##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146525_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2868580
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33600283066883	33.0	31.0	34.0	30.0	34.0
2	32.42509150869071	34.0	31.0	34.0	30.0	34.0
3	32.51652524942654	34.0	31.0	34.0	30.0	34.0
4	36.01262297025009	37.0	35.0	37.0	35.0	37.0
5	36.050159660877505	37.0	35.0	37.0	35.0	37.0
6	36.11733401195016	37.0	36.0	37.0	35.0	37.0
7	36.0823009991006	37.0	36.0	37.0	35.0	37.0
8	36.09213304143513	37.0	36.0	37.0	35.0	37.0
9	37.872771894107885	39.0	38.0	39.0	35.0	39.0
10-11	37.821099463846224	39.0	38.0	39.0	35.0	39.0
12-13	37.78927849319175	39.0	38.0	39.0	35.0	39.0
14-15	39.12650335706168	40.0	39.0	41.0	36.0	41.0
16-17	39.07952732710958	40.0	38.5	41.0	36.0	41.0
18-19	39.189557202518316	40.0	39.0	41.0	36.0	41.0
20-21	39.24983842179754	40.0	39.0	41.0	36.0	41.0
22-23	39.214167462647026	40.0	39.0	41.0	36.0	41.0
24-25	39.2027067399201	41.0	39.0	41.0	36.0	41.0
26-27	39.11945474764518	40.0	39.0	41.0	35.5	41.0
28-29	39.05782024555703	40.0	39.0	41.0	35.0	41.0
30-31	38.90688249935508	40.0	38.5	41.0	35.0	41.0
32-33	38.78287933402589	40.0	38.0	41.0	35.0	41.0
34-35	38.701261425513664	40.0	38.0	41.0	35.0	41.0
36-37	38.62060618842773	40.0	38.0	41.0	35.0	41.0
38-39	38.53254937983253	40.0	38.0	41.0	35.0	41.0
40-41	38.42913601851787	40.0	38.0	41.0	34.0	41.0
42-43	38.319228154696745	40.0	38.0	41.0	34.0	41.0
44-45	38.173868429676006	40.0	38.0	41.0	33.5	41.0
46-47	37.9218768519616	40.0	38.0	41.0	33.0	41.0
48-49	37.63249656624532	40.0	37.0	41.0	33.0	41.0
50-51	37.525525695640354	40.0	37.0	41.0	33.0	41.0
52-53	37.43115234715434	40.0	37.0	41.0	32.0	41.0
54-55	37.271547072070504	40.0	36.0	41.0	32.0	41.0
56-57	37.12955399535659	40.0	36.0	41.0	32.0	41.0
58-59	36.959139365121416	39.0	35.5	41.0	31.5	41.0
60-61	36.753811293392545	39.0	35.0	41.0	31.0	41.0
62-63	36.527723647240094	39.0	35.0	41.0	31.0	41.0
64-65	36.262335022903315	38.0	35.0	40.5	31.0	41.0
66-67	35.99138040424182	37.0	35.0	40.0	31.0	41.0
68-69	35.69416087402129	37.0	35.0	40.0	30.5	41.0
70-71	35.37603361244936	36.0	35.0	39.0	30.0	41.0
72-73	35.04751009210132	36.0	35.0	39.0	30.0	41.0
74-75	34.728166723605405	35.5	34.5	38.5	30.0	40.0
76-77	34.4273077620286	35.0	34.0	37.0	29.5	39.5
78-79	34.1382595221329	35.0	34.0	37.0	29.0	39.0
80-81	33.871220255317965	35.0	34.0	36.5	29.0	39.0
82-83	33.620877925663564	35.0	34.0	36.0	29.0	37.5
84-85	33.41163223615865	35.0	34.0	36.0	29.0	37.0
86-87	33.23006853565178	35.0	34.0	35.0	29.0	37.0
88-89	33.03147463204791	35.0	34.0	35.0	29.0	36.0
90-91	32.94760386672151	35.0	34.0	35.0	29.0	36.0
92-93	32.848134791429906	35.0	34.0	35.0	29.0	36.0
94-95	32.77597940444401	35.0	34.0	35.0	29.0	36.0
96-97	32.72000711153254	35.0	34.0	35.0	29.0	35.0
98-99	32.65160427807487	35.0	34.0	35.0	28.0	35.0
100	31.9351619965279	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2956307584576763
1101	2	0.28200354121741356
1101	3	0.3058149963326997
1101	4	0.18972446454532133
1101	5	0.1505221668059633
1101	6	0.19700865939961432
1101	7	0.22185524548179814
1101	8	0.23702172489089435
1101	9	0.25087929155405675
1101	10-11	0.2208106892952273
1101	12-13	0.28560504095905515
1101	14-15	0.3951755294853925
1101	16-17	0.43343181286294197
1101	18-19	0.35953902798004833
1101	20-21	0.3536653199750006
1101	22-23	0.3308144309226222
1101	24-25	0.3129424919874424
1101	26-27	0.3629075543134803
1101	28-29	0.3950990282262836
1101	30-31	0.42186028687109456
1101	32-33	0.3041857467679421
1101	34-35	0.31639911888526484
1101	36-37	0.2830519752457832
1101	38-39	0.3139765838352204
1101	40-41	0.2970992484981636
1101	42-43	0.29794813366901707
1101	44-45	0.23859126608930126
1101	46-47	0.19855966650099077
1101	48-49	0.3043883228152282
1101	50-51	0.32832538108257836
1101	52-53	0.2939259900151612
1101	54-55	0.2585083477306327
1101	56-57	0.2109084869712632
1101	58-59	0.18324722028959428
1101	60-61	0.13672053917267846
1101	62-63	0.1611842551737368
1101	64-65	0.16135938905833314
1101	66-67	0.13458026374301824
1101	68-69	0.06483775611170728
1101	70-71	0.13346872188300551
1101	72-73	0.15574345166390202
1101	74-75	0.11532883382323433
1101	76-77	0.1320515674009215
1101	78-79	0.151666759277731
1101	80-81	0.10962044690368344
1101	82-83	0.15595994709818228
1101	84-85	0.10855713655406163
1101	86-87	0.09420222127646127
1101	88-89	0.17098240690255295
1101	90-91	0.12189769553517493
1101	92-93	0.15851575158535525
1101	94-95	0.18766907499312424
1101	96-97	0.1793000843559014
1101	98-99	0.2336442167897772
1101	100	0.27562665820759236
1102	1	0.2511099541647468
1102	2	0.2812669269704031
1102	3	0.27332329398584676
1102	4	0.18159578632570117
1102	5	0.16755758417264843
1102	6	0.21156282064632848
1102	7	0.1939533124862507
1102	8	0.20026808395942908
1102	9	0.27608857649762797
1102	10-11	0.23409837028830793
1102	12-13	0.23860630737000577
1102	14-15	0.4282430495932701
1102	16-17	0.46398716511259863
1102	18-19	0.3908828374816764
1102	20-21	0.33677267879845374
1102	22-23	0.38129912143373446
1102	24-25	0.32604969278676776
1102	26-27	0.3117864701237636
1102	28-29	0.3400764113688197
1102	30-31	0.3973405554788485
1102	32-33	0.3506768624848533
1102	34-35	0.3130095012371257
1102	36-37	0.3348472957353579
1102	38-39	0.3227906570763608
1102	40-41	0.43346915416052667
1102	42-43	0.4584245514781742
1102	44-45	0.3409870921452125
1102	46-47	0.3459442007622968
1102	48-49	0.49048766066638905
1102	50-51	0.539201794012385
1102	52-53	0.4742569618580248
1102	54-55	0.44925241683542083
1102	56-57	0.46378618959978724
1102	58-59	0.4181254197238147
1102	60-61	0.39646972715042494
1102	62-63	0.3884952472215133
1102	64-65	0.3832516088371847
1102	66-67	0.3748241448822611
1102	68-69	0.3282062512007613
1102	70-71	0.36070118702620846
1102	72-73	0.33878952761972414
1102	74-75	0.3871316590729421
1102	76-77	0.38105650052622764
1102	78-79	0.35368536509457726
1102	80-81	0.32740071240640134
1102	82-83	0.3150892091753832
1102	84-85	0.34900464703632395
1102	86-87	0.31948599935069666
1102	88-89	0.3453754833385432
1102	90-91	0.28992177564066424
1102	92-93	0.27077630343890036
1102	94-95	0.2670893703929025
1102	96-97	0.3102684063392189
1102	98-99	0.2937831421813115
1102	100	0.3554601728528226
1103	1	0.2664526438659536
1103	2	0.2324684339452574
1103	3	0.23255747470969368
1103	4	0.16947465134284556
1103	5	0.20973053555800192
1103	6	0.21452274778658165
1103	7	0.1531436529132293
1103	8	0.18452517477389563
1103	9	0.180728859790797
1103	10-11	0.23390533156176474
1103	12-13	0.2427824491976054
1103	14-15	0.3443553486350055
1103	16-17	0.4156596017280094
1103	18-19	0.3359141762229214
1103	20-21	0.33222318734590317
1103	22-23	0.35937473038960377
1103	24-25	0.33510554249657076
1103	26-27	0.3519045843687536
1103	28-29	0.3455720047118902
1103	30-31	0.3985419210333916
1103	32-33	0.4411109097079233
1103	34-35	0.3723367365265844
1103	36-37	0.35577750198274316
1103	38-39	0.3748990925151574
1103	40-41	0.4274495985597184
1103	42-43	0.3757205385353828
1103	44-45	0.376567735664203
1103	46-47	0.4256755587366996
1103	48-49	0.5166405190537446
1103	50-51	0.46298897341253564
1103	52-53	0.35657051331805434
1103	54-55	0.4318060999860407
1103	56-57	0.4149198148246427
1103	58-59	0.3908595834772228
1103	60-61	0.37662350759821805
1103	62-63	0.3944554062910868
1103	64-65	0.40637665833429537
1103	66-67	0.3084407431014924
1103	68-69	0.3883530172010552
1103	70-71	0.36317179988527215
1103	72-73	0.34010685783049865
1103	74-75	0.32806393005688506
1103	76-77	0.30741116569949156
1103	78-79	0.3251948289418891
1103	80-81	0.3320364702495766
1103	82-83	0.2881111632646878
1103	84-85	0.2764482486157078
1103	86-87	0.31339219808582897
1103	88-89	0.3603250245710541
1103	90-91	0.33455049075227095
1103	92-93	0.32189762509656816
1103	94-95	0.34511802780502876
1103	96-97	0.2881827961388552
1103	98-99	0.2804507422220439
1103	100	0.28011425876849216
1104	1	0.23217943642194427
1104	2	0.2240167839311411
1104	3	0.22099091562339623
1104	4	0.14861543365201868
1104	5	0.16606805402760472
1104	6	0.14444909501653314
1104	7	0.13146469500651392
1104	8	0.13893764597810332
1104	9	0.19807594260952044
1104	10-11	0.20644461791313518
1104	12-13	0.2736319022405027
1104	14-15	0.3812729023647279
1104	16-17	0.4288359433328637
1104	18-19	0.40396461002319484
1104	20-21	0.3502653828931699
1104	22-23	0.265806137709518
1104	24-25	0.2730381358850025
1104	26-27	0.2873312704337394
1104	28-29	0.3051385876602595
1104	30-31	0.23736155477771348
1104	32-33	0.21546548424674228
1104	34-35	0.24957648927135523
1104	36-37	0.29309866202473245
1104	38-39	0.2607286100232713
1104	40-41	0.2606894936988553
1104	42-43	0.2586203050306821
1104	44-45	0.23401658506135448
1104	46-47	0.2613532440424393
1104	48-49	0.3142294310719933
1104	50-51	0.23107813121667675
1104	52-53	0.20749992507436588
1104	54-55	0.16550060014649404
1104	56-57	0.16645167274230488
1104	58-59	0.1532731977268753
1104	60-61	0.07938221678684698
1104	62-63	0.11632232345770888
1104	64-65	0.13793501612788361
1104	66-67	0.15279099717751876
1104	68-69	0.16832159966195093
1104	70-71	0.11886583979291032
1104	72-73	0.09863179619922136
1104	74-75	0.06771850312376415
1104	76-77	-0.004822319034843758
1104	78-79	0.0034175501928004337
1104	80-81	-0.048917531767941114
1104	82-83	-0.035570931856945265
1104	84-85	0.03670772667553024
1104	86-87	0.06612909097992059
1104	88-89	0.09432577635622152
1104	90-91	0.0701157324962054
1104	92-93	0.10095409905036945
1104	94-95	0.12824590410141212
1104	96-97	0.11640293341194763
1104	98-99	0.1404626901204935
1104	100	0.17511294213939266
1105	1	0.12724582616263547
1105	2	0.13932559260711486
1105	3	0.13924586157303764
1105	4	0.09025689451760144
1105	5	0.07071111664100016
1105	6	0.09657383879184067
1105	7	0.12123336500208381
1105	8	0.09272065428618248
1105	9	0.13751481255840048
1105	10-11	0.16363304451198957
1105	12-13	0.19829894452421115
1105	14-15	0.28206811258083064
1105	16-17	0.3613520543547821
1105	18-19	0.2613071633313524
1105	20-21	0.21176669067590126
1105	22-23	0.28049225934469035
1105	24-25	0.23393954950596196
1105	26-27	0.2185618810961074
1105	28-29	0.1562331893996003
1105	30-31	0.28560621179953927
1105	32-33	0.2604102188724582
1105	34-35	0.218649975855719
1105	36-37	0.1667068024038869
1105	38-39	0.16817480636623827
1105	40-41	0.20424522607189743
1105	42-43	0.24620455234644822
1105	44-45	0.24439034803715032
1105	46-47	0.25436540979417543
1105	48-49	0.3894300459451898
1105	50-51	0.3207897018970982
1105	52-53	0.201955789982442
1105	54-55	0.2777614753678179
1105	56-57	0.27621536133764124
1105	58-59	0.2881404948223505
1105	60-61	0.2558009623953197
1105	62-63	0.23227981927285413
1105	64-65	0.2805725228043343
1105	66-67	0.29255248696573233
1105	68-69	0.2545677818547105
1105	70-71	0.28753437589607955
1105	72-73	0.3152403935770991
1105	74-75	0.300423737984417
1105	76-77	0.19529081052234432
1105	78-79	0.12292295733037406
1105	80-81	0.17667779631047864
1105	82-83	0.15593829127104897
1105	84-85	0.12143699622171766
1105	86-87	0.17668929910563236
1105	88-89	0.2274691630248924
1105	90-91	0.16645744838377396
1105	92-93	0.20156417943685767
1105	94-95	0.19743085056697396
1105	96-97	0.19716456031780183
1105	98-99	0.21038814989850607
1105	100	0.20446554569578979
1106	1	0.12016713551788172
1106	2	0.13137525863804456
1106	3	0.0835107892046949
1106	4	0.050095516113458416
1106	5	0.061020483661096137
1106	6	0.08778593206014307
1106	7	0.08543675304078846
1106	8	0.1245940956513536
1106	9	0.13170701706450672
1106	10-11	0.12379757717217643
1106	12-13	0.16291313275860375
1106	14-15	0.21798621223636871
1106	16-17	0.3248618507903558
1106	18-19	0.18705686650666564
1106	20-21	0.14257440716975367
1106	22-23	0.15334689841363058
1106	24-25	0.16668946037955834
1106	26-27	0.12800117904718888
1106	28-29	0.1648102091217467
1106	30-31	0.15069572171491075
1106	32-33	0.13321171246153796
1106	34-35	0.1732773815145876
1106	36-37	0.1089898345459801
1106	38-39	0.15876716588478246
1106	40-41	0.12086427726133309
1106	42-43	0.09379811065465304
1106	44-45	0.10809359401942231
1106	46-47	0.14522998907875717
1106	48-49	0.23879007382214468
1106	50-51	0.2317351140201822
1106	52-53	0.1433874028098714
1106	54-55	0.23865352655639782
1106	56-57	0.23001743205790603
1106	58-59	0.1860866374803365
1106	60-61	0.26206988829070355
1106	62-63	0.22634053898904938
1106	64-65	0.1608459548618555
1106	66-67	0.21362745627781266
1106	68-69	0.16753117093908543
1106	70-71	0.12086690486540164
1106	72-73	0.05512771366887392
1106	74-75	0.0658376076906606
1106	76-77	0.0641236007663295
1106	78-79	0.04878613328701675
1106	80-81	0.08201406212521078
1106	82-83	0.10618932466336162
1106	84-85	0.07804299373220402
1106	86-87	0.1060347967211257
1106	88-89	0.11457566742835468
1106	90-91	0.060405930591961976
1106	92-93	0.07837028547903913
1106	94-95	0.09807833487541728
1106	96-97	0.07088052961522351
1106	98-99	0.06365376727231364
1106	100	0.17105117268749126
1107	1	0.08865861831524313
1107	2	0.04065749826028764
1107	3	0.06473958174080963
1107	4	0.06903894956978007
1107	5	0.07059556449895865
1107	6	0.07400870809221516
1107	7	0.05260859694244857
1107	8	0.11586389166049571
1107	9	0.1884911243692784
1107	10-11	0.15133377599625675
1107	12-13	0.09320352174982816
1107	14-15	0.16487687724540478
1107	16-17	0.22936553197837384
1107	18-19	0.15214146824831687
1107	20-21	0.13321660481904019
1107	22-23	0.1268017119020044
1107	24-25	0.13643571218307926
1107	26-27	0.12289427917207973
1107	28-29	0.12650518092116414
1107	30-31	0.08894543229540375
1107	32-33	0.1351890322372995
1107	34-35	0.07581671690474678
1107	36-37	0.001708866658709951
1107	38-39	0.0906892820974079
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2216	36-37	-0.6032288670760906
2216	38-39	-0.5827677244471658
2216	40-41	-0.5860908203165209
2216	42-43	-0.5993339576601144
2216	44-45	-0.5707094033570428
2216	46-47	-0.5371254251390454
2216	48-49	-0.4426785687189181
2216	50-51	-0.5085795608459023
2216	52-53	-0.5120986326661381
2216	54-55	-0.45251433371787897
2216	56-57	-0.4923228094721068
2216	58-59	-0.489265750762371
2216	60-61	-0.4598956169632302
2216	62-63	-0.48581146103149564
2216	64-65	-0.4026657003653611
2216	66-67	-0.4517157101902072
2216	68-69	-0.34687083079395364
2216	70-71	-0.3975324193848735
2216	72-73	-0.356394688308022
2216	74-75	-0.35140086038162366
2216	76-77	-0.3682173000734821
2216	78-79	-0.288727245564381
2216	80-81	-0.3350850090185986
2216	82-83	-0.34557822496469015
2216	84-85	-0.3423221226145685
2216	86-87	-0.2806988412647087
2216	88-89	-0.2681743265779204
2216	90-91	-0.32258670226324426
2216	92-93	-0.31850550654896637
2216	94-95	-0.2946364631189127
2216	96-97	-0.27033247625519863
2216	98-99	-0.2677731332523905
2216	100	-0.337790300124972
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	11.0
8	116.0
9	258.0
10	292.0
11	185.0
12	225.0
13	265.0
14	429.0
15	835.0
16	1448.0
17	2331.0
18	4024.0
19	6132.0
20	8968.0
21	13133.0
22	18682.0
23	21936.0
24	21453.0
25	19359.0
26	18217.0
27	19768.0
28	23603.0
29	29251.0
30	36303.0
31	45611.0
32	57930.0
33	75620.0
34	107972.0
35	167338.0
36	297570.0
37	658327.0
38	912093.0
39	298241.0
40	654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96325631612139	19.425356798848732	13.877809707048266	23.73357717798161
2	15.273484971556503	20.991781750284296	39.798131005328926	23.936602272830275
3	18.123062410386755	26.34473468901131	30.95050263597596	24.58170026462598
4	12.362763268862269	18.011032650814787	38.612952482730094	31.01325159759285
5	12.647087737744053	38.26163681277423	35.15572109159896	13.93555435788276
6	29.578523411318187	38.69577540306429	17.689717454957584	14.03598373065994
7	25.521024339568704	33.91733889241367	22.797446820378024	17.7641899476396
8	25.415250600560768	35.10063728639611	20.490774319878657	18.993337793164468
9	26.13147485218291	15.420283004233108	20.67877510084261	37.769467042741375
10-11	24.44834959809299	26.312777707669387	28.464526276819075	20.77434641741855
12-13	25.701984954228223	24.31298761059479	28.375903757259692	21.609123677917296
14-15	22.717972905748805	25.18581499759986	26.966365576823925	25.129846519827414
16-17	21.518897542820927	28.23850805313023	27.507622254123476	22.734972149925365
18-19	21.491224477096733	26.93146824965911	30.066090294642763	21.5112169786014
20-21	22.492828760188196	25.932566204875485	30.00981497268767	21.564790062248644
22-23	22.27900215437603	25.78829943735228	29.95400163146923	21.978696776802458
24-25	22.136876085031616	26.06061187068166	29.979833227589957	21.822678816696765
26-27	21.90646160324844	26.111178508082062	29.990246031480428	21.99211385718907
28-29	21.712066597410566	26.435780072370303	29.936710846481535	21.9154424837376
30-31	22.12375297512443	26.103809292236306	29.71912036540759	22.053317367231667
32-33	22.112187002856643	26.172859604755182	29.53787357597052	22.177079816417656
34-35	22.39514002681819	26.186377175709687	29.709327852796623	21.7091549446755
36-37	22.37481038082102	25.90206264182765	29.68125153277137	22.041875444579954
38-39	22.30074202697066	26.17782466926367	29.569019175639077	21.952414128126588
40-41	22.295596075600827	26.11281627949012	29.488056142327533	22.103531502581518
42-43	22.297513055239875	26.083567479380044	29.10225616855727	22.516663296822816
44-45	22.38720587666246	26.205697827521547	29.068660414764587	22.33843588105141
46-47	22.77377657238076	26.30318833708664	28.61189159793347	22.311143492599125
48-49	22.74372925256508	26.51531987544349	28.25740284165235	22.48354803033908
50-51	22.461792942849772	26.832701197107976	28.126843246484324	22.578662613557928
52-53	22.689164539443468	26.883296279414726	27.77879551378868	22.64874366735313
54-55	22.427903524735935	26.713855111406577	27.930071068523876	22.928170295333615
56-57	22.630053158761296	26.688127946365043	27.877811890703995	22.804007004169662
58-59	22.724337292528347	26.615582865865022	27.943446777810916	22.716633063795722
60-61	22.411343240500557	26.679752196483047	28.24551874928318	22.663385813733218
62-63	22.370693178789676	26.629724973153042	28.17582391532798	22.823757932729304
64-65	22.11779351422599	26.702550974431343	28.069259584172674	23.110395927169996
66-67	22.28464266061434	26.64532712715752	27.9601595306045	23.109870681623633
68-69	22.252762672175685	26.574938408769157	28.054609554998784	23.11768936405638
70-71	22.334993792886344	26.391162157837435	27.96819782442497	23.305646224851255
72-73	22.32777540622046	26.407053478220476	28.08203144133028	23.183139674228787
74-75	22.255691075262387	26.829450677173416	27.813935798253063	23.10092244931113
76-77	22.411046221381817	26.494201061476975	27.727338331144004	23.367414385997208
78-79	22.31068377803974	26.521849700222116	27.786081458497797	23.381385063240348
80-81	22.339819361729905	26.61026884483993	27.728342241142347	23.32156955228782
82-83	22.468266782311076	26.40450525149609	27.999815935746238	23.1274120304466
84-85	22.340773582047746	26.63163055946624	27.671872115826574	23.355723742659436
86-87	22.10295686367471	26.802076288616668	27.626107690913276	23.46885915679535
88-89	22.215521268362746	26.469803875088022	27.49083170070209	23.823843155847143
90-91	22.185454127129102	26.42791904008255	27.58875471487635	23.797872117911997
92-93	22.39645691861451	26.49368075071381	27.500922495879053	23.608939834792622
94-95	22.001197108811404	26.310736539149364	27.80279985414376	23.885266497895476
96-97	21.95180874094061	26.204467731308632	27.927765402273607	23.915958125477154
98-99	21.892862191403996	26.388471773369126	27.596161371328254	24.122504663898628
100	22.03688495708593	25.927827894388344	27.564814162305257	24.47047298622047
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	135.5
2	386.0
3	666.5
4	423.0
5	306.0
6	759.5
7	2002.5
8	3798.5
9	4659.5
10	3395.5
11	1968.0
12	1764.5
13	1889.0
14	2185.0
15	2475.0
16	2804.5
17	3177.5
18	3611.5
19	4147.0
20	4747.5
21	5502.0
22	6608.5
23	8188.5
24	10072.0
25	12351.0
26	15521.5
27	19539.0
28	24090.0
29	29076.5
30	34870.0
31	41514.0
32	48501.0
33	53335.0
34	56619.5
35	61027.0
36	66720.0
37	73059.5
38	76547.0
39	77504.5
40	79896.0
41	82498.5
42	85375.5
43	89783.5
44	94794.5
45	101670.5
46	107318.0
47	113493.0
48	121332.0
49	119050.0
50	109839.0
51	105448.0
52	103065.5
53	100223.5
54	97963.0
55	91811.0
56	83774.0
57	75428.0
58	66510.5
59	58641.5
60	48653.0
61	39321.5
62	31639.0
63	24209.5
64	18163.5
65	13464.0
66	10209.0
67	7718.5
68	5506.5
69	3951.5
70	2955.5
71	2211.5
72	1647.0
73	1314.0
74	1065.5
75	814.0
76	572.0
77	403.0
78	271.5
79	162.5
80	125.5
81	89.5
82	59.5
83	33.5
84	19.0
85	14.0
86	10.5
87	7.5
88	6.0
89	2.0
90	0.5
91	2.0
92	2.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00303285946356734
2	0.0029979990099631175
3	1.0458136081266689E-4
4	0.050687099540539224
5	0.001882464494628004
6	0.004078673071694009
7	0.0
8	3.834649896464453E-4
9	3.48604536042223E-5
10-11	2.0916272162533378E-4
12-13	0.0
14-15	3.48604536042223E-5
16-17	2.0916272162533378E-4
18-19	1.5687204121900034E-4
20-21	1.2201158761477805E-4
22-23	0.0
24-25	0.0
26-27	1.394418144168892E-4
28-29	0.0
30-31	8.715113401055575E-5
32-33	1.2201158761477805E-4
34-35	1.5687204121900034E-4
36-37	5.2290680406333444E-5
38-39	6.449183916781125E-4
40-41	4.70616123657001E-4
42-43	0.0
44-45	4.531858968548899E-4
46-47	0.0
48-49	8.715113401055575E-5
50-51	0.0
52-53	4.3575567005277874E-4
54-55	3.6603476284433414E-4
56-57	1.0458136081266689E-4
58-59	8.889415669076686E-4
60-61	5.926277112717791E-4
62-63	1.9173249482322264E-4
64-65	3.3117430924011187E-4
66-67	0.0013769879173667807
68-69	0.0024750922058997833
70-71	0.003381463999609563
72-73	0.003294312865599007
74-75	0.0026319642471187834
76-77	0.004322696246923565
78-79	0.0013769879173667807
80-81	0.0010283833813245578
82-83	5.75197484469668E-4
84-85	5.403370308654456E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.2201158761477805E-4
94-95	6.97209072084446E-5
96-97	3.48604536042223E-4
98-99	1.2201158761477805E-4
100	0.07508941706349484
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2868580.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.70910183602963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.92423268067155	40.92973766411135
2	15.91662281462577	18.37068013789822
3	5.916563316597272	10.243186648704874
4	2.851120272292438	6.581423605619712
5	1.5087550276562125	4.353444876831703
6	0.8640773604853519	2.9919077034274113
7	0.5452437748494682	2.2025869969774416
8	0.3543860505084053	1.6361040542446323
9	0.24649336016267323	1.2802419381179553
>10	0.8434315519032907	7.46313436049716
>50	0.01951156921338766	0.7769131518074626
>100	0.007896138144860856	0.8467887499713987
>500	0.0010522628774734773	0.42295063878632894
>1k	3.507542924911568E-4	0.45361683729129376
>5k	8.76885731227892E-5	0.3005894704942308
>10k+	1.753771462455784E-4	1.1466931652187322
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11998	0.4182557223434591	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10662	0.3716821563282181	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5940	0.20707109440908045	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4536	0.15812701754875236	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.394418144168892E-4
2	0.0	0.0	0.0	0.0	1.394418144168892E-4
3	0.0	0.0	0.0	0.0	1.394418144168892E-4
4	0.0	0.0	0.0	3.48604536042223E-5	1.394418144168892E-4
5	0.0	0.0	0.0	3.48604536042223E-5	1.743022680211115E-4
6	0.0	0.0	0.0	3.48604536042223E-5	2.4402317522955608E-4
7	0.0	0.0	0.0	3.48604536042223E-5	2.4402317522955608E-4
8	0.0	0.0	0.0	6.97209072084446E-5	2.4402317522955608E-4
9	0.0	0.0	0.0	3.137440824380007E-4	2.4402317522955608E-4
10-11	0.0	0.0	0.0	3.137440824380007E-4	2.788836288337784E-4
12-13	0.0	0.0	0.0	3.137440824380007E-4	8.889415669076686E-4
14-15	0.0	0.0	0.0	3.6603476284433414E-4	0.0011155345153351136
16-17	0.0	0.0	0.0	4.1832544325066755E-4	0.0013246972369604473
18-19	0.0	0.0	0.0	4.1832544325066755E-4	0.0014641390513773366
20-21	0.0	0.0	0.0	4.70616123657001E-4	0.0016384413193984478
22-23	0.0	0.0	0.0	5.92627711271779E-4	0.0018127435874195595
24-25	0.0	0.0	0.0	9.935229277203356E-4	0.001847604041023782
26-27	0.0	0.0	0.0	0.0014467088245752255	0.0019521854018364486
28-29	0.0	0.0	0.0	0.003712638308849675	0.0020219063090448934
30-31	0.0	0.0	0.0	0.010196682679235023	0.0020393365358470045
32-33	0.0	0.0	0.0	0.027539758347335616	0.00212648766985756
34-35	0.0	0.0	0.0	0.05328420333405379	0.0021613481234617825
36-37	0.0	0.0	0.0	0.09602311945283032	0.0021613481234617825
38-39	0.0	0.0	0.0	0.16185708608440413	0.0022136388038681157
40-41	0.0	0.0	0.0	0.2391950024053713	0.0022310690306702272
42-43	0.0	0.0	0.0	0.3141798381080535	0.0022310690306702272
44-45	0.0	0.0	0.0	0.40952317871560145	0.0023879410718892274
46-47	0.0	0.0	0.0	0.5330163356085589	0.0025796735667124497
48-49	0.0	0.0	0.0	0.667734558562076	0.002788836288337784
50-51	0.0	0.0	0.0	0.8123357201123902	0.0029631385563588953
52-53	0.0	0.0	0.0	0.9530673713126355	0.0033466035460053404
54-55	0.0	0.0	0.0	1.1099394125316357	0.003416324453213785
56-57	0.0	0.0	0.0	1.3016021864476501	0.0034860453604222295
58-59	0.0	0.0	0.0	1.5179984521958598	0.003520905814026452
60-61	0.0	0.0	0.0	1.7578732334465137	0.003520905814026452
62-63	0.0	0.0	0.0	1.996440747687009	0.003660347628443341
64-65	0.0	0.0	0.0	2.2425381199060164	0.003852080123266564
66-67	0.0	0.0	0.0	2.52196208577066	0.003886940576870786
68-69	0.0	0.0	0.0	2.8457285486198747	0.0039043708036728972
70-71	0.0	0.0	0.0	3.2026821633003086	0.003991521937683453
72-73	0.0	0.0	0.0	3.5612567890733393	0.004235545112913009
74-75	0.0	0.0	0.0	3.9193433684959107	0.0045841496489552325
76-77	0.0	0.0	0.0	4.308002565729385	0.004880463504591122
78-79	0.0	0.0	0.0	4.753588883698555	0.004897893731393233
80-81	0.0	0.0	0.0	5.237155665869524	0.004915323958195344
82-83	0.0	0.0	0.0	5.735328978100663	0.005211637813831233
84-85	0.0	0.0	0.0	6.2285695361468045	0.005298788947841789
86-87	0.0	0.0	0.0	6.723291663471125	0.005490521442665012
88	0.0	0.0	0.0	7.116552440580357	0.005682253937488235
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11005	0.0	33.758915	1
GTATCAA	16645	0.0	31.784853	1
ATCAACG	19845	0.0	26.326872	3
TATCAAC	20070	0.0	26.312899	2
CAACGCA	19845	0.0	26.288044	5
TCAACGC	20045	0.0	26.087654	4
AACGCAG	20405	0.0	25.70389	6
GAGTACT	13235	0.0	23.329891	12-13
ACGCAGA	22390	0.0	23.257174	7
CGCAGAG	22700	0.0	22.85675	8
GTACATG	8900	0.0	22.50977	1
TACATGG	8810	0.0	22.20515	2
AGAGTAC	17525	0.0	22.164406	10-11
GTACTTT	14080	0.0	21.612669	14-15
GCAGAGT	23900	0.0	21.296186	9
ACATGGG	8640	0.0	21.281357	3
AGTACTT	13900	0.0	20.776785	12-13
GTACTAG	875	0.0	18.810982	1
ACTTTTT	16175	0.0	18.711683	16-17
TACTTTT	15365	0.0	18.505213	14-15
>>END_MODULE