##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146524_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3265359
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21646532586463	31.0	31.0	34.0	28.0	34.0
2	31.38539284654459	31.0	31.0	34.0	28.0	34.0
3	31.483414227960846	33.0	31.0	34.0	28.0	34.0
4	34.936701293793426	37.0	35.0	37.0	32.0	37.0
5	34.99950265805383	37.0	35.0	37.0	32.0	37.0
6	35.146731492616894	37.0	35.0	37.0	32.0	37.0
7	35.09546362283596	37.0	35.0	37.0	32.0	37.0
8	35.04959607810351	37.0	35.0	37.0	32.0	37.0
9	36.65047549136251	39.0	37.0	39.0	32.0	39.0
10-11	36.636526642246686	39.0	37.0	39.0	32.0	39.0
12-13	36.58094393296419	39.0	37.0	39.0	32.0	39.0
14-15	37.94592799750349	40.0	38.0	41.0	32.0	41.0
16-17	37.983830414971216	40.0	38.0	41.0	32.0	41.0
18-19	38.02503691018353	40.0	38.0	41.0	32.0	41.0
20-21	38.12918457051737	40.0	38.0	41.0	32.0	41.0
22-23	38.15739846675358	40.0	38.0	41.0	32.0	41.0
24-25	38.19198042236704	40.0	38.0	41.0	32.0	41.0
26-27	38.262748291994846	40.0	38.0	41.0	33.0	41.0
28-29	38.24604492186005	40.0	38.0	41.0	32.5	41.0
30-31	38.21316966985866	40.0	38.0	41.0	32.0	41.0
32-33	38.17837778326977	40.0	38.0	41.0	32.0	41.0
34-35	38.12755718437084	40.0	38.0	41.0	32.0	41.0
36-37	38.052549964644015	40.0	38.0	41.0	32.0	41.0
38-39	37.99054912492011	40.0	38.0	41.0	32.0	41.0
40-41	37.94287810314272	40.0	38.0	41.0	32.0	41.0
42-43	37.78876059263315	40.0	38.0	41.0	31.0	41.0
44-45	37.68354184026933	40.0	37.0	41.0	31.0	41.0
46-47	37.5784861633897	40.0	37.0	41.0	31.0	41.0
48-49	37.470837050382514	40.0	37.0	41.0	31.0	41.0
50-51	37.341315150952774	40.0	36.5	41.0	30.5	41.0
52-53	37.211165142944466	40.0	36.0	41.0	30.0	41.0
54-55	37.090939771094085	40.0	36.0	41.0	30.0	41.0
56-57	36.904032604072015	39.0	35.0	41.0	30.0	41.0
58-59	36.70663975997739	39.0	35.0	41.0	30.0	41.0
60-61	36.49486442378924	39.0	35.0	41.0	30.0	41.0
62-63	36.26198711994608	38.0	35.0	40.5	30.0	41.0
64-65	35.976202463496364	37.5	35.0	40.0	29.5	41.0
66-67	35.739383785978816	37.0	35.0	40.0	29.0	41.0
68-69	35.48098049862205	37.0	35.0	40.0	29.0	41.0
70-71	35.204159481392395	36.0	34.0	39.0	29.0	41.0
72-73	34.869091423025765	36.0	34.0	39.0	29.0	41.0
74-75	34.53634776451839	35.0	34.0	38.5	28.5	40.0
76-77	34.25902083048143	35.0	34.0	37.0	28.0	39.5
78-79	33.9411243909169	35.0	34.0	37.0	28.0	39.0
80-81	33.69673502974711	35.0	34.0	36.5	28.0	39.0
82-83	33.416169248159235	35.0	34.0	36.0	27.5	37.5
84-85	33.20883967122757	35.0	34.0	36.0	27.0	37.0
86-87	33.00410169295321	35.0	33.5	35.0	27.0	37.0
88-89	32.83297119857265	35.0	33.0	35.0	27.0	36.0
90-91	32.693533697213695	35.0	33.0	35.0	27.0	36.0
92-93	32.54727902812524	35.0	33.0	35.0	26.5	36.0
94-95	32.43841611289908	35.0	33.0	35.0	26.0	36.0
96-97	32.35850085702675	35.0	33.0	35.0	26.0	35.0
98-99	32.27069336020939	35.0	33.0	35.0	25.0	35.0
100	31.156208551647765	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22746578841575626
1101	2	0.31183512510251177
1101	3	0.26691334434507397
1101	4	0.2874936233158749
1101	5	0.21242512366942634
1101	6	0.2672050403164121
1101	7	0.2782058909147409
1101	8	0.314574779173995
1101	9	0.4397600826611594
1101	10-11	0.4143834013734775
1101	12-13	0.3882571965756654
1101	14-15	0.4818860912284393
1101	16-17	0.45593217191174773
1101	18-19	0.3873445143354317
1101	20-21	0.45755078877007094
1101	22-23	0.4550089201908847
1101	24-25	0.4651153765243379
1101	26-27	0.4361926273453136
1101	28-29	0.4717500784884123
1101	30-31	0.5106204995825152
1101	32-33	0.5536502711111027
1101	34-35	0.4553577422353712
1101	36-37	0.4675566610394668
1101	38-39	0.5174224794227982
1101	40-41	0.47550631126933496
1101	42-43	0.5101687778689552
1101	44-45	0.4611940764098108
1101	46-47	0.45019760696082756
1101	48-49	0.5018620954686597
1101	50-51	0.44002104109895157
1101	52-53	0.4166077356068598
1101	54-55	0.4485668656902817
1101	56-57	0.4402437594009214
1101	58-59	0.3671252430774672
1101	60-61	0.42883359468309834
1101	62-63	0.47518186364211346
1101	64-65	0.5233087799588247
1101	66-67	0.4275864862156453
1101	68-69	0.46115828780732926
1101	70-71	0.41508975738579323
1101	72-73	0.4740575544042116
1101	74-75	0.42486542465571375
1101	76-77	0.47467385503171045
1101	78-79	0.41834774490332904
1101	80-81	0.33785410384963654
1101	82-83	0.34539124540583543
1101	84-85	0.3152023773452086
1101	86-87	0.29922649731533824
1101	88-89	0.3112214182410895
1101	90-91	0.2732592540277423
1101	92-93	0.28872321426769076
1101	94-95	0.2827282246537237
1101	96-97	0.29330368339590507
1101	98-99	0.29751701903206396
1101	100	0.2340208351386046
1102	1	0.2199571436914134
1102	2	0.27492281638315674
1102	3	0.30876579609504873
1102	4	0.28297990085663827
1102	5	0.33535795467523855
1102	6	0.3467532148408452
1102	7	0.3611079648190625
1102	8	0.35212154928761663
1102	9	0.44799070898726256
1102	10-11	0.4488933825842949
1102	12-13	0.44796503296433343
1102	14-15	0.5543459103765827
1102	16-17	0.5266398437191455
1102	18-19	0.4861958837441662
1102	20-21	0.45240777437937396
1102	22-23	0.44544908071352296
1102	24-25	0.5615161500683286
1102	26-27	0.4963780379431171
1102	28-29	0.4854996172941881
1102	30-31	0.5040948041665416
1102	32-33	0.48621880646644655
1102	34-35	0.4714467935656117
1102	36-37	0.44209729716470747
1102	38-39	0.4830507512870099
1102	40-41	0.37680058846964215
1102	42-43	0.45018659929142757
1102	44-45	0.3751166599774791
1102	46-47	0.4030157396090033
1102	48-49	0.3153495905982524
1102	50-51	0.33682735298899047
1102	52-53	0.279771063341542
1102	54-55	0.19857221548318194
1102	56-57	0.307836926044331
1102	58-59	0.34457685780726166
1102	60-61	0.32156438625167283
1102	62-63	0.331526128946642
1102	64-65	0.41546954808809744
1102	66-67	0.3638445453179955
1102	68-69	0.3309066996702157
1102	70-71	0.2796396131878396
1102	72-73	0.25802771746710107
1102	74-75	0.30292023154080994
1102	76-77	0.22356900257719303
1102	78-79	0.21332901401606819
1102	80-81	0.20309011567399438
1102	82-83	0.1574098016133476
1102	84-85	0.20218508693476167
1102	86-87	0.23243754880932244
1102	88-89	0.2366434117085774
1102	90-91	0.15198365662117652
1102	92-93	0.2580791797496289
1102	94-95	0.2997625663569039
1102	96-97	0.26724995151862885
1102	98-99	0.32637951240342744
1102	100	0.4222991623821919
1103	1	0.1765325301199816
1103	2	0.20721591751802038
1103	3	0.2208957180129012
1103	4	0.23880671557414956
1103	5	0.22748830031292044
1103	6	0.3100747703320579
1103	7	0.3450395670214661
1103	8	0.32445421330054813
1103	9	0.434276310281021
1103	10-11	0.41573985554821746
1103	12-13	0.453256822921702
1103	14-15	0.5167709845471791
1103	16-17	0.4869023773911394
1103	18-19	0.4645932340827841
1103	20-21	0.43452444328233497
1103	22-23	0.4704026480814463
1103	24-25	0.4587540852344887
1103	26-27	0.44977277926608394
1103	28-29	0.42585928481535973
1103	30-31	0.4399136074404879
1103	32-33	0.4762490318147812
1103	34-35	0.48637585580299714
1103	36-37	0.48713636177962627
1103	38-39	0.4531261263379136
1103	40-41	0.41127568982891916
1103	42-43	0.454669398227729
1103	44-45	0.3840612680248654
1103	46-47	0.4042967074478838
1103	48-49	0.38669361422612525
1103	50-51	0.4066412772074841
1103	52-53	0.3359643564842685
1103	54-55	0.33793975085398387
1103	56-57	0.40520774445919017
1103	58-59	0.36717230658542377
1103	60-61	0.3775540584729953
1103	62-63	0.3513944697774676
1103	64-65	0.28537676913724397
1103	66-67	0.2956048579918473
1103	68-69	0.316391548939734
1103	70-71	0.25868627494249097
1103	72-73	0.23201622367420782
1103	74-75	0.23858759420654252
1103	76-77	0.23627196634051728
1103	78-79	0.22002614143097787
1103	80-81	0.20586979626771296
1103	82-83	0.30223057204585757
1103	84-85	0.23170364704060376
1103	86-87	0.20295782968849352
1103	88-89	0.23045965226216936
1103	90-91	0.25328329951951645
1103	92-93	0.27936992453139453
1103	94-95	0.2173749521585222
1103	96-97	0.28681756896552457
1103	98-99	0.28745221409604227
1103	100	0.34930400455832
1104	1	0.20624299066691876
1104	2	0.19417643632642978
1104	3	0.16095368483466288
1104	4	0.18502630910994355
1104	5	0.15576796611973265
1104	6	0.30313841329500235
1104	7	0.25515981335367144
1104	8	0.2299517395316002
1104	9	0.35792950582261085
1104	10-11	0.33709792389228
1104	12-13	0.32680913904982134
1104	14-15	0.4266829092653097
1104	16-17	0.39786258011429254
1104	18-19	0.4472557434247122
1104	20-21	0.4339667135245193
1104	22-23	0.39657611459917774
1104	24-25	0.4624844537952555
1104	26-27	0.45138492122586626
1104	28-29	0.4514602084271928
1104	30-31	0.4573908184970463
1104	32-33	0.4703328533391087
1104	34-35	0.43362920885147105
1104	36-37	0.4129008996924526
1104	38-39	0.3521501193518972
1104	40-41	0.4099421850595135
1104	42-43	0.4226474972695655
1104	44-45	0.3910439128012797
1104	46-47	0.35171222830549453
1104	48-49	0.33454731496838974
1104	50-51	0.37772483354164876
1104	52-53	0.428306333945784
1104	54-55	0.3464946251297363
1104	56-57	0.4037855750964212
1104	58-59	0.36885610346582354
1104	60-61	0.3703031698180439
1104	62-63	0.39918747143557454
1104	64-65	0.4983676589172461
1104	66-67	0.3994261412444189
1104	68-69	0.3722131964939521
1104	70-71	0.4101678844057659
1104	72-73	0.4662437032692921
1104	74-75	0.3764119633084064
1104	76-77	0.39542383994927377
1104	78-79	0.42283739835203704
1104	80-81	0.3459759526977635
1104	82-83	0.4291759026897921
1104	84-85	0.4184447276771692
1104	86-87	0.30179367161371573
1104	88-89	0.3484096786472577
1104	90-91	0.4286035501376233
1104	92-93	0.3702118363650442
1104	94-95	0.35296652714706767
1104	96-97	0.3912396017151778
1104	98-99	0.3872133018579049
1104	100	0.49084869713862034
1105	1	0.2741545066070614
1105	2	0.2699474187597417
1105	3	0.2638684538652427
1105	4	0.2697411215150609
1105	5	0.20106399734940084
1105	6	0.2801889446331103
1105	7	0.30484968539877855
1105	8	0.31790229255697966
1105	9	0.38015461968077346
1105	10-11	0.3645778631679235
1105	12-13	0.37339556281906283
1105	14-15	0.4266894154461838
1105	16-17	0.43523191431512487
1105	18-19	0.4430354007658508
1105	20-21	0.40272186425104195
1105	22-23	0.36948871490280055
1105	24-25	0.4069368502385373
1105	26-27	0.36632311250758676
1105	28-29	0.42628562590712704
1105	30-31	0.4658878907156492
1105	32-33	0.43674577895178146
1105	34-35	0.4187213797471614
1105	36-37	0.42480287323397903
1105	38-39	0.463726783849836
1105	40-41	0.4140757786400755
1105	42-43	0.4066119301474629
1105	44-45	0.4133991502768879
1105	46-47	0.4258696778825879
1105	48-49	0.38189062444572386
1105	50-51	0.34465174744318716
1105	52-53	0.40230546645499743
1105	54-55	0.34542327401283757
1105	56-57	0.3754533261265678
1105	58-59	0.3053189097985012
1105	60-61	0.40978267534829627
1105	62-63	0.3560254675318646
1105	64-65	0.39048216770058986
1105	66-67	0.3743231267139464
1105	68-69	0.37717741249915804
1105	70-71	0.4162880318791977
1105	72-73	0.40415305048247774
1105	74-75	0.327520182783573
1105	76-77	0.3387550045556509
1105	78-79	0.3475023300371447
1105	80-81	0.307626353912255
1105	82-83	0.28505315274401255
1105	84-85	0.34504199508120337
1105	86-87	0.21606389499259393
1105	88-89	0.29467946828074787
1105	90-91	0.2950121382963715
1105	92-93	0.25193163684215847
1105	94-95	0.2546971712589752
1105	96-97	0.27376833734735584
1105	98-99	0.2627652428685394
1105	100	0.32118484981700135
1106	1	0.06971901895361299
1106	2	0.09240176523718446
1106	3	0.10900930841086875
1106	4	0.16154404765835295
1106	5	0.11034288653556956
1106	6	0.16116279684241874
1106	7	0.2002631429025783
1106	8	0.240034774192452
1106	9	0.2784999888186874
1106	10-11	0.26818012004404324
1106	12-13	0.25024309347350737
1106	14-15	0.3430471190261528
1106	16-17	0.3078729687224495
1106	18-19	0.26972051491243576
1106	20-21	0.27953033261877636
1106	22-23	0.23418584427275846
1106	24-25	0.30446044126955485
1106	26-27	0.23954443088356214
1106	28-29	0.24472215718166979
1106	30-31	0.28718415939921726
1106	32-33	0.30671813713362184
1106	34-35	0.3239001289111343
1106	36-37	0.30180616300656027
1106	38-39	0.2120103183679518
1106	40-41	0.23845208762920578
1106	42-43	0.26888481760229865
1106	44-45	0.27927094841194844
1106	46-47	0.2864830481892895
1106	48-49	0.2306196790445867
1106	50-51	0.24292893258960646
1106	52-53	0.2353537431011148
1106	54-55	0.19986298291340177
1106	56-57	0.1717625420680733
1106	58-59	0.20808489771943783
1106	60-61	0.19398118232889772
1106	62-63	0.20799137161588988
1106	64-65	0.24964820328031578
1106	66-67	0.2479620895388308
1106	68-69	0.2678178505904043
1106	70-71	0.19588621955710295
1106	72-73	0.17328569550751638
1106	74-75	0.1654451549114455
1106	76-77	0.20180318739627978
1106	78-79	0.07359309539054237
1106	80-81	0.16830188785801425
1106	82-83	0.19361681006050446
1106	84-85	0.1771568370840555
1106	86-87	0.14445894323871755
1106	88-89	0.20933033194229722
1106	90-91	0.23734059220407033
1106	92-93	0.16472984204909125
1106	94-95	0.13841609416471812
1106	96-97	0.17826734671936606
1106	98-99	0.16717968729011545
1106	100	0.24133243525665193
1107	1	0.10933888585327622
1107	2	0.22223442081354605
1107	3	0.15789768545440452
1107	4	0.15397613916542952
1107	5	0.12927589202198675
1107	6	0.19313657343987245
1107	7	0.22647240474120878
1107	8	0.27162842856703406
1107	9	0.2674562757148351
1107	10-11	0.3153667170441423
1107	12-13	0.314045047211998
1107	14-15	0.3247754636620783
1107	16-17	0.3118807268960069
1107	18-19	0.28970759247912525
1107	20-21	0.4055854471231797
1107	22-23	0.42117957740020984
1107	24-25	0.431491174108217
1107	26-27	0.35739974658487483
1107	28-29	0.31541797891010503
1107	30-31	0.35263982836448093
1107	32-33	0.3356847014251727
1107	34-35	0.30291878268292294
1107	36-37	0.38709107292933
1107	38-39	0.3505503653114772
1107	40-41	0.35465081010671895
1107	42-43	0.3667182251743526
1107	44-45	0.3292073016207695
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2216	42-43	-0.6421276667404143
2216	44-45	-0.5186993641451494
2216	46-47	-0.4770967339586605
2216	48-49	-0.49616423079175576
2216	50-51	-0.5726003060701501
2216	52-53	-0.5702626790123588
2216	54-55	-0.604418984987376
2216	56-57	-0.5359169026741242
2216	58-59	-0.5925958931184354
2216	60-61	-0.5490157482582987
2216	62-63	-0.5720880111061817
2216	64-65	-0.5727468973069989
2216	66-67	-0.4637314235462995
2216	68-69	-0.5308058747487294
2216	70-71	-0.5112612388863624
2216	72-73	-0.45755841395039454
2216	74-75	-0.37190103820970677
2216	76-77	-0.41404570299151544
2216	78-79	-0.4388763703532277
2216	80-81	-0.44403746630027996
2216	82-83	-0.44708365326336263
2216	84-85	-0.4447134809543627
2216	86-87	-0.42987533321170446
2216	88-89	-0.418185318370746
2216	90-91	-0.4059992000792505
2216	92-93	-0.39309181251806535
2216	94-95	-0.4422850790663375
2216	96-97	-0.38125497646743156
2216	98-99	-0.3920776260861345
2216	100	-0.36793322381128135
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	977.0
1	261.0
2	192.0
3	161.0
4	115.0
5	184.0
6	273.0
7	719.0
8	2055.0
9	2669.0
10	2461.0
11	2713.0
12	3523.0
13	4897.0
14	7818.0
15	9013.0
16	9446.0
17	10225.0
18	11018.0
19	12043.0
20	13381.0
21	14635.0
22	16297.0
23	18612.0
24	22923.0
25	28602.0
26	31090.0
27	28442.0
28	30367.0
29	37669.0
30	44039.0
31	49443.0
32	62552.0
33	83508.0
34	125117.0
35	194383.0
36	360741.0
37	759215.0
38	945320.0
39	317722.0
40	538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.752115751948374	21.002250405339662	13.280978173735217	23.964655668976746
2	15.053500492301977	21.303728409884528	40.47598690994628	23.166784187867215
3	17.617098173880557	26.96941285942886	30.959377033393398	24.454111933297188
4	11.913376764486374	18.357370652861874	40.444640604909836	29.284611977741914
5	12.674743137931605	39.09510869398641	35.67733060834672	12.552817559735258
6	28.703762453565894	39.603282104475234	18.91222433415205	12.780731107806814
7	25.278841287974107	33.913335064102306	23.738221907673182	17.069601740250402
8	24.66014132284881	35.95775456488495	21.373908453715433	18.008195658550804
9	25.8580515454597	15.951203590621354	21.479491686191462	36.711253177727485
10-11	23.813579023395185	27.148928579583515	29.231328701077924	19.806163695943376
12-13	24.306773107384316	25.357665267050294	28.499017939422693	21.836543686142697
14-15	22.05589385170083	26.542576276007157	26.09608257558947	25.305447296702543
16-17	21.567128932212256	29.165332137595286	26.99105862968052	22.27648030051194
18-19	21.320636543589703	29.150497231676265	28.32622958582493	21.202636638909105
20-21	22.855569370385957	28.156551687573305	27.47645647454995	21.511422467490796
22-23	22.846828949554105	28.085603988155185	27.00101935049659	22.066547711794122
24-25	22.40435567415931	28.27750949076071	27.39671111600129	21.921423719078692
26-27	22.207859073607562	28.385494241460265	27.148189691631575	22.2584569933006
28-29	21.884115827470982	28.76130870238507	27.23557730987933	22.118998160264628
30-31	22.382274957736545	28.413868757778566	27.110525274472998	22.09333101001189
32-33	22.347689542481657	28.691095271928337	26.745019331717813	22.216195853872197
34-35	22.570934463936286	28.557068189286085	27.14677751185613	21.725219834921507
36-37	22.52754326322265	28.176644097482445	27.12829430240745	22.16751833688745
38-39	22.21963890188212	28.32264891113303	27.360361232988655	22.09735095399619
40-41	22.327610159888295	27.915738760179515	27.59285718773951	22.163793892192682
42-43	22.151192552562126	28.123595712266997	27.309790855002408	22.41542088016847
44-45	22.135825315999803	28.536511913930045	27.27950129694977	22.04816147312038
46-47	22.131570748276697	28.257748179993204	27.08457309474947	22.52610797698063
48-49	22.253348993106524	27.939284302589613	27.431848796848467	22.3755179074554
50-51	22.22663612491222	28.053094958066392	27.100381649322173	22.619887267699212
52-53	22.009373941484903	28.30934926515104	27.049124296686166	22.63215249667789
54-55	22.006729804303568	28.109392729874187	27.222941166013076	22.660936299809165
56-57	22.45978863023472	27.946926892668266	26.96982260174402	22.623461875352994
58-59	22.056966216083655	27.986210909144415	27.36280188734771	22.59402098742422
60-61	22.089838969273377	28.09249944094536	27.266263432063635	22.551398157717628
62-63	22.185154116278966	27.711134037273116	27.03273456404009	23.070977282407824
64-65	21.781781674381534	27.825342570736645	27.26807884491585	23.124796909965973
66-67	22.317660246186772	27.617893796680015	27.038914812545723	23.025531144587482
68-69	21.993442964675083	27.60979355608043	27.245799926122228	23.150963553122256
70-71	22.057557408345527	27.530638349073705	27.222243085056242	23.18956115752453
72-73	22.301883264283887	27.498540075502238	27.35673197625496	22.842844683958916
74-75	22.239820284156668	27.797847879477818	27.255702224238004	22.706629612127507
76-77	22.111188735945145	27.652907043222385	27.196901164852772	23.0390030559797
78-79	22.206154635213572	27.666949067196867	27.080667785538896	23.046228512050664
80-81	22.1408233697868	27.545065109204064	27.42644367451468	22.887667846494452
82-83	22.47071501053064	27.153232308678042	27.752482441269073	22.62357023952224
84-85	22.561632833240346	27.393548861762202	27.10946457146711	22.935353733530345
86-87	22.052057687376454	27.49362042652903	27.46929612333142	22.985025762763094
88-89	22.522238210177374	26.97854010418806	27.26666049492088	23.23256119071369
90-91	22.443437069208375	26.936897389860654	27.444919214643782	23.174746326287192
92-93	22.70230281231757	26.94196768280095	27.38970822073023	22.96602128415125
94-95	22.080878841436913	26.957156510602974	27.63203066712523	23.329933980834884
96-97	22.293174319986058	26.814482912619543	27.43718948772112	23.455153279673276
98-99	22.016434443938447	27.25959808895826	27.285594485943477	23.438372981159816
100	22.485319724249038	26.949050663221826	27.010755711861368	23.554873900667765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1853.0
1	1239.0
2	660.5
3	702.5
4	665.0
5	599.5
6	563.5
7	518.5
8	526.0
9	567.0
10	523.0
11	493.0
12	485.5
13	478.0
14	514.5
15	601.5
16	698.5
17	824.0
18	1060.5
19	1334.5
20	1732.5
21	2480.0
22	3703.0
23	5283.5
24	7338.5
25	10278.5
26	15507.5
27	21341.0
28	26219.5
29	32922.5
30	41914.0
31	50362.5
32	59868.5
33	68094.0
34	76723.5
35	85249.0
36	92288.0
37	102471.5
38	106100.0
39	102764.0
40	101578.0
41	103019.0
42	105474.0
43	109168.0
44	113303.0
45	121442.0
46	128205.5
47	133002.5
48	135639.5
49	132210.0
50	127910.5
51	125053.5
52	121423.5
53	116586.5
54	111086.5
55	99698.0
56	88215.5
57	74931.0
58	62092.5
59	55110.0
60	44224.0
61	32896.0
62	25661.0
63	19529.0
64	13859.5
65	8882.5
66	5946.0
67	4358.0
68	3270.0
69	2617.5
70	2225.5
71	1808.5
72	1531.0
73	1462.0
74	1227.0
75	913.5
76	724.5
77	574.0
78	421.0
79	321.5
80	264.5
81	203.5
82	159.0
83	112.5
84	84.0
85	63.5
86	44.5
87	40.0
88	28.0
89	20.5
90	20.5
91	15.5
92	11.5
93	11.5
94	10.5
95	9.0
96	6.0
97	5.0
98	7.5
99	8.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10044837336415384
2	0.06486269962965786
3	0.08109368678910957
4	0.08342114909876679
5	0.08308427955394797
6	0.09003604197884521
7	0.08697359157140148
8	0.08881106181586773
9	0.07392755283569126
10-11	0.08138461957781672
12-13	0.07111009846084304
14-15	0.07971558410575989
16-17	0.08146118083800281
18-19	0.08098650102484903
20-21	0.07227382961567165
22-23	0.07646938667386954
24-25	0.07902653276408506
26-27	0.07285569519308596
28-29	0.08150711759411446
30-31	0.08539642961156797
32-33	0.08712671409177368
34-35	0.08914793136068652
36-37	0.09021978900329183
38-39	0.09116914862959938
40-41	0.08766264291307634
42-43	0.09276162284147012
44-45	0.08870387605160719
46-47	0.0858251726686101
48-49	0.09409378876870812
50-51	0.09003604197884521
52-53	0.09567095072854163
54-55	0.08749420814066693
56-57	0.09391004174426151
58-59	0.09845778059931541
60-61	0.0966356226068864
62-63	0.09786060276986389
64-65	0.09980525877859064
66-67	0.10178053929139186
68-69	0.09842715609524098
70-71	0.1014130452424986
72-73	0.0981209110544966
74-75	0.10168866577916853
76-77	0.09891714816043198
78-79	0.10545547978032431
80-81	0.10109148794971701
82-83	0.10562391455273372
84-85	0.10103023894156814
86-87	0.09767685574541728
88-89	0.09741654746078456
90-91	0.09787591502190111
92-93	0.09121508538571103
94-95	0.09104665061330164
96-97	0.089040745596426
98-99	0.10315864197474152
100	0.24276044379806325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3265359.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.92622926536177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.65873134351291	33.946778349378945
2	15.698403765152616	15.98921018488761
3	6.808692905269677	10.40223167673617
4	3.6420496888352383	7.419034297978512
5	2.0746544166057057	5.2827163233228775
6	1.3520642429487943	4.1313320170744845
7	0.9088327875112604	3.239839883047338
8	0.6144707217146459	2.503414148071389
9	0.4616631296442373	2.1159686145264174
>10	1.7514103398670193	13.722311507631298
>50	0.02444874091714916	0.8194308372975553
>100	0.00446896698265005	0.32774881090012853
>500	0.0	0.0
>1k	1.0895103812911667E-4	0.09998334914725546
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.062450407443714E-5	0.0	0.0	0.0	0.0
7	3.062450407443714E-5	0.0	0.0	0.0	0.0
8	3.062450407443714E-5	0.0	0.0	0.0	0.0
9	3.062450407443714E-5	0.0	0.0	0.0	0.0
10-11	3.062450407443714E-5	0.0	0.0	0.0	0.0
12-13	3.062450407443714E-5	0.0	0.0	0.0	0.0
14-15	3.062450407443714E-5	0.0	0.0	1.531225203721857E-5	0.0
16-17	3.062450407443714E-5	0.0	0.0	4.593675611165571E-5	0.0
18-19	3.062450407443714E-5	0.0	0.0	9.187351222331143E-5	0.0
20-21	3.062450407443714E-5	0.0	0.0	1.3781026833496716E-4	0.0
22-23	3.062450407443714E-5	0.0	0.0	4.5936756111655716E-4	0.0
24-25	3.062450407443714E-5	0.0	0.0	0.0011943556589030487	0.0
26-27	3.062450407443714E-5	0.0	0.0	0.002373399065768879	0.0
28-29	3.062450407443714E-5	0.0	0.0	0.0076867505226837236	0.0
30-31	3.062450407443714E-5	0.0	0.0	0.02347368237305607	0.0
32-33	3.062450407443714E-5	0.0	0.0	0.05579784642362448	0.0
34-35	3.062450407443714E-5	0.0	0.0	0.10776762983794431	0.0
36-37	3.062450407443714E-5	0.0	0.0	0.1893053719361332	0.0
38-39	3.062450407443714E-5	0.0	0.0	0.31145120643702573	0.0
40-41	3.062450407443714E-5	0.0	0.0	0.44742400452752673	0.0
42-43	3.062450407443714E-5	0.0	0.0	0.586995181846774	0.0
44-45	3.062450407443714E-5	0.0	0.0	0.7569152427037884	0.0
46-47	3.062450407443714E-5	0.0	0.0	0.9612266216363958	0.0
48-49	3.062450407443714E-5	0.0	0.0	1.1904204101294833	0.0
50-51	3.062450407443714E-5	0.0	0.0	1.4350030119199757	0.0
52-53	3.062450407443714E-5	0.0	0.0	1.6739507049607716	0.0
54-55	3.062450407443714E-5	0.0	0.0	1.9357749025451718	0.0
56-57	3.062450407443714E-5	0.0	0.0	2.242356812834362	0.0
58-59	3.062450407443714E-5	0.0	0.0	2.586484365118812	0.0
60-61	3.062450407443714E-5	0.0	0.0	2.9531362401500107	0.0
62-63	3.062450407443714E-5	0.0	0.0	3.3261120752725812	0.0
64-65	3.062450407443714E-5	0.0	0.0	3.7141704786518113	0.0
66-67	4.593675611165571E-5	0.0	0.0	4.15101371702162	0.0
68-69	6.124900814887428E-5	0.0	0.0	4.639428620252781	0.0
70-71	6.124900814887428E-5	0.0	0.0	5.183472935135157	0.0
72-73	6.124900814887428E-5	0.0	0.0	5.7301203328638595	0.0
74-75	6.124900814887428E-5	0.0	0.0	6.275496813673474	0.0
76-77	6.124900814887428E-5	0.0	0.0	6.867223481399748	0.0
78-79	6.124900814887428E-5	0.0	0.0	7.512328659727766	0.0
80-81	6.124900814887428E-5	0.0	0.0	8.233459169420575	0.0
82-83	6.124900814887428E-5	0.0	0.0	8.968753512247812	0.0
84-85	6.124900814887428E-5	0.0	0.0	9.692686164063431	0.0
86-87	6.124900814887428E-5	0.0	0.0	10.434855708055377	0.0
88	6.124900814887428E-5	3.062450407443714E-5	0.0	11.023749609154766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4160	0.0	34.028133	1
GTATCAA	6585	0.0	31.352612	1
ATCAACG	6510	0.0	29.245508	3
CAACGCA	6770	0.0	27.98175	5
AACGCAG	7625	0.0	26.131119	6
GTACATG	11270	0.0	26.08081	1
TATCAAC	7865	0.0	25.581745	2
TACATGG	11310	0.0	24.938698	2
TCAACGC	7900	0.0	24.27718	4
CGCAGAG	7895	0.0	23.510235	8
ACGCAGA	8030	0.0	23.408295	7
ACATGGG	11245	0.0	23.20164	3
CGTTAAC	435	0.0	22.703615	1
TAGACCG	170	3.4508757E-6	22.120735	5
CATGGGG	7535	0.0	22.084375	4
GAGTACA	8250	0.0	20.920742	1
GTGATCG	815	0.0	20.756634	8
GCAGAGT	8665	0.0	20.607267	9
CTCGTCG	1110	0.0	20.319996	9
CTGTGCG	1960	0.0	19.898823	9
>>END_MODULE