##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146527_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9513480
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.234655562423	33.0	31.0	34.0	31.0	34.0
2	32.271502646770685	34.0	31.0	34.0	31.0	34.0
3	32.437149707572836	34.0	31.0	34.0	31.0	34.0
4	35.68356910405025	37.0	35.0	37.0	35.0	37.0
5	35.851100018079606	37.0	35.0	37.0	35.0	37.0
6	36.082692978804815	37.0	37.0	37.0	35.0	37.0
7	36.14630314038606	37.0	37.0	37.0	35.0	37.0
8	36.12120559458789	37.0	37.0	37.0	35.0	37.0
9	37.84656476914862	39.0	38.0	39.0	35.0	39.0
10-11	37.820053545074984	39.0	38.5	39.0	36.0	39.0
12-13	37.856830045367204	39.0	38.0	39.0	36.0	39.0
14-15	38.99847211535632	40.0	38.5	41.0	36.0	41.0
16-17	39.28941402094712	40.5	39.5	41.0	37.0	41.0
18-19	39.44057374378251	41.0	40.0	41.0	37.0	41.0
20-21	39.386106030600786	41.0	40.0	41.0	37.0	41.0
22-23	39.31982224170335	41.0	39.5	41.0	37.0	41.0
24-25	39.1755767605545	40.5	39.0	41.0	36.5	41.0
26-27	38.845860137405026	40.5	38.5	41.0	35.5	41.0
28-29	38.85186656197311	40.0	38.5	41.0	36.0	41.0
30-31	39.00301556317982	40.5	39.0	41.0	36.0	41.0
32-33	38.80750703212705	40.0	38.0	41.0	35.0	41.0
34-35	38.31081192161018	40.0	38.0	41.0	34.0	41.0
36-37	38.04931602315872	40.0	37.5	41.0	33.5	41.0
38-39	37.84228415889874	39.5	38.0	41.0	33.0	41.0
40-41	38.175190466580055	40.0	38.0	41.0	33.5	41.0
42-43	37.88754152003263	40.0	38.0	41.0	33.0	41.0
44-45	37.52937889184609	40.0	37.0	41.0	32.0	41.0
46-47	37.1328129138864	39.0	36.5	40.5	31.0	41.0
48-49	36.729047835282145	39.0	36.0	40.0	30.0	41.0
50-51	36.20796249111787	38.0	35.0	40.0	28.5	41.0
52-53	36.40184433036071	38.5	35.5	40.0	29.0	41.0
54-55	36.1237736874414	38.5	35.0	40.0	28.5	41.0
56-57	35.07294691322208	38.0	33.0	40.0	25.5	41.0
58-59	34.62140525864352	38.0	32.0	40.0	26.0	41.0
60-61	33.85064561022885	36.5	31.0	39.0	22.5	40.5
62-63	34.21225881591174	36.5	31.5	39.0	25.5	40.0
64-65	34.062731776384666	36.0	32.0	39.0	26.0	39.5
66-67	33.327034954611776	36.0	31.5	38.5	24.0	39.0
68-69	32.40244442622468	35.0	29.5	37.0	22.5	39.0
70-71	31.42027570352805	34.0	29.0	36.0	21.5	38.0
72-73	31.028259112333238	34.0	29.5	36.0	21.0	37.0
74-75	31.082837825905976	34.0	29.0	35.0	21.0	37.0
76-77	30.973738526806173	34.0	29.0	35.0	21.0	36.5
78-79	30.389435148862457	34.0	29.0	35.0	20.0	36.0
80-81	29.378413314580996	32.0	26.5	34.5	18.0	35.5
82-83	29.00902261843195	31.5	26.0	34.0	18.0	35.0
84-85	29.095484459945254	32.0	26.5	34.0	18.5	35.0
86-87	29.800207915505155	33.0	29.0	34.5	19.5	35.0
88-89	29.55402481531469	33.0	28.0	35.0	18.5	35.0
90-91	28.987456430244244	32.0	26.0	34.0	18.0	35.0
92-93	28.54812849766857	32.0	25.0	34.0	15.0	35.0
94-95	28.443772205333907	32.0	25.0	34.0	14.5	35.0
96-97	28.401306146646654	32.0	25.0	34.0	14.0	35.0
98-99	28.889494906175237	32.0	25.0	34.0	17.5	35.0
100	27.37726468127331	31.0	25.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24165766123121557
1101	2	0.2602476852501283
1101	3	0.2807285843806966
1101	4	0.23407543361997085
1101	5	0.15009466377868108
1101	6	0.13477285125848937
1101	7	0.15546684930255594
1101	8	0.16959267979223824
1101	9	0.264675850886519
1101	10-11	0.2949742701463194
1101	12-13	0.24546839234529472
1101	14-15	0.416571582487137
1101	16-17	0.3653632692809623
1101	18-19	0.3044997004870993
1101	20-21	0.34897821873849466
1101	22-23	0.3536056642802876
1101	24-25	0.40500265289173853
1101	26-27	0.425006950302631
1101	28-29	0.3983813244659089
1101	30-31	0.3997181473368414
1101	32-33	0.43641986587219606
1101	34-35	0.4977692802356657
1101	36-37	0.5557151396992595
1101	38-39	0.5364012242255356
1101	40-41	0.4932877774034736
1101	42-43	0.5573908997838402
1101	44-45	0.5762146465757496
1101	46-47	0.6424453163560315
1101	48-49	0.694425278175963
1101	50-51	0.7130107114205302
1101	52-53	0.6354197557177415
1101	54-55	0.7062548112146843
1101	56-57	0.7660538515285893
1101	58-59	0.8547733553553911
1101	60-61	0.979182147261696
1101	62-63	0.826457762201855
1101	64-65	0.7505918024627647
1101	66-67	0.7443642320093034
1101	68-69	0.7790920434351563
1101	70-71	0.7896127928871834
1101	72-73	0.775600187127548
1101	74-75	0.7376098233451955
1101	76-77	0.6720781920308703
1101	78-79	0.7182302438662589
1101	80-81	0.8181151564909506
1101	82-83	0.8445571249621295
1101	84-85	0.8084883424002598
1101	86-87	0.7275492078745458
1101	88-89	0.6936842485832173
1101	90-91	0.7734679937146183
1101	92-93	0.876967637233804
1101	94-95	0.8453645226973912
1101	96-97	0.7297903170185087
1101	98-99	0.720836193790003
1101	100	0.6553511086762214
1102	1	0.2149196484359095
1102	2	0.2682665864314373
1102	3	0.2776691616500244
1102	4	0.1961843272701529
1102	5	0.1666123051972832
1102	6	0.1253497677840656
1102	7	0.11473907187941279
1102	8	0.17525680234791707
1102	9	0.2606534494578696
1102	10-11	0.2436217857793821
1102	12-13	0.2107600328140009
1102	14-15	0.3573097364290021
1102	16-17	0.31826925403856166
1102	18-19	0.2794265921625012
1102	20-21	0.2849430741538015
1102	22-23	0.3344486324307283
1102	24-25	0.32398096317692193
1102	26-27	0.39779675732680175
1102	28-29	0.39179151845602433
1102	30-31	0.35393394105570763
1102	32-33	0.3730724421373708
1102	34-35	0.4254756525799195
1102	36-37	0.49663095681300007
1102	38-39	0.4310469736385443
1102	40-41	0.433108657839405
1102	42-43	0.41971152637094633
1102	44-45	0.43483911640103656
1102	46-47	0.5284060284678702
1102	48-49	0.6454143287471723
1102	50-51	0.6617366762638142
1102	52-53	0.6476887401923435
1102	54-55	0.6481314571666275
1102	56-57	0.7388201410161273
1102	58-59	0.8493503030539244
1102	60-61	0.8392678705776078
1102	62-63	0.6943150529110724
1102	64-65	0.6857211582853964
1102	66-67	0.7194211970919397
1102	68-69	0.7855186806002337
1102	70-71	0.7751615973777248
1102	72-73	0.7871415164178224
1102	74-75	0.6880773468743051
1102	76-77	0.645776008181258
1102	78-79	0.6506843265822795
1102	80-81	0.8402050518578292
1102	82-83	0.7871934638325087
1102	84-85	0.7238469020779981
1102	86-87	0.6376498926985974
1102	88-89	0.6371895683561171
1102	90-91	0.7574322791965962
1102	92-93	0.9149548051066532
1102	94-95	0.8429770551781886
1102	96-97	0.7322305491250845
1102	98-99	0.6664348740627446
1102	100	0.5372261742055997
1103	1	0.15872370669011104
1103	2	0.15064497788874576
1103	3	0.20779817361224673
1103	4	0.1034012577236254
1103	5	0.10545720214912535
1103	6	0.0687160274159524
1103	7	0.0647407226957597
1103	8	0.10823203701903594
1103	9	0.2005374967555369
1103	10-11	0.17459726645137863
1103	12-13	0.17391772628807445
1103	14-15	0.2596977565850267
1103	16-17	0.23306567781210674
1103	18-19	0.18531702788018833
1103	20-21	0.22533999101369773
1103	22-23	0.23608970470957757
1103	24-25	0.24463179047781836
1103	26-27	0.27016304731670004
1103	28-29	0.2826635793383474
1103	30-31	0.2525167032579816
1103	32-33	0.29087898730253414
1103	34-35	0.3807017982039298
1103	36-37	0.39027872183628176
1103	38-39	0.38175580387380137
1103	40-41	0.33598697842592173
1103	42-43	0.37811349972650277
1103	44-45	0.46434126474844817
1103	46-47	0.4561392765896599
1103	48-49	0.5899583900190919
1103	50-51	0.5694167590608927
1103	52-53	0.5346179817376608
1103	54-55	0.5673697864532912
1103	56-57	0.7185405998569436
1103	58-59	0.8222802976479713
1103	60-61	0.7538035597384081
1103	62-63	0.6721916283108982
1103	64-65	0.6580402710808642
1103	66-67	0.5846708603360398
1103	68-69	0.6574167607938577
1103	70-71	0.6974999457723854
1103	72-73	0.6722714944133159
1103	74-75	0.6028321613034109
1103	76-77	0.5771389455518907
1103	78-79	0.5817674160236699
1103	80-81	0.7330587436467475
1103	82-83	0.703516959186512
1103	84-85	0.6232006433648252
1103	86-87	0.6119576211348985
1103	88-89	0.48498109109251075
1103	90-91	0.6804078333977088
1103	92-93	0.7003440543100616
1103	94-95	0.7606743035411441
1103	96-97	0.5073178735882813
1103	98-99	0.447026441575872
1103	100	0.5760512081948725
1104	1	0.14483084963741533
1104	2	0.18342062904302736
1104	3	0.20591050359929142
1104	4	0.13464264571305762
1104	5	0.12315840616999907
1104	6	0.10810523631230495
1104	7	0.11255730413739684
1104	8	0.13200147534398354
1104	9	0.19696517927258128
1104	10-11	0.2012327744318867
1104	12-13	0.1753530152636671
1104	14-15	0.2641219584988619
1104	16-17	0.27929865978897794
1104	18-19	0.22558207735758629
1104	20-21	0.23071995470780138
1104	22-23	0.26631265628213185
1104	24-25	0.2765713766550846
1104	26-27	0.27226742021794337
1104	28-29	0.27947155990956674
1104	30-31	0.30848192642724825
1104	32-33	0.305763588552864
1104	34-35	0.32589881364507534
1104	36-37	0.37492452718683467
1104	38-39	0.36397714111586055
1104	40-41	0.3916943364133374
1104	42-43	0.3903453887432846
1104	44-45	0.39484140436880466
1104	46-47	0.4399417881443597
1104	48-49	0.5400426932816558
1104	50-51	0.4932819094345646
1104	52-53	0.5102505661910683
1104	54-55	0.5772200641524847
1104	56-57	0.5575059375555895
1104	58-59	0.6778890284294334
1104	60-61	0.6519160943087954
1104	62-63	0.6702282469140783
1104	64-65	0.6173280981715408
1104	66-67	0.6235176155577307
1104	68-69	0.6929403331668027
1104	70-71	0.6967129403792072
1104	72-73	0.6538665734289388
1104	74-75	0.5720671851215755
1104	76-77	0.5790924895367588
1104	78-79	0.5851133269006681
1104	80-81	0.5723454886221155
1104	82-83	0.6324975078123849
1104	84-85	0.6273486111240345
1104	86-87	0.5634460905874406
1104	88-89	0.5132977767099796
1104	90-91	0.6343769318854626
1104	92-93	0.6470753329160495
1104	94-95	0.6519784815795475
1104	96-97	0.5533774509698297
1104	98-99	0.4688145927974645
1104	100	0.4683476341163555
1105	1	0.10576720368894144
1105	2	0.1400955228194931
1105	3	0.12848345677147677
1105	4	0.10082391278680092
1105	5	0.07548847005977422
1105	6	0.04502367790562545
1105	7	0.015529752371215011
1105	8	0.04066491651911264
1105	9	0.09358997623736798
1105	10-11	0.10196285780115488
1105	12-13	0.0867730668239659
1105	14-15	0.19897747697103796
1105	16-17	0.15145929225560906
1105	18-19	0.10615140809691326
1105	20-21	0.17582121461994404
1105	22-23	0.14359275147553063
1105	24-25	0.16879123625700743
1105	26-27	0.18988579384991056
1105	28-29	0.18487356879649042
1105	30-31	0.1884675580065931
1105	32-33	0.23710189165062445
1105	34-35	0.1890067949001022
1105	36-37	0.27052696061331716
1105	38-39	0.2900785491552256
1105	40-41	0.2847501210968275
1105	42-43	0.2962983335827545
1105	44-45	0.2577513089276664
1105	46-47	0.28882510695967767
1105	48-49	0.3367960570770947
1105	50-51	0.3442415308999216
1105	52-53	0.30357901442813784
1105	54-55	0.3684506171764994
1105	56-57	0.3751557702925439
1105	58-59	0.4621033840420026
1105	60-61	0.4437715407541347
1105	62-63	0.4450162195346081
1105	64-65	0.4555664670091488
1105	66-67	0.40280821560907043
1105	68-69	0.4205288075678624
1105	70-71	0.42871706953591016
1105	72-73	0.47239068264578066
1105	74-75	0.4607739602499379
1105	76-77	0.3485168258969189
1105	78-79	0.30644729324101405
1105	80-81	0.3929386255135974
1105	82-83	0.36907594126714827
1105	84-85	0.3833906560526046
1105	86-87	0.40613438631440957
1105	88-89	0.33019599203573335
1105	90-91	0.4181125791995193
1105	92-93	0.39346475631355204
1105	94-95	0.3983177116629335
1105	96-97	0.3433137927548735
1105	98-99	0.30422275523494946
1105	100	0.37632508183031277
1106	1	0.10243974563388036
1106	2	0.11009954422775081
1106	3	0.1212027344296871
1106	4	0.06913402774364386
1106	5	0.052958629820189174
1106	6	0.025638610256308425
1106	7	0.03002402128243631
1106	8	0.059242645610737554
1106	9	0.08998309960530548
1106	10-11	0.10479654945241634
1106	12-13	0.0873983780762444
1106	14-15	0.1678446830557121
1106	16-17	0.13282309576501916
1106	18-19	0.11105746960564034
1106	20-21	0.07949552484877387
1106	22-23	0.1364689321563688
1106	24-25	0.16359853817534997
1106	26-27	0.14625962803478387
1106	28-29	0.16372480260383782
1106	30-31	0.16397718605168166
1106	32-33	0.1710821517861092
1106	34-35	0.17669348126687368
1106	36-37	0.2283383182512324
1106	38-39	0.22589431247946123
1106	40-41	0.21491440029121378
1106	42-43	0.1951429124739974
1106	44-45	0.21524771826025102
1106	46-47	0.3287963178713511
1106	48-49	0.26951227043221593
1106	50-51	0.35936082821806536
1106	52-53	0.30751613477216466
1106	54-55	0.3744874998629726
1106	56-57	0.3718521093228233
1106	58-59	0.45134441443372
1106	60-61	0.45573396296430957
1106	62-63	0.4132626562107191
1106	64-65	0.3449082523564613
1106	66-67	0.39036963955790327
1106	68-69	0.33710744224329403
1106	70-71	0.4056422243681759
1106	72-73	0.41466688957901354
1106	74-75	0.3436728057643954
1106	76-77	0.32017028720283847
1106	78-79	0.33958165864514456
1106	80-81	0.38391818665117583
1106	82-83	0.3446299488366549
1106	84-85	0.3289418205911261
1106	86-87	0.3252913413588132
1106	88-89	0.3658359484766791
1106	90-91	0.36309020406481096
1106	92-93	0.35432971786801204
1106	94-95	0.35856190924816644
1106	96-97	0.3590693996582246
1106	98-99	0.34297294993590555
1106	100	0.2649129591957866
1107	1	0.0810494145815639
1107	2	0.09808676891966428
1107	3	0.1643908962005014
1107	4	0.132956857727919
1107	5	0.10297005477916343
1107	6	0.05394538488277334
1107	7	0.04953116708200156
1107	8	0.05604129829658433
1107	9	0.09561929226495636
1107	10-11	0.11468942323554643
1107	12-13	0.0935570271160131
1107	14-15	0.16572683064244131
1107	16-17	0.1196072054355497
1107	18-19	0.10353852783917716
1107	20-21	0.12376634535903008
1107	22-23	0.1367431440754956
1107	24-25	0.16575896772283727
1107	26-27	0.16215852042539325
1107	28-29	0.13979268786741272
1107	30-31	0.14704263545309715
1107	32-33	0.15432577325499608
1107	34-35	0.16730830200134506
1107	36-37	0.17909681474831274
1107	38-39	0.17933614653454555
1107	40-41	0.19390151096580865
1107	42-43	0.2037207623818773
1107	44-45	0.24533736199302325
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2216	90-91	-2.793420612067276
2216	92-93	-3.0383174269575157
2216	94-95	-3.074209964223698
2216	96-97	-2.5777245827024835
2216	98-99	-2.5333785261887876
2216	100	-2.5869577710947276
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	257.0
1	126.0
2	132.0
3	113.0
4	94.0
5	125.0
6	336.0
7	1757.0
8	4942.0
9	5892.0
10	5247.0
11	5742.0
12	7293.0
13	8256.0
14	9185.0
15	10436.0
16	11576.0
17	13640.0
18	16012.0
19	19566.0
20	24723.0
21	31352.0
22	39807.0
23	51065.0
24	65133.0
25	81617.0
26	101372.0
27	124035.0
28	153845.0
29	195196.0
30	249681.0
31	327551.0
32	448671.0
33	643434.0
34	964646.0
35	1436913.0
36	1878484.0
37	1805986.0
38	706246.0
39	62915.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	56.99083665376359	14.435922681682658	12.1426196624936	16.430621002060153
2	11.467823457927025	31.874161631197623	26.0407862480184	30.61722866285695
3	44.39285294154441	17.687164652456975	27.32852182529775	10.591460580700865
4	9.319972566703614	11.396655021672473	51.01986559198565	28.263506819638273
5	8.970931567425458	45.89873629039922	33.754201922729195	11.376130219446125
6	23.620829093643163	37.83246328355598	13.31668680026715	25.23002082253371
7	24.31058438578304	34.477192852627326	22.25936125397106	18.95286150761857
8	19.78611919189838	12.404622240685919	19.46467356854784	48.34458499886786
9	12.91838163919592	32.52791558645385	34.10719004926632	20.446512725083902
10-11	29.30239711278013	16.234866867539473	25.88027271411345	28.58246330556694
12-13	42.58408981858703	18.29341678204936	19.573051711787198	19.54944168757641
14-15	25.84519328146026	23.55445803046692	38.14038739603365	12.459961292039164
16-17	17.5602463039765	26.63812333479929	33.17057473888848	22.631055622335726
18-19	14.618226718794727	43.274977827073144	22.277397427677677	19.829398026454452
20-21	24.166712876225745	24.405348675432553	16.23470846849082	35.193229979850884
22-23	16.99363471230118	27.142907223444467	23.628310170085612	32.23514789416874
24-25	32.893171068315596	24.45027895946446	19.259153713021927	23.39739625919802
26-27	27.520686563758	15.034954541409839	38.1290452216512	19.315313673180963
28-29	16.41308800593048	40.92996736113819	23.996617012301094	18.66032762063023
30-31	21.750663617946966	22.7705869261982	21.645508648662457	33.833240807192375
32-33	25.578339270786554	25.035169528586398	26.818944260265653	22.567546940361392
34-35	34.3277678535526	19.716248048919525	25.0285010839847	20.92748301354317
36-37	26.390198525970977	16.61760981387973	37.39681844135356	19.595373218795736
38-39	20.83297697455312	28.837111454815904	31.899566382110567	18.43034518852041
40-41	20.45506992806651	33.26962059265307	24.544164173334682	21.73114530594574
42-43	16.766794856684385	32.29623554103551	15.769064931326326	35.16790467095378
44-45	24.30274657771936	19.218447408127993	20.122109608135467	36.35669640601718
46-47	33.630880671600735	29.062455657709563	22.824775881347957	14.48188778934174
48-49	28.256661834084717	25.074507562422955	25.764669872405538	20.904160731086794
50-51	10.560575598663208	28.341885308115828	40.96446654872181	20.133072544499157
52-53	23.00331400949726	35.358508786152285	19.128279232270238	22.50989797208022
54-55	16.200361212500912	22.463887556507476	27.425393782457245	33.91035744853437
56-57	28.789107920496654	16.43166621318054	25.969954243907186	28.809271622415622
58-59	41.067770368219016	18.847530867183362	20.019452282443904	20.06524648215372
60-61	25.43111749560285	23.66574255460089	37.64259886552358	13.260541084272678
62-63	17.862517111730252	26.23345094980815	32.86559956274725	23.038432375714347
64-65	14.997765264379922	42.04948573741307	22.53111398140088	20.42163501680613
66-67	23.98225278876824	24.22277085583075	16.794413520200578	35.000562835200434
68-69	17.10937672522848	26.891675894174423	23.928068553810302	32.070878826786796
70-71	32.14278710115277	24.439456991039776	19.572637893093855	23.845118014713595
72-73	26.93799036509546	15.503681070232764	37.33692702191776	20.221401542754023
74-75	16.695162872655214	39.90241905001559	24.167544757112587	19.234873320216614
76-77	21.87021725797475	22.57476581339574	22.02231860840067	33.53269832022884
78-79	25.385305032625062	24.63076774567218	26.965200778812072	23.018726442890692
80-81	33.62135319912211	19.84551413186523	25.063969507688356	21.469163161324303
82-83	25.987634399952302	17.026076027714236	36.5922347850931	20.39405478724036
84-85	20.852739400851323	28.379105767988165	31.410405980908564	19.357748850251948
86-87	20.633480337548722	32.54653140028652	24.719404698383215	22.10058356378154
88-89	17.4204723828242	31.56057595587523	16.4168905993285	34.60206106197207
90-91	24.305215097352455	19.32316070947982	20.52488345880217	35.84674073436556
92-93	32.753664532640485	28.51306890153636	23.213011788659326	15.520254777163828
94-95	27.689574141231276	24.88193431814026	25.729469831432983	21.699021709195478
96-97	11.410717263711714	27.922038866373946	39.743787371614516	20.92345649829983
98-99	23.183204976739354	34.356611083095245	19.66535562227958	22.79482831788582
100	20.594193574232243	12.590977420366597	20.717506809933486	46.09732219546767
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	376.0
1	284.0
2	192.0
3	194.0
4	176.0
5	132.0
6	107.5
7	119.5
8	142.0
9	143.5
10	129.0
11	133.5
12	147.0
13	173.0
14	212.5
15	232.5
16	258.5
17	297.5
18	335.5
19	362.5
20	399.0
21	445.5
22	545.0
23	760.0
24	1022.0
25	1351.5
26	1966.0
27	2816.0
28	3785.5
29	5160.5
30	6804.5
31	8524.5
32	10558.0
33	12638.5
34	14531.0
35	16627.5
36	18999.0
37	21532.5
38	23339.5
39	24414.0
40	25393.5
41	26308.0
42	28001.0
43	31156.5
44	36693.5
45	53491.5
46	138770.0
47	950134.0
48	2853732.0
49	3011094.0
50	1307649.0
51	407028.5
52	147281.5
53	75695.5
54	51334.0
55	37668.0
56	29656.0
57	23910.5
58	19754.0
59	17607.0
60	14632.0
61	11142.0
62	8518.0
63	6827.0
64	5207.5
65	3475.0
66	2429.0
67	1805.5
68	1429.0
69	1096.0
70	859.5
71	698.0
72	539.5
73	475.0
74	390.5
75	267.5
76	201.5
77	156.5
78	116.5
79	86.5
80	76.0
81	61.5
82	48.0
83	48.5
84	46.0
85	30.0
86	23.0
87	27.5
88	26.0
89	20.5
90	18.5
91	21.0
92	19.5
93	12.5
94	9.0
95	11.5
96	12.0
97	10.5
98	12.0
99	14.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06230107174241182
2	0.010090944638554977
3	0.012056576562940164
4	0.010006853433233686
5	0.012277315976908556
6	0.012498055390876945
7	0.0029221693849148787
8	0.005444905544553623
9	0.0072949120616220345
10-11	0.006948035839671708
12-13	0.00600726548013976
14-15	0.010159268742878525
16-17	0.009728301315606907
18-19	0.00791508470086656
20-21	0.006911245937343643
22-23	0.002538503260636486
24-25	0.003778848539125536
26-27	0.005061239420275231
28-29	0.0061701921904497616
30-31	0.008966224767382703
32-33	0.010343218254518852
34-35	0.011799047246643709
36-37	0.018153188948733797
38-39	0.01854736647367735
40-41	0.018678758981991866
42-43	0.02458616615581259
44-45	0.028175809482965223
46-47	0.030992864861228486
48-49	0.031912612419430116
50-51	0.03262213196432851
52-53	0.030966586359565586
54-55	0.03747839907163309
56-57	0.04012727203925378
58-59	0.0372313811560018
60-61	0.038161640114868584
62-63	0.03354187952253014
64-65	0.035807086365872424
66-67	0.03775169548892729
68-69	0.04067912057417475
70-71	0.04035852285388732
72-73	0.03651660591077082
74-75	0.03925482578404537
76-77	0.03891846096276021
78-79	0.0379671792025631
80-81	0.04274461080487897
82-83	0.03900780786841408
84-85	0.03524472643028629
86-87	0.033725829034170464
88-89	0.03147638929182592
90-91	0.03380992023949175
92-93	0.035376118938600806
94-95	0.03921803588171731
96-97	0.0342040977644353
98-99	0.031492156392823656
100	0.028811749223207492
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9513480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.848785027264164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40797117737183	39.58390884671997
2	13.419448012945276	13.378863583637127
3	4.007672596042426	5.993328290993298
4	1.5825185720654464	3.15546512402174
5	0.7130029426461022	1.7771165205886155
6	0.35100369794447617	1.0498264729565374
7	0.18703781141888204	0.6526525357372877
8	0.10880050049149156	0.4338858207887289
9	0.07452891153173836	0.3343658120335453
>10	0.14638157547249253	0.9535168888953683
>50	3.323477820410609E-4	0.009826959523323249
>100	3.1509320009103593E-4	0.04456528084242374
>500	1.4897599635464771E-4	0.05238001184348666
>1k	2.97879143579099E-4	0.4569938707847062
>5k	1.1170467883846611E-4	0.36664751335834306
>10k+	4.282012688807868E-4	31.756656467275484
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1246657	13.104111219028159	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	317810	3.3406282453949556	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	258850	2.7208760621770374	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	224562	2.3604611561699818	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	198951	2.0912536737345326	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	195093	2.0507006899683393	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	186305	1.958326500922901	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	145942	1.53405483587499	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	101935	1.071479626803231	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	98532	1.0357093303396865	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	80219	0.8432140499585852	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	49598	0.5213444501906768	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	47411	0.49835601693596876	No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	41462	0.4358236943789234	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	41406	0.43523505594167433	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	33568	0.352846697528139	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	32044	0.336827322914433	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	31444	0.33052048251533617	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	26501	0.27856262902744316	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	20026	0.2105013097205229	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	19751	0.20761067453760346	No Hit
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	11900	0.12508566791542106	No Hit
CAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	11876	0.12483339429945718	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.0511400665161434E-5	0.0
4	0.0	0.0	0.0	1.0511400665161434E-5	0.0
5	0.0	0.0	0.0	1.0511400665161434E-5	0.0
6	0.0	0.0	0.0	1.0511400665161434E-5	0.0
7	0.0	0.0	0.0	1.0511400665161434E-5	0.0
8	0.0	0.0	0.0	2.1022801330322867E-5	0.0
9	0.0	0.0	0.0	2.3125081463355154E-4	0.0
10-11	0.0	0.0	0.0	7.463094472264618E-4	0.0
12-13	0.0	0.0	0.0	0.0014242947901293742	0.0
14-15	0.0	0.0	0.0	0.0027750097756026187	0.0
16-17	0.0	0.0	0.0	0.005434394143888461	0.0
18-19	0.0	0.0	0.0	0.00886636646106367	0.0
20-21	0.0	0.0	0.0	0.015935283408384734	0.0
22-23	0.0	0.0	0.0	0.025847534235631967	0.0
24-25	0.0	0.0	0.0	0.03398335835046692	0.0
26-27	0.0	0.0	0.0	0.04002215803260216	0.0
28-29	0.0	0.0	0.0	0.05460672645551365	0.0
30-31	0.0	0.0	0.0	0.0690966922724387	0.0
32-33	0.0	0.0	0.0	0.12479660439712911	0.0
34-35	0.0	0.0	0.0	0.18605704747368995	0.0
36-37	0.0	0.0	0.0	0.22557465827436438	0.0
38-39	0.0	0.0	0.0	0.28287230330015933	0.0
40-41	0.0	0.0	0.0	0.3389558815491282	0.0
42-43	0.0	0.0	0.0	0.41142147773475113	0.0
44-45	0.0	0.0	0.0	0.5019193817614584	0.0
46-47	0.0	0.0	0.0	0.6210398297994004	0.0
48-49	0.0	0.0	0.0	0.6863892077347091	0.0
50-51	0.0	0.0	0.0	0.7681311150073369	0.0
52-53	0.0	0.0	0.0	0.8492423382400551	0.0
54-55	0.0	0.0	0.0	0.967111929598843	0.0
56-57	0.0	0.0	0.0	1.1803356920916426	0.0
58-59	0.0	0.0	0.0	1.304590959354516	0.0
60-61	0.0	1.0511400665161434E-5	0.0	1.4162220344185303	0.0
62-63	0.0	1.0511400665161434E-5	0.0	1.5269543847256735	0.0
64-65	0.0	1.0511400665161434E-5	0.0	1.6338290509887023	0.0
66-67	0.0	1.0511400665161434E-5	0.0	1.7889352792038244	0.0
68-69	0.0	1.0511400665161434E-5	0.0	1.9724433120162128	0.0
70-71	0.0	1.0511400665161434E-5	0.0	2.1251687079806763	0.0
72-73	0.0	1.0511400665161434E-5	0.0	2.234392672292368	0.0
74-75	0.0	1.0511400665161434E-5	0.0	2.399758027556688	0.0
76-77	0.0	1.0511400665161434E-5	0.0	2.520244957681101	0.0
78-79	0.0	1.0511400665161434E-5	0.0	2.8347986225860566	0.0
80-81	0.0	1.0511400665161434E-5	0.0	3.1260642793173474	0.0
82-83	0.0	1.0511400665161434E-5	0.0	3.2740490335818224	0.0
84-85	0.0	1.0511400665161434E-5	0.0	3.4515498009140715	0.0
86-87	0.0	1.0511400665161434E-5	0.0	3.6198583483646365	0.0
88	0.0	1.0511400665161434E-5	0.0	3.782969008186279	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACGC	23085	0.0	39.898155	2
GAGTACG	23205	0.0	39.794586	1
GTACGCA	23265	0.0	39.54907	3
TACGCAG	23650	0.0	38.805897	4
CGCAGTG	24515	0.0	37.301094	6
ACGCAGT	25325	0.0	36.146297	5
AGTAAGG	3490	0.0	32.594505	94
GCTTATG	2480	0.0	32.04049	1
TTATGTA	2665	0.0	29.79999	3
TATGTAC	2735	0.0	28.521826	4
TCTCGGG	1435	0.0	27.515781	94
GTAGGCA	9150	0.0	26.705997	3
CTTATGT	2960	0.0	26.671299	2
TAGGCAG	9280	0.0	26.23061	4
TTAGGTA	575	0.0	26.15223	3
AGTAGGC	9380	0.0	25.950966	2
GCTTAGG	625	0.0	25.577787	1
TAAGGGT	205	1.0859367E-9	25.215376	4
GAGTAGG	9950	0.0	25.139269	1
CTTAGGT	600	0.0	25.062553	2
>>END_MODULE