FastQCFastQC Report
Mon 16 Jan 2017
ERR1146527_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146527_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9513480
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT124665713.104111219028159No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3178103.3406282453949556No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2588502.7208760621770374No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC2245622.3604611561699818No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1989512.0912536737345326No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1950932.0507006899683393No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1863051.958326500922901No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC1459421.53405483587499No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1019351.071479626803231No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT985321.0357093303396865No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT802190.8432140499585852No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA495980.5213444501906768No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG474110.49835601693596876No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT414620.4358236943789234No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC414060.43523505594167433No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT335680.352846697528139No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA320440.336827322914433No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA314440.33052048251533617No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG265010.27856262902744316No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT200260.2105013097205229No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC197510.20761067453760346No Hit
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG119000.12508566791542106No Hit
CAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA118760.12483339429945718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGC230850.039.8981552
GAGTACG232050.039.7945861
GTACGCA232650.039.549073
TACGCAG236500.038.8058974
CGCAGTG245150.037.3010946
ACGCAGT253250.036.1462975
AGTAAGG34900.032.59450594
GCTTATG24800.032.040491
TTATGTA26650.029.799993
TATGTAC27350.028.5218264
TCTCGGG14350.027.51578194
GTAGGCA91500.026.7059973
CTTATGT29600.026.6712992
TAGGCAG92800.026.230614
TTAGGTA5750.026.152233
AGTAGGC93800.025.9509662
GCTTAGG6250.025.5777871
TAAGGGT2051.0859367E-925.2153764
GAGTAGG99500.025.1392691
CTTAGGT6000.025.0625532
Produced by FastQC (version 0.11.5)