FastQCFastQC Report
Mon 16 Jan 2017
ERR1146524_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146524_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265359
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41600.034.0281331
GTATCAA65850.031.3526121
ATCAACG65100.029.2455083
CAACGCA67700.027.981755
AACGCAG76250.026.1311196
GTACATG112700.026.080811
TATCAAC78650.025.5817452
TACATGG113100.024.9386982
TCAACGC79000.024.277184
CGCAGAG78950.023.5102358
ACGCAGA80300.023.4082957
ACATGGG112450.023.201643
CGTTAAC4350.022.7036151
TAGACCG1703.4508757E-622.1207355
CATGGGG75350.022.0843754
GAGTACA82500.020.9207421
GTGATCG8150.020.7566348
GCAGAGT86650.020.6072679
CTCGTCG11100.020.3199969
CTGTGCG19600.019.8988239
Produced by FastQC (version 0.11.5)