FastQCFastQC Report
Mon 16 Jan 2017
ERR1146524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265359
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126120.3862362453868013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112890.3457200264963209No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62270.19069878687152012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74950.058.59981
GTATCAA143750.043.115011
ATCAACG171550.035.606233
CAACGCA175400.034.8092165
TATCAAC182500.033.8053322
AACGCAG187350.033.114766
TCAACGC185550.033.071754
ACGCAGA203400.029.9684167
CGCAGAG204200.029.804988
GTGGTAT22100.027.4484831
GCAGAGT222050.027.2397149
GTGATCG7900.025.580928
GAGTACT155850.023.55188612-13
CAGAGTA215500.023.4227810-11
GTACATG109650.023.0724961
TGGTATC25500.022.8662932
TACATGG108150.022.7399442
AGAGTAC205400.022.66394210-11
ACATGGG107600.022.244013
TATTCGC2607.1486284E-1021.6911569
Produced by FastQC (version 0.11.5)