##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146525_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2868580
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.098068730870327	31.0	31.0	34.0	28.0	34.0
2	31.134314190296244	31.0	31.0	34.0	27.0	34.0
3	31.345901805074288	33.0	31.0	34.0	28.0	34.0
4	34.82484469667919	37.0	35.0	37.0	32.0	37.0
5	34.850242977361624	37.0	35.0	37.0	32.0	37.0
6	34.85417802536447	37.0	35.0	37.0	32.0	37.0
7	34.85429933974301	37.0	35.0	37.0	32.0	37.0
8	34.80107544499369	37.0	35.0	37.0	32.0	37.0
9	36.39386177132937	39.0	37.0	39.0	32.0	39.0
10-11	36.38679154843163	39.0	37.0	39.0	32.0	39.0
12-13	36.332736057561576	39.0	37.0	39.0	32.0	39.0
14-15	37.65358121439876	40.0	38.0	41.0	32.0	41.0
16-17	37.676391629307886	40.0	38.0	41.0	31.5	41.0
18-19	37.690614171471594	40.0	38.0	41.0	31.0	41.0
20-21	37.79832530380885	40.0	38.0	41.0	32.0	41.0
22-23	37.80947698861458	40.0	38.0	41.0	31.0	41.0
24-25	37.826894317048854	40.0	38.0	41.0	31.0	41.0
26-27	37.89023175229556	40.0	38.0	41.0	31.5	41.0
28-29	37.87305583250249	40.0	38.0	41.0	31.0	41.0
30-31	37.83621129618139	40.0	38.0	41.0	31.0	41.0
32-33	37.806442909035134	40.0	38.0	41.0	31.0	41.0
34-35	37.75548389795648	40.0	38.0	41.0	31.0	41.0
36-37	37.674832495520434	40.0	38.0	41.0	30.5	41.0
38-39	37.616679158329205	40.0	38.0	41.0	30.0	41.0
40-41	37.567428658081695	40.0	37.0	41.0	30.0	41.0
42-43	37.411126933883665	40.0	37.0	41.0	30.0	41.0
44-45	37.28694580593883	40.0	37.0	41.0	30.0	41.0
46-47	37.17480809320291	40.0	36.5	41.0	30.0	41.0
48-49	37.07308145493589	40.0	36.0	41.0	30.0	41.0
50-51	36.953033208068106	40.0	36.0	41.0	30.0	41.0
52-53	36.839257228315056	40.0	35.5	41.0	30.0	41.0
54-55	36.72091348332624	39.0	35.0	41.0	30.0	41.0
56-57	36.54609545489406	39.0	35.0	41.0	29.0	41.0
58-59	36.37004319210202	39.0	35.0	41.0	29.0	41.0
60-61	36.147006184244475	38.5	35.0	40.5	29.0	41.0
62-63	35.92695357981998	38.0	35.0	40.0	29.0	41.0
64-65	35.64367892825021	37.5	34.5	40.0	28.0	41.0
66-67	35.39799552391776	37.0	34.0	40.0	28.0	41.0
68-69	35.143024248931525	36.5	34.0	39.5	28.0	41.0
70-71	34.83835782861207	36.0	34.0	39.0	28.0	41.0
72-73	34.493354551729425	35.5	34.0	39.0	27.5	40.5
74-75	34.16630597717337	35.0	34.0	37.5	27.0	40.0
76-77	33.8938093063467	35.0	33.5	37.0	27.0	39.0
78-79	33.57593460876113	35.0	33.0	37.0	27.0	39.0
80-81	33.329486366076594	35.0	33.0	36.0	26.5	39.0
82-83	33.07422400630277	35.0	33.0	36.0	26.0	37.0
84-85	32.88086561992344	35.0	33.0	35.5	26.0	37.0
86-87	32.692383165189746	35.0	33.0	35.0	26.0	37.0
88-89	32.523402171109055	35.0	33.0	35.0	26.0	36.0
90-91	32.39787525535282	35.0	33.0	35.0	26.0	36.0
92-93	32.27083679032832	35.0	33.0	35.0	25.0	36.0
94-95	32.16850253435498	35.0	33.0	35.0	25.0	35.5
96-97	32.104976155449734	35.0	33.0	35.0	25.0	35.0
98-99	32.03060259780101	35.0	32.5	35.0	25.0	35.0
100	30.89987310794888	34.0	30.0	35.0	22.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3006174836847606
1101	2	0.18777248990063455
1101	3	0.2890656229890496
1101	4	0.26250290618649785
1101	5	0.288418535280897
1101	6	0.27108723903197074
1101	7	0.28783683234942004
1101	8	0.22677127366576855
1101	9	0.4534730856417468
1101	10-11	0.4176129870913954
1101	12-13	0.3111022594197337
1101	14-15	0.4584130553845256
1101	16-17	0.4575076160746647
1101	18-19	0.4156737675525335
1101	20-21	0.41000297004345043
1101	22-23	0.5187903739753992
1101	24-25	0.41821839554993545
1101	26-27	0.4327672843974568
1101	28-29	0.43541134808352666
1101	30-31	0.4659947846256074
1101	32-33	0.5078270644186347
1101	34-35	0.4606764945873394
1101	36-37	0.47512578637535796
1101	38-39	0.4556407145405146
1101	40-41	0.5153042904968999
1101	42-43	0.5340676993435309
1101	44-45	0.47223254745619414
1101	46-47	0.42844571649899876
1101	48-49	0.49320303215176864
1101	50-51	0.4760375436568083
1101	52-53	0.4207782392670296
1101	54-55	0.4413909210772573
1101	56-57	0.4490577653064136
1101	58-59	0.44799010789641613
1101	60-61	0.48761940594206266
1101	62-63	0.4340403388785816
1101	64-65	0.4479916000483044
1101	66-67	0.3631583859728664
1101	68-69	0.34036147488181
1101	70-71	0.4367427510975901
1101	72-73	0.3520328728878894
1101	74-75	0.3752073332104686
1101	76-77	0.34867755881561635
1101	78-79	0.3424095408591583
1101	80-81	0.3425225623074226
1101	82-83	0.3204986761926989
1101	84-85	0.3771952807613985
1101	86-87	0.3262032229983376
1101	88-89	0.2890292904115199
1101	90-91	0.2652164928171459
1101	92-93	0.25600444777347775
1101	94-95	0.1939791212116333
1101	96-97	0.2847697035124881
1101	98-99	0.3530580608692446
1101	100	0.32882859782765195
1102	1	0.34891825295732914
1102	2	0.39197889825051746
1102	3	0.4270703160285585
1102	4	0.41231921006302485
1102	5	0.45983937184402635
1102	6	0.414857595035393
1102	7	0.5182008090572126
1102	8	0.4409314003512179
1102	9	0.5303107954118502
1102	10-11	0.5085374393575748
1102	12-13	0.4861012336490447
1102	14-15	0.6261358500052765
1102	16-17	0.5201176784969732
1102	18-19	0.5313025899345121
1102	20-21	0.4952727920916402
1102	22-23	0.5272425165054031
1102	24-25	0.5333432734277821
1102	26-27	0.5096234996252207
1102	28-29	0.5584817209428579
1102	30-31	0.5898312972935997
1102	32-33	0.5336187514621002
1102	34-35	0.4709263695542063
1102	36-37	0.45147664319883773
1102	38-39	0.47753939390233313
1102	40-41	0.5122901880906099
1102	42-43	0.6103406114565999
1102	44-45	0.43734352198270443
1102	46-47	0.4490339045000269
1102	48-49	0.4032223582259675
1102	50-51	0.49778339704811714
1102	52-53	0.32310386933973945
1102	54-55	0.3843779625438941
1102	56-57	0.4471022859720861
1102	58-59	0.4553129323476881
1102	60-61	0.42769956576267987
1102	62-63	0.4096002433321999
1102	64-65	0.4783425605577918
1102	66-67	0.40710198632817196
1102	68-69	0.42036999432320954
1102	70-71	0.34684413882671805
1102	72-73	0.4219788591303697
1102	74-75	0.37793907144266115
1102	76-77	0.350545501097443
1102	78-79	0.35105249059861166
1102	80-81	0.3005780365406139
1102	82-83	0.26554432853295395
1102	84-85	0.29673105576164716
1102	86-87	0.2655268769376917
1102	88-89	0.2350204538129148
1102	90-91	0.2588167926748639
1102	92-93	0.2821547387042216
1102	94-95	0.37479754205707394
1102	96-97	0.4148354859093786
1102	98-99	0.3456847102807856
1102	100	0.3179823203771974
1103	1	0.22144481169201313
1103	2	0.261754831907421
1103	3	0.22039584296064163
1103	4	0.23370535539784498
1103	5	0.2028150249230265
1103	6	0.20312875999935898
1103	7	0.26853456099467365
1103	8	0.29513554340069703
1103	9	0.41248787627033323
1103	10-11	0.3865298931697012
1103	12-13	0.40565853150285136
1103	14-15	0.468139662696899
1103	16-17	0.46253344855885814
1103	18-19	0.5257582065836459
1103	20-21	0.45521124597164686
1103	22-23	0.42380416444802194
1103	24-25	0.4598542080463375
1103	26-27	0.44102404154785546
1103	28-29	0.39572037620538225
1103	30-31	0.5028189145310336
1103	32-33	0.4579878754481115
1103	34-35	0.44803112806488343
1103	36-37	0.3783835546923484
1103	38-39	0.37719178277425414
1103	40-41	0.3804157467008409
1103	42-43	0.42361276636275846
1103	44-45	0.4013555879637636
1103	46-47	0.34295311390391703
1103	48-49	0.3021499679964137
1103	50-51	0.3387216076851942
1103	52-53	0.32689605501895613
1103	54-55	0.3491112721186198
1103	56-57	0.3569680498114778
1103	58-59	0.36676189658531655
1103	60-61	0.30916247404312003
1103	62-63	0.32569132932706424
1103	64-65	0.2224898809257141
1103	66-67	0.2731585657667921
1103	68-69	0.29658691611211907
1103	70-71	0.29379525830990616
1103	72-73	0.26895288870418455
1103	74-75	0.27339693384635666
1103	76-77	0.24723584152441447
1103	78-79	0.22883281394201305
1103	80-81	0.24348029247744307
1103	82-83	0.31026638105080195
1103	84-85	0.3357460915692698
1103	86-87	0.2999238324412019
1103	88-89	0.20787128932411747
1103	90-91	0.26246543673691036
1103	92-93	0.2061293000184108
1103	94-95	0.13139055702499292
1103	96-97	0.18082163803237705
1103	98-99	0.24829419788670748
1103	100	0.25013168285443044
1104	1	0.15061256528284872
1104	2	0.11564142309859449
1104	3	0.16695438328616063
1104	4	0.20134468059642074
1104	5	0.15894751107475713
1104	6	0.15423335053807818
1104	7	0.11662072177360017
1104	8	0.19685237457629512
1104	9	0.21524224748866772
1104	10-11	0.26750976738671994
1104	12-13	0.23137616568927655
1104	14-15	0.357036883860566
1104	16-17	0.34109394790916525
1104	18-19	0.31154362463591667
1104	20-21	0.23151122835391647
1104	22-23	0.29595283246094795
1104	24-25	0.2762069813872614
1104	26-27	0.31961637861735426
1104	28-29	0.3332112941879828
1104	30-31	0.38730379093357215
1104	32-33	0.3285977960165738
1104	34-35	0.3628606254069453
1104	36-37	0.339324638766044
1104	38-39	0.27561522560664997
1104	40-41	0.3459050856363106
1104	42-43	0.2898900661532693
1104	44-45	0.3156312646141828
1104	46-47	0.3290144826314574
1104	48-49	0.3044037348492452
1104	50-51	0.3416526555501207
1104	52-53	0.34580470987921075
1104	54-55	0.3635522390541439
1104	56-57	0.34584203138540914
1104	58-59	0.26776701532541836
1104	60-61	0.32030655113475603
1104	62-63	0.30356190984151254
1104	64-65	0.40941224273461074
1104	66-67	0.32275635032840455
1104	68-69	0.28896440849165117
1104	70-71	0.25654428390387096
1104	72-73	0.25468615384902904
1104	74-75	0.29137587543218046
1104	76-77	0.3165468804138385
1104	78-79	0.32361597346150717
1104	80-81	0.23283472533516658
1104	82-83	0.22459811413347097
1104	84-85	0.2664629674214254
1104	86-87	0.2817783750450289
1104	88-89	0.31382744573719634
1104	90-91	0.24124011769670517
1104	92-93	0.2452710199516588
1104	94-95	0.2604791183420119
1104	96-97	0.27431559687379803
1104	98-99	0.15706545061796362
1104	100	0.30296861491993354
1105	1	0.21603395396438074
1105	2	0.12788267061802827
1105	3	0.2168217191205244
1105	4	0.2802818561545024
1105	5	0.10976306070716646
1105	6	0.22834179911659191
1105	7	0.30325752406299955
1105	8	0.284641775821477
1105	9	0.26850521178697306
1105	10-11	0.34371717285618075
1105	12-13	0.3641759474542212
1105	14-15	0.45619948129261445
1105	16-17	0.4162075654561832
1105	18-19	0.3989185798684787
1105	20-21	0.39582953778655394
1105	22-23	0.35602366035657695
1105	24-25	0.4583942473210669
1105	26-27	0.41304715255962776
1105	28-29	0.44090521519201076
1105	30-31	0.4087692775597418
1105	32-33	0.4301410931473626
1105	34-35	0.3859389109283953
1105	36-37	0.3549711243953837
1105	38-39	0.3838131124815263
1105	40-41	0.40136433452116194
1105	42-43	0.3414646456847166
1105	44-45	0.2957829012239799
1105	46-47	0.2897874573279964
1105	48-49	0.3891058271154222
1105	50-51	0.36937288055758444
1105	52-53	0.3523201999978838
1105	54-55	0.2177574231338113
1105	56-57	0.2699910236608787
1105	58-59	0.25454699533375447
1105	60-61	0.32793763106015916
1105	62-63	0.27496834819620375
1105	64-65	0.31704639774672927
1105	66-67	0.26599169109876186
1105	68-69	0.3038994199574603
1105	70-71	0.3160226170651299
1105	72-73	0.29969419201758996
1105	74-75	0.3482508954397616
1105	76-77	0.2779568244874966
1105	78-79	0.29932128694107263
1105	80-81	0.18609364757612923
1105	82-83	0.22619403608801747
1105	84-85	0.2418536550858832
1105	86-87	0.2236471640981037
1105	88-89	0.23618261741141566
1105	90-91	0.20492892925196315
1105	92-93	0.12370854797353559
1105	94-95	0.1513612279860297
1105	96-97	0.23718323415074138
1105	98-99	0.24133306676569788
1105	100	0.29213271806338525
1106	1	0.1826480209696264
1106	2	0.14548715391325473
1106	3	0.13696020762285954
1106	4	0.14889583065788514
1106	5	0.17584426527675845
1106	6	0.19537936377812315
1106	7	0.2017550008017963
1106	8	0.2576308873181077
1106	9	0.3916949977428317
1106	10-11	0.31429968466093783
1106	12-13	0.2946859630789831
1106	14-15	0.3299483720602723
1106	16-17	0.3117691997055445
1106	18-19	0.32493036207772263
1106	20-21	0.35307526323816063
1106	22-23	0.30236025624783025
1106	24-25	0.3684419317437744
1106	26-27	0.3371026352915081
1106	28-29	0.32886371345588117
1106	30-31	0.38580862044030084
1106	32-33	0.43537787636925174
1106	34-35	0.3693088284609658
1106	36-37	0.40754475889409747
1106	38-39	0.39228453069882363
1106	40-41	0.3135631468776907
1106	42-43	0.4029165916854609
1106	44-45	0.3372679180768401
1106	46-47	0.2999251730663275
1106	48-49	0.35894533638442283
1106	50-51	0.3149051986111573
1106	52-53	0.2872983715692996
1106	54-55	0.20182383372544876
1106	56-57	0.25278050637150073
1106	58-59	0.2281756336804861
1106	60-61	0.22858034663905613
1106	62-63	0.2991843015499853
1106	64-65	0.3490565809952173
1106	66-67	0.29760032433778605
1106	68-69	0.22268749618014994
1106	70-71	0.21894728464015145
1106	72-73	0.3210262992703363
1106	74-75	0.19903617017747877
1106	76-77	0.24475965849175907
1106	78-79	0.27605347767531185
1106	80-81	0.20681805781211438
1106	82-83	0.2097644700797474
1106	84-85	0.23799431652016523
1106	86-87	0.16836968867502122
1106	88-89	0.12389193519294395
1106	90-91	0.21796094782358466
1106	92-93	0.2608410074802734
1106	94-95	0.27431757601250695
1106	96-97	0.2768916407027504
1106	98-99	0.2770139077627505
1106	100	0.28562236805300145
1107	1	0.07540740072793994
1107	2	0.06930338535761393
1107	3	0.03303978515592121
1107	4	0.05656074274848777
1107	5	0.08826592241016584
1107	6	0.18012253604403838
1107	7	0.0602114681159307
1107	8	0.10483820293079305
1107	9	0.06426772330470953
1107	10-11	0.08812846236964589
1107	12-13	0.1429838056918058
1107	14-15	0.09332853527575935
1107	16-17	0.1637050312662396
1107	18-19	0.23042576843412377
1107	20-21	0.25484514193859553
1107	22-23	0.2799514875496172
1107	24-25	0.23139794568153604
1107	26-27	0.21129083495959833
1107	28-29	0.29063510341088516
1107	30-31	0.22974692133393404
1107	32-33	0.20272023540911732
1107	34-35	0.2066942725131824
1107	36-37	0.16876839035328572
1107	38-39	0.18325749349914844
1107	40-41	0.16259480338377585
1107	42-43	0.16796255663576432
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2216	56-57	-0.46585250895187613
2216	58-59	-0.4717107210042073
2216	60-61	-0.5002404820705451
2216	62-63	-0.48384979504146486
2216	64-65	-0.466049640663023
2216	66-67	-0.4045407414150901
2216	68-69	-0.5076581790900789
2216	70-71	-0.472111896548455
2216	72-73	-0.4258053960634598
2216	74-75	-0.41122382478779684
2216	76-77	-0.44925939556136996
2216	78-79	-0.3853867674509317
2216	80-81	-0.4444746603252341
2216	82-83	-0.45111129454836885
2216	84-85	-0.45970768915160676
2216	86-87	-0.42999087757791443
2216	88-89	-0.350792666412687
2216	90-91	-0.45945465195995894
2216	92-93	-0.38573955075818844
2216	94-95	-0.46675601152328383
2216	96-97	-0.40451376803508055
2216	98-99	-0.37220969073299415
2216	100	-0.4587777370940671
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	904.0
1	213.0
2	150.0
3	140.0
4	133.0
5	140.0
6	167.0
7	375.0
8	1021.0
9	1615.0
10	2180.0
11	3397.0
12	4993.0
13	6445.0
14	7929.0
15	8674.0
16	9408.0
17	10265.0
18	11598.0
19	12782.0
20	14145.0
21	15665.0
22	17146.0
23	19533.0
24	23099.0
25	27852.0
26	30539.0
27	28611.0
28	30219.0
29	37202.0
30	43022.0
31	49251.0
32	61657.0
33	81599.0
34	118083.0
35	180549.0
36	327530.0
37	656401.0
38	796581.0
39	227054.0
40	313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.930862962902225	19.515195967421985	13.498640372746879	24.055300696928914
2	14.832489986932956	21.066195425209138	39.99896747331651	24.102347114541395
3	18.20334392060107	26.225746039543168	30.331370781373852	25.23953925848191
4	12.369192831589238	17.680433124628	39.14153096633152	30.808843077451243
5	13.129987356292967	38.64330651081135	35.03294202036948	13.193764112526202
6	30.013890522030312	38.34513324049724	18.18140582131855	13.459570416153898
7	26.239029926802793	33.36409530325778	22.91324810464978	17.48362666528965
8	25.073612818382635	36.00292253094809	20.663089334332167	18.260375316337107
9	25.80533298818772	16.585688853319976	20.484415593930137	37.124562564562176
10-11	24.322066909831573	26.79514086532817	28.6837014678338	20.19909075700646
12-13	24.971768874909863	24.715203154211192	28.121043761470805	22.19198420940814
14-15	22.366147100859077	26.03584482488836	25.7238890314473	25.874119042805262
16-17	22.23065374794111	28.850767925917943	26.392972364017226	22.525605962123727
18-19	22.258791669632227	28.60369836956901	27.342427490269465	21.79508247052929
20-21	23.34057388194051	27.61626522699931	26.811379958559158	22.231780932501028
22-23	23.108262673318293	27.464090862072243	26.699307293746283	22.728339170863183
24-25	22.958477095181202	27.66397485526128	26.803872119507982	22.573675930049536
26-27	22.738352957511268	27.694589497013684	26.80520894983219	22.76184859564286
28-29	22.570872943658905	28.06441573955711	26.701505378444914	22.663205938339072
30-31	22.903558556020386	27.705228811417133	26.565528617934486	22.825684014627992
32-33	22.798384564390638	27.874114650570466	26.42688671016364	22.900614074875268
34-35	23.128472225250977	27.79055476226406	26.67040584637903	22.410567166105935
36-37	23.053878687338855	27.442790846814617	26.752343520616684	22.75098694522984
38-39	22.89061661777444	27.74829705887741	26.717434331327922	22.64365199202023
40-41	22.91094212213994	27.570639229441923	26.815265390044175	22.703153258373966
42-43	22.787071948817044	27.575704285553783	26.50574642858887	23.1314773370403
44-45	22.84044145008408	27.60409824725943	26.706836872781032	22.848623429875463
46-47	23.022937544321177	27.59324564900158	26.581540933359264	22.802275873317974
48-49	22.884870541621996	27.610500430495666	26.577206148542377	22.92742287933996
50-51	22.64080270872674	27.716978669893464	26.71252855900706	22.92969006237273
52-53	22.879382346947605	27.750612739966556	26.417083301928667	22.95292161115717
54-55	22.558780233610147	27.52424323731175	26.7127101798743	23.2042663492038
56-57	22.823827032874046	27.494794324570698	26.641787594485084	23.03959104807017
58-59	22.974541126295673	27.462300742967045	26.632323000747277	22.930835129990008
60-61	22.633775011798736	27.583400287357073	26.864606314702232	22.918218386141955
62-63	22.666325159374917	27.48412531968328	26.801872613825395	23.04767690711641
64-65	22.44572825191241	27.661985602442968	26.604981794116572	23.28730435152805
66-67	22.600508617267685	27.50651038826778	26.55865020130949	23.334330793155043
68-69	22.538019360640345	27.426910808521864	26.71183521297158	23.323234617866213
70-71	22.695173065508023	27.241420704350045	26.650123584570196	23.413282645571737
72-73	22.676324957266477	27.229309726039613	26.906180269596746	23.188185047097164
74-75	22.562122942147006	27.58282012054684	26.79192338938194	23.06313354792421
76-77	22.76000387339579	27.191884014478124	26.725890074074332	23.322222038051752
78-79	22.74643400987992	27.22433034136873	26.737645342877187	23.29159030587416
80-81	22.744672442567417	27.33402718587542	26.76991285549401	23.151387516063153
82-83	22.901208771554828	27.038793235067025	27.093111616159415	22.966886377218735
84-85	22.784706762359765	27.290085093293094	26.841281614673544	23.0839265296736
86-87	22.567355696772783	27.46705102628695	26.812956887957263	23.15263638898301
88-89	22.743384695267295	27.037596907745677	26.72963118439524	23.489387212591787
90-91	22.75917154993637	27.06230543835669	26.773828163575374	23.404694848131562
92-93	23.025195540394932	27.06837005007844	26.807701863007278	23.098732546519354
94-95	22.63771992601295	26.89323135747252	27.068725537848927	23.4003231786656
96-97	22.583047666933187	26.81147448175733	27.223256295143038	23.382221556166442
98-99	22.56440053218173	27.069502061113653	26.857292416410388	23.508804990294227
100	22.749795304890004	26.687384875381476	26.71950007915227	23.84331974057625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1992.0
1	1422.5
2	889.0
3	907.0
4	845.0
5	794.0
6	745.0
7	664.0
8	641.5
9	671.0
10	607.5
11	537.0
12	567.0
13	628.5
14	670.0
15	745.5
16	844.0
17	960.5
18	1193.5
19	1467.5
20	1889.0
21	2646.5
22	3848.0
23	5434.5
24	7370.0
25	9779.5
26	13078.0
27	17481.0
28	22243.5
29	27450.0
30	33639.0
31	40824.5
32	48278.0
33	53330.5
34	57439.0
35	62139.5
36	67714.5
37	74602.5
38	78500.0
39	79498.5
40	81850.0
41	84463.5
42	87376.5
43	92146.0
44	97575.5
45	103808.0
46	108968.0
47	114713.0
48	122077.5
49	120415.5
50	111714.0
51	107131.0
52	104746.0
53	102380.5
54	99934.5
55	93545.5
56	85554.0
57	76542.0
58	67104.0
59	59560.0
60	49728.0
61	40267.0
62	32622.0
63	24883.5
64	18587.0
65	14078.5
66	10805.0
67	8094.0
68	5889.0
69	4364.5
70	3237.5
71	2485.0
72	1901.0
73	1486.5
74	1162.5
75	866.5
76	637.0
77	470.5
78	359.5
79	255.5
80	187.5
81	134.0
82	109.5
83	87.5
84	63.5
85	45.0
86	39.5
87	25.5
88	21.0
89	20.5
90	10.5
91	8.5
92	9.0
93	8.5
94	5.5
95	7.0
96	9.0
97	6.0
98	5.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09485529425708887
2	0.06365518828130992
3	0.07822685788787484
4	0.08150374052667174
5	0.0812945778050464
6	0.0905325980101653
7	0.08669794811370085
8	0.08962622621645552
9	0.07519399842430749
10-11	0.08190463574312029
12-13	0.0719171157855106
14-15	0.08047535714534718
16-17	0.08202664733073507
18-19	0.08171290324829707
20-21	0.07266661553800138
22-23	0.07716361405294606
24-25	0.07998731079488806
26-27	0.07287577825962671
28-29	0.08101569417621263
30-31	0.08476319293866652
32-33	0.0864539249384713
34-35	0.08828409875269297
36-37	0.08852812192792252
38-39	0.09002712143290409
40-41	0.08539068110354252
42-43	0.09154355116468775
44-45	0.0880400755774634
46-47	0.08462375112424962
48-49	0.09403607359738965
50-51	0.09007941211331041
52-53	0.09635429376207043
54-55	0.08795292444345286
56-57	0.09473328266947409
58-59	0.09902111846279345
60-61	0.09787072349385409
62-63	0.0994568741328462
64-65	0.10058983887498345
66-67	0.10344839607052966
68-69	0.10001464139051378
70-71	0.10217598951397557
72-73	0.09954402526685677
74-75	0.10360526811174867
76-77	0.10031095524614965
78-79	0.10707388324536879
80-81	0.10240258246240301
82-83	0.1069344414309519
84-85	0.10320437289530011
86-87	0.09973575776168
88-89	0.0989688277823871
90-91	0.09910826959680401
92-93	0.09328657384489887
94-95	0.0931819924840862
96-97	0.09135181866986454
98-99	0.10519141875074077
100	0.24426719840478567
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2868580.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.51373734168662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08527052525199	43.62149126769523
2	15.342035235245055	18.56807781025041
3	5.742308752467495	10.424656907450586
4	2.6796703338796255	6.486274669868054
5	1.426026296317379	4.314709036884403
6	0.8201923228880448	2.977974167814897
7	0.5171498688512791	2.190626993096779
8	0.3425113058784924	1.658131136039134
9	0.23149853335998755	1.2607957298458767
>10	0.795902662706848	7.335734056454759
>50	0.014178599040271647	0.5760704966288231
>100	0.002998705565265213	0.2782151232069245
>500	8.561951612350114E-5	0.03243404225672426
>1k	1.7123903224700222E-4	0.2748085625074528
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4174	0.14550753334402386	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.394418144168892E-4
2	0.0	0.0	0.0	0.0	1.743022680211115E-4
3	0.0	0.0	0.0	1.0458136081266689E-4	1.743022680211115E-4
4	0.0	0.0	0.0	1.394418144168892E-4	1.743022680211115E-4
5	0.0	0.0	0.0	1.394418144168892E-4	1.743022680211115E-4
6	0.0	0.0	0.0	1.394418144168892E-4	3.834649896464453E-4
7	0.0	0.0	0.0	1.394418144168892E-4	3.834649896464453E-4
8	0.0	0.0	0.0	1.743022680211115E-4	3.834649896464453E-4
9	0.0	0.0	0.0	1.743022680211115E-4	3.834649896464453E-4
10-11	0.0	0.0	0.0	2.0916272162533378E-4	3.834649896464453E-4
12-13	0.0	0.0	0.0	2.2659294842744494E-4	0.0010981042885330025
14-15	0.0	0.0	0.0	2.614534020316672E-4	0.0013072670101583362
16-17	0.0	0.0	0.0	3.137440824380007E-4	0.0014815692781794476
18-19	0.0	0.0	0.0	3.4860453604222295E-4	0.0016907319998047815
20-21	0.0	0.0	0.0	4.531858968548899E-4	0.001987045855440671
22-23	0.0	0.0	0.0	5.92627711271779E-4	0.0020219063090448934
24-25	0.0	0.0	0.0	9.760927009182243E-4	0.0020567667626491155
26-27	0.0	0.0	0.0	0.001411848370971003	0.0022310690306702272
28-29	0.0	0.0	0.0	0.00364291740164123	0.0022659294842744494
30-31	0.0	0.0	0.0	0.010214112906037133	0.0022833597110765605
32-33	0.0	0.0	0.0	0.027557188574137727	0.0023007899378786716
34-35	0.0	0.0	0.0	0.05302274993202211	0.0024053712986913385
36-37	0.0	0.0	0.0	0.09548278242196487	0.0024053712986913385
38-39	0.0	0.0	0.0	0.16103786542470488	0.002457661979097672
40-41	0.0	0.0	0.0	0.2379748865292235	0.0024750922058997833
42-43	0.0	0.0	0.0	0.3129945826855099	0.0024925224327018943
44-45	0.0	0.0	0.0	0.40832049306625573	0.0026493944739208945
46-47	0.0	0.0	0.0	0.5320053824540365	0.002806266515139895
48-49	0.0	0.0	0.0	0.6666887449539494	0.0029631385563588953
50-51	0.0	0.0	0.0	0.810906441514617	0.003137440824380007
52-53	0.0	0.0	0.0	0.9509931743231843	0.003242022185192674
54-55	0.0	0.0	0.0	1.1069414135216729	0.003416324453213785
56-57	0.0	0.0	0.0	1.2973666413347371	0.003468615133620119
58-59	0.0	0.0	0.0	1.5121070355367463	0.0035557662676306743
60-61	0.0	0.0	0.0	1.750604828870033	0.0035557662676306743
62-63	0.0	0.0	0.0	1.9874293204303175	0.003869510350068675
64-65	0.0	0.0	0.0	2.2304241122785493	0.003886940576870786
66-67	0.0	0.0	0.0	2.5067977884528236	0.0039043708036728972
68-69	0.0	0.0	0.0	2.8267296014055736	0.003921801030475009
70-71	0.0	0.0	0.0	3.179970577777158	0.003974091710881342
72-73	0.0	0.0	0.0	3.534815135014537	0.004165824205704565
74-75	0.0	0.0	0.0	3.88969455270552	0.004357556700527787
76-77	0.0	0.0	0.0	4.274588820949738	0.004409847380934121
78-79	0.0	0.0	0.0	4.7176303258058	0.0044621380613404545
80-81	0.0	0.0	0.0	5.198948608719297	0.004479568288142565
82-83	0.0	0.0	0.0	5.69525688668261	0.004741021690174233
84-85	0.0	0.0	0.0	6.187381910213416	0.004741021690174233
86-87	0.0	0.0	0.0	6.679942689414275	0.005054765772612234
88	0.0	0.0	0.0	7.072000780874161	0.005368509855050234
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	9665	0.0	26.3229	1
TACATGG	9610	0.0	25.34098	2
ACATGGG	9435	0.0	24.61513	3
CATGGGG	6775	0.0	21.441671	4
GTACTAG	985	0.0	19.574287	1
GTATCAA	9765	0.0	18.637047	1
GGTATCA	7880	0.0	18.380733	1
ATGGGGG	4305	0.0	18.345217	5
TAGTACT	1465	0.0	17.649586	4
CTAGTAC	1120	0.0	17.629837	3
CAACGCA	10170	0.0	17.241482	5
GAGTACA	7295	0.0	16.953869	1
GTATTAG	860	0.0	16.951244	1
ATCAACG	10325	0.0	16.89277	3
GCTATTG	1285	0.0	16.822474	8
AACGCAG	10630	0.0	16.754871	6
TCAACGC	10555	0.0	16.613459	4
TATCAAC	11050	0.0	16.46515	2
AGAGTAC	6930	0.0	16.44323	10-11
CGATAAT	450	0.0	16.190788	66-67
>>END_MODULE