FastQCFastQC Report
Mon 16 Jan 2017
ERR1146525_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146525_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868580
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41740.14550753334402386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG96650.026.32291
TACATGG96100.025.340982
ACATGGG94350.024.615133
CATGGGG67750.021.4416714
GTACTAG9850.019.5742871
GTATCAA97650.018.6370471
GGTATCA78800.018.3807331
ATGGGGG43050.018.3452175
TAGTACT14650.017.6495864
CTAGTAC11200.017.6298373
CAACGCA101700.017.2414825
GAGTACA72950.016.9538691
GTATTAG8600.016.9512441
ATCAACG103250.016.892773
GCTATTG12850.016.8224748
AACGCAG106300.016.7548716
TCAACGC105550.016.6134594
TATCAAC110500.016.465152
AGAGTAC69300.016.4432310-11
CGATAAT4500.016.19078866-67
Produced by FastQC (version 0.11.5)