##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146524_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3265359
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3556503281875	33.0	31.0	34.0	30.0	34.0
2	32.44664216093851	34.0	31.0	34.0	30.0	34.0
3	32.54081802337814	34.0	31.0	34.0	30.0	34.0
4	36.03224668405526	37.0	35.0	37.0	35.0	37.0
5	36.07921272974886	37.0	35.0	37.0	35.0	37.0
6	36.142907410793114	37.0	36.0	37.0	35.0	37.0
7	36.10545884847577	37.0	36.0	37.0	35.0	37.0
8	36.11524245879243	37.0	36.0	37.0	35.0	37.0
9	37.90574083890929	39.0	38.0	39.0	35.0	39.0
10-11	37.852456804902616	39.0	38.0	39.0	35.0	39.0
12-13	37.82388337698856	39.0	38.0	39.0	35.0	39.0
14-15	39.16314132688014	40.0	39.0	41.0	36.0	41.0
16-17	39.113714755406676	40.0	39.0	41.0	36.0	41.0
18-19	39.22706477296984	40.5	39.0	41.0	36.0	41.0
20-21	39.28567793005302	41.0	39.0	41.0	36.0	41.0
22-23	39.243407692691676	41.0	39.0	41.0	36.0	41.0
24-25	39.2245113018201	41.0	39.0	41.0	36.0	41.0
26-27	39.138104416696606	41.0	39.0	41.0	35.0	41.0
28-29	39.075246397103655	40.0	39.0	41.0	35.0	41.0
30-31	38.91426869143638	40.0	38.5	41.0	35.0	41.0
32-33	38.78012463560668	40.0	38.0	41.0	35.0	41.0
34-35	38.6923627693004	40.0	38.0	41.0	35.0	41.0
36-37	38.608146761198384	40.0	38.0	41.0	35.0	41.0
38-39	38.51069177998499	40.0	38.0	41.0	35.0	41.0
40-41	38.3992957589043	40.0	38.0	41.0	34.0	41.0
42-43	38.27650053179451	40.0	38.0	41.0	34.0	41.0
44-45	38.09635081471899	40.0	38.0	41.0	33.5	41.0
46-47	37.81441872088184	40.0	37.5	41.0	33.0	41.0
48-49	37.49109393484759	40.0	37.0	41.0	32.5	41.0
50-51	37.35884277961473	40.0	37.0	41.0	32.0	41.0
52-53	37.25001156075029	40.0	36.5	41.0	32.0	41.0
54-55	37.090565845899334	40.0	36.0	41.0	31.5	41.0
56-57	36.925569592807406	39.5	35.5	41.0	31.0	41.0
58-59	36.74212789466641	39.0	35.0	41.0	31.0	41.0
60-61	36.54403329006091	39.0	35.0	41.0	31.0	41.0
62-63	36.314890031999546	39.0	35.0	41.0	31.0	41.0
64-65	36.05376652306836	38.0	35.0	40.5	30.5	41.0
66-67	35.79251071627959	37.0	35.0	40.0	30.0	41.0
68-69	35.50836171459248	37.0	35.0	40.0	30.0	41.0
70-71	35.2126265442789	36.0	35.0	39.0	30.0	41.0
72-73	34.89760161133891	36.0	35.0	39.0	29.0	41.0
74-75	34.58868412324648	35.0	34.5	39.0	29.0	40.5
76-77	34.30100687244496	35.0	34.0	37.5	29.0	39.5
78-79	34.01624323696108	35.0	34.0	37.0	29.0	39.0
80-81	33.759476063734496	35.0	34.0	36.5	29.0	39.0
82-83	33.504271046460744	35.0	34.0	36.0	29.0	38.0
84-85	33.29164894273494	35.0	34.0	36.0	29.0	37.0
86-87	33.104489736044336	35.0	34.0	35.5	29.0	37.0
88-89	32.91545921903227	35.0	34.0	35.0	28.0	36.0
90-91	32.82942396226571	35.0	34.0	35.0	28.5	36.0
92-93	32.73007393674018	35.0	34.0	35.0	27.5	36.0
94-95	32.657570270221434	35.0	34.0	35.0	27.0	36.0
96-97	32.59610382809363	35.0	34.0	35.0	27.0	35.5
98-99	32.52986409763827	35.0	34.0	35.0	27.0	35.0
100	31.803708566194405	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2955260189499853
1101	2	0.2925528213249038
1101	3	0.32662769164868166
1101	4	0.2619659554437419
1101	5	0.18877718039426838
1101	6	0.2167031618026627
1101	7	0.23967520783094898
1101	8	0.22295322106388227
1101	9	0.3019358050974148
1101	10-11	0.28283513285084894
1101	12-13	0.3396725650979633
1101	14-15	0.4401709112891865
1101	16-17	0.46300152999396715
1101	18-19	0.42097392351833207
1101	20-21	0.4306905166346766
1101	22-23	0.41130743597858554
1101	24-25	0.40179827203603935
1101	26-27	0.38798637732874397
1101	28-29	0.41440919989994995
1101	30-31	0.43699411137306043
1101	32-33	0.4076047821251123
1101	34-35	0.3906850073938699
1101	36-37	0.45033215098084867
1101	38-39	0.4134612692784998
1101	40-41	0.4129771116251604
1101	42-43	0.46695897867483893
1101	44-45	0.393817886736997
1101	46-47	0.32210192889219513
1101	48-49	0.3339508183529176
1101	50-51	0.3071427602734076
1101	52-53	0.27061944373167535
1101	54-55	0.3110228437570228
1101	56-57	0.3243080210043914
1101	58-59	0.3163890334353141
1101	60-61	0.25055336201457123
1101	62-63	0.2824290712051436
1101	64-65	0.2749790121556046
1101	66-67	0.22254388725965413
1101	68-69	0.23923888496911871
1101	70-71	0.18796499631220343
1101	72-73	0.18089372518149105
1101	74-75	0.2621983964728898
1101	76-77	0.2161863879863688
1101	78-79	0.20250715189756363
1101	80-81	0.23635837821283445
1101	82-83	0.20913557100615776
1101	84-85	0.169231160480237
1101	86-87	0.18174289415706824
1101	88-89	0.2612175620319519
1101	90-91	0.21983970822530097
1101	92-93	0.20394458440122776
1101	94-95	0.27311289085185564
1101	96-97	0.23801222265855415
1101	98-99	0.23969530133119576
1101	100	0.3249450885242382
1102	1	0.33969120420490384
1102	2	0.3228469553431097
1102	3	0.34902006132922736
1102	4	0.2231529674930357
1102	5	0.2492113263070479
1102	6	0.22874746914814637
1102	7	0.17882936830477547
1102	8	0.1853135208398271
1102	9	0.3138372910053988
1102	10-11	0.2624075798064496
1102	12-13	0.3072734378475488
1102	14-15	0.43623223145698375
1102	16-17	0.5201439603555897
1102	18-19	0.47068948885500106
1102	20-21	0.4153844285342885
1102	22-23	0.39540631866532294
1102	24-25	0.4282824218018675
1102	26-27	0.434455610188877
1102	28-29	0.39399652408862096
1102	30-31	0.45666593103238284
1102	32-33	0.4878865695426171
1102	34-35	0.43358313327124165
1102	36-37	0.46078693867882947
1102	38-39	0.42327321023265085
1102	40-41	0.4213793520943838
1102	42-43	0.43907077876713885
1102	44-45	0.4074882005197793
1102	46-47	0.5222272522992029
1102	48-49	0.5559904864060883
1102	50-51	0.5461881877092338
1102	52-53	0.4801126752818874
1102	54-55	0.5052780618187924
1102	56-57	0.4937411317021798
1102	58-59	0.41728865220546396
1102	60-61	0.36341109034343333
1102	62-63	0.38394319483873574
1102	64-65	0.4187262643757421
1102	66-67	0.38009910523727086
1102	68-69	0.381188907324038
1102	70-71	0.33967069663074767
1102	72-73	0.31503169742243387
1102	74-75	0.37195429684364
1102	76-77	0.33694963752992635
1102	78-79	0.3357411594129971
1102	80-81	0.3599162934478315
1102	82-83	0.3279940538361501
1102	84-85	0.38052050986006236
1102	86-87	0.4294722376749718
1102	88-89	0.41522301208838996
1102	90-91	0.38159562566729477
1102	92-93	0.34863971172569563
1102	94-95	0.39774770737619747
1102	96-97	0.38138700306158313
1102	98-99	0.4018800738008963
1102	100	0.3977847429684971
1103	1	0.23113300510836865
1103	2	0.2766081358579626
1103	3	0.27859821684539554
1103	4	0.22466305791014207
1103	5	0.18354730632002259
1103	6	0.2051254805995626
1103	7	0.16763414095947127
1103	8	0.1646046061668045
1103	9	0.2226319885439807
1103	10-11	0.23554766550770978
1103	12-13	0.307475097103044
1103	14-15	0.47448530519129406
1103	16-17	0.48072487240469997
1103	18-19	0.3922707171246458
1103	20-21	0.3767056732976144
1103	22-23	0.37002805062800803
1103	24-25	0.3277358624461755
1103	26-27	0.3963783017200555
1103	28-29	0.4125611417560151
1103	30-31	0.43533261810038226
1103	32-33	0.4354365272883385
1103	34-35	0.41868898868705173
1103	36-37	0.3410894197154093
1103	38-39	0.3511525630712242
1103	40-41	0.3419479684215645
1103	42-43	0.3754600344875598
1103	44-45	0.3768440365150951
1103	46-47	0.36432942841442184
1103	48-49	0.5443213205770476
1103	50-51	0.49957449386879205
1103	52-53	0.46020470879969366
1103	54-55	0.4737205461666605
1103	56-57	0.4509689682122371
1103	58-59	0.44820882260361117
1103	60-61	0.4784212086161972
1103	62-63	0.48859493244317065
1103	64-65	0.47942530794590965
1103	66-67	0.40839240881604866
1103	68-69	0.4169351681926514
1103	70-71	0.38771542575214824
1103	72-73	0.32392490982298483
1103	74-75	0.32693151405576515
1103	76-77	0.28742012348165247
1103	78-79	0.3347736657864502
1103	80-81	0.3525957486068947
1103	82-83	0.3035762174281018
1103	84-85	0.36897885231514493
1103	86-87	0.32177305208018936
1103	88-89	0.37216797637429977
1103	90-91	0.26269866256152596
1103	92-93	0.2690902357651339
1103	94-95	0.26378534625233385
1103	96-97	0.2993295372357636
1103	98-99	0.29435665347159556
1103	100	0.3094297410735223
1104	1	0.28273145896929464
1104	2	0.22007473395019872
1104	3	0.22653179608234097
1104	4	0.18633354170809469
1104	5	0.13828962986810467
1104	6	0.184473862805568
1104	7	0.15233652526021046
1104	8	0.1697098652361717
1104	9	0.21839783901422294
1104	10-11	0.2626839062896664
1104	12-13	0.27705416797311244
1104	14-15	0.45284814427979114
1104	16-17	0.5098435838483155
1104	18-19	0.45752981328153197
1104	20-21	0.3983377714876326
1104	22-23	0.4047677490612358
1104	24-25	0.34822884830001755
1104	26-27	0.34824987467280266
1104	28-29	0.3564895993403141
1104	30-31	0.46769709654630987
1104	32-33	0.4560853056723886
1104	34-35	0.37826575588490385
1104	36-37	0.33656010548647686
1104	38-39	0.37897853938625303
1104	40-41	0.3637148582229912
1104	42-43	0.3494119573841843
1104	44-45	0.3455369971892708
1104	46-47	0.4463485971102017
1104	48-49	0.5386628587336304
1104	50-51	0.5042389747255385
1104	52-53	0.4404253255945392
1104	54-55	0.41310045653774097
1104	56-57	0.3899475306672002
1104	58-59	0.41962598281865837
1104	60-61	0.3654301313279973
1104	62-63	0.37354761340866816
1104	64-65	0.3851366738896047
1104	66-67	0.45441517231196116
1104	68-69	0.411289751562812
1104	70-71	0.4113986493931776
1104	72-73	0.32071032275623423
1104	74-75	0.3470368133054649
1104	76-77	0.3751425684814791
1104	78-79	0.37936558096845374
1104	80-81	0.31742006681367485
1104	82-83	0.28699125076438037
1104	84-85	0.2445783294351216
1104	86-87	0.28991326535737727
1104	88-89	0.4017516813756785
1104	90-91	0.3359584658922117
1104	92-93	0.34603650395007435
1104	94-95	0.37544222231209545
1104	96-97	0.37012462559231096
1104	98-99	0.3064258600708172
1104	100	0.41537144745174714
1105	1	0.20419318093849625
1105	2	0.19297432189208052
1105	3	0.18646239681970656
1105	4	0.13059292145218393
1105	5	0.14678670208671463
1105	6	0.14734525729700465
1105	7	0.1363703574007431
1105	8	0.10594061778442665
1105	9	0.2200113718704344
1105	10-11	0.19396562392141448
1105	12-13	0.21436711527304197
1105	14-15	0.35865165761746454
1105	16-17	0.35958767406736314
1105	18-19	0.3043331097465085
1105	20-21	0.27292246027531064
1105	22-23	0.23531209952751908
1105	24-25	0.2004214312538224
1105	26-27	0.20343963828156575
1105	28-29	0.23876676264546148
1105	30-31	0.2971419024452473
1105	32-33	0.2538905539916243
1105	34-35	0.25474374330628535
1105	36-37	0.2913876916933802
1105	38-39	0.33256127631968013
1105	40-41	0.2709258753559425
1105	42-43	0.2871196129903453
1105	44-45	0.2784908033050755
1105	46-47	0.2973678987801236
1105	48-49	0.38591941913267647
1105	50-51	0.31254736769886904
1105	52-53	0.3043030093941468
1105	54-55	0.27209633595651894
1105	56-57	0.2504376629595484
1105	58-59	0.15518876746407528
1105	60-61	0.20530651389142918
1105	62-63	0.2424393939335019
1105	64-65	0.2386716988299611
1105	66-67	0.1783058684733092
1105	68-69	0.19408957806466276
1105	70-71	0.203190017859896
1105	72-73	0.21975174544111553
1105	74-75	0.13711488747593137
1105	76-77	0.15106102434788937
1105	78-79	0.20221095073640782
1105	80-81	0.22740488572497242
1105	82-83	0.2409817083657657
1105	84-85	0.16604655758519726
1105	86-87	0.1283214197707352
1105	88-89	0.23773086159688717
1105	90-91	0.1992657745020594
1105	92-93	0.1877085924341344
1105	94-95	0.19120236111061928
1105	96-97	0.19134995632316532
1105	98-99	0.18321614417405385
1105	100	0.192053295207117
1106	1	0.08339914965590367
1106	2	0.10767236831571125
1106	3	0.12136880624091617
1106	4	0.09103542575638812
1106	5	0.07879214168514892
1106	6	0.07068446957449481
1106	7	0.08632092750711706
1106	8	0.11955277464009129
1106	9	0.12454062301279833
1106	10-11	0.1395760799575143
1106	12-13	0.11997358947604653
1106	14-15	0.21085501560204278
1106	16-17	0.30045087219897937
1106	18-19	0.15415002802890143
1106	20-21	0.14466228192447517
1106	22-23	0.14158067248977346
1106	24-25	0.06481040258420734
1106	26-27	0.12581671423559015
1106	28-29	0.15486111080860354
1106	30-31	0.2082921411802232
1106	32-33	0.15836219176130584
1106	34-35	0.18244064068265686
1106	36-37	0.1352172294745131
1106	38-39	0.1278822444091503
1106	40-41	0.13720572737847192
1106	42-43	0.19034314769606198
1106	44-45	0.22381627265805548
1106	46-47	0.27777599186988766
1106	48-49	0.3090530993783176
1106	50-51	0.265220327173914
1106	52-53	0.22699300366640784
1106	54-55	0.22950696277461446
1106	56-57	0.25547634792417284
1106	58-59	0.29005362245268174
1106	60-61	0.24425525395066927
1106	62-63	0.2880581244539897
1106	64-65	0.2159132965530901
1106	66-67	0.2629782051918994
1106	68-69	0.19813903511987263
1106	70-71	0.2041336731014738
1106	72-73	0.23367857071746556
1106	74-75	0.2958072473266711
1106	76-77	0.25446092737821147
1106	78-79	0.2088040766979944
1106	80-81	0.22200826980706978
1106	82-83	0.221265124156929
1106	84-85	0.2101205776783388
1106	86-87	0.20300291162526918
1106	88-89	0.24241714593197372
1106	90-91	0.2328621412333618
1106	92-93	0.25752815967160103
1106	94-95	0.16100658344979735
1106	96-97	0.16955481043532927
1106	98-99	0.22040106097907852
1106	100	0.27151326679385335
1107	1	0.12559756593266513
1107	2	0.06158244681659397
1107	3	0.08228918697409426
1107	4	0.10869572203326783
1107	5	0.10063283063503548
1107	6	0.10695253501116753
1107	7	0.1219524797092717
1107	8	0.12167841339680052
1107	9	0.1377638636682832
1107	10-11	0.16691034403962135
1107	12-13	0.16390961346014166
1107	14-15	0.26560308525917264
1107	16-17	0.2708614594934815
1107	18-19	0.23890844578498616
1107	20-21	0.2125992149024043
1107	22-23	0.19641805070064322
1107	24-25	0.18862833895065023
1107	26-27	0.21519440788217992
1107	28-29	0.22283835840644883
1107	30-31	0.2767518951790109
1107	32-33	0.2528984344912999
1107	34-35	0.15468714081164592
1107	36-37	0.134092181908251
1107	38-39	0.16219039386339773
1107	40-41	0.16253250921215567
1107	42-43	0.16556157634050095
1107	44-45	0.17540389819537694
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2216	44-45	-0.5866499606289892
2216	46-47	-0.5576493154901101
2216	48-49	-0.4981451818215632
2216	50-51	-0.4807763419085944
2216	52-53	-0.5212796665438262
2216	54-55	-0.4952670342451668
2216	56-57	-0.5069580348283509
2216	58-59	-0.5242776240453608
2216	60-61	-0.5757113645514238
2216	62-63	-0.5420052425336408
2216	64-65	-0.46438891708849184
2216	66-67	-0.48413347494460623
2216	68-69	-0.4814536202654267
2216	70-71	-0.5191756566141947
2216	72-73	-0.45450377125808217
2216	74-75	-0.42806298202093274
2216	76-77	-0.4310701904103027
2216	78-79	-0.38479142808598255
2216	80-81	-0.408608180854948
2216	82-83	-0.4046067960259023
2216	84-85	-0.3854700263987496
2216	86-87	-0.31146929807788837
2216	88-89	-0.2926990885223901
2216	90-91	-0.34640625976383177
2216	92-93	-0.3484828858243816
2216	94-95	-0.30176876093449323
2216	96-97	-0.30007340628797863
2216	98-99	-0.3935178208010086
2216	100	-0.5289433703751847
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	29.0
8	121.0
9	292.0
10	340.0
11	227.0
12	211.0
13	312.0
14	550.0
15	1163.0
16	2154.0
17	3744.0
18	6206.0
19	9163.0
20	12929.0
21	19343.0
22	26800.0
23	28890.0
24	26062.0
25	21413.0
26	19550.0
27	21818.0
28	27309.0
29	32590.0
30	39918.0
31	50025.0
32	63694.0
33	83445.0
34	120297.0
35	186946.0
36	332884.0
37	744712.0
38	1010255.0
39	371138.0
40	829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.91476329603155	20.612876156875714	13.662526108181277	23.80983443891145
2	15.211352753171884	21.29195541416099	40.215915953806935	23.28077587886019
3	17.3144073376514	27.15004517126801	31.70609582433736	23.82945166674323
4	11.91364964042642	18.47761776643696	39.904930773928086	29.70380181920853
5	12.095213040771245	38.72262207041568	35.778268668381486	13.40389622043159
6	28.218709631184062	39.9696680699472	18.27786669508754	13.5337556037812
7	24.469223751507872	34.45970259319113	23.777293706450042	17.293779948850954
8	25.033587609992342	35.09207767274536	21.129615559293807	18.744719157968497
9	26.176984629858236	14.663327777024074	21.66145998737657	37.49822760574112
10-11	23.909579245022712	26.549030710735565	29.17730422390327	20.364085820338452
12-13	25.016116145269173	24.903831401080247	28.909593095276815	21.170459358373765
14-15	22.35260106401875	25.598081801800475	27.49109132358003	24.558225810600742
16-17	20.842302517925354	28.39473109596009	28.31247337572393	22.450493010390627
18-19	20.54006064577042	27.259007480196264	31.242616615556866	20.95831525847645
20-21	21.89088123924216	26.37505529651411	30.891645475730705	20.842417988513027
22-23	21.850583657110903	26.279009444290814	30.52786845183026	21.342538446768028
24-25	21.478189687565745	26.514374070354897	30.896158737829438	21.111277504249916
26-27	21.382151143276438	26.632350815471046	30.569721120418258	21.41577692083426
28-29	21.018136293457516	26.904274063628847	30.723012496177677	21.354577146735956
30-31	21.54656456031483	26.55936026528215	30.617312949424925	21.27676222497809
32-33	21.55132905415762	26.810737046903267	30.206714535982915	21.431219362956206
34-35	21.750454850725358	26.691706297351253	30.449947739251787	21.107891112671602
36-37	21.811411010441436	26.377407663179042	30.35152380062811	21.45965752575141
38-39	21.58790003403942	26.42572431007388	30.573052077560884	21.413323578325816
40-41	21.686820782412113	26.124814643997034	30.637594025488323	21.550770548102523
42-43	21.716524890525054	26.334271361893137	30.143576862452182	21.805626885129627
44-45	21.750297786617416	26.777428945793265	29.89581641001408	21.57645685757523
46-47	21.944432449847014	26.668277515580986	29.19700406601541	22.190285968556598
48-49	22.28684336812177	26.634867182975707	29.08678897896922	21.991500469933303
50-51	22.117690581648144	26.919949690064705	28.56762457052961	22.394735157757538
52-53	21.819305742472196	27.227555928631723	28.56124369113274	22.391894637763336
54-55	21.874741604718153	27.098858742687604	28.489538321513248	22.536861331080992
56-57	22.33050382898761	26.913575067205574	28.19208882099766	22.563832282809155
58-59	21.80804613559144	26.997105806868305	28.71374621535363	22.48110184218662
60-61	21.912547278745574	27.19687971380635	28.482151101194162	22.40842190625391
62-63	21.874676049622195	26.819822111143555	28.308007081009762	22.997494758224494
64-65	21.426847699094584	26.939343315943642	28.520569801965795	23.11323918299598
66-67	21.906337486948015	26.716143359808115	28.37387340277343	23.00364575047044
68-69	21.633324191696303	26.63229901146288	28.520024089821405	23.214352707019415
70-71	21.68598190432017	26.49044660990437	28.46896007646571	23.35461140930975
72-73	21.852816666110307	26.41124247035689	28.54528321013236	23.19065765340045
74-75	21.78594588966158	26.756863324577047	28.341754018998415	23.115436766762958
76-77	21.70028907584849	26.6153594552058	28.125435458970312	23.5589160099754
78-79	21.559608724293096	26.7354889369155	28.033616317425647	23.671286021365752
80-81	21.456238726254316	26.697225089041538	28.302591162302125	23.543945022402024
82-83	21.736379534776606	26.31911064547905	28.596319917607214	23.348189902137133
84-85	21.85984314930063	26.498341900347423	27.89213478741611	23.749680162935835
86-87	21.221066351356775	26.726555946834633	28.15704184440363	23.89533585740496
88-89	21.54842698766047	26.246624031232095	28.006614280389996	24.198334700717442
90-91	21.413903378114036	26.160719002436945	28.15217016819751	24.273207451251512
92-93	21.54496548537073	26.20444426478044	28.08264768802285	24.167942561825985
94-95	20.97370408826849	26.08732801068674	28.320254869645893	24.61871303139888
96-97	21.137277330019746	25.95788956288659	28.144520827447657	24.76031227964601
98-99	20.826957685605038	26.259905318090272	28.00424884955064	24.90888814675405
100	21.157312217108593	25.90015743388589	27.786789712521493	25.155740636484026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	284.0
1	184.5
2	268.5
3	440.0
4	284.5
5	202.0
6	450.5
7	1160.0
8	2170.5
9	2704.0
10	2104.5
11	1450.5
12	1472.5
13	1645.0
14	2034.5
15	2486.5
16	2899.0
17	3373.0
18	4016.5
19	4692.5
20	5474.5
21	6478.5
22	7775.5
23	9416.0
24	11291.0
25	13976.0
26	18900.0
27	24386.5
28	28882.5
29	35003.5
30	43167.0
31	51119.5
32	60162.5
33	67449.5
34	74774.5
35	82531.0
36	89075.0
37	99108.5
38	102709.0
39	99400.5
40	99231.5
41	100472.5
42	102091.5
43	106438.0
44	110670.0
45	118459.0
46	125882.0
47	130307.0
48	133480.0
49	130737.5
50	126812.0
51	123682.0
52	119415.0
53	115523.0
54	110799.5
55	98872.0
56	87253.5
57	74796.5
58	62107.0
59	55243.0
60	44391.5
61	32574.5
62	24824.5
63	18960.0
64	13459.0
65	8305.0
66	5453.5
67	4018.5
68	2933.0
69	2351.0
70	1931.5
71	1489.0
72	1322.5
73	1324.0
74	1130.5
75	880.5
76	672.5
77	510.5
78	383.0
79	274.0
80	212.0
81	140.0
82	98.0
83	69.5
84	39.5
85	25.5
86	21.5
87	13.0
88	6.0
89	4.0
90	3.5
91	2.0
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0030318259033692775
2	0.0032768219359647745
3	2.756205366699343E-4
4	0.04997919064948142
5	0.002817454374848217
6	0.004685549123388883
7	0.0
8	5.512410733398686E-4
9	6.124900814887428E-5
10-11	7.656126018609286E-5
12-13	0.0
14-15	9.187351222331143E-5
16-17	3.6749404889324574E-4
18-19	1.0718576426053001E-4
20-21	2.6030828463271573E-4
22-23	0.0
24-25	0.0
26-27	2.1437152852106002E-4
28-29	1.531225203721857E-5
30-31	1.6843477240940428E-4
32-33	3.2155729278159E-4
34-35	6.124900814887428E-5
36-37	1.2249801629774857E-4
38-39	8.421738620470214E-4
40-41	6.4311458556318E-4
42-43	0.0
44-45	5.3592882130265E-4
46-47	0.0
48-49	6.124900814887428E-5
50-51	0.0
52-53	4.440553090793386E-4
54-55	3.981185529676829E-4
56-57	1.5312252037218572E-4
58-59	8.421738620470214E-4
60-61	5.3592882130265E-4
62-63	1.6843477240940428E-4
64-65	4.2874305704212004E-4
66-67	0.0015465374557590758
68-69	0.002434648073917753
70-71	0.003200260675778682
72-73	0.00300120139929484
74-75	0.002649019602438813
76-77	0.0038586875133790805
78-79	0.0012249801629774858
80-81	8.881106181586772E-4
82-83	7.5030034982371E-4
84-85	7.5030034982371E-4
86-87	0.0
88-89	0.0
90-91	4.593675611165572E-5
92-93	1.3781026833496716E-4
94-95	1.0718576426053001E-4
96-97	5.053043172282129E-4
98-99	1.3781026833496716E-4
100	0.07356005878679801
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3265359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.228662847957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.71902115012199	31.695405137469884
2	16.004911814865	15.43790991660815
3	7.027271660420123	10.16747746954215
4	3.732824846124789	7.201166038969175
5	2.1676576760243584	5.22716156133824
6	1.403472640255958	4.061256526994198
7	0.9339280341758951	3.152947019916715
8	0.6541402687132566	2.523864838003491
9	0.48188962311694167	2.091680294691244
>10	1.827403279079754	13.659334195874415
>50	0.031211090644911288	0.9943315649724447
>100	0.013705744499545237	1.216938153007845
>500	0.001559582930632474	0.5079806762900364
>1k	6.68392684556774E-4	0.4438853884197015
>5k	1.1139878075946232E-4	0.3345526886576472
>10k+	2.2279756151892465E-4	1.2841085292446484
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12612	0.3862362453868013	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11289	0.3457200264963209	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6227	0.19069878687152012	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	1.531225203721857E-5	0.0
16-17	0.0	0.0	0.0	4.593675611165571E-5	0.0
18-19	0.0	0.0	0.0	9.187351222331143E-5	0.0
20-21	0.0	0.0	0.0	1.3781026833496716E-4	0.0
22-23	0.0	0.0	0.0	4.5936756111655716E-4	0.0
24-25	0.0	0.0	0.0	0.0011943556589030487	0.0
26-27	0.0	0.0	0.0	0.0024193358218805344	0.0
28-29	0.0	0.0	0.0	0.007747999530832598	0.0
30-31	0.0	0.0	0.0	0.02361149264139104	0.0
32-33	0.0	0.0	0.0	0.056073466960294416	0.0
34-35	0.0	0.0	0.0	0.10847199343165637	0.0
36-37	0.0	0.0	0.0	0.19008629679003136	0.0
38-39	0.0	0.0	0.0	0.31255368858370547	0.0
40-41	0.0	0.0	0.0	0.448848043966988	0.0
42-43	0.0	0.0	0.0	0.5890163991156868	0.0
44-45	1.531225203721857E-5	0.0	0.0	0.7586302149319569	0.0
46-47	3.062450407443714E-5	0.0	0.0	0.9632937756614204	0.0
48-49	3.062450407443714E-5	0.0	0.0	1.1922578803739499	0.0
50-51	6.124900814887428E-5	0.0	0.0	1.4376826560264888	0.0
52-53	9.187351222331143E-5	0.0	0.0	1.6783759457995278	0.0
54-55	9.187351222331143E-5	0.0	0.0	1.9410882540020866	0.0
56-57	9.187351222331143E-5	1.531225203721857E-5	0.0	2.249722006064264	0.0
58-59	9.187351222331143E-5	3.062450407443714E-5	0.0	2.5963454554307814	0.0
60-61	1.2249801629774857E-4	3.062450407443714E-5	0.0	2.965906658349051	0.0
62-63	1.5312252037218572E-4	3.062450407443714E-5	0.0	3.3427258687329635	0.0
64-65	1.5312252037218572E-4	3.062450407443714E-5	0.0	3.7347807698939075	0.0
66-67	1.684347724094043E-4	3.062450407443714E-5	0.0	4.1753448855087605	0.0
68-69	1.8374702444662287E-4	3.062450407443714E-5	0.0	4.668797519660166	0.0
70-71	1.8374702444662287E-4	3.062450407443714E-5	0.0	5.217236450877223	0.0
72-73	2.1437152852106002E-4	3.062450407443714E-5	0.0	5.769227824566916	0.0
74-75	2.1437152852106002E-4	3.062450407443714E-5	0.0	6.318462992889909	0.0
76-77	2.1437152852106002E-4	3.062450407443714E-5	0.0	6.9137267908367805	0.0
78-79	2.1437152852106002E-4	3.062450407443714E-5	0.0	7.561664735791685	0.0
80-81	2.1437152852106002E-4	3.062450407443714E-5	0.0	8.284663340233035	0.0
82-83	2.1437152852106002E-4	3.062450407443714E-5	0.0	9.022422955638262	0.0
84-85	2.1437152852106002E-4	3.062450407443714E-5	0.0	9.748484010487054	0.0
86-87	2.1437152852106002E-4	3.062450407443714E-5	0.0	10.492383838959206	0.0
88	2.1437152852106002E-4	3.062450407443714E-5	0.0	11.08187491788805	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7495	0.0	58.5998	1
GTATCAA	14375	0.0	43.11501	1
ATCAACG	17155	0.0	35.60623	3
CAACGCA	17540	0.0	34.809216	5
TATCAAC	18250	0.0	33.805332	2
AACGCAG	18735	0.0	33.11476	6
TCAACGC	18555	0.0	33.07175	4
ACGCAGA	20340	0.0	29.968416	7
CGCAGAG	20420	0.0	29.80498	8
GTGGTAT	2210	0.0	27.448483	1
GCAGAGT	22205	0.0	27.239714	9
GTGATCG	790	0.0	25.58092	8
GAGTACT	15585	0.0	23.551886	12-13
CAGAGTA	21550	0.0	23.42278	10-11
GTACATG	10965	0.0	23.072496	1
TGGTATC	2550	0.0	22.866293	2
TACATGG	10815	0.0	22.739944	2
AGAGTAC	20540	0.0	22.663942	10-11
ACATGGG	10760	0.0	22.24401	3
TATTCGC	260	7.1486284E-10	21.691156	9
>>END_MODULE