FastQCFastQC Report
Mon 16 Jan 2017
ERR1146525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868580
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119980.4182557223434591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106620.3716821563282181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59400.20707109440908045No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45360.15812701754875236No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110050.033.7589151
GTATCAA166450.031.7848531
ATCAACG198450.026.3268723
TATCAAC200700.026.3128992
CAACGCA198450.026.2880445
TCAACGC200450.026.0876544
AACGCAG204050.025.703896
GAGTACT132350.023.32989112-13
ACGCAGA223900.023.2571747
CGCAGAG227000.022.856758
GTACATG89000.022.509771
TACATGG88100.022.205152
AGAGTAC175250.022.16440610-11
GTACTTT140800.021.61266914-15
GCAGAGT239000.021.2961869
ACATGGG86400.021.2813573
AGTACTT139000.020.77678512-13
GTACTAG8750.018.8109821
ACTTTTT161750.018.71168316-17
TACTTTT153650.018.50521314-15
Produced by FastQC (version 0.11.5)