##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146526_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5512116
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51755006607263	31.0	31.0	34.0	30.0	34.0
2	31.84850572810877	33.0	31.0	34.0	30.0	34.0
3	31.999649136556634	33.0	31.0	34.0	30.0	34.0
4	35.580200235263554	37.0	35.0	37.0	33.0	37.0
5	35.51785666339388	37.0	35.0	37.0	33.0	37.0
6	35.68181602128838	37.0	35.0	37.0	35.0	37.0
7	35.675437164239646	37.0	35.0	37.0	35.0	37.0
8	35.66664054239787	37.0	35.0	37.0	35.0	37.0
9	37.42742043164549	39.0	38.0	39.0	35.0	39.0
10-11	37.40947959005217	39.0	38.0	39.0	35.0	39.0
12-13	37.381437818072044	39.0	38.0	39.0	35.0	39.0
14-15	38.79012052721677	40.0	39.0	41.0	36.0	41.0
16-17	38.78749748735331	40.0	39.0	41.0	35.0	41.0
18-19	38.8124993196805	40.0	39.0	41.0	35.0	41.0
20-21	38.857440863000704	40.5	39.0	41.0	35.0	41.0
22-23	38.836775568583825	40.5	39.0	41.0	35.0	41.0
24-25	38.834255755865804	40.5	39.0	41.0	35.0	41.0
26-27	38.7814307608911	40.0	39.0	41.0	35.0	41.0
28-29	38.7004706541009	40.0	39.0	41.0	35.0	41.0
30-31	38.69091778910313	40.0	39.0	41.0	35.0	41.0
32-33	38.67112865549274	40.0	38.0	41.0	35.0	41.0
34-35	38.58817348909203	40.0	38.0	41.0	34.0	41.0
36-37	38.47305499376283	40.0	38.0	41.0	34.0	41.0
38-39	38.39884909896671	40.0	38.0	41.0	34.0	41.0
40-41	38.33616582089346	40.0	38.0	41.0	33.5	41.0
42-43	38.198524305366576	40.0	38.0	41.0	33.0	41.0
44-45	38.04500830534045	40.0	38.0	41.0	33.0	41.0
46-47	37.89613834324241	40.0	37.5	41.0	33.0	41.0
48-49	37.73009403285417	40.0	37.0	41.0	33.0	41.0
50-51	37.55015297210726	40.0	37.0	41.0	32.0	41.0
52-53	37.392050892978304	40.0	36.0	41.0	32.0	41.0
54-55	37.18067843637543	39.0	36.0	41.0	31.0	41.0
56-57	36.9531756951414	39.0	35.0	41.0	31.0	41.0
58-59	36.68893334247683	39.0	35.0	40.5	31.0	41.0
60-61	36.413580193159945	38.0	35.0	40.0	31.0	41.0
62-63	36.12362112843779	37.5	35.0	40.0	30.0	41.0
64-65	35.80233616273678	37.0	35.0	40.0	30.0	41.0
66-67	35.45730260393649	36.5	35.0	39.0	29.5	41.0
68-69	35.09632562159432	36.0	34.0	39.0	29.0	41.0
70-71	34.70258644774529	35.5	34.0	38.5	29.0	40.0
72-73	34.338747225203534	35.0	34.0	37.0	29.0	39.0
74-75	34.01496394488069	35.0	34.0	37.0	29.0	39.0
76-77	33.67080754468883	35.0	34.0	36.0	28.0	39.0
78-79	33.38973544823803	35.0	33.5	36.0	28.0	37.0
80-81	33.13042495840073	35.0	33.0	35.5	27.0	37.0
82-83	32.91078952257173	35.0	33.0	35.0	27.0	36.5
84-85	32.756584948502535	35.0	33.0	35.0	27.0	36.0
86-87	32.639246978837164	35.0	33.0	35.0	27.0	36.0
88-89	32.49949601931454	35.0	33.0	35.0	26.0	36.0
90-91	32.33666327051172	35.0	33.0	35.0	25.5	35.0
92-93	32.24961203646657	35.0	33.0	35.0	25.0	35.0
94-95	32.170700689172726	35.0	33.0	35.0	25.0	35.0
96-97	32.09706472069891	35.0	33.0	35.0	25.0	35.0
98-99	32.05256493150725	35.0	33.0	35.0	25.0	35.0
100	30.920347467288423	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15670640812803782
1101	2	0.19485675393706714
1101	3	0.2483581781428228
1101	4	0.22481755059875752
1101	5	0.16114570140862838
1101	6	0.20462451117486324
1101	7	0.20182738598050776
1101	8	0.21299751090769092
1101	9	0.351836171389472
1101	10-11	0.2991477316383069
1101	12-13	0.3101937614009671
1101	14-15	0.39887527555838176
1101	16-17	0.40273082884996825
1101	18-19	0.3460569033938441
1101	20-21	0.39744199975240235
1101	22-23	0.38982475245936854
1101	24-25	0.37178391923248455
1101	26-27	0.42169975781391855
1101	28-29	0.3987771186259863
1101	30-31	0.38104496902524687
1101	32-33	0.38391111509011466
1101	34-35	0.37982456124578334
1101	36-37	0.39419946712641973
1101	38-39	0.4204196382235139
1101	40-41	0.38606178206090647
1101	42-43	0.41023446950084974
1101	44-45	0.3448313129312979
1101	46-47	0.3600793731477978
1101	48-49	0.35536169650085725
1101	50-51	0.3869552103759375
1101	52-53	0.3753828207461609
1101	54-55	0.40811453802910336
1101	56-57	0.3650296563003721
1101	58-59	0.400422658472138
1101	60-61	0.4168665249546635
1101	62-63	0.442738637905542
1101	64-65	0.371242457061868
1101	66-67	0.3738181377762757
1101	68-69	0.3519060708224728
1101	70-71	0.3372971510516436
1101	72-73	0.31114691177331366
1101	74-75	0.3373107200058527
1101	76-77	0.27120610624677965
1101	78-79	0.2956300512197174
1101	80-81	0.3236179156342587
1101	82-83	0.319452109861885
1101	84-85	0.328446740368328
1101	86-87	0.31245674880355523
1101	88-89	0.2918086064873009
1101	90-91	0.33991715793990096
1101	92-93	0.37866866182189796
1101	94-95	0.39244238712670665
1101	96-97	0.315838843738355
1101	98-99	0.2886893435462241
1101	100	0.20859942453782665
1102	1	0.09322448172043707
1102	2	0.16095949942746302
1102	3	0.1706071466725163
1102	4	0.1378092331044769
1102	5	0.11330281147894539
1102	6	0.15505979406722759
1102	7	0.17911139878460602
1102	8	0.23760420987750308
1102	9	0.29629556885720376
1102	10-11	0.30351353394333813
1102	12-13	0.2769002592314749
1102	14-15	0.37181392634055044
1102	16-17	0.3600701993607487
1102	18-19	0.3229831391689615
1102	20-21	0.3361763593321001
1102	22-23	0.3313657445390277
1102	24-25	0.3277811501939638
1102	26-27	0.3343954165860765
1102	28-29	0.38872790857229234
1102	30-31	0.3756295913361001
1102	32-33	0.36445081236865917
1102	34-35	0.3451716216851324
1102	36-37	0.3562432163667637
1102	38-39	0.3107372807998203
1102	40-41	0.39478473775006506
1102	42-43	0.35759318707113863
1102	44-45	0.33622214821179597
1102	46-47	0.37079126352283964
1102	48-49	0.36674555037237866
1102	50-51	0.3603962769747042
1102	52-53	0.2970073083552549
1102	54-55	0.34537486201537604
1102	56-57	0.3332164457771043
1102	58-59	0.40495976707098436
1102	60-61	0.3535205595840196
1102	62-63	0.35528126843678365
1102	64-65	0.2625983737656057
1102	66-67	0.23159683887441673
1102	68-69	0.2705296711675942
1102	70-71	0.21238459164220558
1102	72-73	0.2637849595065873
1102	74-75	0.20653220295674402
1102	76-77	0.2546741263427208
1102	78-79	0.24423626031465062
1102	80-81	0.25630898586557294
1102	82-83	0.3053121620183319
1102	84-85	0.32241313781138814
1102	86-87	0.24511077282356553
1102	88-89	0.24736710651993832
1102	90-91	0.30758060121139863
1102	92-93	0.3037371497437462
1102	94-95	0.1859336589816678
1102	96-97	0.280658291171477
1102	98-99	0.13879036870858386
1102	100	0.00939557500690924
1103	1	0.06408710897227365
1103	2	0.12845290478524873
1103	3	0.160964124428439
1103	4	0.1157981237649679
1103	5	0.0816443751938607
1103	6	0.14797906986916587
1103	7	0.12308255577413973
1103	8	0.16190565641768728
1103	9	0.23068838740677222
1103	10-11	0.26637993271786087
1103	12-13	0.21320311914865897
1103	14-15	0.2894848770348233
1103	16-17	0.21727864386346596
1103	18-19	0.16171462482245857
1103	20-21	0.17598250993868447
1103	22-23	0.1948165726229334
1103	24-25	0.197145911246686
1103	26-27	0.19923336864699337
1103	28-29	0.22116453293487837
1103	30-31	0.2682501718307648
1103	32-33	0.2151700137634549
1103	34-35	0.24846288694546814
1103	36-37	0.24416758464945332
1103	38-39	0.2259359640142904
1103	40-41	0.2174435819433782
1103	42-43	0.17198746695358125
1103	44-45	0.16116429670530152
1103	46-47	0.1662495674383493
1103	48-49	0.13495716119073364
1103	50-51	0.16798089669161698
1103	52-53	0.12747394649383637
1103	54-55	0.1582478075234306
1103	56-57	0.1692325332773379
1103	58-59	0.24151291328384872
1103	60-61	0.17668145846988637
1103	62-63	0.21476557841343435
1103	64-65	0.17460812278168447
1103	66-67	0.1921465813522545
1103	68-69	0.16821533714409043
1103	70-71	0.18084009778495158
1103	72-73	0.16495497926196379
1103	74-75	0.13748984138995723
1103	76-77	0.14621030910523558
1103	78-79	0.13111004542829363
1103	80-81	0.1931369438445074
1103	82-83	0.1769684686089974
1103	84-85	0.17714794601474892
1103	86-87	0.11801224264311827
1103	88-89	0.1160883902196872
1103	90-91	0.14348657897856043
1103	92-93	0.11396790534440981
1103	94-95	0.09999085052832868
1103	96-97	0.14594085470429263
1103	98-99	0.08226994011513256
1103	100	0.01918331451847166
1104	1	0.04547645282285018
1104	2	0.10761754694923198
1104	3	0.11615775669762485
1104	4	0.13888175465483954
1104	5	0.11164692504123508
1104	6	0.1502752350878609
1104	7	0.08415049038170963
1104	8	0.17298750009473451
1104	9	0.20150846221775964
1104	10-11	0.20731120157294924
1104	12-13	0.18148644377134815
1104	14-15	0.22425966520032148
1104	16-17	0.2462962549401908
1104	18-19	0.1803464880649841
1104	20-21	0.19367736226255516
1104	22-23	0.22107623558985523
1104	24-25	0.21802915232574094
1104	26-27	0.22635274415029727
1104	28-29	0.20799118988512078
1104	30-31	0.20594834484459312
1104	32-33	0.1943945389770576
1104	34-35	0.1808292827049769
1104	36-37	0.19115060130982897
1104	38-39	0.24003807424941925
1104	40-41	0.18652316733712837
1104	42-43	0.1297553476913862
1104	44-45	0.14984342937177786
1104	46-47	0.10540664927810184
1104	48-49	0.16949009749254884
1104	50-51	0.16574548885788687
1104	52-53	0.1548228974787733
1104	54-55	0.22078587456781662
1104	56-57	0.1751939301226315
1104	58-59	0.1631981429226812
1104	60-61	0.14844875072647312
1104	62-63	0.16602699451195235
1104	64-65	0.19925499390126333
1104	66-67	0.20142972985882324
1104	68-69	0.15956319110582484
1104	70-71	0.20492049773805832
1104	72-73	0.18796050880852277
1104	74-75	0.12736677779739836
1104	76-77	0.1438351682078789
1104	78-79	0.16507889268137177
1104	80-81	0.11851499128237464
1104	82-83	0.16941096366230823
1104	84-85	0.1996445677792451
1104	86-87	0.13573247745748063
1104	88-89	0.16437574570677072
1104	90-91	0.20992904405466106
1104	92-93	0.20642442545046435
1104	94-95	0.11166542219768871
1104	96-97	0.12089557216282287
1104	98-99	0.10158880789724378
1104	100	0.11268507576889064
1105	1	0.023471557297416012
1105	2	0.06732960687090994
1105	3	0.11645422286861873
1105	4	0.13636266653447393
1105	5	0.02387027302334843
1105	6	0.06869913470016087
1105	7	0.05968263219409664
1105	8	0.15621854822740744
1105	9	0.1328991417800296
1105	10-11	0.19285922396435495
1105	12-13	0.15821310952803458
1105	14-15	0.2612908070278763
1105	16-17	0.21908493290519004
1105	18-19	0.13796346937554915
1105	20-21	0.20722612857689882
1105	22-23	0.19128151991776576
1105	24-25	0.20213435699181304
1105	26-27	0.20135871399568828
1105	28-29	0.19075342734518586
1105	30-31	0.1939493591382231
1105	32-33	0.16302945383433354
1105	34-35	0.1615956363234048
1105	36-37	0.17718492944251807
1105	38-39	0.2020456536411359
1105	40-41	0.1588421939246203
1105	42-43	0.19523446322007487
1105	44-45	0.149106576760353
1105	46-47	0.11946800176848171
1105	48-49	0.12364337467285225
1105	50-51	0.1043516189887228
1105	52-53	0.14901535016164047
1105	54-55	0.16422227462107486
1105	56-57	0.12481865795703584
1105	58-59	0.1326085575949847
1105	60-61	0.16784773853795798
1105	62-63	0.11448869961412811
1105	64-65	0.13088969646373982
1105	66-67	0.11567323283261999
1105	68-69	0.12643868718475204
1105	70-71	0.15324562192445512
1105	72-73	0.13363984224118752
1105	74-75	0.15131270117064588
1105	76-77	0.140980975119966
1105	78-79	0.12639240293331966
1105	80-81	0.13633736318594458
1105	82-83	0.12181506184978019
1105	84-85	0.14586281826497327
1105	86-87	0.10149750326661433
1105	88-89	0.10554551290987746
1105	90-91	0.1291212386411189
1105	92-93	0.13400938532075912
1105	94-95	0.09640977275333285
1105	96-97	0.10093404349097312
1105	98-99	0.0628922717258007
1105	100	0.09567057081846642
1106	1	4.0951748158235546E-4
1106	2	0.031086471116612557
1106	3	0.03995613342765836
1106	4	0.0365603274481785
1106	5	0.025915771266156185
1106	6	0.02247909342253962
1106	7	0.028331062132636475
1106	8	0.05419484918295581
1106	9	0.07494831473588448
1106	10-11	0.05526760278691967
1106	12-13	0.09506044132528757
1106	14-15	0.05532973639957106
1106	16-17	0.050763578778763474
1106	18-19	0.08851512179023047
1106	20-21	0.07467522302552254
1106	22-23	0.026182786238187816
1106	24-25	0.03009833194214906
1106	26-27	0.055385333988418495
1106	28-29	0.10164163598028608
1106	30-31	0.06966962438387014
1106	32-33	0.06629979316242895
1106	34-35	0.053479455034768364
1106	36-37	0.06442001185331492
1106	38-39	0.051301569736210695
1106	40-41	0.03197181627460566
1106	42-43	-0.03928798046473503
1106	44-45	-0.021314052088634128
1106	46-47	-0.02264619233924492
1106	48-49	-0.0023335071972994115
1106	50-51	0.007867179691920967
1106	52-53	-0.0023397089008980743
1106	54-55	0.012896734979548796
1106	56-57	-0.04035990520883814
1106	58-59	0.027194869888063522
1106	60-61	0.0037357409135694297
1106	62-63	0.02274220581394104
1106	64-65	0.05226207567794461
1106	66-67	0.02664123174118771
1106	68-69	0.025651094288377863
1106	70-71	0.06264150775788835
1106	72-73	0.06158429233750695
1106	74-75	0.03148523857883134
1106	76-77	0.011062764306814188
1106	78-79	0.009425947881055663
1106	80-81	0.05903813987296758
1106	82-83	0.03414723602038805
1106	84-85	0.07702093259698728
1106	86-87	-0.004278558076563854
1106	88-89	-2.3991245792132077E-4
1106	90-91	0.04344028786856313
1106	92-93	0.024513814190704863
1106	94-95	0.030928575918679257
1106	96-97	-0.02554341372555058
1106	98-99	-0.006280891801530686
1106	100	-0.13861550574901216
1107	1	-0.02703270797479007
1107	2	-7.510587163714888E-4
1107	3	0.014415682294881549
1107	4	0.020022868224039314
1107	5	0.02480533628194337
1107	6	-0.005597096496877896
1107	7	-0.056901765915327474
1107	8	-0.007668419940614513
1107	9	0.024520099989395305
1107	10-11	0.054705405077584146
1107	12-13	0.018840911796694115
1107	14-15	0.019101776435476836
1107	16-17	-0.00823620877854836
1107	18-19	-0.026813969555448125
1107	20-21	0.03632744252772824
1107	22-23	0.03749594726959771
1107	24-25	0.028752229048038203
1107	26-27	0.011323702450603434
1107	28-29	-8.630624202936588E-4
1107	30-31	0.050850385726427305
1107	32-33	0.0447478515502695
1107	34-35	0.01708993057567909
1107	36-37	0.0432755422824016
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2216	40-41	-0.24266100049898398
2216	42-43	-0.2410359742087138
2216	44-45	-0.2328599022987845
2216	46-47	-0.16259894581233425
2216	48-49	-0.17607254192103738
2216	50-51	-0.22026307560841474
2216	52-53	-0.17868148471485057
2216	54-55	-0.1991751607983474
2216	56-57	-0.24235570720526312
2216	58-59	-0.2702373371776403
2216	60-61	-0.3725820638402837
2216	62-63	-0.3701926083937437
2216	64-65	-0.38553714763154545
2216	66-67	-0.3548317717954248
2216	68-69	-0.3324168548652082
2216	70-71	-0.41735039006402985
2216	72-73	-0.3416114533475465
2216	74-75	-0.29075331893672995
2216	76-77	-0.31912358780079586
2216	78-79	-0.3790753015714756
2216	80-81	-0.380233359693392
2216	82-83	-0.32765272452745364
2216	84-85	-0.3658913540449831
2216	86-87	-0.2798693999446158
2216	88-89	-0.3312685334812002
2216	90-91	-0.3248818656761827
2216	92-93	-0.28217597001341943
2216	94-95	-0.3594747665906546
2216	96-97	-0.285785911729306
2216	98-99	-0.35110142269764566
2216	100	-0.370926286771013
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	150.0
1	86.0
2	89.0
3	76.0
4	61.0
5	64.0
6	135.0
7	575.0
8	1942.0
9	2560.0
10	2706.0
11	3284.0
12	4505.0
13	6166.0
14	8767.0
15	10270.0
16	10674.0
17	11358.0
18	12331.0
19	13476.0
20	14767.0
21	16660.0
22	18429.0
23	21593.0
24	26140.0
25	31951.0
26	37050.0
27	40968.0
28	48421.0
29	59540.0
30	73710.0
31	92319.0
32	121051.0
33	167033.0
34	256987.0
35	415412.0
36	802253.0
37	1537305.0
38	1428192.0
39	212969.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.56601236286377	17.10233671631127	11.931859025885336	24.39979189493962
2	15.04714644700674	20.813491667498255	38.00498952200379	26.134372363491217
3	22.28281942338471	24.547273407180956	27.783070809637916	25.386836359796416
4	12.834642583432036	15.927201630321186	38.258044195174065	32.98011159107271
5	14.080910923303055	38.14792524393445	33.58554303347287	14.18562079928962
6	31.21596170873674	35.09365973320947	18.517447345884197	15.172931212169596
7	28.135476678377596	31.587960329792843	21.217573832830336	19.05898915899923
8	25.436010014877176	31.794422874560034	20.851972132515694	21.9175949780471
9	26.305525165602567	17.35242729543933	20.099624616043354	36.242422922914756
10-11	25.60616883299493	24.342014189070646	26.930177892257333	23.12163908567709
12-13	28.828499965709646	21.794069546645222	25.73898435960676	23.63844612803837
14-15	25.616565673609852	23.06476392391025	25.315995095724542	26.00267530675535
16-17	23.549821329590888	26.04759645435173	26.535399783545266	23.86718243251212
18-19	23.130919099773852	27.969044531869216	26.182658779991762	22.717377588365174
20-21	24.687426775200468	26.408127352888854	24.47936969090437	24.425076181006304
22-23	24.090832027313613	25.861793859244504	24.02473958297899	26.02263453046289
24-25	25.074312105035517	25.96606643676359	24.238573062442487	24.721048395758405
26-27	25.51670635265853	24.54697961767735	25.628992506182858	24.30732152348126
28-29	24.01514798962835	26.390598492730398	25.61515575485084	23.979097762790413
30-31	24.337439678278102	25.984930852562123	25.25534135551567	24.422288113644104
32-33	24.099183729233754	26.372518406416805	25.06441497450163	24.463882889847806
34-35	24.94672217666546	26.002852793721132	25.001099984858765	24.049325044754642
36-37	25.22886562994571	24.790168703346378	25.574414081850172	24.406551584857745
38-39	25.31717794889824	25.120700409403813	25.588595665536364	23.973525976161582
40-41	24.629246360780623	25.370635694833965	26.131331617514704	23.868786326870705
42-43	24.32041501406432	25.735212592544066	24.780633427047082	25.16373896634453
44-45	24.571921133099238	25.400885574035257	25.046592445400183	24.98060084746532
46-47	24.815683103330972	26.061669226516443	24.509462945119644	24.613184725032937
48-49	25.03954386810532	25.562987447589403	24.9122920997921	24.485176584513177
50-51	23.83315179497938	25.50281163650565	26.075582988555645	24.588453579959328
52-53	24.21946547863574	26.210970098122527	24.825437107044642	24.74412731619709
54-55	23.72841536580222	25.130134840783775	25.56621042189423	25.57523937151977
56-57	24.848505747335047	25.055349917285923	24.979378197060043	25.116766138318987
58-59	24.952881749479545	25.195809565499406	25.115175425080576	24.736133259940477
60-61	24.509173251721933	25.134507359281905	26.189686070699086	24.166633318297077
62-63	24.67082246993872	25.14758533880226	25.387191754911477	24.794400436347548
64-65	23.609809223360262	26.11692289934904	25.5798242555889	24.693443621701796
66-67	24.65903586491343	24.744606978138258	25.174876321157193	25.421480835791122
68-69	24.330746174389574	25.321734222277883	25.19996261278552	25.147556990547027
70-71	24.632938850395643	25.321289102023936	24.659926572343338	25.385845475237083
72-73	24.656954367839433	24.332569830060176	25.80332540429649	25.207150397803897
74-75	24.148958045408868	26.438875197573275	24.993570758229474	24.41859599878838
76-77	24.565250118489573	24.643344797080424	24.821984332606743	25.969420751823257
78-79	24.535822124622563	25.04352284353782	25.32430133188749	25.096353699952132
80-81	24.75481250282453	25.37246157411852	25.424823172974953	24.44790275008199
82-83	24.546230080571092	24.642945521475266	25.990600507787825	24.820223890165817
84-85	24.261412512839872	25.20032013473244	25.465266107459282	25.073001244968403
86-87	24.214401089746865	25.579016492928975	25.611455916728588	24.595126500595573
88-89	24.622497878783562	24.883894534325506	25.052956686715728	25.440650900175203
90-91	24.68976616247562	24.195716768606452	25.15448854767608	25.960028521241846
92-93	24.957043083228616	25.361177473225883	25.318275001215934	24.363504442329567
94-95	24.422662726538107	25.231568760098167	25.715681884074737	24.630086629288986
96-97	23.69103562571027	24.65085874755954	26.131512745193763	25.52659288153643
98-99	24.3463823368433	25.459485032687233	24.97760601392855	25.216526616540918
100	24.185214785069753	24.606626002716254	24.762563759724106	26.445595452489883
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	330.0
1	247.5
2	163.5
3	174.0
4	177.5
5	147.0
6	129.0
7	140.5
8	147.0
9	155.5
10	158.0
11	149.5
12	161.5
13	186.0
14	214.0
15	229.0
16	249.0
17	275.5
18	336.5
19	431.0
20	539.5
21	675.0
22	843.5
23	1167.0
24	1619.0
25	2097.0
26	2780.5
27	3722.0
28	4778.0
29	6221.0
30	8011.5
31	10078.0
32	12799.0
33	15979.0
34	20315.5
35	26075.5
36	34518.0
37	47465.0
38	63019.5
39	81654.0
40	100462.5
41	117125.5
42	134477.5
43	159594.5
44	200192.5
45	237874.0
46	257015.0
47	310053.5
48	468215.5
49	480962.0
50	365713.5
51	320388.0
52	273021.5
53	246571.5
54	228797.0
55	206194.0
56	185935.5
57	160774.0
58	137253.5
59	125307.5
60	106472.5
61	83660.0
62	66009.0
63	52195.0
64	38626.5
65	25791.0
66	18641.5
67	14489.0
68	10930.0
69	8173.0
70	6227.0
71	4518.0
72	3332.5
73	2644.0
74	1893.0
75	1297.0
76	908.0
77	588.0
78	353.0
79	229.5
80	169.0
81	131.5
82	101.0
83	83.0
84	76.0
85	54.5
86	32.5
87	25.0
88	19.5
89	15.5
90	14.0
91	15.0
92	12.0
93	8.0
94	8.0
95	6.5
96	7.5
97	5.5
98	4.0
99	7.5
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06095662718273708
2	0.010268288983758688
3	0.012282034703188394
4	0.010232005277102297
5	0.012644871769752306
6	0.012263892849860198
7	0.0031022569191214407
8	0.005732825651709798
9	0.007728429517811309
10-11	0.0074200180112319845
12-13	0.006567350904806793
14-15	0.010857899216925043
16-17	0.010358998250399664
18-19	0.008281756044321274
20-21	0.007392805231239691
22-23	0.0025307885392832808
24-25	0.004136342558828588
26-27	0.005542336191763744
28-29	0.0065582799781426955
30-31	0.009497260217310377
32-33	0.010649267903650794
34-35	0.012272963776524296
36-37	0.018540974101415864
38-39	0.019112442481254023
40-41	0.018940094874636167
42-43	0.024727346086330548
44-45	0.028410142311954246
46-47	0.030868363437924744
48-49	0.0322924989241881
50-51	0.0326916196974084
52-53	0.03101349826455031
54-55	0.03740850156273925
56-57	0.039385963575512564
58-59	0.03706380634950353
60-61	0.03812510476920297
62-63	0.03319052066393378
64-65	0.035467323256622316
66-67	0.03771691306931857
68-69	0.04011163770864039
70-71	0.03996650288201482
72-73	0.036084146269780966
74-75	0.03873285685569752
76-77	0.03877821148901801
78-79	0.03770784214265448
80-81	0.042687780881244156
82-83	0.03877821148901801
84-85	0.03512262804338661
86-87	0.03348079031718491
88-89	0.03114049123784768
90-91	0.03332658456389524
92-93	0.035267762870012175
94-95	0.03888706260898718
96-97	0.03414296796366405
98-99	0.030986285484558018
100	0.028736695671861767
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5512116.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.861703955872706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.23284816271959	38.905842836299065
2	13.85577953952728	14.925917912717132
3	5.560597699022194	8.985098012473214
4	2.8219073536689856	6.079709538968765
5	1.6634267936877278	4.479750075693747
6	1.0160210522326016	3.2834775076972007
7	0.6736282536507685	2.5397935902114455
8	0.45673776074953104	1.968053924396757
9	0.3258247683938443	1.5794529495048029
>10	1.3684953043222159	11.559602907269767
>50	0.020765886434642247	0.724405898940689
>100	0.0034225707434757005	0.2840352369232939
>500	1.2107885491557375E-4	0.04623753028083439
>1k	6.053942745633598E-5	0.04887874322247489
>5k	6.053942745633598E-5	0.22688345252967332
>10k+	3.0269713728167983E-4	4.362859882871193
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	54663	0.991688128479154	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	29725	0.5392665901806131	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	22230	0.4032933994857873	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	15944	0.28925370946475	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	11237	0.20386000584893352	No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG	6958	0.12623101545758472	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.814185332819556E-5	0.0
10-11	0.0	0.0	0.0	1.814185332819556E-5	0.0
12-13	0.0	0.0	0.0	3.628370665639112E-5	0.0
14-15	0.0	0.0	0.0	7.256741331278225E-5	0.0
16-17	0.0	0.0	0.0	1.360638999614667E-4	0.0
18-19	0.0	0.0	0.0	2.4491501993064005E-4	0.0
20-21	0.0	0.0	0.0	5.170428198535735E-4	0.0
22-23	0.0	0.0	0.0	9.615182263943647E-4	0.0
24-25	0.0	0.0	0.0	0.001569270312888916	0.0
26-27	0.0	0.0	0.0	0.0027847744858780187	0.0
28-29	0.0	0.0	0.0	0.0068757624113861176	0.0
30-31	0.0	0.0	0.0	0.019729265494412673	0.0
32-33	0.0	0.0	0.0	0.05184941681198291	0.0
34-35	0.0	0.0	0.0	0.09955342013847314	0.0
36-37	0.0	0.0	0.0	0.16854688834560083	0.0
38-39	0.0	0.0	0.0	0.2705948133167009	0.0
40-41	0.0	0.0	0.0	0.3862763410639399	0.0
42-43	0.0	0.0	0.0	0.49679651153930726	0.0
44-45	0.0	0.0	0.0	0.6210137812774622	0.0
46-47	0.0	0.0	0.0	0.7709471281083344	0.0
48-49	0.0	0.0	0.0	0.9260236903577501	0.0
50-51	0.0	0.0	0.0	1.0894001505048152	0.0
52-53	0.0	0.0	0.0	1.2522142131987062	0.0
54-55	0.0	0.0	0.0	1.4179491142784368	0.0
56-57	0.0	0.0	0.0	1.6080122406712776	0.0
58-59	0.0	0.0	0.0	1.8183942427916975	0.0
60-61	0.0	0.0	0.0	2.0408496483020313	1.814185332819556E-5
62-63	0.0	0.0	0.0	2.2687748225908164	1.814185332819556E-5
64-65	0.0	0.0	0.0	2.4985686077724054	1.814185332819556E-5
66-67	0.0	0.0	0.0	2.742240910750064	1.814185332819556E-5
68-69	0.0	0.0	0.0	3.0072933878750012	1.814185332819556E-5
70-71	0.0	0.0	0.0	3.301209553645098	1.814185332819556E-5
72-73	0.0	0.0	0.0	3.5840047633250096	1.814185332819556E-5
74-75	0.0	1.814185332819556E-5	0.0	3.862718056006078	1.814185332819556E-5
76-77	0.0	1.814185332819556E-5	0.0	4.152606730337315	1.814185332819556E-5
78-79	0.0	1.814185332819556E-5	0.0	4.48716064756257	1.814185332819556E-5
80-81	0.0	1.814185332819556E-5	0.0	4.840092262209286	1.814185332819556E-5
82-83	0.0	1.814185332819556E-5	0.0	5.189649492136958	1.814185332819556E-5
84-85	0.0	1.814185332819556E-5	0.0	5.559362321112255	1.814185332819556E-5
86-87	9.07092666409778E-6	1.814185332819556E-5	0.0	5.934399784039378	1.814185332819556E-5
88	1.814185332819556E-5	1.814185332819556E-5	0.0	6.22494156509043	1.814185332819556E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2885	0.0	25.258493	1
TAGTACT	3975	0.0	23.17009	4
CTAGTAC	4345	0.0	20.548143	3
TAGGCAT	4335	0.0	20.161589	5
GCATCGT	3410	0.0	20.118309	8
CATGGGG	9755	0.0	20.087114	4
CTAACGC	1330	0.0	19.785414	3
CTGTGCG	4305	0.0	19.756155	9
GTGATCG	1895	0.0	19.340977	8
TGATCGC	1900	0.0	19.290255	9
TCTAACG	1480	0.0	19.0498	2
CGATAAT	2205	0.0	18.334291	66-67
TAACGCC	1575	0.0	17.901089	4
GTACTGT	5160	0.0	17.84808	6
TTTGCGT	1080	0.0	17.838934	3
ATACGGG	1980	0.0	17.79938	3
TACTAGG	4020	0.0	17.767178	2
GTATATA	1245	0.0	17.748028	1
GGGCACC	4400	0.0	17.734457	94
ATGGGGG	6835	0.0	17.66833	5
>>END_MODULE