FastQCFastQC Report
Mon 16 Jan 2017
ERR1146528_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146528_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3650915
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120620.330382931402128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC5550.026.2496974
CTAACGC6150.025.2170583
GTACTAG14750.024.5493471
TCTAACG6650.021.9076422
CGCCTAC7000.021.4806797
GCATCGT13050.020.5253318
TATTCGC2151.1973552E-619.6711569
CATGGGG67750.019.0062684
ACGCCTA7600.018.5500626
GTATAAG17400.017.837581
TACCTGG34850.017.5305752
TAGTACT21750.017.2856754
CGTTAAC3551.8135324E-917.2209151
CTAGTAC20600.016.8818533
GTATTAG9500.016.3354681
TGATCGC8100.016.2442199
CTCGTCG11400.016.0762839
GTACCTG38050.016.066821
GTATTAT12650.015.9852391
GACTCGT11500.015.9353957
Produced by FastQC (version 0.11.5)