##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146527_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9513480
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50083796886103	33.0	31.0	34.0	31.0	34.0
2	32.45878669004402	34.0	31.0	34.0	31.0	34.0
3	32.72830215651896	34.0	31.0	34.0	31.0	34.0
4	35.85853862098833	37.0	35.0	37.0	35.0	37.0
5	35.97704635948149	37.0	35.0	37.0	35.0	37.0
6	36.270314332925494	37.0	37.0	37.0	35.0	37.0
7	36.33267836795789	37.0	37.0	37.0	35.0	37.0
8	36.36236035604216	37.0	37.0	37.0	35.0	37.0
9	38.13035324613075	39.0	39.0	39.0	37.0	39.0
10-11	38.10375183423942	39.0	38.5	39.0	36.0	39.0
12-13	38.14206657290497	39.0	39.0	39.0	37.0	39.0
14-15	39.223244490974906	40.0	38.5	41.0	36.5	41.0
16-17	39.53325575919642	40.5	39.5	41.0	37.0	41.0
18-19	39.689994302820836	41.0	40.0	41.0	37.0	41.0
20-21	39.68666592035722	41.0	39.5	41.0	37.0	41.0
22-23	39.59084152171445	41.0	39.0	41.0	37.0	41.0
24-25	39.452639044807995	40.5	39.0	41.0	36.5	41.0
26-27	39.14745455921492	40.5	38.5	41.0	36.5	41.0
28-29	39.20890846462073	40.0	38.5	41.0	36.5	41.0
30-31	39.437629237671175	41.0	39.0	41.0	37.0	41.0
32-33	39.308305846020595	41.0	39.0	41.0	36.0	41.0
34-35	38.994201123038046	40.0	38.5	41.0	35.5	41.0
36-37	38.787237004755355	40.0	38.5	41.0	35.0	41.0
38-39	38.678443902756925	40.0	38.0	41.0	35.0	41.0
40-41	39.050421507166675	40.0	39.0	41.0	35.5	41.0
42-43	39.00304462720267	40.0	38.5	41.0	35.0	41.0
44-45	38.86952245655638	40.0	38.0	41.0	35.0	41.0
46-47	38.69706900103853	40.0	38.0	41.0	35.0	41.0
48-49	38.402540552983766	40.0	38.0	41.0	33.5	41.0
50-51	38.013574633047	40.0	37.5	41.0	33.5	41.0
52-53	38.370136427469234	40.0	38.0	41.0	34.0	41.0
54-55	38.36361757211872	40.0	38.0	41.0	34.0	41.0
56-57	37.90334325609556	40.0	37.5	41.0	33.0	41.0
58-59	37.6264680222169	39.0	37.0	41.0	32.0	41.0
60-61	36.81974466756644	39.0	35.5	40.0	31.0	41.0
62-63	37.02350727599154	39.0	36.0	40.0	31.5	41.0
64-65	36.71130606255545	38.5	36.0	39.5	31.0	40.5
66-67	36.13295802377258	37.5	35.5	39.0	31.0	40.0
68-69	35.28983332071965	37.0	34.5	39.0	29.5	39.0
70-71	34.331321819145046	35.5	33.5	37.5	29.0	38.0
72-73	33.72560167257407	35.0	33.5	37.0	27.5	37.0
74-75	33.61994485719211	35.0	33.5	37.0	29.0	37.0
76-77	33.46153037584564	35.0	34.0	36.0	29.0	36.5
78-79	33.14576984447332	35.0	33.5	35.5	29.0	36.0
80-81	32.65769402994488	34.0	32.0	35.0	27.0	36.0
82-83	32.239634077120044	34.5	32.0	35.0	26.5	35.5
84-85	32.1492651479795	34.0	32.0	35.0	26.0	35.0
86-87	32.577161564432785	34.5	33.0	35.0	27.0	35.5
88-89	32.458447276916544	35.0	33.0	35.0	27.0	35.0
90-91	32.19570441100417	34.0	32.0	35.0	25.5	35.0
92-93	32.06301768648276	34.0	32.0	35.0	25.0	35.0
94-95	31.7642873060121	34.0	31.5	35.0	25.0	35.0
96-97	31.615318999987387	34.0	31.0	35.0	25.0	35.0
98-99	31.931307471083137	34.0	32.0	35.0	25.0	35.0
100	31.007093408510872	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29509921392514826
1101	2	0.2824795970698091
1101	3	0.28794529220702003
1101	4	0.19976463790790433
1101	5	0.19178791214939395
1101	6	0.181502901604496
1101	7	0.1614451066665481
1101	8	0.15288814992180733
1101	9	0.22060885549816334
1101	10-11	0.24509827457497835
1101	12-13	0.24302533140784988
1101	14-15	0.4025980425946116
1101	16-17	0.36693403668265034
1101	18-19	0.31985626585377247
1101	20-21	0.3337601778879815
1101	22-23	0.35002524208913144
1101	24-25	0.3555781161453524
1101	26-27	0.39884422469877023
1101	28-29	0.42265551096810583
1101	30-31	0.41521131975960657
1101	32-33	0.3843694648186258
1101	34-35	0.42642100450991194
1101	36-37	0.4842584005339532
1101	38-39	0.5232924023263124
1101	40-41	0.4688216886765275
1101	42-43	0.44909304653570814
1101	44-45	0.5060914122800426
1101	46-47	0.5247511941976484
1101	48-49	0.5471000423253045
1101	50-51	0.5246307767036527
1101	52-53	0.567133614454157
1101	54-55	0.540719153860195
1101	56-57	0.6060981073251384
1101	58-59	0.6548599651169695
1101	60-61	0.6597665990086696
1101	62-63	0.6177103301752211
1101	64-65	0.5372326494372004
1101	66-67	0.5406859362111973
1101	68-69	0.555123519382299
1101	70-71	0.557893414287868
1101	72-73	0.5057088192942416
1101	74-75	0.4511501849931605
1101	76-77	0.45618111557374164
1101	78-79	0.490411862734355
1101	80-81	0.593659742970928
1101	82-83	0.6332053506899626
1101	84-85	0.631140670925042
1101	86-87	0.587269215880653
1101	88-89	0.5619540629449062
1101	90-91	0.617286142942433
1101	92-93	0.6735494546740881
1101	94-95	0.6672199093076365
1101	96-97	0.6079283796227024
1101	98-99	0.6003935113793162
1101	100	0.6299494323402293
1102	1	0.26174366399597915
1102	2	0.25137887795825264
1102	3	0.2781279644143737
1102	4	0.17871163989536853
1102	5	0.13991460702442282
1102	6	0.15827758053396934
1102	7	0.1493506088081915
1102	8	0.14765331026441686
1102	9	0.19290951774317477
1102	10-11	0.23479097513401825
1102	12-13	0.2466537296884539
1102	14-15	0.3239860390620066
1102	16-17	0.31624512514121506
1102	18-19	0.2879234924376419
1102	20-21	0.2850777952502028
1102	22-23	0.31395131160854106
1102	24-25	0.3353875460973583
1102	26-27	0.34901030274485834
1102	28-29	0.37661864448354976
1102	30-31	0.3690114394682027
1102	32-33	0.35339987337246015
1102	34-35	0.40124617749921043
1102	36-37	0.4432838026707344
1102	38-39	0.45720945084114817
1102	40-41	0.4068001461719817
1102	42-43	0.3894790644706063
1102	44-45	0.42631429499614626
1102	46-47	0.47543283893740096
1102	48-49	0.47769315611495244
1102	50-51	0.4824787860823321
1102	52-53	0.4663405704577741
1102	54-55	0.48453937641147604
1102	56-57	0.5632848591119242
1102	58-59	0.6212741183495041
1102	60-61	0.5934121450394301
1102	62-63	0.5287154031276771
1102	64-65	0.4797582360760515
1102	66-67	0.45785412657719604
1102	68-69	0.38478490599334947
1102	70-71	0.4303085283147752
1102	72-73	0.4483596869445847
1102	74-75	0.4147316395695526
1102	76-77	0.39028824063350953
1102	78-79	0.4205691958123481
1102	80-81	0.47517716347766736
1102	82-83	0.49195430911045435
1102	84-85	0.5106459708255215
1102	86-87	0.49405807272193414
1102	88-89	0.4393739747422245
1102	90-91	0.4790301936740633
1102	92-93	0.521724579275002
1102	94-95	0.5378601349315595
1102	96-97	0.413302448885009
1102	98-99	0.4792318827436688
1102	100	0.551177233246456
1103	1	0.19567710318713694
1103	2	0.21493830330615538
1103	3	0.21255242178227007
1103	4	0.16164857881717865
1103	5	0.12880644723858836
1103	6	0.12572263147693263
1103	7	0.11799736632841729
1103	8	0.11879828990062435
1103	9	0.154566702893419
1103	10-11	0.17645113201952256
1103	12-13	0.1959379665562011
1103	14-15	0.26465532068202435
1103	16-17	0.24744571894597556
1103	18-19	0.23198029705477552
1103	20-21	0.2107630275841359
1103	22-23	0.2607065563132238
1103	24-25	0.2755165774653108
1103	26-27	0.3350231963382839
1103	28-29	0.33199503832015864
1103	30-31	0.32477790844806265
1103	32-33	0.30419104505119066
1103	34-35	0.3848497365316561
1103	36-37	0.3779208990075915
1103	38-39	0.4182561330033323
1103	40-41	0.3901004840018487
1103	42-43	0.3295946979087816
1103	44-45	0.3991546643515562
1103	46-47	0.45332905931760337
1103	48-49	0.4883172093545056
1103	50-51	0.47342793955646556
1103	52-53	0.4741388501104282
1103	54-55	0.4525293828318411
1103	56-57	0.5419848423007068
1103	58-59	0.5551632965226148
1103	60-61	0.6200752149113313
1103	62-63	0.5763100999080706
1103	64-65	0.4884641922427022
1103	66-67	0.4771922305659757
1103	68-69	0.4581709764847446
1103	70-71	0.47803638695980766
1103	72-73	0.5140711295103131
1103	74-75	0.44806651960172417
1103	76-77	0.4132187243583161
1103	78-79	0.4474997923030699
1103	80-81	0.5085307580784217
1103	82-83	0.5195362935416341
1103	84-85	0.5095847032622629
1103	86-87	0.432855884191369
1103	88-89	0.44019471092018847
1103	90-91	0.507680383882132
1103	92-93	0.4997127110485309
1103	94-95	0.5432079757594757
1103	96-97	0.5363466568429907
1103	98-99	0.4698599444630389
1103	100	0.46589709742121954
1104	1	0.22626261189332553
1104	2	0.2286491122726062
1104	3	0.24413482347060977
1104	4	0.18152654963941472
1104	5	0.10136572035059999
1104	6	0.1115585056131323
1104	7	0.1418480348128952
1104	8	0.11490072220757241
1104	9	0.16212521183377504
1104	10-11	0.17768190294167852
1104	12-13	0.20380840913414744
1104	14-15	0.33086802104948987
1104	16-17	0.2895449855961374
1104	18-19	0.24412193932079163
1104	20-21	0.2527212467648354
1104	22-23	0.2907125469364402
1104	24-25	0.3167670688539843
1104	26-27	0.31468191885743835
1104	28-29	0.3598058776233941
1104	30-31	0.33021444347925666
1104	32-33	0.32331280058473766
1104	34-35	0.36804122478994117
1104	36-37	0.4249009864068398
1104	38-39	0.4266604013108761
1104	40-41	0.3802865524928478
1104	42-43	0.3797851706003996
1104	44-45	0.3935264552456843
1104	46-47	0.4517193708124765
1104	48-49	0.4796244225640649
1104	50-51	0.4827630527607667
1104	52-53	0.4924903058179311
1104	54-55	0.4603186512153101
1104	56-57	0.5322673416803951
1104	58-59	0.5472463127880332
1104	60-61	0.5926750782297603
1104	62-63	0.5363640076819252
1104	64-65	0.5310190330472011
1104	66-67	0.47067520320602085
1104	68-69	0.5250839706202228
1104	70-71	0.5066508563849652
1104	72-73	0.5129438348546955
1104	74-75	0.43844638573008865
1104	76-77	0.4505592486864032
1104	78-79	0.44685400849284207
1104	80-81	0.4919977373998492
1104	82-83	0.539338363320681
1104	84-85	0.5306657329798696
1104	86-87	0.5266162699830801
1104	88-89	0.5238625649703579
1104	90-91	0.5419074792561602
1104	92-93	0.616140107646352
1104	94-95	0.6086421307836822
1104	96-97	0.552239897517449
1104	98-99	0.5501020472753879
1104	100	0.6059092570096141
1105	1	0.1731954128422757
1105	2	0.17403116730191925
1105	3	0.20333969895257553
1105	4	0.1308527715963237
1105	5	0.13838755380252366
1105	6	0.10958572505981579
1105	7	0.1391892665357659
1105	8	0.10790325154668778
1105	9	0.13293935880601992
1105	10-11	0.12598881937439188
1105	12-13	0.14470021509183084
1105	14-15	0.22253886671523304
1105	16-17	0.20634857919531413
1105	18-19	0.21443132750097504
1105	20-21	0.20725175835016785
1105	22-23	0.2231736633117123
1105	24-25	0.2257056423696966
1105	26-27	0.2419463963769104
1105	28-29	0.2826363121410438
1105	30-31	0.2750112330486729
1105	32-33	0.25199008635934916
1105	34-35	0.3039691652865102
1105	36-37	0.31792822197551374
1105	38-39	0.33462296630701616
1105	40-41	0.31531500940825197
1105	42-43	0.28975337115508637
1105	44-45	0.3185130507592717
1105	46-47	0.3800325434945435
1105	48-49	0.40868641569321085
1105	50-51	0.4024861289861761
1105	52-53	0.3667281675186089
1105	54-55	0.36726576045735726
1105	56-57	0.38345331643203906
1105	58-59	0.41242465852311483
1105	60-61	0.45816899897230456
1105	62-63	0.3989744620900879
1105	64-65	0.3824682551934018
1105	66-67	0.35375059658741037
1105	68-69	0.3623422516736525
1105	70-71	0.3181252103582537
1105	72-73	0.35607608480802355
1105	74-75	0.28601075953756094
1105	76-77	0.25978846378047393
1105	78-79	0.25545406443671936
1105	80-81	0.35686060279395093
1105	82-83	0.3570900198951108
1105	84-85	0.41532764473485884
1105	86-87	0.3749445305510335
1105	88-89	0.33274133336888667
1105	90-91	0.3661101571562426
1105	92-93	0.3598784479635313
1105	94-95	0.3370682170508239
1105	96-97	0.33940017352174934
1105	98-99	0.3184698770541772
1105	100	0.35566720630007254
1106	1	0.12479959313129996
1106	2	0.13633869457185455
1106	3	0.13589009965633636
1106	4	0.10010041012587578
1106	5	0.08296177591632414
1106	6	0.08181613106191321
1106	7	0.05019963344907552
1106	8	0.0851726128607666
1106	9	0.09210108001227013
1106	10-11	0.1087500530074621
1106	12-13	0.11917112619829595
1106	14-15	0.20446634294638244
1106	16-17	0.1920972635673266
1106	18-19	0.18055634240747054
1106	20-21	0.17335473851545657
1106	22-23	0.18209880613392926
1106	24-25	0.17379663855125926
1106	26-27	0.18974304844955725
1106	28-29	0.23566223637578076
1106	30-31	0.2422655896341226
1106	32-33	0.21203216917947287
1106	34-35	0.20166737738401963
1106	36-37	0.21382799994408686
1106	38-39	0.25894894745241714
1106	40-41	0.26863556603660754
1106	42-43	0.2805068191868543
1106	44-45	0.27390441071695193
1106	46-47	0.2848613686391346
1106	48-49	0.25114575085301283
1106	50-51	0.23554665434619437
1106	52-53	0.26429450840762314
1106	54-55	0.284925734691015
1106	56-57	0.30763716234606875
1106	58-59	0.3132490896433211
1106	60-61	0.3359659560827808
1106	62-63	0.30808401029445065
1106	64-65	0.27960777155961125
1106	66-67	0.2582047277842676
1106	68-69	0.28876029306485407
1106	70-71	0.2544909012077099
1106	72-73	0.21218766368974684
1106	74-75	0.26788002945777123
1106	76-77	0.24421194150295378
1106	78-79	0.20945380347924214
1106	80-81	0.2655892670887354
1106	82-83	0.33707504475646033
1106	84-85	0.3143082776822794
1106	86-87	0.3152705622604799
1106	88-89	0.25713265179181377
1106	90-91	0.238336959055772
1106	92-93	0.2531142197378742
1106	94-95	0.3207776734261749
1106	96-97	0.311275614039797
1106	98-99	0.2973377552229337
1106	100	0.38495684388400875
1107	1	0.10798523815940086
1107	2	0.08990816712276484
1107	3	0.12361157985409221
1107	4	0.08476169428713831
1107	5	0.06201037284516531
1107	6	0.05949561169623507
1107	7	0.046592076841335484
1107	8	0.07219750863866636
1107	9	0.07047615406030161
1107	10-11	0.10871269672077943
1107	12-13	0.09379131921842543
1107	14-15	0.187001658250729
1107	16-17	0.17050331171918032
1107	18-19	0.15560559721987488
1107	20-21	0.1433565331656652
1107	22-23	0.14382777689300497
1107	24-25	0.1536407295402853
1107	26-27	0.15707404538264313
1107	28-29	0.17589107311675178
1107	30-31	0.20350224453620314
1107	32-33	0.17395768691179825
1107	34-35	0.21610151622470397
1107	36-37	0.210938045345074
1107	38-39	0.22563454904656766
1107	40-41	0.20677775931594056
1107	42-43	0.21354204290056344
1107	44-45	0.21334957062998683
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2216	78-79	-1.7960128436255758
2216	80-81	-1.988670555921086
2216	82-83	-1.9492802484493907
2216	84-85	-2.0356336764584455
2216	86-87	-1.9811618658794785
2216	88-89	-1.9168842902865322
2216	90-91	-2.0693746503808903
2216	92-93	-2.2591310605245454
2216	94-95	-2.2914763814329113
2216	96-97	-2.2452518954414096
2216	98-99	-2.2364574670510535
2216	100	-2.5842843941456195
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	15.0
8	87.0
9	119.0
10	107.0
11	159.0
12	152.0
13	237.0
14	344.0
15	444.0
16	889.0
17	1885.0
18	3579.0
19	5480.0
20	8527.0
21	12690.0
22	18934.0
23	25937.0
24	33935.0
25	43717.0
26	54968.0
27	70415.0
28	87183.0
29	109568.0
30	136491.0
31	171814.0
32	220302.0
33	292164.0
34	417486.0
35	693670.0
36	1511883.0
37	3055206.0
38	2441669.0
39	93282.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	56.9345927728311	15.001089572075166	12.080605417333436	15.983712237760288
2	11.623545245848756	33.00255112320651	25.71231701054276	29.66158662040198
3	45.0533723812446	17.926258227701208	26.723096301327775	10.297273089726419
4	9.625510328502292	11.958494683333544	50.08071704570778	28.33527794245639
5	9.293296849856246	46.393222157644836	33.124676054211214	11.188804938287703
6	23.946705092563054	38.629841241943026	12.69324418121715	24.730209484276774
7	25.002493831118493	34.52952785391248	21.825600004204563	18.642378310764467
8	20.71861190647376	12.741078974255476	18.774728070064793	47.76558104920597
9	13.255391297401161	33.06008947304247	33.13548775001367	20.549031479542712
10-11	29.49593629250285	16.98654961170886	25.785674642717492	27.7318394530708
12-13	43.07732817013333	18.50671363160484	19.175722238339706	19.24023595992213
14-15	25.646593316680466	24.413789449828855	37.60067298219914	12.338944251291537
16-17	17.719483301588905	26.854857528475385	32.78824888474039	22.637410285195323
18-19	14.999169599347454	43.469214209731874	21.88714329561843	19.644472895302243
20-21	24.66750337415961	24.410257865681118	16.41929661911309	34.50294214104618
22-23	17.58332849806463	27.515012458174926	23.079531130300822	31.822127913459624
24-25	32.9304313458377	25.200972725017557	19.22546218628725	22.6431337428575
26-27	27.98432329704798	15.366327568881207	37.59306794148934	19.056281192581473
28-29	16.725975524190687	41.55268710959981	23.531921826672168	18.18941553953734
30-31	22.569835356001448	22.891348058181322	21.22009061787211	33.31872596794512
32-33	25.780071964353983	25.65258756642396	26.422297744394413	22.145042724827636
34-35	34.499166592472704	20.146992338032657	25.13863934198594	20.215201727508692
36-37	26.75312221265035	17.099660053384667	36.80233119464948	19.344886539315496
38-39	20.615761621850346	29.340980997814086	31.893479154231365	18.149778226104207
40-41	20.899676806412387	33.22170760558977	23.79709289225859	22.08152269573925
42-43	17.118017789085783	32.81875834840634	15.59331333876601	34.469910523741866
44-45	25.050733552616368	19.1525651952167	20.084337633080317	35.712363619086624
46-47	33.93732229429156	29.628952031211824	22.344068471925503	14.089657202571118
48-49	28.653350487356565	25.69883820367545	25.441527855219597	20.206283453748387
50-51	10.931004394536844	28.76321963865086	40.38675224838052	19.919023718431774
52-53	23.508828950270157	35.79307189193474	18.663538715335335	22.034560442459775
54-55	16.800410539607938	22.946426872684896	26.683886709316283	33.56927587839088
56-57	28.93341897399703	17.188330326081854	25.936738557066942	27.941512142854176
58-59	41.49624914402087	19.0992101156826	19.62737466045307	19.777166079843454
60-61	25.19588361643518	24.551811768405965	37.26993529691373	12.982369318245127
62-63	17.984409530328666	26.50955349604599	32.49159919675203	23.01443777687332
64-65	15.296816288799473	42.41351984946596	22.08304169492386	20.206622166810707
66-67	24.435528058889492	24.322320680305967	16.89890411326099	34.34324714754355
68-69	17.63841164671732	27.38568687474282	23.34038025633084	31.635521222209018
70-71	32.12730845421517	25.220297709963386	19.46031561328304	23.19207822253841
72-73	27.336087386002184	15.869339371099226	36.9488798026198	19.84569344027879
74-75	16.889924344706923	40.580210672639595	23.532730700710218	18.997134281943264
76-77	22.467551095202605	22.842369169365956	21.472433653274727	33.21764608215671
78-79	25.48003312378343	25.411361609080913	26.443804668906175	22.664800598229487
80-81	33.59585047481078	20.326265673296632	25.14382233030752	20.934061521585072
82-83	26.117178682314666	17.636796276855033	36.12719869343049	20.118826347399803
84-85	20.391406038868464	29.013127805823974	31.410459967765707	19.185006187541855
86-87	20.877572988171046	32.67410709428882	23.87045366643834	22.577866251101792
88-89	17.328514827569062	32.228996366306646	16.137386803814092	34.3051020023102
90-91	24.680240530304424	19.33405749883928	20.505180071349294	35.480521899507
92-93	32.850860042802424	29.31053620757073	22.634214819393115	15.204388930233732
94-95	27.753056715313427	25.689285098617958	25.474479370325053	21.083178815743555
96-97	11.337374872608052	28.43063999517736	39.431538774900325	20.800446357314264
98-99	23.23802197828765	34.877162395978026	19.12183239396876	22.762983231765563
100	20.778295631962614	12.945567427344349	20.216698483182096	46.05943845751094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	72.0
1	47.0
2	45.5
3	85.5
4	68.5
5	40.0
6	73.5
7	158.5
8	251.5
9	288.0
10	231.5
11	165.5
12	168.5
13	208.0
14	252.0
15	303.5
16	362.5
17	426.0
18	518.0
19	597.0
20	687.0
21	819.0
22	990.0
23	1272.5
24	1573.5
25	1942.0
26	2560.5
27	3288.0
28	4205.5
29	5596.5
30	7166.0
31	8641.0
32	10471.0
33	12328.5
34	14053.5
35	16129.0
36	18570.5
37	21197.0
38	22637.5
39	23479.0
40	24558.5
41	25226.0
42	26342.0
43	28509.5
44	32439.0
45	44228.0
46	104822.5
47	958765.5
48	2975550.5
49	3113107.0
50	1296061.5
51	355659.0
52	106229.5
53	47330.5
54	34162.5
55	28085.5
56	24407.5
57	21125.5
58	17838.5
59	16026.0
60	13542.5
61	10073.5
62	7497.5
63	5882.5
64	4273.0
65	2687.5
66	1850.0
67	1326.0
68	996.0
69	775.0
70	572.0
71	410.0
72	310.5
73	280.0
74	227.0
75	139.5
76	86.0
77	61.5
78	36.5
79	21.5
80	8.5
81	5.5
82	4.0
83	2.0
84	1.5
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006002009779807179
2	0.0950125506123942
3	4.0994462594129595E-4
4	0.0
5	0.03980667431896635
6	0.004740641699987807
7	5.255700332580717E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.2045602660645734E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.045472319277489E-4
24-25	0.0
26-27	0.0
28-29	5.255700332580717E-5
30-31	1.5241530964484078E-4
32-33	0.001671312705760668
34-35	0.0013349478844755021
36-37	7.830993495545268E-4
38-39	7.830993495545268E-4
40-41	0.0014873631941203429
42-43	6.569625415725896E-4
44-45	5.465928345883946E-4
46-47	3.521319222829081E-4
48-49	6.884967435680739E-4
50-51	8.093778512174304E-4
52-53	0.0012403452784890492
54-55	0.0015451758977787307
56-57	9.09236157536464E-4
58-59	0.002643617267288101
60-61	0.0018710293183987353
62-63	0.0010931856691767893
64-65	0.004367486976374575
66-67	0.004283395771053284
68-69	0.0036211775291481144
70-71	0.00168707980675841
72-73	0.0049298469119607126
74-75	0.0034897850208335957
76-77	0.003484529320501015
78-79	7.778436492219461E-4
80-81	6.306840399096861E-5
82-83	1.839495116403251E-4
84-85	9.460260598645291E-5
86-87	3.678990232806502E-5
88-89	1.9971661263806727E-4
90-91	1.0511400665161434E-5
92-93	0.0
94-95	0.0
96-97	2.6804071696161653E-4
98-99	4.782687302648453E-4
100	8.093778512174304E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9513480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.00321212523357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78441569945618	35.00548559759974
2	14.395904178451424	12.957238589547712
3	4.337748272819728	5.856378170027152
4	1.7009406778683493	3.061911765541916
5	0.7978807919236146	1.7953599264793885
6	0.4105254148697361	1.1084977396909348
7	0.20152461735366403	0.6348478572256427
8	0.11573750460022185	0.4166847576295184
9	0.07858639014118286	0.31829759871120145
>10	0.17387670592379917	1.0322825382813696
>50	0.0010310716355210517	0.033983184033609906
>100	6.243898772592968E-4	0.05930139106374006
>500	2.7198169317943965E-4	0.08511673963872218
>1k	3.884597447126842E-4	0.3796464692026661
>5k	7.769194879145839E-5	0.2809084927845534
>10k+	4.6615169274875033E-4	36.9740591825421
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1309480	13.764468943015595	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	531557	5.587408603371216	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	333013	3.5004330697074044	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	288658	3.0341998932041694	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	256159	2.692589882987088	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	221813	2.3315653157414533	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	216437	2.275056025765545	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	206527	2.1708880451737955	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	164410	1.7281793833591914	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	115794	1.217157128621703	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	110932	1.1660506985876884	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	89780	0.9437135517181936	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	56910	0.5982038118543372	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	54578	0.5736912255031807	No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	49900	0.5245188931915555	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	37530	0.39449286696350866	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	36859	0.38743971711718533	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	34991	0.36780442067466373	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	29885	0.31413320887834945	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	23225	0.2441272804483743	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	21854	0.229716150136438	No Hit
CAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	14770	0.15525338782443437	No Hit
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	14300	0.1503130295118085	No Hit
GTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	10611	0.11153647245802799	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTAT	9704	0.10200263205472657	No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA	9662	0.10156115322678977	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.0511400665161434E-5	0.0
4	0.0	0.0	0.0	1.0511400665161434E-5	0.0
5	0.0	0.0	0.0	1.0511400665161434E-5	0.0
6	0.0	0.0	0.0	1.0511400665161434E-5	0.0
7	0.0	0.0	0.0	1.0511400665161434E-5	0.0
8	0.0	0.0	0.0	2.1022801330322867E-5	0.0
9	0.0	0.0	0.0	2.3125081463355154E-4	0.0
10-11	0.0	0.0	0.0	7.463094472264618E-4	0.0
12-13	0.0	0.0	0.0	0.0014242947901293742	0.0
14-15	0.0	0.0	0.0	0.0027750097756026187	0.0
16-17	0.0	0.0	0.0	0.005450161244886204	0.0
18-19	0.0	0.0	0.0	0.008929434865054639	0.0
20-21	0.0	0.0	0.0	0.016035141714703768	0.0
22-23	0.0	0.0	0.0	0.025978926743946484	0.0
24-25	0.0	0.0	0.0	0.03416205216177466	0.0
26-27	0.0	0.0	0.0	0.04022187464524023	0.0
28-29	0.0	0.0	0.0	0.05475914176515849	0.0
30-31	0.0	0.0	0.0	0.06923334048108579	0.0
32-33	0.0	0.0	0.0	0.12478083729613137	0.0
34-35	0.0	0.0	0.0	0.18585733086105188	0.0
36-37	0.0	0.0	0.0	0.22531712895806794	0.0
38-39	0.0	0.0	0.0	0.28244659157322033	0.0
40-41	0.0	0.0	0.0	0.3383199418088859	0.0
42-43	0.0	0.0	0.0	0.4106120998835337	0.0
44-45	0.0	0.0	0.0	0.5011047482099085	0.0
46-47	0.0	0.0	0.0	0.6202830089515088	0.0
48-49	0.0	0.0	0.0	0.6856954552908084	0.0
50-51	0.0	0.0	0.0	0.7673900612604431	0.0
52-53	0.0	0.0	0.0	0.8485117958938264	0.0
54-55	0.0	0.0	0.0	0.966144880737648	0.0
56-57	0.0	0.0	0.0	1.178690657887545	0.0
58-59	0.0	0.0	0.0	1.3028040212414385	0.0
60-61	0.0	0.0	0.0	1.4143352379991339	0.0
62-63	0.0	0.0	0.0	1.5248468488923086	0.0
64-65	0.0	0.0	0.0	1.631437707337378	0.0
66-67	0.0	0.0	0.0	1.7864020316435205	0.0
68-69	0.0	0.0	0.0	1.9698522517522505	0.0
70-71	0.0	0.0	0.0	2.1226775060230327	0.0
72-73	0.0	0.0	0.0	2.231975050139381	0.0
74-75	0.0	0.0	0.0	2.397613701820995	0.0
76-77	0.0	0.0	0.0	2.5187365716856505	0.0
78-79	0.0	0.0	0.0	2.8337264597182106	0.0
80-81	0.0	0.0	0.0	3.12606953501768	0.0
82-83	0.0	0.0	0.0	3.2745535808137505	0.0
84-85	0.0	0.0	0.0	3.4526482422835807	0.0
86-87	0.0	0.0	0.0	3.62155593957206	0.0
88	0.0	0.0	0.0	3.785386630339266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	27210	0.0	36.250835	1
GTACGCA	27395	0.0	35.869736	3
AGTACGC	27500	0.0	35.729958	2
TACGCAG	27485	0.0	35.718067	4
CGCAGTG	28390	0.0	34.69882	6
ACGCAGT	29125	0.0	33.771393	5
GCTTATG	2620	0.0	28.739155	1
AGCTTAC	1840	0.0	28.64544	1
GACGTCC	85	6.822478E-4	27.645176	7
GTACCGT	90	9.5239433E-4	26.110567	6
TTATGTA	2945	0.0	25.86324	3
AGTAAGG	970	0.0	25.19469	94
TATGTAC	3085	0.0	24.994354	4
GTAGGCA	9945	0.0	24.536678	3
TAGGCAG	9970	0.0	24.333677	4
ACGCCTA	195	1.760418E-8	24.102062	6
AGTAGGC	10220	0.0	23.759295	2
GAGTAGG	10710	0.0	23.156687	1
AGGCAGT	10695	0.0	23.079782	5
GGCAGTG	10765	0.0	22.921028	6
>>END_MODULE