FastQCFastQC Report
Mon 16 Jan 2017
ERR1146523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466941
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144350.41636128217930446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124780.35991382605011163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74400.2145984024533443No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11150.024.8861941
GTACATG114200.023.9271721
TACATGG114700.023.3711592
GTATCAA275250.023.0497381
GAGTACT158000.022.96364412-13
ACATGGG116250.022.371483
GGTATCA208050.021.6334381
AGTACTT165200.021.49339512-13
AGAGTAC223350.021.45269610-11
CATGGGG88350.021.4516684
TCTAACG5500.021.376972
GTACTTT172500.020.76091414-15
CAACGCA312450.019.9321215
ATCAACG315750.019.779593
TATCAAC320000.019.6788372
AACGCAG322950.019.457886
TCAACGC322600.019.388754
TACTTTT185500.018.97660414-15
ATACGGG10000.018.811463
CTAGTAC16550.018.754633
Produced by FastQC (version 0.11.5)