FastQCFastQC Report
Mon 16 Jan 2017
ERR1146528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3650915
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT128440.35180221944361895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89460.2450344639631435No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA87230.23892640612010962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76530.20961868463111305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.11101326653729271No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36720.10057752645569673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13500.023.7078271
TACGGGC8950.021.5392674
GAGTACT101750.021.29258512-13
ATACGGG9500.020.787193
ACGGGCG9650.019.9768315
CATGGGG63650.019.6495824
GGCGTCG10100.019.5429348
CGGGCGT10150.018.9844266
GTACTTT113900.018.91809814-15
AGTACTT108700.018.80708912-13
GCATCGT14950.018.5469578
CTAGTAC21050.017.645953
GACTCGT9750.017.3526467
TAGTACT21050.016.7524854
TAGGCAT22200.016.731865
CTCGTCG10200.016.5868599
TATCACG2002.964367E-416.4718722
TACCTGG34200.016.3755442
GTATTAG9050.016.12241
ACTTTTT134000.016.09802816-17
Produced by FastQC (version 0.11.5)