FastQCFastQC Report
Mon 16 Jan 2017
ERR1146526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5512116
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG218090.395655679234617No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA206420.37448413640061273No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA185710.33691235815791976No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT165680.30057422594154404No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA92550.1679028525524499No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55360.10043330002489062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG30200.028.8235971
TAGGCAT42950.021.7840295
TAGTACT43300.021.0650334
CTAACGC12550.020.9793783
CTAGTAC46450.020.2438263
GAGTACT75600.020.14161112-13
CGACGAG18600.019.83497424-25
TCTAACG13850.019.7036132
GCATCGT38650.019.5770558
CTGTGCG45350.019.1719119
GGGCACC44600.018.96778194
CTAGGCA54100.018.7717514
TACTAGG44350.018.4596422
CATGGGG97300.018.2653184
GTGATCG16950.018.0224848
TGCGACG20400.017.9697222-23
GTACTTT84100.017.91030714-15
CCTAGTA51700.017.6554532
CAATGCG21600.017.6240718-19
GTTAATA24750.017.4902521
Produced by FastQC (version 0.11.5)