FastQCFastQC Report
Mon 16 Jan 2017
ERR1146527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9513480
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT130948013.764468943015595No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5315575.587408603371216No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3330133.5004330697074044No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2886583.0341998932041694No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC2561592.692589882987088No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2218132.3315653157414533No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2164372.275056025765545No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2065272.1708880451737955No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC1644101.7281793833591914No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1157941.217157128621703No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT1109321.1660506985876884No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT897800.9437135517181936No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA569100.5982038118543372No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG545780.5736912255031807No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT499000.5245188931915555No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA375300.39449286696350866No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC368590.38743971711718533No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT349910.36780442067466373No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG298850.31413320887834945No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC232250.2441272804483743No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT218540.229716150136438No Hit
CAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA147700.15525338782443437No Hit
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG143000.1503130295118085No Hit
GTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA106110.11153647245802799No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTAT97040.10200263205472657No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA96620.10156115322678977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG272100.036.2508351
GTACGCA273950.035.8697363
AGTACGC275000.035.7299582
TACGCAG274850.035.7180674
CGCAGTG283900.034.698826
ACGCAGT291250.033.7713935
GCTTATG26200.028.7391551
AGCTTAC18400.028.645441
GACGTCC856.822478E-427.6451767
GTACCGT909.5239433E-426.1105676
TTATGTA29450.025.863243
AGTAAGG9700.025.1946994
TATGTAC30850.024.9943544
GTAGGCA99450.024.5366783
TAGGCAG99700.024.3336774
ACGCCTA1951.760418E-824.1020626
AGTAGGC102200.023.7592952
GAGTAGG107100.023.1566871
AGGCAGT106950.023.0797825
GGCAGTG107650.022.9210286
Produced by FastQC (version 0.11.5)