##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146523_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3466941
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20660374664582	31.0	31.0	34.0	28.0	34.0
2	31.234767479458117	31.0	31.0	34.0	28.0	34.0
3	31.441349016323034	33.0	31.0	34.0	28.0	34.0
4	34.90054575488882	37.0	35.0	37.0	32.0	37.0
5	34.92831605729662	37.0	35.0	37.0	32.0	37.0
6	34.93770156457811	37.0	35.0	37.0	32.0	37.0
7	34.926965875681184	37.0	35.0	37.0	32.0	37.0
8	34.86675775561223	37.0	35.0	37.0	32.0	37.0
9	36.45919673856579	39.0	37.0	39.0	32.0	39.0
10-11	36.45179208414565	39.0	37.0	39.0	32.0	39.0
12-13	36.40146154780251	39.0	37.0	39.0	32.0	39.0
14-15	37.73550992070531	40.0	38.0	41.0	32.0	41.0
16-17	37.765862182252306	40.0	38.0	41.0	32.0	41.0
18-19	37.792171109920815	40.0	38.0	41.0	32.0	41.0
20-21	37.89613004663188	40.0	38.0	41.0	32.0	41.0
22-23	37.9154796692531	40.0	38.0	41.0	32.0	41.0
24-25	37.93592665695782	40.0	38.0	41.0	32.0	41.0
26-27	37.99947922390372	40.0	38.0	41.0	32.0	41.0
28-29	37.9846687613086	40.0	38.0	41.0	32.0	41.0
30-31	37.94976984032898	40.0	38.0	41.0	32.0	41.0
32-33	37.91188370381844	40.0	38.0	41.0	31.0	41.0
34-35	37.85457410437616	40.0	38.0	41.0	31.0	41.0
36-37	37.77429223629707	40.0	38.0	41.0	31.0	41.0
38-39	37.71102623321251	40.0	38.0	41.0	31.0	41.0
40-41	37.65824180451874	40.0	37.0	41.0	31.0	41.0
42-43	37.497051002598546	40.0	37.0	41.0	30.0	41.0
44-45	37.359793547106804	40.0	37.0	41.0	30.0	41.0
46-47	37.23537204700051	40.0	36.5	41.0	30.0	41.0
48-49	37.11428605217107	40.0	36.0	41.0	30.0	41.0
50-51	36.969552409458366	40.0	36.0	41.0	30.0	41.0
52-53	36.82950618427023	39.0	35.0	41.0	30.0	41.0
54-55	36.69728732043608	39.0	35.0	41.0	30.0	41.0
56-57	36.48093982562726	39.0	35.0	41.0	29.5	41.0
58-59	36.270730883507966	39.0	35.0	40.5	29.0	41.0
60-61	36.02179414071367	38.0	35.0	40.0	29.0	41.0
62-63	35.78247509836481	37.5	35.0	40.0	29.0	41.0
64-65	35.47781675546253	37.0	34.0	40.0	28.0	41.0
66-67	35.20899793218287	36.5	34.0	39.5	28.0	41.0
68-69	34.92515116351851	36.0	34.0	39.0	28.0	41.0
70-71	34.62524138714792	35.5	34.0	39.0	28.0	41.0
72-73	34.28571801481479	35.0	34.0	38.5	27.5	40.0
74-75	33.97075779483989	35.0	33.5	37.0	27.0	39.5
76-77	33.71484819037878	35.0	33.0	37.0	27.0	39.0
78-79	33.417963991887945	35.0	33.0	36.0	27.0	39.0
80-81	33.18079655811853	35.0	33.0	36.0	26.5	38.0
82-83	32.94102091728703	35.0	33.0	35.5	26.0	37.0
84-85	32.75787978509008	35.0	33.0	35.0	26.0	37.0
86-87	32.585761626748194	35.0	33.0	35.0	26.0	36.5
88-89	32.41985730359992	35.0	33.0	35.0	25.5	36.0
90-91	32.27155351071737	35.0	33.0	35.0	25.0	36.0
92-93	32.1225521288075	35.0	33.0	35.0	25.0	36.0
94-95	32.01276283617172	35.0	32.0	35.0	25.0	35.0
96-97	31.927525302564998	35.0	32.0	35.0	25.0	35.0
98-99	31.825366656080966	35.0	32.0	35.0	24.5	35.0
100	30.641509042120994	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29537614928425526
1101	2	0.27374728208124566
1101	3	0.32729644737061037
1101	4	0.3045686239271248
1101	5	0.2889857478890363
1101	6	0.3473059685661255
1101	7	0.3560317658742136
1101	8	0.4289024959065699
1101	9	0.5429828089730719
1101	10-11	0.5224759401785022
1101	12-13	0.5038852136975791
1101	14-15	0.5627395346387871
1101	16-17	0.5459322165304386
1101	18-19	0.5194813448211164
1101	20-21	0.5342708839461707
1101	22-23	0.5341039087766575
1101	24-25	0.6226154325628954
1101	26-27	0.5859575746831922
1101	28-29	0.5867861850297942
1101	30-31	0.6114874620967257
1101	32-33	0.5987236511157832
1101	34-35	0.5618173273102727
1101	36-37	0.6594918170809336
1101	38-39	0.648082856408351
1101	40-41	0.6243996068729771
1101	42-43	0.6630248309582782
1101	44-45	0.5850454825885336
1101	46-47	0.5764255263332814
1101	48-49	0.5738382392156609
1101	50-51	0.5603934162754385
1101	52-53	0.5302094329093165
1101	54-55	0.5543624192466012
1101	56-57	0.5138550055526849
1101	58-59	0.5119269216751761
1101	60-61	0.4720111578911954
1101	62-63	0.501653366714919
1101	64-65	0.5836374748702653
1101	66-67	0.5661635056648961
1101	68-69	0.5709440693927164
1101	70-71	0.3996869963202556
1101	72-73	0.47337421338090735
1101	74-75	0.46868363645928923
1101	76-77	0.471501281654767
1101	78-79	0.47212798544574497
1101	80-81	0.4341591305790189
1101	82-83	0.38520679508808797
1101	84-85	0.36568002890012963
1101	86-87	0.43491529255326355
1101	88-89	0.4199075260333487
1101	90-91	0.36051592398715826
1101	92-93	0.405711647024134
1101	94-95	0.42405239377438875
1101	96-97	0.42566667446812545
1101	98-99	0.3545911916255804
1101	100	0.5473415105053441
1102	1	0.29732720362454756
1102	2	0.3106419978145212
1102	3	0.3063639279021757
1102	4	0.3310057422956092
1102	5	0.27665765621960503
1102	6	0.3018679428625646
1102	7	0.37297598608627425
1102	8	0.41468153214481873
1102	9	0.466735237684901
1102	10-11	0.41055331477217294
1102	12-13	0.4397644543003665
1102	14-15	0.4785561501605784
1102	16-17	0.4504092370449513
1102	18-19	0.4268561204055956
1102	20-21	0.44639285453511945
1102	22-23	0.4421015568471134
1102	24-25	0.5099734936243223
1102	26-27	0.4775025920064948
1102	28-29	0.5234977590237051
1102	30-31	0.5400492142515105
1102	32-33	0.5196125411168779
1102	34-35	0.5006577989012158
1102	36-37	0.5218197691899604
1102	38-39	0.47472942605711665
1102	40-41	0.46473625734839885
1102	42-43	0.506828979790491
1102	44-45	0.4471658747012768
1102	46-47	0.5365122395386877
1102	48-49	0.4770515162795306
1102	50-51	0.5232968913966758
1102	52-53	0.4325970899183744
1102	54-55	0.38228473935253504
1102	56-57	0.49393225367576576
1102	58-59	0.43526629973822395
1102	60-61	0.443829558631073
1102	62-63	0.3695265827360288
1102	64-65	0.449256694242564
1102	66-67	0.40667128606074954
1102	68-69	0.3919821879568701
1102	70-71	0.37372693388574874
1102	72-73	0.41357657788050517
1102	74-75	0.38011035565328655
1102	76-77	0.4127088900640814
1102	78-79	0.33081189529666943
1102	80-81	0.31798819046072424
1102	82-83	0.27145987468072974
1102	84-85	0.2404147799296794
1102	86-87	0.2639345814362528
1102	88-89	0.2711379963605438
1102	90-91	0.3047289655308276
1102	92-93	0.2942548886579459
1102	94-95	0.25412026247634856
1102	96-97	0.32013442335989595
1102	98-99	0.4041250984013587
1102	100	0.47668622054552046
1103	1	0.2054348931255845
1103	2	0.29043077663209615
1103	3	0.29815784776720733
1103	4	0.26694543753569633
1103	5	0.27132558138409735
1103	6	0.3069324074776958
1103	7	0.3183606122228966
1103	8	0.37929523728929126
1103	9	0.46402421406630623
1103	10-11	0.4721729202334757
1103	12-13	0.5021173416205897
1103	14-15	0.5579543519519845
1103	16-17	0.5519577258591752
1103	18-19	0.5178243026088936
1103	20-21	0.4585111056833284
1103	22-23	0.5080122216255489
1103	24-25	0.5008235853541052
1103	26-27	0.5130220812997948
1103	28-29	0.4326734693341052
1103	30-31	0.5665591594588122
1103	32-33	0.5624904293881698
1103	34-35	0.5560553310337824
1103	36-37	0.5104562186700079
1103	38-39	0.5226865481688492
1103	40-41	0.45908884937458083
1103	42-43	0.47854917023524024
1103	44-45	0.4675299449260635
1103	46-47	0.43858709630547565
1103	48-49	0.43870058781546106
1103	50-51	0.45897856623496835
1103	52-53	0.4510576942425075
1103	54-55	0.36067005726791024
1103	56-57	0.469295285983641
1103	58-59	0.40161171351379465
1103	60-61	0.35279266447295043
1103	62-63	0.40398886238337184
1103	64-65	0.39577931972318936
1103	66-67	0.39318218844051245
1103	68-69	0.37386276856972245
1103	70-71	0.2786141858911151
1103	72-73	0.32446925021418593
1103	74-75	0.33665321067526577
1103	76-77	0.37857413438574383
1103	78-79	0.3618871595855069
1103	80-81	0.3553781467270838
1103	82-83	0.34208370911631647
1103	84-85	0.27599836720849424
1103	86-87	0.3512124476509584
1103	88-89	0.24305778621324237
1103	90-91	0.3405892100286749
1103	92-93	0.23951715413768682
1103	94-95	0.30758847690957936
1103	96-97	0.3081461323474386
1103	98-99	0.3176433002162824
1103	100	0.2868726796402612
1104	1	0.1491130690125324
1104	2	0.1390816614656778
1104	3	0.18457308590095778
1104	4	0.16854673997426772
1104	5	0.19920687504676238
1104	6	0.19540575075830446
1104	7	0.25141201267932445
1104	8	0.335942542541396
1104	9	0.3412938459726931
1104	10-11	0.35678633117351666
1104	12-13	0.3820821754708348
1104	14-15	0.4426817022795362
1104	16-17	0.3694712463095442
1104	18-19	0.342135763891811
1104	20-21	0.32377664485265
1104	22-23	0.3202219278392704
1104	24-25	0.3619538957755353
1104	26-27	0.32468070210376965
1104	28-29	0.3691536315015469
1104	30-31	0.35283771744534675
1104	32-33	0.3990086113301814
1104	34-35	0.30615170833523564
1104	36-37	0.2971585155252896
1104	38-39	0.3715253044887348
1104	40-41	0.3785134203435305
1104	42-43	0.32119287928613005
1104	44-45	0.2738307587874047
1104	46-47	0.31194597290765813
1104	48-49	0.2641396849426201
1104	50-51	0.29632955514747295
1104	52-53	0.2948346290211106
1104	54-55	0.2564502135327871
1104	56-57	0.30719783148379065
1104	58-59	0.306931787672049
1104	60-61	0.37083419546537044
1104	62-63	0.31928625746339634
1104	64-65	0.3696642621876549
1104	66-67	0.3118471777708933
1104	68-69	0.2855288811026
1104	70-71	0.27677682759894395
1104	72-73	0.25274327067170077
1104	74-75	0.3043693847482629
1104	76-77	0.21267650147223094
1104	78-79	0.1992526050932284
1104	80-81	0.22845410240654473
1104	82-83	0.1980314265291554
1104	84-85	0.23907365295862348
1104	86-87	0.2422272389538307
1104	88-89	0.21878732597607353
1104	90-91	0.2860247938819427
1104	92-93	0.31508935902674295
1104	94-95	0.22830885140521673
1104	96-97	0.25480615768996273
1104	98-99	0.26367483846740924
1104	100	0.3478065645544568
1105	1	0.2190942062161163
1105	2	0.1571997775911136
1105	3	0.17217348875146143
1105	4	0.17989728450903186
1105	5	-0.001371765507471423
1105	6	0.0683465147523421
1105	7	0.10182004303855763
1105	8	0.14229036308197607
1105	9	0.22240070170337845
1105	10-11	0.26355337462214123
1105	12-13	0.28110805808760375
1105	14-15	0.2666199437858907
1105	16-17	0.27204012923628795
1105	18-19	0.27629875421585126
1105	20-21	0.2779875214100116
1105	22-23	0.22384540887226478
1105	24-25	0.3065874444147809
1105	26-27	0.2624301832042093
1105	28-29	0.24186221612555414
1105	30-31	0.3004338585957811
1105	32-33	0.3039934597473106
1105	34-35	0.2635066957634251
1105	36-37	0.2582142958572007
1105	38-39	0.3045508393442802
1105	40-41	0.2702523646038202
1105	42-43	0.25815542873343134
1105	44-45	0.219237243315483
1105	46-47	0.3010698421250666
1105	48-49	0.24612234238277608
1105	50-51	0.25447228675502487
1105	52-53	0.2169085697205162
1105	54-55	0.17466457339008912
1105	56-57	0.27708646257616465
1105	58-59	0.2333284694774349
1105	60-61	0.22002238019646114
1105	62-63	0.2929146827184965
1105	64-65	0.31900412525261146
1105	66-67	0.31427169509984765
1105	68-69	0.28418002662239417
1105	70-71	0.2900637643303696
1105	72-73	0.3034258739569964
1105	74-75	0.2550037036623678
1105	76-77	0.31234011561328145
1105	78-79	0.31339368971011083
1105	80-81	0.28706480849590577
1105	82-83	0.25814089818803865
1105	84-85	0.20732236236227664
1105	86-87	0.20922083909098887
1105	88-89	0.18190440409924946
1105	90-91	0.2048322978866821
1105	92-93	0.2008386849346735
1105	94-95	0.24437919830110388
1105	96-97	0.25482389107491343
1105	98-99	0.30842496430740596
1105	100	0.4775972699856652
1106	1	0.11616109871448188
1106	2	0.05249990893185341
1106	3	0.15227105615867487
1106	4	0.13707361072088986
1106	5	0.10544679664103995
1106	6	0.04813474682484298
1106	7	0.19215356738223477
1106	8	0.18891226909474312
1106	9	0.1987101790280903
1106	10-11	0.2120226718223961
1106	12-13	0.18183017860668116
1106	14-15	0.253953168936377
1106	16-17	0.25720902125797807
1106	18-19	0.24727473255223487
1106	20-21	0.2922468661262059
1106	22-23	0.2979428850001966
1106	24-25	0.3109472977594052
1106	26-27	0.26760546477543556
1106	28-29	0.2004166058982122
1106	30-31	0.23455734931825845
1106	32-33	0.27959501555191224
1106	34-35	0.2200100908455056
1106	36-37	0.26086291627231617
1106	38-39	0.20509927567952246
1106	40-41	0.17542688551438346
1106	42-43	0.26665352961731514
1106	44-45	0.22679998514218624
1106	46-47	0.2941957517484397
1106	48-49	0.26590002662747025
1106	50-51	0.23347222083109642
1106	52-53	0.166363429581466
1106	54-55	0.18194789920133303
1106	56-57	0.19897903700681496
1106	58-59	0.1597226336888653
1106	60-61	0.21214218932134088
1106	62-63	0.19542029892969737
1106	64-65	0.18936244713376738
1106	66-67	0.18746930966802466
1106	68-69	0.12000511523935842
1106	70-71	0.10130708953308698
1106	72-73	0.18255461323801114
1106	74-75	0.14903124972037318
1106	76-77	0.1377294470939745
1106	78-79	0.14972842096869954
1106	80-81	0.13214586956681273
1106	82-83	0.19028362855411984
1106	84-85	0.20992128628100204
1106	86-87	0.18984402301366998
1106	88-89	0.10788147271590987
1106	90-91	0.1488844386393282
1106	92-93	0.13985423790961704
1106	94-95	0.1960772464458529
1106	96-97	0.1832523154424237
1106	98-99	0.1737332600725594
1106	100	0.18043504135135535
1107	1	0.024849455567991896
1107	2	0.08050193555584784
1107	3	0.08257508868527097
1107	4	0.05005461950532464
1107	5	0.09813846352729172
1107	6	0.06191379869539304
1107	7	0.029852516699861553
1107	8	0.06104503632284519
1107	9	0.08108446425984539
1107	10-11	0.16823293585844823
1107	12-13	0.15081898366509705
1107	14-15	0.187804242615897
1107	16-17	0.12400187531230245
1107	18-19	0.17751906381422344
1107	20-21	0.16638510925161398
1107	22-23	0.20369251728531879
1107	24-25	0.28215960997928136
1107	26-27	0.25932360803091825
1107	28-29	0.19573194685494144
1107	30-31	0.2446111919985796
1107	32-33	0.2817453091249362
1107	34-35	0.2473866578259063
1107	36-37	0.25727369121644017
1107	38-39	0.19706928715414307
1107	40-41	0.2187116124112336
1107	42-43	0.2557659278968458
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2216	66-67	-0.668587619833481
2216	68-69	-0.7836685473171556
2216	70-71	-0.7504251992888982
2216	72-73	-0.6626099406524588
2216	74-75	-0.6512441733642262
2216	76-77	-0.756522479001049
2216	78-79	-0.6019301537555961
2216	80-81	-0.6914679155264167
2216	82-83	-0.6016448056994435
2216	84-85	-0.6578407705726335
2216	86-87	-0.6442636871768848
2216	88-89	-0.6499011072744949
2216	90-91	-0.6607300503279419
2216	92-93	-0.5636009617717406
2216	94-95	-0.6528765913987904
2216	96-97	-0.6864595748808711
2216	98-99	-0.6250039814577022
2216	100	-0.668411077873106
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	987.0
1	271.0
2	192.0
3	180.0
4	133.0
5	166.0
6	230.0
7	520.0
8	1556.0
9	2250.0
10	2522.0
11	3337.0
12	4707.0
13	6452.0
14	8750.0
15	9796.0
16	10582.0
17	11766.0
18	12983.0
19	14695.0
20	16253.0
21	18339.0
22	20200.0
23	23188.0
24	28424.0
25	34038.0
26	36856.0
27	34805.0
28	37082.0
29	45423.0
30	52169.0
31	60555.0
32	76673.0
33	103304.0
34	151556.0
35	233234.0
36	432044.0
37	847028.0
38	883430.0
39	239969.0
40	296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.504371666106564	19.248063036064657	13.067678541938776	24.17988675589
2	15.190243139364634	20.910048854148418	39.0401993995041	24.85950860698285
3	18.533815118501717	25.782790247371935	30.002066772930352	25.68132786119599
4	12.567925859961232	17.282936735315385	38.64273848201128	31.506398922712098
5	13.352354488595244	38.588995538091915	34.61216155011254	13.446488423200305
6	30.528725889747804	37.823905845699585	17.80197122864168	13.845397035910933
7	26.89741657065639	33.00121621032558	22.48389813163189	17.61746908738614
8	25.890753347740663	34.71609808145433	20.401168829594184	18.991979741210827
9	26.13939536861259	16.203043968074965	20.37540290911421	37.28215775419824
10-11	24.786793678285342	26.304019629068343	28.038940607635134	20.870246085011185
12-13	25.563207509048613	24.27794390149569	27.54014870317668	22.618699886279014
14-15	22.946667707765187	25.50362780015931	25.237195101621847	26.312509390453652
16-17	22.590283758543205	28.288720972810555	26.04045833555244	23.0805369330938
18-19	22.605359339528324	28.303877475408466	26.902146105731646	22.188617079331564
20-21	23.865568096653995	27.056575220348762	26.18189097071219	22.89596571228505
22-23	23.68209236657692	26.900107594310995	26.15491415907938	23.262885880032705
24-25	23.683396207788505	27.05848291334121	26.209266431333678	23.04885444753661
26-27	23.35558844769928	26.993940289598388	26.314163974135536	23.336307288566793
28-29	23.04929223544599	27.77171580186807	26.122959387359394	23.056032575326547
30-31	23.5101613222399	27.031257690508465	25.968626983438515	23.48995400381312
32-33	23.362573935073684	27.465207321322428	25.635266434739535	23.536952308864358
34-35	23.813417668897266	27.290469811832768	25.9414712725971	22.954641246672864
36-37	23.64926922668876	26.80894090374693	26.33649132100691	23.205298548557398
38-39	23.480161961319183	27.14372053459187	26.227877803530603	23.148239700558342
40-41	23.334486609510936	26.99888814938723	26.37759128094342	23.289033960158424
42-43	23.169113430199214	27.111606511980916	25.95938088171251	23.759899176107357
44-45	23.19422364037216	27.13965521173471	26.165862341623697	23.500258806269432
46-47	23.60602318305398	27.165739725560766	25.947581494895328	23.280655596489922
48-49	23.449708916690845	27.037806907761308	26.041036034280417	23.47144814126743
50-51	23.025796328667543	27.135468616704173	26.318678701392805	23.520056353235475
52-53	23.173363732701326	27.34329223480827	25.936460143342238	23.54688388914817
54-55	22.88353122997692	27.04278889130583	26.125243813351233	23.94843606536601
56-57	23.415478929061404	26.866587099217952	25.980417630945617	23.737516340775024
58-59	23.47344454382775	26.71930388828675	26.136453532787733	23.670798035097768
60-61	23.01961302869602	26.999485508786194	26.480259359005153	23.50064210351263
62-63	23.041856173304126	26.921606687148252	26.24342483064581	23.793112308901808
64-65	22.725920318266134	27.181522016713362	26.087243990320243	24.005313674700265
66-67	23.12531688404383	26.74024921089377	25.97155637273302	24.16287753232939
68-69	22.831963854358982	26.648633112302473	26.28396323014277	24.235439803195774
70-71	23.12698852435847	26.55951775482743	26.043604001944292	24.26988971886981
72-73	23.057218951730547	26.44738564936258	26.596293712239756	23.89910168666712
74-75	22.862248038648413	27.180671866139562	26.32218715099606	23.634892944215963
76-77	22.98599018738554	26.529947587715437	26.239259061856774	24.24480316304225
78-79	22.903704469409888	26.562436837260446	26.39970074576788	24.13415794756179
80-81	23.019104846755702	26.654463964372997	26.446549319960887	23.879881868910406
82-83	23.073880178338733	26.179764542284417	27.00802154709804	23.738333732278807
84-85	22.98174593584579	26.655671755710863	26.514048756135733	23.84853355230761
86-87	22.731221670857483	26.90172474474719	26.451302386266946	23.915751198128383
88-89	22.87181674032824	26.34285602098171	26.259633074141615	24.52569416454844
90-91	22.846806306545606	26.16760827537782	26.568913169510356	24.416672248566222
92-93	23.152662072055634	26.26607786664696	26.60528938427747	23.97597067701994
94-95	22.56775463835082	26.082277046641174	26.95637864359364	24.39358967141436
96-97	22.424288965627643	26.063727745284176	27.040287172244597	24.47169611684358
98-99	22.482963503647525	26.46650729411656	26.495727359466137	24.554801842769773
100	22.65598321551986	25.81362715426322	26.44344996708047	25.086939663136455
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2229.0
1	1603.5
2	1034.0
3	1081.5
4	1036.5
5	931.0
6	818.5
7	757.0
8	726.0
9	732.5
10	696.5
11	635.0
12	653.0
13	710.5
14	770.5
15	834.5
16	947.5
17	1116.0
18	1379.0
19	1667.5
20	2133.0
21	2915.0
22	4090.0
23	5739.5
24	7635.0
25	10030.5
26	13570.5
27	18001.0
28	22150.5
29	27217.0
30	33511.5
31	40203.0
32	48824.0
33	54160.0
34	57037.0
35	61988.0
36	67373.5
37	75408.5
38	81179.0
39	83795.0
40	87491.0
41	90286.5
42	93446.5
43	98975.5
44	105888.0
45	116935.0
46	128600.0
47	143165.0
48	165842.5
49	166585.5
50	148832.0
51	143681.0
52	143099.5
53	142189.0
54	138370.5
55	127907.5
56	117801.5
57	104742.0
58	90783.5
59	80431.5
60	67309.0
61	54166.0
62	44437.5
63	34544.0
64	24773.0
65	17727.5
66	13100.5
67	9692.0
68	7197.0
69	5405.0
70	4102.0
71	3037.0
72	2320.5
73	2023.5
74	1594.0
75	1137.0
76	853.5
77	611.5
78	412.0
79	284.0
80	210.0
81	162.0
82	119.0
83	93.5
84	71.5
85	54.0
86	47.5
87	34.0
88	28.5
89	25.0
90	19.0
91	16.0
92	13.5
93	9.5
94	11.0
95	10.5
96	6.0
97	4.0
98	5.5
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09440599075669301
2	0.061668196834039
3	0.07508059698737302
4	0.0774746383050649
5	0.07868608089955959
6	0.08682005260545247
7	0.08370491450532329
8	0.08546439065447033
9	0.07066748467885665
10-11	0.07761885766155235
12-13	0.06694662528148013
14-15	0.07647952474530141
16-17	0.07818131315185346
18-19	0.07757559185460612
20-21	0.06854746013849097
22-23	0.07322016728868475
24-25	0.07585938151240532
26-27	0.0697733246686344
28-29	0.07857070541436961
30-31	0.08219061126220492
32-33	0.08428179193127314
34-35	0.08656045776377504
36-37	0.085767251303094
38-39	0.08732482035315858
40-41	0.08354627321318707
42-43	0.0888247016606282
44-45	0.08537785904057785
46-47	0.0824646280395311
48-49	0.09103125781488638
50-51	0.08872374811108698
52-53	0.09439156882104426
54-55	0.08565187581790402
56-57	0.09267535847884346
58-59	0.09688656368827736
60-61	0.09590587206416262
62-63	0.09639621787621999
64-65	0.09877583725826312
66-67	0.10179002180885109
68-69	0.09799705273323081
70-71	0.10021803082313774
72-73	0.09791052111933835
74-75	0.1013285198680912
76-77	0.09922291726337425
78-79	0.10571278830531006
80-81	0.10096797147687255
82-83	0.10510706700806273
84-85	0.10154484890282241
86-87	0.09847297660963944
88-89	0.0967135004604924
90-91	0.09705962691606232
92-93	0.09152160362694375
94-95	0.09103125781488638
96-97	0.08931504747268557
98-99	0.10290051085380454
100	0.24540365699906633
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3466941.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.690573709828094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.45682457711276	36.75486185898244
2	15.629475504627777	16.783110132543406
3	6.448913402370541	10.387375811348214
4	3.30920853572196	7.106932192334889
5	1.9294057227520138	5.179545008679058
6	1.152816484879003	3.7137227073180625
7	0.751952699157918	2.826094027430967
8	0.549597374521169	2.3606558677965452
9	0.3636836382904445	1.7573744869822412
>10	1.3837102396076646	11.414758612174653
>50	0.020494983833101613	0.7109561669867464
>100	0.0035614143962010283	0.24225325978604315
>500	8.885568236274567E-5	0.04336577720161568
>1k	1.7771136472549134E-4	0.23290618734685117
>5k	0.0	0.0
>10k+	8.885568236274567E-5	0.4860879030882972
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	10189	0.29389020465015125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3075097037994014E-4
2	0.0	0.0	0.0	0.0	2.3075097037994014E-4
3	0.0	0.0	0.0	0.0	2.3075097037994014E-4
4	0.0	0.0	0.0	0.0	2.3075097037994014E-4
5	0.0	0.0	0.0	0.0	3.1728258427241767E-4
6	0.0	0.0	0.0	0.0	4.615019407598803E-4
7	0.0	0.0	0.0	0.0	4.615019407598803E-4
8	0.0	0.0	0.0	0.0	4.615019407598803E-4
9	0.0	0.0	0.0	0.0	5.191896833548653E-4
10-11	0.0	0.0	0.0	0.0	5.191896833548653E-4
12-13	0.0	0.0	0.0	1.4421935648746259E-5	0.0010528013023584767
14-15	0.0	0.0	0.0	2.8843871297492517E-5	0.0013556619509821483
16-17	0.0	0.0	0.0	7.210967824373129E-5	0.0016585225996058198
18-19	0.0	0.0	0.0	1.4421935648746258E-4	0.001846007763039521
20-21	0.0	0.0	0.0	2.0190709908244763E-4	0.0020334929264732224
22-23	0.0	0.0	0.0	5.624554903011041E-4	0.002134446476014446
24-25	0.0	0.0	0.0	0.0013700838866308944	0.0021632903473119388
26-27	0.0	0.0	0.0	0.002437307124638117	0.002336353575096894
28-29	2.8843871297492517E-5	0.0	0.0	0.007254233631319367	0.002408463253340625
30-31	2.8843871297492517E-5	0.0	0.0	0.020104178294352282	0.0024228851889893715
32-33	2.8843871297492517E-5	0.0	0.0	0.04630883536812423	0.0025382606741793413
34-35	2.8843871297492517E-5	0.0	0.0	0.08360396095578207	0.0026680580950180577
36-37	2.8843871297492517E-5	0.0	0.0	0.1427915848582367	0.002682480030666804
38-39	2.8843871297492517E-5	0.0	0.0	0.23103940909291504	0.002841121322803013
40-41	2.8843871297492517E-5	0.0	0.0	0.330565187004913	0.002898809065397998
42-43	2.8843871297492517E-5	0.0	0.0	0.43206677010078914	0.003028606486236714
44-45	2.8843871297492517E-5	0.0	0.0	0.5587057870324301	0.0032449355209679077
46-47	2.8843871297492517E-5	0.0	0.0	0.7144771139745383	0.003533374233942833
48-49	2.8843871297492517E-5	0.0	0.0	0.8814398629800738	0.003980454239053967
50-51	2.8843871297492517E-5	0.0	0.0	1.065247432823345	0.004211205209433908
52-53	2.8843871297492517E-5	0.0	0.0	1.2435314013131462	0.004514065858057578
54-55	2.8843871297492517E-5	0.0	0.0	1.4422224087459232	0.004744816828437519
56-57	2.8843871297492517E-5	0.0	0.0	1.6800978153363442	0.004845770377978743
58-59	2.8843871297492517E-5	0.0	0.0	1.9467882493529598	0.004917880056222474
60-61	2.8843871297492517E-5	0.0	0.0	2.236496092664975	0.00504767747706119
62-63	2.8843871297492517E-5	0.0	0.0	2.531237191518402	0.005364960061333608
64-65	2.8843871297492517E-5	0.0	0.0	2.828112736847844	0.005422647803928593
66-67	2.8843871297492517E-5	0.0	0.0	3.171325961416707	0.005509179417821071
68-69	2.8843871297492517E-5	0.0	0.0	3.5568387232433434	0.005595711031713548
70-71	5.7687742594985034E-5	0.0	0.0	3.97800250999368	0.005667820709957279
72-73	5.7687742594985034E-5	0.0	0.0	4.404358193577566	0.005999525229878443
74-75	5.7687742594985034E-5	0.0	0.0	4.829762029408634	0.006143744586365906
76-77	5.7687742594985034E-5	0.0	0.0	5.298287452829454	0.00631680781415086
78-79	5.7687742594985034E-5	0.0	0.0	5.828654136312098	0.0064466052349895775
80-81	5.7687742594985034E-5	0.0	0.0	6.4081130887430735	0.006662934269720771
82-83	5.7687742594985034E-5	0.0	0.0	6.992172638646	0.006908107175749457
84-85	5.7687742594985034E-5	0.0	0.0	7.580371284080115	0.006922529111398203
86-87	5.7687742594985034E-5	0.0	0.0	8.1787085502753	0.00716770201742689
88	5.7687742594985034E-5	0.0	0.0	8.652209541494937	0.007499406537348054
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	11975	0.0	26.156418	1
GTACTAG	1180	0.0	25.508066	1
CTAACGC	610	0.0	25.435013	3
TACATGG	11930	0.0	25.025406	2
ACATGGG	12230	0.0	23.488894	3
TCTAACG	630	0.0	22.388683	2
ATACGGG	915	0.0	21.067383	3
CATGGGG	8975	0.0	20.587313	4
GGCGTCG	970	0.0	20.350197	8
TAACGCC	725	0.0	20.103203	4
TACGGGC	985	0.0	20.047241	4
TAGTACT	1685	0.0	19.810709	4
TGATCGC	970	0.0	19.37918	9
CGGGCGT	1000	0.0	19.269419	6
CTAGTAC	1715	0.0	18.916153	3
GTGATCG	1005	0.0	18.23852	8
GAGTACA	10180	0.0	17.92518	1
GTATAAG	1710	0.0	17.602058	1
GTCCTAC	1225	0.0	17.27651	1
CCTAGTA	1905	0.0	17.276306	2
>>END_MODULE