FastQCFastQC Report
Mon 16 Jan 2017
ERR1146523_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146523_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466941
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT101890.29389020465015125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG119750.026.1564181
GTACTAG11800.025.5080661
CTAACGC6100.025.4350133
TACATGG119300.025.0254062
ACATGGG122300.023.4888943
TCTAACG6300.022.3886832
ATACGGG9150.021.0673833
CATGGGG89750.020.5873134
GGCGTCG9700.020.3501978
TAACGCC7250.020.1032034
TACGGGC9850.020.0472414
TAGTACT16850.019.8107094
TGATCGC9700.019.379189
CGGGCGT10000.019.2694196
CTAGTAC17150.018.9161533
GTGATCG10050.018.238528
GAGTACA101800.017.925181
GTATAAG17100.017.6020581
GTCCTAC12250.017.276511
CCTAGTA19050.017.2763062
Produced by FastQC (version 0.11.5)