##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146528_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3650915
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.105586133886984	31.0	31.0	34.0	28.0	34.0
2	31.364424534671446	31.0	31.0	34.0	28.0	34.0
3	31.5768189618219	33.0	31.0	34.0	28.0	34.0
4	35.166249556618	37.0	35.0	37.0	32.0	37.0
5	35.22524599997535	37.0	35.0	37.0	32.0	37.0
6	35.26190365976748	37.0	35.0	37.0	32.0	37.0
7	35.17626266292149	37.0	35.0	37.0	32.0	37.0
8	35.16001495515508	37.0	35.0	37.0	32.0	37.0
9	36.87816834957812	39.0	37.0	39.0	33.0	39.0
10-11	36.850121270969055	39.0	37.0	39.0	33.0	39.0
12-13	36.81567155083041	39.0	37.0	39.0	33.0	39.0
14-15	38.197657025704515	40.0	38.0	41.0	33.0	41.0
16-17	38.20554641781581	40.0	38.0	41.0	33.0	41.0
18-19	38.22193039279195	40.0	38.0	41.0	33.0	41.0
20-21	38.29693200197758	40.0	38.5	41.0	33.0	41.0
22-23	38.3006108057843	40.0	38.5	41.0	33.0	41.0
24-25	38.338479942699294	40.0	38.0	41.0	33.0	41.0
26-27	38.34625032354903	40.0	38.0	41.0	33.0	41.0
28-29	38.29424500433453	40.0	38.0	41.0	33.0	41.0
30-31	38.28466096855172	40.0	38.0	41.0	33.0	41.0
32-33	38.27877600546712	40.0	38.0	41.0	33.0	41.0
34-35	38.21775705542309	40.0	38.0	41.0	33.0	41.0
36-37	38.124480575417394	40.0	38.0	41.0	33.0	41.0
38-39	38.07760150537605	40.0	38.0	41.0	33.0	41.0
40-41	38.02177248717102	40.0	38.0	41.0	32.5	41.0
42-43	37.90221930666696	40.0	38.0	41.0	32.0	41.0
44-45	37.760976084077555	40.0	37.5	41.0	32.0	41.0
46-47	37.63559244737278	40.0	37.0	41.0	32.0	41.0
48-49	37.5208120430084	40.0	37.0	41.0	31.0	41.0
50-51	37.39448302685766	40.0	37.0	41.0	31.0	41.0
52-53	37.28485160569337	40.0	36.0	41.0	31.0	41.0
54-55	37.10949802994591	39.5	36.0	41.0	31.0	41.0
56-57	36.96259554111777	39.0	35.5	41.0	30.0	41.0
58-59	36.743689732573884	39.0	35.0	41.0	30.0	41.0
60-61	36.54819148076578	39.0	35.0	41.0	30.0	41.0
62-63	36.306842257351924	38.0	35.0	40.0	30.0	41.0
64-65	36.063964513005644	38.0	35.0	40.0	30.0	41.0
66-67	35.78925022905217	37.0	35.0	40.0	29.5	41.0
68-69	35.48334280584456	37.0	34.5	39.5	29.0	41.0
70-71	35.17956758785127	36.0	34.0	39.0	29.0	41.0
72-73	34.845652391249864	36.0	34.0	39.0	29.0	40.5
74-75	34.521709900120925	35.0	34.0	38.5	29.0	40.0
76-77	34.189338700024514	35.0	34.0	37.0	28.0	39.0
78-79	33.90299459176672	35.0	34.0	37.0	28.0	39.0
80-81	33.61384680278779	35.0	34.0	36.0	28.0	39.0
82-83	33.36172726563068	35.0	33.5	36.0	27.5	37.0
84-85	33.141319093980556	35.0	33.0	36.0	27.0	37.0
86-87	32.945318365396076	35.0	33.0	35.0	27.0	37.0
88-89	32.78283539879729	35.0	33.0	35.0	27.0	36.0
90-91	32.61499665152434	35.0	33.0	35.0	26.5	36.0
92-93	32.510077747633126	35.0	33.0	35.0	26.0	36.0
94-95	32.42386867401734	35.0	33.0	35.0	26.0	36.0
96-97	32.34816915759474	35.0	33.0	35.0	26.0	35.0
98-99	32.28751847139689	35.0	33.0	35.0	25.0	35.0
100	31.294831843524157	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11511722755814446
1101	2	0.09486616376440793
1101	3	0.23275396839710183
1101	4	0.19805388815283465
1101	5	0.23362661832156562
1101	6	0.23181295970399418
1101	7	0.3062353311884607
1101	8	0.33780067635912303
1101	9	0.4511498322527956
1101	10-11	0.4857728157711634
1101	12-13	0.5420773865893764
1101	14-15	0.6112224729049487
1101	16-17	0.5708812779631245
1101	18-19	0.5263862225945388
1101	20-21	0.5266154520168627
1101	22-23	0.5237847062437808
1101	24-25	0.4684729885287453
1101	26-27	0.47798527788572187
1101	28-29	0.4953384750262657
1101	30-31	0.4962423447062889
1101	32-33	0.4746949210590756
1101	34-35	0.47192084391612354
1101	36-37	0.480123646224186
1101	38-39	0.46682907156579034
1101	40-41	0.39711247964542906
1101	42-43	0.4371444200589849
1101	44-45	0.34320133979903744
1101	46-47	0.3118617533690653
1101	48-49	0.2651756304650732
1101	50-51	0.3724523974372502
1101	52-53	0.3097644383329694
1101	54-55	0.3983286925549976
1101	56-57	0.38768082602727816
1101	58-59	0.40797387670859564
1101	60-61	0.3511213095546779
1101	62-63	0.3573486435246451
1101	64-65	0.3122553479654684
1101	66-67	0.2802604128348136
1101	68-69	0.3183934169072842
1101	70-71	0.34725649520411395
1101	72-73	0.31906723345291255
1101	74-75	0.3344607995381068
1101	76-77	0.3021355891215265
1101	78-79	0.2641989179675832
1101	80-81	0.3572813958613352
1101	82-83	0.34673154008489604
1101	84-85	0.3348574227005727
1101	86-87	0.30668754824419153
1101	88-89	0.35701941791163705
1101	90-91	0.34769068375995715
1101	92-93	0.2846954051114281
1101	94-95	0.2705802237318551
1101	96-97	0.23887315872870118
1101	98-99	0.2671221615637478
1101	100	0.2375315563162843
1102	1	0.02452851361126207
1102	2	0.02903978128669138
1102	3	0.10805518665112146
1102	4	0.049564846612447866
1102	5	0.02734622601303016
1102	6	0.06484507310900511
1102	7	0.0679354419936189
1102	8	0.06977406302895162
1102	9	0.2849272233789364
1102	10-11	0.2247600586112668
1102	12-13	0.1613925644854035
1102	14-15	0.3281916152247888
1102	16-17	0.2749859109850732
1102	18-19	0.2396255808287293
1102	20-21	0.18177835971305
1102	22-23	0.1942787887659989
1102	24-25	0.23030568378642613
1102	26-27	0.22699119586579286
1102	28-29	0.2405308470503229
1102	30-31	0.24422996024553356
1102	32-33	0.19735398372576896
1102	34-35	0.23488495730907744
1102	36-37	0.2367639292746162
1102	38-39	0.20892303795857714
1102	40-41	0.19738526326595718
1102	42-43	0.16519046196403053
1102	44-45	0.1556460150736143
1102	46-47	0.13699258482969867
1102	48-49	0.11086902665831389
1102	50-51	0.16234666540491816
1102	52-53	0.1428105354899074
1102	54-55	0.09240625214918907
1102	56-57	0.08741778683157975
1102	58-59	0.08410188429517973
1102	60-61	0.0618951273901871
1102	62-63	0.044975434062706654
1102	64-65	0.024030671666224634
1102	66-67	0.06236247614142343
1102	68-69	-0.026563943699727588
1102	70-71	-0.018771888195800557
1102	72-73	-0.03312167578430092
1102	74-75	7.972273123257878E-4
1102	76-77	0.011544564640921351
1102	78-79	0.03830249618065551
1102	80-81	-0.05989995391227865
1102	82-83	-0.014037204622226795
1102	84-85	0.03311578256318626
1102	86-87	0.014836800686076401
1102	88-89	-0.05110614653667511
1102	90-91	0.07410675462703864
1102	92-93	0.003535798464717743
1102	94-95	0.0335351552647154
1102	96-97	0.011095178918751003
1102	98-99	-0.06001377115350692
1102	100	-0.1640048481516878
1103	1	0.05007949142822099
1103	2	0.06642051520413617
1103	3	0.16862646443989604
1103	4	0.03173565423348634
1103	5	0.14759042537853873
1103	6	0.1774586420059734
1103	7	0.1481302257704371
1103	8	0.157353598738311
1103	9	0.2884522819999802
1103	10-11	0.32210816864490965
1103	12-13	0.29062932152741894
1103	14-15	0.38774459605225076
1103	16-17	0.35330542114705565
1103	18-19	0.3139300744089013
1103	20-21	0.3151284847512841
1103	22-23	0.3108665943900206
1103	24-25	0.292262876933016
1103	26-27	0.3044303409281284
1103	28-29	0.31445632786279987
1103	30-31	0.31094400350816187
1103	32-33	0.33719230359574937
1103	34-35	0.3240758969848798
1103	36-37	0.3073316688112868
1103	38-39	0.33042901400640545
1103	40-41	0.28934563484239817
1103	42-43	0.2868865440994597
1103	44-45	0.24181564727530258
1103	46-47	0.19851919068639745
1103	48-49	0.19773131668704025
1103	50-51	0.20535621868560128
1103	52-53	0.15712906213774147
1103	54-55	0.2382206198515462
1103	56-57	0.23491852146867132
1103	58-59	0.23187908235934174
1103	60-61	0.1718670012844825
1103	62-63	0.20191250714859876
1103	64-65	0.24048594276229807
1103	66-67	0.22705251926011982
1103	68-69	0.22382136595730628
1103	70-71	0.23132316484799276
1103	72-73	0.2080060071403409
1103	74-75	0.11368186230264143
1103	76-77	0.1918125122747938
1103	78-79	0.18446349264840478
1103	80-81	0.21078472785492863
1103	82-83	0.20967867973090648
1103	84-85	0.2411973902489919
1103	86-87	0.24216050277724577
1103	88-89	0.18867400206990936
1103	90-91	0.18592271754135936
1103	92-93	0.19282269073993774
1103	94-95	0.138220040931202
1103	96-97	0.17005625214857645
1103	98-99	0.18317269473234177
1103	100	0.11380071589967145
1104	1	0.022921163132632927
1104	2	0.0347307935564416
1104	3	0.1070667638756504
1104	4	0.09273305536902399
1104	5	0.10688348382064561
1104	6	0.14839318209928365
1104	7	0.11324597154479932
1104	8	0.1584646276635837
1104	9	0.1908776478366363
1104	10-11	0.22092074396776695
1104	12-13	0.23306665189168285
1104	14-15	0.2820189930569299
1104	16-17	0.2415836878069726
1104	18-19	0.19852712621137414
1104	20-21	0.23152324297569038
1104	22-23	0.21626926266134916
1104	24-25	0.1822716621664071
1104	26-27	0.2537962524799724
1104	28-29	0.21764292610883018
1104	30-31	0.1985233259718342
1104	32-33	0.21494224941798024
1104	34-35	0.24789880269665332
1104	36-37	0.22235050636121656
1104	38-39	0.3239174857743663
1104	40-41	0.1989758560452657
1104	42-43	0.22192685254475464
1104	44-45	0.16230405731119646
1104	46-47	0.1530876325486048
1104	48-49	0.13943553702862488
1104	50-51	0.15585788906638953
1104	52-53	0.049513086026756525
1104	54-55	0.1284018841131811
1104	56-57	0.11007299531031123
1104	58-59	0.11555145080746598
1104	60-61	0.1126599784367599
1104	62-63	0.12714169903874506
1104	64-65	0.047941637953449856
1104	66-67	0.031079266856977483
1104	68-69	0.014945721964842562
1104	70-71	-0.031083655414732902
1104	72-73	-0.002240125846114438
1104	74-75	0.03224801358995677
1104	76-77	0.024420632841049894
1104	78-79	0.07724087772618304
1104	80-81	0.0632302724772984
1104	82-83	0.10089242673954146
1104	84-85	0.06348116533737169
1104	86-87	0.03384832611748578
1104	88-89	-0.03286470588346191
1104	90-91	0.04683606666549878
1104	92-93	-0.039585458708764065
1104	94-95	-0.026019906613228727
1104	96-97	-0.04737402908085642
1104	98-99	-0.11137178470596609
1104	100	-0.07534074304229321
1105	1	0.00900444351883678
1105	2	0.027949418231123957
1105	3	0.05532836590396073
1105	4	0.07690081044292896
1105	5	0.0958736954770103
1105	6	0.04206313416650431
1105	7	0.06143660834964493
1105	8	0.08515926612844993
1105	9	0.09802145652268024
1105	10-11	0.16118841893347025
1105	12-13	0.1576756735109015
1105	14-15	0.19666761519943776
1105	16-17	0.1163102645510179
1105	18-19	0.09460202077247715
1105	20-21	0.18306951340854738
1105	22-23	0.17410256260138368
1105	24-25	0.18689288394683246
1105	26-27	0.12644905182054345
1105	28-29	0.15288462394556035
1105	30-31	0.20348449867304197
1105	32-33	0.21434159862366897
1105	34-35	0.21440289578644922
1105	36-37	0.11549145143798967
1105	38-39	0.167365077760401
1105	40-41	0.13315911132399805
1105	42-43	0.13057134177550722
1105	44-45	0.10944050161420904
1105	46-47	0.11462944577971257
1105	48-49	0.10822112455246469
1105	50-51	0.09999147226016447
1105	52-53	0.07069611594535985
1105	54-55	0.10011843893908434
1105	56-57	0.0047157280892804465
1105	58-59	0.05632943608798513
1105	60-61	0.05153906362467353
1105	62-63	0.0835390514323322
1105	64-65	0.09067868483585784
1105	66-67	0.058096695301564694
1105	68-69	0.029689506079137118
1105	70-71	0.09091829603962509
1105	72-73	0.20287128098319585
1105	74-75	0.1636763396071217
1105	76-77	0.14816941094989744
1105	78-79	0.08293122077714088
1105	80-81	0.0917399884985457
1105	82-83	0.1429454645832564
1105	84-85	0.17109256948807428
1105	86-87	0.129864773477685
1105	88-89	0.033022075477575186
1105	90-91	0.09783627631112068
1105	92-93	0.0734131773446407
1105	94-95	0.10870233793071549
1105	96-97	0.08030747956765794
1105	98-99	0.06926599515040976
1105	100	0.05025823808710328
1106	1	0.09897281002425729
1106	2	0.10910841039045138
1106	3	0.17852174871848092
1106	4	0.17752732595810983
1106	5	0.14897673204631445
1106	6	0.1525397290731405
1106	7	0.11786670078837602
1106	8	0.20570257447501916
1106	9	0.25397212304164185
1106	10-11	0.25801429931701136
1106	12-13	0.26215636723220115
1106	14-15	0.26292040005474604
1106	16-17	0.25578935813447146
1106	18-19	0.20845890690421243
1106	20-21	0.22700899127125496
1106	22-23	0.22920515605559189
1106	24-25	0.24189300703435634
1106	26-27	0.22651551908541023
1106	28-29	0.25540545650959245
1106	30-31	0.2086329422312616
1106	32-33	0.23060976941992095
1106	34-35	0.26709266598203385
1106	36-37	0.2807075315925829
1106	38-39	0.29366457433241067
1106	40-41	0.2754276760003407
1106	42-43	0.17646975763902617
1106	44-45	0.15705229586109937
1106	46-47	0.1537743567258829
1106	48-49	0.16427456915692318
1106	50-51	0.16890079367862398
1106	52-53	0.17369483583507161
1106	54-55	0.22955697518220575
1106	56-57	0.15342484764419595
1106	58-59	0.20595709631717796
1106	60-61	0.15579019910711622
1106	62-63	0.1505962132801244
1106	64-65	0.16988673968365475
1106	66-67	0.11496559477598822
1106	68-69	0.14905302047421998
1106	70-71	0.13921230290790731
1106	72-73	0.12227541426236854
1106	74-75	0.07129361124898992
1106	76-77	0.14794323761804407
1106	78-79	0.07234723200272697
1106	80-81	0.18496155835686778
1106	82-83	0.13952116956706817
1106	84-85	0.1912333816195826
1106	86-87	0.1437259676728786
1106	88-89	0.06740134507618478
1106	90-91	0.07591961887602139
1106	92-93	0.12632235105807155
1106	94-95	0.062476201525115016
1106	96-97	0.033760392433990205
1106	98-99	0.06695533453965652
1106	100	0.08855692228143042
1107	1	0.03680164828752197
1107	2	0.015216179008490371
1107	3	0.1044334428584257
1107	4	0.09930725589818934
1107	5	0.16237961532709022
1107	6	0.14196482026528656
1107	7	0.080865342048142
1107	8	0.10896240458702522
1107	9	0.1878429434922424
1107	10-11	0.21448880327008624
1107	12-13	0.18362690407493432
1107	14-15	0.22863993148110495
1107	16-17	0.24563922860545517
1107	18-19	0.16538961866349666
1107	20-21	0.16166122572095531
1107	22-23	0.17864950364482723
1107	24-25	0.17833706287198225
1107	26-27	0.16731395785848946
1107	28-29	0.1638279658185624
1107	30-31	0.19320879942951308
1107	32-33	0.18024461539444303
1107	34-35	0.13109821598324345
1107	36-37	0.2257768898062409
1107	38-39	0.2475478555022974
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2216	38-39	-0.5045177000188374
2216	40-41	-0.5366027227272667
2216	42-43	-0.44911147449201394
2216	44-45	-0.4236118205461352
2216	46-47	-0.38069910484450276
2216	48-49	-0.339724322543816
2216	50-51	-0.40226841474548536
2216	52-53	-0.38014623132580994
2216	54-55	-0.3294112710642736
2216	56-57	-0.3694850195411874
2216	58-59	-0.37244553274614134
2216	60-61	-0.3708812359940339
2216	62-63	-0.36905863010137807
2216	64-65	-0.43038706383567416
2216	66-67	-0.4897915450101422
2216	68-69	-0.37243072219900597
2216	70-71	-0.48498387889866734
2216	72-73	-0.4538899243482817
2216	74-75	-0.4860093004970878
2216	76-77	-0.4377286640228206
2216	78-79	-0.4533455169701881
2216	80-81	-0.43509491400608624
2216	82-83	-0.4567334444521194
2216	84-85	-0.46185957509469944
2216	86-87	-0.4386218807547593
2216	88-89	-0.41562692162236914
2216	90-91	-0.4019476578394574
2216	92-93	-0.37396052312834627
2216	94-95	-0.4251865933011203
2216	96-97	-0.4404379373921259
2216	98-99	-0.4063873346553173
2216	100	-0.42244516065002813
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	84.0
1	56.0
2	38.0
3	57.0
4	32.0
5	38.0
6	102.0
7	469.0
8	1438.0
9	2074.0
10	2261.0
11	3136.0
12	4527.0
13	5965.0
14	7272.0
15	8111.0
16	8790.0
17	9563.0
18	10680.0
19	11827.0
20	13087.0
21	14666.0
22	16371.0
23	19357.0
24	23807.0
25	29151.0
26	33155.0
27	32165.0
28	34412.0
29	43072.0
30	50412.0
31	57982.0
32	74036.0
33	99633.0
34	149024.0
35	232553.0
36	434720.0
37	873465.0
38	1044056.0
39	298574.0
40	697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57290049964906	19.228143471167336	12.876919041833654	24.32203698734995
2	14.824962348510622	21.0121333944742	39.78900921660248	24.37389504041269
3	19.06370813697933	25.920974812539736	29.747265242882705	25.26805180759823
4	12.272053282506693	17.367135459881844	39.67154251421487	30.689268743396596
5	13.259690453362552	38.28001643610464	35.34066566223805	13.119627448294754
6	29.91659731509428	37.70641794699988	18.77154852427707	13.605436213628774
7	26.447475786953127	32.48562446638715	23.173006838735724	17.893892907924
8	25.12505704332334	34.61473774862808	21.30015498253228	18.96005022551629
9	26.00806208617163	16.249190559097677	20.956493441748194	36.7862539129825
10-11	24.192774780623363	26.24886836306063	28.858323848811935	20.70003300750407
12-13	25.662611923618016	24.00011066358708	28.058273144029204	22.279004268765696
14-15	22.965924650428022	25.408240874065886	26.111747160684295	25.514087314821804
16-17	22.325933106913094	28.133862873814934	26.9965250789154	22.54367894035657
18-19	22.09091977394692	28.669397506798113	27.613077900985772	21.6266048182692
20-21	23.35284291929951	27.467195188627663	26.799251866225614	22.38071002584721
22-23	22.87508710343286	27.44347041989333	26.376042502092673	23.305399974581135
24-25	23.136822602650987	27.501011424093342	26.535700599842283	22.82646537341339
26-27	23.20627487932266	27.16781974102564	26.814248314899892	22.81165706475181
28-29	22.49060696026538	28.021053995409385	26.91555625435446	22.57278278997077
30-31	23.09278025757304	27.53251164529812	26.691845072679538	22.682863024449304
32-33	22.729668994937356	27.678850128206236	26.551681678732614	23.0397991981238
34-35	23.095852037438252	27.565401476130198	26.85408620374629	22.48466028268526
36-37	23.311713928010803	27.12607560536618	27.025535097403754	22.536675369219264
38-39	23.179456454560995	27.31841273598345	27.06440315418973	22.43772765526582
40-41	22.895814016816036	27.343038472133053	27.115109846119413	22.646037664931495
42-43	22.843748060804405	27.354654336544765	26.587277397469034	23.21432020518179
44-45	23.171468447320144	27.326038878916002	26.56925019233425	22.93324248142961
46-47	23.10394130989397	27.307949486199917	26.780059644127117	22.808049559778997
48-49	23.25023378255934	27.11755762503771	26.96419133427714	22.66801725812581
50-51	22.63422146910581	27.429365539608686	27.140521740893526	22.795891250391982
52-53	22.702826071738226	27.599458981608088	26.544916979322196	23.152797967331487
54-55	22.549084616933797	27.27627813177735	26.762614174566007	23.412023076722843
56-57	23.298900879690827	26.981825276259748	26.499480471652824	23.219793372396605
58-59	23.28631772308394	26.938270502152584	26.833299174039226	22.942112600724247
60-61	23.01036557138056	27.31157283526785	27.066157907579147	22.611903685772443
62-63	22.879024179601107	27.12129091582623	26.848473074958733	23.15121182961393
64-65	22.38243877631823	27.611505780482236	26.858836110936817	23.14721933226272
66-67	23.055756037332902	27.01734902626138	26.577595216057247	23.34929972034847
68-69	22.78116695230359	27.08957372028882	26.70200809903639	23.427251228371208
70-71	22.96762525860061	27.054473961829867	26.454438340023838	23.523462439545682
72-73	23.149434691627935	26.671888242029745	27.009705992518086	23.168971073824235
74-75	23.03967975825683	27.48237905922823	26.549071336758285	22.928869845756658
76-77	22.974712375968025	26.874603114799577	26.720390737118997	23.430293772113398
78-79	23.047984781654137	26.917209485846517	26.899796344336313	23.135009388163034
80-81	22.86273287457741	27.21536980774039	26.846456059106217	23.075441258575985
82-83	22.928768187045538	26.75558643948347	27.21645690774124	23.099188465729757
84-85	23.017940514480244	27.096502138769957	26.615071687119112	23.270485659630687
86-87	22.79881304987547	27.158525792601562	26.810126339497327	23.23253481802564
88-89	23.156559394919945	26.59947969252815	26.728981180980142	23.51497973157176
90-91	23.12295222558384	26.530224177698187	26.84263393040622	23.50418966631175
92-93	23.153930082545628	26.94714898929148	26.893198137448906	23.005722790713985
94-95	22.71083022593951	26.993258929421593	27.07280419247104	23.223106652167854
96-97	22.670780266502636	26.83411502233413	27.12711311189398	23.367991599269253
98-99	22.937010380911047	27.103745544932075	26.477898917632203	23.481345156524675
100	22.856305250482553	26.592344079422332	26.517409595723667	24.033941074371448
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1137.0
1	806.0
2	458.0
3	426.0
4	384.5
5	327.5
6	271.0
7	258.0
8	274.5
9	290.5
10	277.5
11	253.5
12	247.5
13	286.5
14	343.0
15	385.5
16	454.0
17	557.5
18	714.0
19	992.5
20	1506.0
21	2118.5
22	3055.0
23	4613.0
24	6869.5
25	10147.5
26	14103.0
27	18933.5
28	24268.0
29	30076.0
30	36683.0
31	43906.5
32	52214.0
33	60325.5
34	68238.0
35	76351.5
36	85275.0
37	94803.5
38	101173.5
39	104710.5
40	107216.5
41	109128.0
42	110560.5
43	114733.0
44	122013.5
45	130716.5
46	140324.0
47	156590.5
48	189488.5
49	191955.0
50	174370.5
51	163633.0
52	143957.0
53	131474.0
54	122889.5
55	112191.0
56	101629.0
57	89415.5
58	75653.0
59	67112.5
60	57785.5
61	45949.5
62	35642.0
63	27410.5
64	20417.0
65	14433.0
66	10461.0
67	8042.5
68	6163.5
69	4602.5
70	3662.0
71	2886.5
72	2231.5
73	1892.5
74	1537.5
75	1234.5
76	1053.5
77	820.5
78	587.0
79	379.0
80	218.0
81	131.0
82	93.0
83	51.0
84	38.0
85	28.5
86	25.0
87	22.5
88	16.5
89	16.5
90	13.5
91	8.5
92	6.5
93	8.0
94	9.5
95	5.0
96	5.0
97	7.0
98	4.5
99	5.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05899890849280249
2	0.009011439598018579
3	0.011175280717299636
4	0.00920317235542323
5	0.011367013474704287
6	0.011860040565173388
7	0.002574697028005308
8	0.004957661298605966
9	0.006765427296992672
10-11	0.00629979060043852
12-13	0.005779373116054469
14-15	0.009408600309785355
16-17	0.008997744401061105
18-19	0.007340625569206623
20-21	0.006327180994353469
22-23	0.0022734026949408574
24-25	0.003341628057623911
26-27	0.004587890980754139
28-29	0.005656116343437194
30-31	0.008217118174485026
32-33	0.009627723461104956
34-35	0.011613527019938837
36-37	0.017666804075142806
38-39	0.01805026958995211
40-41	0.01779006084776008
42-43	0.024199413023858404
44-45	0.027910811399334136
46-47	0.030143128503402575
48-49	0.0316632953656823
50-51	0.03263565434966303
52-53	0.030992230714766023
54-55	0.03697703178518262
56-57	0.039743461570592574
58-59	0.036894860603437774
60-61	0.037839829193503545
62-63	0.03360801333364376
64-65	0.035771854452924814
66-67	0.03760701084522647
68-69	0.040222793464104206
70-71	0.04012692708540188
72-73	0.03599097760424442
74-75	0.038949140147059025
76-77	0.0384150274657175
78-79	0.037593315648268996
80-81	0.04252358655296001
82-83	0.038565674632249726
84-85	0.034799495468944085
86-87	0.033553232545813855
88-89	0.0314989530021926
90-91	0.033443670970154056
92-93	0.035525340907690266
94-95	0.0389080545561866
96-97	0.03375866050017598
98-99	0.03119765866912815
100	0.02903381754984709
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3650915.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.3044653207465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95575306880282	46.077736523449175
2	14.149395841205623	17.63141084995821
3	5.478938779850942	10.240870536111487
4	2.486339985902616	6.196403337090197
5	1.314425190391331	4.0947279345726155
6	0.8088458243633809	3.0236823968327338
7	0.5010175969233098	2.1850943444814477
8	0.32847151256718116	1.6372193570876072
9	0.23467957586874172	1.3159426946581365
>10	0.7363172695197878	6.656847687395059
>50	0.004250435350470183	0.17284144916373642
>100	0.0013613647883080596	0.12388943987077024
>500	0.0	0.0
>1k	1.3570297698834764E-4	0.1334183444658687
>5k	0.0	0.0
>10k+	6.785148849417382E-5	0.5099151048628987
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	12062	0.330382931402128	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.4780787829900175E-5
7	0.0	0.0	0.0	0.0	5.4780787829900175E-5
8	0.0	0.0	0.0	0.0	5.4780787829900175E-5
9	0.0	0.0	0.0	0.0	5.4780787829900175E-5
10-11	0.0	0.0	0.0	0.0	6.847598478737522E-5
12-13	0.0	0.0	0.0	2.7390393914950087E-5	4.656366965541515E-4
14-15	0.0	0.0	0.0	2.7390393914950087E-5	6.710646509162771E-4
16-17	0.0	0.0	0.0	4.108559087242513E-5	8.764926052784028E-4
18-19	0.0	0.0	0.0	5.4780787829900175E-5	9.17578196150828E-4
20-21	0.0	0.0	0.0	8.217118174485026E-5	0.0010408349687681033
22-23	1.3695196957475044E-5	0.0	0.0	1.7803756044717557E-4	0.0010819205596405285
24-25	2.7390393914950087E-5	0.0	0.0	5.067222874265766E-4	0.0010956157565980035
26-27	2.7390393914950087E-5	0.0	0.0	0.0014379956805348796	0.0011777869383428538
28-29	2.7390393914950087E-5	0.0	0.0	0.0042181206629023135	0.0012599581200877041
30-31	2.7390393914950087E-5	0.0	0.0	0.013106303488303618	0.001287348514002654
32-33	2.7390393914950087E-5	0.0	0.0	0.033594318136686285	0.0013695196957475043
34-35	2.7390393914950087E-5	0.0	0.0	0.06635322925896658	0.0014516908774923547
36-37	2.7390393914950087E-5	0.0	0.0	0.11546420554847209	0.0014516908774923547
38-39	2.7390393914950087E-5	0.0	0.0	0.1836115056088679	0.0014790812714073048
40-41	2.7390393914950087E-5	0.0	0.0	0.2567164669678697	0.0014790812714073048
42-43	2.7390393914950087E-5	0.0	0.0	0.3287258125702735	0.0014790812714073048
44-45	2.7390393914950087E-5	0.0	0.0	0.4144988311149397	0.00157494765010963
46-47	2.7390393914950087E-5	0.0	0.0	0.517199113099045	0.0016845092257694303
48-49	2.7390393914950087E-5	0.0	0.0	0.6224603969141982	0.0016982044227269054
50-51	2.7390393914950087E-5	0.0	0.0	0.7249963365348139	0.0017255948166418555
52-53	2.7390393914950087E-5	0.0	0.0	0.8284909399424528	0.0019173275740465062
54-55	2.7390393914950087E-5	0.0	0.0	0.9407230790089607	0.0019447179679614563
56-57	2.7390393914950087E-5	0.0	0.0	1.0720874082250613	0.0020405843466637817
58-59	2.7390393914950087E-5	0.0	0.0	1.2147639701280366	0.0021090603314511567
60-61	2.7390393914950087E-5	0.0	0.0	1.3584813669997795	0.002136450725366107
62-63	2.7390393914950087E-5	0.0	0.0	1.5005279498427107	0.0023281834827707576
64-65	2.7390393914950087E-5	0.0	0.0	1.6506683940875095	0.0023281834827707576
66-67	4.108559087242513E-5	0.0	0.0	1.8168596091664693	0.0023281834827707576
68-69	5.4780787829900175E-5	0.0	0.0	2.0006765427296993	0.0023281834827707576
70-71	5.4780787829900175E-5	0.0	0.0	2.1994075457796196	0.0023281834827707576
72-73	5.4780787829900175E-5	0.0	0.0	2.391619635077782	0.002519916240175408
74-75	5.4780787829900175E-5	0.0	0.0	2.5843932274512005	0.0026979538006225833
76-77	5.4780787829900175E-5	0.0	0.0	2.791108530327329	0.002725344194537534
78-79	5.4780787829900175E-5	0.0	0.0	3.019407463608438	0.002766429785409959
80-81	5.4780787829900175E-5	0.0	0.0	3.2614700698318093	0.002862296164112284
82-83	5.4780787829900175E-5	0.0	0.0	3.5069427800975923	0.0031772856941342104
84-85	5.4780787829900175E-5	0.0	0.0	3.7615775771279254	0.0031772856941342104
86-87	5.4780787829900175E-5	0.0	0.0	4.022471079167825	0.0032457616789215854
88	5.4780787829900175E-5	0.0	0.0	4.22855640298391	0.0033142376637089604
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	555	0.0	26.249697	4
CTAACGC	615	0.0	25.217058	3
GTACTAG	1475	0.0	24.549347	1
TCTAACG	665	0.0	21.907642	2
CGCCTAC	700	0.0	21.480679	7
GCATCGT	1305	0.0	20.525331	8
TATTCGC	215	1.1973552E-6	19.671156	9
CATGGGG	6775	0.0	19.006268	4
ACGCCTA	760	0.0	18.550062	6
GTATAAG	1740	0.0	17.83758	1
TACCTGG	3485	0.0	17.530575	2
TAGTACT	2175	0.0	17.285675	4
CGTTAAC	355	1.8135324E-9	17.220915	1
CTAGTAC	2060	0.0	16.881853	3
GTATTAG	950	0.0	16.335468	1
TGATCGC	810	0.0	16.244219	9
CTCGTCG	1140	0.0	16.076283	9
GTACCTG	3805	0.0	16.06682	1
GTATTAT	1265	0.0	15.985239	1
GACTCGT	1150	0.0	15.935395	7
>>END_MODULE