352278
1101	10-11	0.3178303132915943
1101	12-13	0.3305311249114098
1101	14-15	0.45901395614220064
1101	16-17	0.4201920056052657
1101	18-19	0.4249800604235219
1101	20-21	0.38417255094088887
1101	22-23	0.39280813577945395
1101	24-25	0.39673668046492594
1101	26-27	0.3905594135752324
1101	28-29	0.4190004883113474
1101	30-31	0.43357824854295046
1101	32-33	0.3941780138485953
1101	34-35	0.41874459226760763
1101	36-37	0.45750470543644894
1101	38-39	0.4363785676508556
1101	40-41	0.44279787184621
1101	42-43	0.39381390194493093
1101	44-45	0.4005726067232587
1101	46-47	0.40215819556394905
1101	48-49	0.39716786758934575
1101	50-51	0.36618101935464153
1101	52-53	0.39814869691581833
1101	54-55	0.4208783844401296
1101	56-57	0.39484108151037844
1101	58-59	0.3524916350787137
1101	60-61	0.31931679549324343
1101	62-63	0.37018741058868443
1101	64-65	0.4099381681248033
1101	66-67	0.36481903729497134
1101	68-69	0.3740531616708722
1101	70-71	0.36099699955577336
1101	72-73	0.3284575898782478
1101	74-75	0.32485144116393627
1101	76-77	0.3129211209933871
1101	78-79	0.32902572267500574
1101	80-81	0.2815990389265721
1101	82-83	0.2983163693588864
1101	84-85	0.2939198951094113
1101	86-87	0.3251614124476774
1101	88-89	0.27308304402129124
1101	90-91	0.26667187718689433
1101	92-93	0.27123381507048094
1101	94-95	0.24716971132140486
1101	96-97	0.24262035404074567
1101	98-99	0.2249029778530769
1101	100	0.2634193898003474
1102	1	0.3067594261713893
1102	2	0.32220010425158563
1102	3	0.3025154540086703
1102	4	0.19271354614188851
1102	5	0.1984823699079783
1102	6	0.1995672498436818
1102	7	0.24621667359547672
1102	8	0.20285167732657072
1102	9	0.2916060364374715
1102	10-11	0.2693646182213101
1102	12-13	0.32133982491854596
1102	14-15	0.36864276380970296
1102	16-17	0.355286136102805
1102	18-19	0.34793715250059165
1102	20-21	0.3109599117445683
1102	22-23	0.34458864646223475
1102	24-25	0.371315615009145
1102	26-27	0.36455211149422695
1102	28-29	0.3866501864367038
1102	30-31	0.39615704422033104
1102	32-33	0.36014968630031063
1102	34-35	0.3342487913820804
1102	36-37	0.36730425501929886
1102	38-39	0.38474678591866507
1102	40-41	0.36491355949098647
1102	42-43	0.27871500111449166
1102	44-45	0.311479014977607
1102	46-47	0.2713173761724832
1102	48-49	0.2972599697310443
1102	50-51	0.33175953151224036
1102	52-53	0.24854691684873842
1102	54-55	0.23443068162544023
1102	56-57	0.2324804867745911
1102	58-59	0.24675539334241847
1102	60-61	0.23527110316171473
1102	62-63	0.19671489754259852
1102	64-65	0.24653553209800094
1102	66-67	0.20962503839948
1102	68-69	0.2473845191520354
1102	70-71	0.1639509928451588
1102	72-73	0.1523565976875787
1102	74-75	0.15142317263405403
1102	76-77	0.21075355115655015
1102	78-79	0.1778894255205472
1102	80-81	0.17059002351170705
1102	82-83	0.16617917664193982
1102	84-85	0.15233091136389731
1102	86-87	0.1586870291996476
1102	88-89	0.18379438294829953
1102	90-91	0.12434729782161469
1102	92-93	0.14175859669273905
1102	94-95	0.14626980280635848
1102	96-97	0.17500456256729535
1102	98-99	0.22948695917806106
1102	100	0.165280269663171
1103	1	0.24424605622774465
1103	2	0.26603521426199706
1103	3	0.2613344380467737
1103	4	0.16752803336673594
1103	5	0.09346842312112358
1103	6	0.0829781878163729
1103	7	0.10336758090574705
1103	8	0.1388506415725459
1103	9	0.24101381074564188
1103	10-11	0.21012888419473796
1103	12-13	0.2061161892168215
1103	14-15	0.29290312263182017
1103	16-17	0.30200980769699726
1103	18-19	0.31765479416046105
1103	20-21	0.3274634856086962
1103	22-23	0.3543889433442118
1103	24-25	0.31787933439159133
1103	26-27	0.279916035463728
1103	28-29	0.30096047424300565
1103	30-31	0.2905230166241637
1103	32-33	0.32484385685105366
1103	34-35	0.34600115131173226
1103	36-37	0.360433770419732
1103	38-39	0.39508253430222595
1103	40-41	0.33041882286477176
1103	42-43	0.33236027843507543
1103	44-45	0.320252210483865
1103	46-47	0.36964182612044283
1103	48-49	0.39468354441557096
1103	50-51	0.37236666539626384
1103	52-53	0.37252360981945287
1103	54-55	0.4113635934786757
1103	56-57	0.44377200025734
1103	58-59	0.44909427462552287
1103	60-61	0.4320142848750166
1103	62-63	0.4108707580131963
1103	64-65	0.4140504274714587
1103	66-67	0.39311861877553156
1103	68-69	0.3796444626470361
1103	70-71	0.3055893044635525
1103	72-73	0.33600090078407163
1103	74-75	0.3241543457536764
1103	76-77	0.31055557856814175
1103	78-79	0.2686919169704467
1103	80-81	0.3148010942865227
1103	82-83	0.33344684983590156
1103	84-85	0.33356541991912536
1103	86-87	0.260621786951134
1103	88-89	0.28292542634229534
1103	90-91	0.2981123534292962
1103	92-93	0.2449190052515604
1103	94-95	0.24786623560797238
1103	96-97	0.2552923816897774
1103	98-99	0.23914048151087286
1103	100	0.2847711606680079
1104	1	0.2563367559500236
1104	2	0.27887745499896965
1104	3	0.25347492439778563
1104	4	0.18108726929439456
1104	5	0.16334412589156955
1104	6	0.15749793312559035
1104	7	0.19599170879303074
1104	8	0.2002886961684922
1104	9	0.17113573718335573
1104	10-11	0.21741991582787534
1104	12-13	0.23291697496497932
1104	14-15	0.29206216889612335
1104	16-17	0.31459955910253257
1104	18-19	0.34004031878283314
1104	20-21	0.30458720707452613
1104	22-23	0.352312637715265
1104	24-25	0.3419935049531304
1104	26-27	0.3224040121622309
1104	28-29	0.36060129927857787
1104	30-31	0.36433575793689954
1104	32-33	0.3766754628931821
1104	34-35	0.3453415631521395
1104	36-37	0.39562106297544375
1104	38-39	0.41770937166590016
1104	40-41	0.3923679524454826
1104	42-43	0.36411415637316225
1104	44-45	0.3830456806457505
1104	46-47	0.41123889311224104
1104	48-49	0.3545523041527545
1104	50-51	0.4006910235890473
1104	52-53	0.4239472703220599
1104	54-55	0.35871609635749024
1104	56-57	0.35470676961315917
1104	58-59	0.3731236047074802
1104	60-61	0.3550548254155572
1104	62-63	0.33803562965174905
1104	64-65	0.3301083086609182
1104	66-67	0.2864836191142146
1104	68-69	0.30893051561729834
1104	70-71	0.2728794893853106
1104	72-73	0.31465716983507974
1104	74-75	0.29919718934915096
1104	76-77	0.2551806547721682
1104	78-79	0.2631268218000926
1104	80-81	0.26180830826741897
1104	82-83	0.28483867399088325
1104	84-85	0.23088286894709853
1104	86-87	0.24765626503824478
1104	88-89	0.26881040906938125
1104	90-91	0.26372452575199645
1104	92-93	0.25070746288211865
1104	94-95	0.19812583731494016
1104	96-97	0.22130657257238795
1104	98-99	0.22296507678283461
1104	100	0.24987242734822246
1105	1	0.2746189005191937
1105	2	0.25274865171274286
1105	3	0.24378990514153998
1105	4	0.16013138094695023
1105	5	0.13634417698068546
1105	6	0.1385941752488975
1105	7	0.18289656671635157
1105	8	0.1669223686828687
1105	9	0.21136398598955708
1105	10-11	0.21182984190082266
1105	12-13	0.22194637947234241
1105	14-15	0.2803514876824664
1105	16-17	0.2839655430953343
1105	18-19	0.26780532186448625
1105	20-21	0.27245651892578593
1105	22-23	0.27251059175898007
1105	24-25	0.270319466597158
1105	26-27	0.2795008347449297
1105	28-29	0.33197657496960886
1105	30-31	0.3399108420980781
1105	32-33	0.27994073174696865
1105	34-35	0.2661402165733193
1105	36-37	0.25553921167957583
1105	38-39	0.29480728125119526
1105	40-41	0.2641367448182024
1105	42-43	0.27041144506256387
1105	44-45	0.28031177127351015
1105	46-47	0.2778864792599194
1105	48-49	0.27469206007718583
1105	50-51	0.2888922842946613
1105	52-53	0.2548435691869102
1105	54-55	0.25648830626638386
1105	56-57	0.2954201694203249
1105	58-59	0.25262742063011245
1105	60-61	0.22744830731316057
1105	62-63	0.3061889861527334
1105	64-65	0.29037004801725885
1105	66-67	0.22647447742233595
1105	68-69	0.2716356194206142
1105	70-71	0.24621688086168803
1105	72-73	0.30509211218589627
1105	74-75	0.26048398302845044
1105	76-77	0.27020384597265945
1105	78-79	0.255570892798751
1105	80-81	0.2631963453689963
1105	82-83	0.23553563831872282
1105	84-85	0.19811352329005416
1105	86-87	0.19644052871898765
1105	88-89	0.18219924837887191
1105	90-91	0.21712940921987922
1105	92-93	0.20355952943711486
1105	94-95	0.18933971485974865
1105	96-97	0.19427579626774616
1105	98-99	0.1666044484518494
1105	100	0.22556663612446215
1106	1	0.15104585088100464
1106	2	0.15430844893625562
1106	3	0.1629507991747019
1106	4	0.11326926670437132
1106	5	0.09090145108114456
1106	6	0.0748176677663892
1106	7	0.07165546114735122
1106	8	0.07972881317051161
1106	9	0.11615952200960322
1106	10-11	0.10040726852839299
1106	12-13	0.07513485900669536
1106	14-15	0.1610939815039103
1106	16-17	0.10879949768077068
1106	18-19	0.13244895390963762
1106	20-21	0.11192433738960261
1106	22-23	0.15593950694489678
1106	24-25	0.11749167625971779
1106	26-27	0.1237507809694236
1106	28-29	0.1575220716465111
1106	30-31	0.18420803932589536
1106	32-33	0.16830419195662927
1106	34-35	0.12527994810337617
1106	36-37	0.1963965234776026
1106	38-39	0.21769747388373872
1106	40-41	0.23644498306830997
1106	42-43	0.21554485393969713
1106	44-45	0.24478535545402735
1106	46-47	0.2597702257282748
1106	48-49	0.2491217826905725
1106	50-51	0.30050226978508476
1106	52-53	0.3266513741978585
1106	54-55	0.31121143066584267
1106	56-57	0.283620570825704
1106	58-59	0.22056704192090848
1106	60-61	0.29506217025580384
1106	62-63	0.27553091026975096
1106	64-65	0.2560892850951504
1106	66-67	0.20051417536774352
1106	68-69	0.2311218121963634
1106	70-71	0.20604535177049144
1106	72-73	0.20096260780876207
1106	74-75	0.1696462984114362
1106	76-77	0.17987725058903692
1106	78-79	0.17827463134108967
1106	80-81	0.14174125975496565
1106	82-83	0.11877149232635276
1106	84-85	0.14190279236318304
1106	86-87	0.1546120864684255
1106	88-89	0.13602145296017198
1106	90-91	0.10408953424580858
1106	92-93	0.044492954889776115
1106	94-95	0.051577654518617067
1106	96-97	0.09320293730598905
1106	98-99	0.11963801448269606
1106	100	0.06167025954654548
1107	1	0.15992346003872626
1107	2	0.1488518729817585
1107	3	0.15223873394967313
1107	4	0.10420056920617071
1107	5	0.0847311133826949
1107	6	0.1072111408157781
1107	7	0.10975870820138312
1107	8	0.15637319472937605
1107	9	0.15188176354863003
1107	10-11	0.13052011943125308
1107	12-13	0.1519598706387697
1107	14-15	0.19095201462498324
1107	16-17	0.1922416129911042
1107	18-19	0.19627371942249283
1107	20-21	0.19697852561169782
1107	22-23	0.1892756838071179
1107	24-25	0.14605860329125164
1107	26-27	0.18539072846292015
1107	28-29	0.2344641838773569
1107	30-31	0.1956079105952142
1107	32-33	0.1818708442336785
1107	34-35	0.17901960451710153
1107	36-37	0.2140425998756612
1107	38-39	0.22900738005848353
1107	40-41	0.23850216223486598
1107	42-43	0.1987523761174259
1107	44-45	0.2426141551784866
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2216	52-53	-0.9814658691700231
2216	54-55	-0.9956207510056743
2216	56-57	-0.9846053656200269
2216	58-59	-0.9655625775313297
2216	60-61	-0.9507718479111205
2216	62-63	-0.8845505999081595
2216	64-65	-0.8496678362468089
2216	66-67	-0.9190196933627774
2216	68-69	-0.9229409024383273
2216	70-71	-0.8733027494285039
2216	72-73	-0.8078181180349446
2216	74-75	-0.8206827990834711
2216	76-77	-0.7949402769277114
2216	78-79	-0.8044084061298733
2216	80-81	-0.7644642349788029
2216	82-83	-0.7060772521881944
2216	84-85	-0.820237983884283
2216	86-87	-0.7772586884293418
2216	88-89	-0.758497677580813
2216	90-91	-0.7120666567497125
2216	92-93	-0.7695276966951425
2216	94-95	-0.786120048378308
2216	96-97	-0.748952247554854
2216	98-99	-0.7450245651541749
2216	100	-0.8907380148411654
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	12.0
8	101.0
9	163.0
10	259.0
11	250.0
12	201.0
13	227.0
14	393.0
15	742.0
16	1556.0
17	2956.0
18	5579.0
19	8782.0
20	12719.0
21	18682.0
22	27175.0
23	32077.0
24	30193.0
25	24980.0
26	21872.0
27	24259.0
28	30635.0
29	37828.0
30	45421.0
31	58403.0
32	76425.0
33	102247.0
34	152296.0
35	242962.0
36	454149.0
37	1117382.0
38	1615107.0
39	581909.0
40	1391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16299028029913	20.280901786932063	13.613354042192363	23.942753890576444
2	15.256657663584965	21.17695806445947	39.97160886685393	23.594775405101643
3	17.694916788125465	26.69846550651226	30.84769394624547	24.75892375911681
4	12.329772507032176	18.033367496008424	39.55475328127666	30.082106715682738
5	12.658421436611015	38.33866983846222	35.67861751906557	13.324291205861194
6	29.161770660757274	38.95113227515465	18.425059656030253	13.46203740805782
7	25.32123465242758	33.41316139640046	23.661718750495577	17.603885200676377
8	25.0838543194146	34.86950485406716	21.268474856813846	18.778165969704396
9	26.04872611000325	14.899331470209434	21.346350531882614	37.7055918879047
10-11	24.122027355654595	26.28143334377173	29.28500699781555	20.311532302758124
12-13	25.27780344501011	24.209777573285702	28.74728846541362	21.76513051629056
14-15	22.475750003092404	25.236436743001995	26.963531095997627	25.324282157907973
16-17	21.532825030506416	27.9792837594646	27.75420973739849	22.733681472630497
18-19	21.218552383602507	27.19951206650071	30.110144496063185	21.471791053833595
20-21	22.549162852351483	26.416114069362422	29.648292898808354	21.38643017947774
22-23	22.435643089123264	26.326729092270234	29.308982632665508	21.928645185940994
24-25	22.078916836687117	26.386395290836994	29.746118532993975	21.78856933948191
26-27	22.026848183454202	26.735112541239957	29.23600431604203	22.00203495926381
28-29	21.753789648850894	26.866818344247495	29.526708857279992	21.852683149621615
30-31	22.251903441672898	26.382342269345667	29.497478288670187	21.868276000311248
32-33	22.34388554240751	26.74300696990281	29.012018258660838	21.901089229028837
34-35	22.319629651596813	26.80254580166755	29.28000720617625	21.597817340559384
36-37	22.30187578519821	26.55691397848667	29.085681716710898	22.055528519604227
38-39	22.32676073194272	26.571761238888296	29.275044565220952	21.826433463948028
40-41	22.19575939229586	26.24500584127588	29.422258168535347	22.136976597892914
42-43	22.283564288521248	26.327591020970615	28.946250810240016	22.44259388026812
44-45	22.39644895344141	26.800622078062126	28.754078290490583	22.048850678005884
46-47	22.55034685237352	26.58016722286038	28.23527919197643	22.634206732789668
48-49	22.82817936846521	26.608460779852592	28.19252050618865	22.370839345493543
50-51	22.655932167551224	26.760154779777135	27.868035438648427	22.715877614023217
52-53	22.44975634232579	27.10389121723324	27.748524431369393	22.697828009071575
54-55	22.516712147616033	26.967589814875215	27.729578532160197	22.786119505348555
56-57	22.806091438393494	26.791792473865282	27.59771623393642	22.804399853804803
58-59	22.301454095832778	26.9654927786832	28.096586598974145	22.636466526509878
60-61	22.548160644059774	27.148783935213412	27.699547740175746	22.603507680551072
62-63	22.575614399706005	26.932990589921406	27.540227488644703	22.951167521727886
64-65	22.241484637023014	26.92046463524256	27.661404457353562	23.176646270380868
66-67	22.532097520850765	26.842498402194476	27.60470846458951	23.020695612365245
68-69	22.302794154542553	26.691306615432943	27.872093587541087	23.13380564248342
70-71	22.456099396459873	26.44591768315697	27.716147315131344	23.381835605251812
72-73	22.622992036340055	26.48809807010076	27.849221850975248	23.03968804258394
74-75	22.570364117630394	26.778361011810485	27.544380787729644	23.106894082829477
76-77	22.36644285027364	26.74314795776176	27.443576827581307	23.446832364383294
78-79	22.38012441167285	26.814819701609053	27.367882639689306	23.437173247028788
80-81	22.230298299030213	26.920390334236455	27.5542431314024	23.295068235330934
82-83	22.625503917102748	26.405018306525598	27.980380707646514	22.989097068725144
84-85	22.795097530189526	26.67743351833838	27.33768553498217	23.189783416489924
86-87	22.076959722210184	26.90681832166114	27.68066984526987	23.33555211085881
88-89	22.51692128190373	26.400064871830033	27.59454810204965	23.48846574421659
90-91	22.416609532106275	26.492343264086422	27.614288062850306	23.476759140956997
92-93	22.591018648929985	26.47002230547098	27.506320658748283	23.432638386850748
94-95	22.020274531342427	26.50772598458662	27.808744680142496	23.66325480392846
96-97	22.177228583320975	26.44559920521184	27.667521512913986	23.709650698553205
98-99	22.004566419562106	26.645986399275834	27.500191888579025	23.849255292583035
100	22.18894742462159	26.366051671440093	27.3828395504202	24.062161353518118
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	179.5
2	226.5
3	358.0
4	253.5
5	217.0
6	406.5
7	1031.5
8	2038.0
9	2468.0
10	1841.0
11	1310.0
12	1377.0
13	1571.0
14	1915.5
15	2373.0
16	2853.5
17	3468.5
18	4224.0
19	5006.0
20	5919.5
21	7092.5
22	8588.5
23	10491.5
24	13120.0
25	16513.0
26	21331.5
27	27362.5
28	33036.0
29	40676.5
30	50181.5
31	58655.5
32	70391.0
33	83320.0
34	94304.5
35	104956.5
36	117923.0
37	133563.0
38	140748.0
39	144227.5
40	148385.0
41	148998.0
42	149585.0
43	157044.0
44	168668.5
45	182318.0
46	193590.0
47	202717.5
48	209800.5
49	205238.0
50	197482.5
51	193228.5
52	185951.0
53	177297.5
54	168891.5
55	151776.5
56	133406.5
57	112225.0
58	93803.0
59	84942.5
60	69879.0
61	51813.0
62	37932.0
63	28622.0
64	20487.5
65	12260.0
66	8067.0
67	5718.0
68	3896.5
69	2757.5
70	2057.5
71	1464.0
72	933.0
73	678.5
74	609.0
75	503.5
76	328.5
77	167.0
78	68.0
79	38.5
80	21.5
81	14.5
82	9.5
83	3.0
84	2.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005878207349746783
2	0.08276008477305362
3	4.228926150896966E-4
4	0.0
5	0.03873696354221621
6	0.0029179590441189064
7	4.228926150896966E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.286157688621207E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.968988227303935E-4
24-25	0.0
26-27	0.0
28-29	6.343389226345449E-5
30-31	1.0572315377242415E-4
32-33	0.0014906964681911804
34-35	0.0011946716376283928
36-37	7.083451302752417E-4
38-39	6.026219765028176E-4
40-41	0.00122638858376012
42-43	5.180434534848784E-4
44-45	4.863265073531511E-4
46-47	3.2774177669451485E-4
48-49	6.1319429188006E-4
50-51	6.977728148979994E-4
52-53	0.0011523823761194231
54-55	0.0013426840529097866
56-57	8.352129148021508E-4
58-59	0.0022307585445981493
60-61	0.0017444320372449985
62-63	8.880744916883627E-4
64-65	0.004313504673914905
66-67	0.00402805215872936
68-69	0.003309134713076876
70-71	0.001480124152813938
72-73	0.004778686550513571
74-75	0.0031082607209092696
76-77	0.0033619962899630877
78-79	7.189174456524842E-4
80-81	3.1716946131727244E-5
82-83	1.7972936141312106E-4
84-85	5.286157688621207E-5
86-87	4.228926150896966E-5
88-89	1.585847306586362E-4
90-91	1.0572315377242415E-5
92-93	0.0
94-95	1.0572315377242415E-5
96-97	4.7575419197590864E-4
98-99	5.497603996166056E-4
100	5.920496611255753E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4729333.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.53317091590786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.85320471315121	30.82678464074578
2	16.45029994239723	15.638698375598334
3	7.140510770945367	10.182333567066816
4	3.8007394930029346	7.226447997109981
5	2.2556400422486593	5.360886182648558
6	1.4298717244862706	4.077980224069789
7	0.9499404862114156	3.160757844371948
8	0.6376376916570168	2.424715310396635
9	0.4946469175515931	2.1160922827506172
>10	1.9326413021536277	14.46642108233766
>50	0.03769731485127644	1.1877892371507983
>100	0.015178613904736628	1.297488379298468
>500	0.0012119053974847527	0.41908151400570437
>1k	5.193880274928558E-4	0.4538095255733803
>5k	8.65646712488093E-5	0.23169838049720776
>10k+	1.731293424976186E-4	0.9290154563782556
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12346	0.2610516112948697	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10232	0.21635186187988878	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5631	0.11906541577850406	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	4.228926150896966E-5
14-15	0.0	0.0	0.0	0.0	6.343389226345449E-5
16-17	0.0	0.0	0.0	2.114463075448483E-5	8.457852301793932E-5
18-19	0.0	0.0	0.0	5.286157688621207E-5	8.457852301793932E-5
20-21	0.0	0.0	0.0	8.457852301793932E-5	8.457852301793932E-5
22-23	0.0	0.0	0.0	1.7972936141312106E-4	1.0572315377242415E-4
24-25	0.0	0.0	0.0	5.286157688621207E-4	1.0572315377242415E-4
26-27	0.0	0.0	0.0	0.0013638286836642716	1.0572315377242415E-4
28-29	0.0	0.0	0.0	0.004324076989292148	1.0572315377242415E-4
30-31	0.0	0.0	0.0	0.013056809490894382	1.1629546914966655E-4
32-33	0.0	0.0	0.0	0.03413800635311576	1.2686778452690898E-4
34-35	0.0	0.0	0.0	0.06823372344472255	1.2686778452690898E-4
36-37	0.0	0.0	0.0	0.1224379843838444	1.2686778452690898E-4
38-39	0.0	0.0	0.0	0.19780802070820558	1.2686778452690898E-4
40-41	0.0	0.0	0.0	0.2773224046604458	1.2686778452690898E-4
42-43	0.0	0.0	0.0	0.3603785142640622	1.480124152813938E-4
44-45	0.0	0.0	0.0	0.46056177477881133	1.6915704603587863E-4
46-47	0.0	0.0	0.0	0.5746053407531253	1.7972936141312106E-4
48-49	0.0	0.0	0.0	0.6953411823612337	2.114463075448483E-4
50-51	0.0	0.0	0.0	0.8163307595383957	2.5373556905381795E-4
52-53	0.0	1.0572315377242415E-5	0.0	0.9346984025020019	2.748801998083028E-4
54-55	0.0	2.114463075448483E-5	0.0	1.0622005259515452	2.748801998083028E-4
56-57	0.0	2.114463075448483E-5	0.0	1.214262138022423	2.748801998083028E-4
58-59	0.0	2.114463075448483E-5	0.0	1.3798034521992848	2.748801998083028E-4
60-61	0.0	2.114463075448483E-5	0.0	1.5449430183918114	2.748801998083028E-4
62-63	0.0	2.114463075448483E-5	0.0	1.7113935516911158	3.171694613172724E-4
64-65	0.0	2.114463075448483E-5	0.0	1.8833205443558318	3.171694613172724E-4
66-67	0.0	2.114463075448483E-5	0.0	2.076106715259847	3.171694613172724E-4
68-69	0.0	2.114463075448483E-5	0.0	2.2872887149202645	3.171694613172724E-4
70-71	0.0	2.114463075448483E-5	0.0	2.511654391856103	3.2774177669451485E-4
72-73	0.0	2.114463075448483E-5	0.0	2.7347513909466725	3.594587228262421E-4
74-75	0.0	2.114463075448483E-5	0.0	2.959243935667038	3.806033535807269E-4
76-77	0.0	2.114463075448483E-5	0.0	3.207048435794223	3.806033535807269E-4
78-79	0.0	2.114463075448483E-5	0.0	3.4754160893301442	3.806033535807269E-4
80-81	0.0	2.114463075448483E-5	0.0	3.754017744151237	4.0174798433521174E-4
82-83	0.0	2.114463075448483E-5	0.0	4.037228928476806	4.4403724584418143E-4
84-85	0.0	2.114463075448483E-5	0.0	4.325567685760339	4.4403724584418143E-4
86-87	0.0	3.1716946131727244E-5	0.0	4.630187808724824	4.4403724584418143E-4
88	0.0	4.228926150896966E-5	0.0	4.8714057563719875	4.4403724584418143E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7840	0.0	53.655464	1
GTATCAA	14430	0.0	44.738384	1
ATCAACG	17345	0.0	36.40712	3
CAACGCA	17415	0.0	36.287395	5
TATCAAC	18345	0.0	35.03392	2
AACGCAG	19345	0.0	33.259968	6
TCAACGC	19285	0.0	32.841877	4
ACGCAGA	20655	0.0	30.58005	7
CGCAGAG	20865	0.0	30.272272	8
GCAGAGT	23755	0.0	26.332201	9
GTGGTAT	2245	0.0	25.989494	1
GTACTAG	1985	0.0	25.12684	1
TACATGG	13495	0.0	24.405117	2
GTACATG	14065	0.0	24.053686	1
ACATGGG	13915	0.0	23.248226	3
GAGTACT	16170	0.0	22.757046	12-13
CAGAGTA	22815	0.0	22.49398	10-11
CTAACGC	1020	0.0	22.12716	3
AGAGTAC	21920	0.0	22.04026	10-11
TAACGCC	1030	0.0	21.912102	4
>>END_MODULE