FastQCFastQC Report
Mon 16 Jan 2017
ERR1146526_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146526_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5512116
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT546630.991688128479154No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA297250.5392665901806131No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG222300.4032933994857873No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT159440.28925370946475No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC112370.20386000584893352No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG69580.12623101545758472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG28850.025.2584931
TAGTACT39750.023.170094
CTAGTAC43450.020.5481433
TAGGCAT43350.020.1615895
GCATCGT34100.020.1183098
CATGGGG97550.020.0871144
CTAACGC13300.019.7854143
CTGTGCG43050.019.7561559
GTGATCG18950.019.3409778
TGATCGC19000.019.2902559
TCTAACG14800.019.04982
CGATAAT22050.018.33429166-67
TAACGCC15750.017.9010894
GTACTGT51600.017.848086
TTTGCGT10800.017.8389343
ATACGGG19800.017.799383
TACTAGG40200.017.7671782
GTATATA12450.017.7480281
GGGCACC44000.017.73445794
ATGGGGG68350.017.668335
Produced by FastQC (version 0.11.5)