##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146523_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3466941
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.303582610722245	33.0	31.0	34.0	30.0	34.0
2	32.35152891266393	34.0	31.0	34.0	30.0	34.0
3	32.46666153245758	34.0	31.0	34.0	30.0	34.0
4	36.00290486627837	37.0	35.0	37.0	35.0	37.0
5	36.045748110510104	37.0	35.0	37.0	35.0	37.0
6	36.13095896353587	37.0	36.0	37.0	35.0	37.0
7	36.10392591047843	37.0	36.0	37.0	35.0	37.0
8	36.110554232102594	37.0	36.0	37.0	35.0	37.0
9	37.88748294245561	39.0	38.0	39.0	35.0	39.0
10-11	37.84426602587122	39.0	38.0	39.0	35.0	39.0
12-13	37.81853282187381	39.0	38.0	39.0	35.0	39.0
14-15	39.156973106839715	40.0	39.0	41.0	36.0	41.0
16-17	39.115780597362345	40.0	39.0	41.0	36.0	41.0
18-19	39.231347317419015	40.5	39.0	41.0	36.0	41.0
20-21	39.28511863916923	41.0	39.0	41.0	36.0	41.0
22-23	39.24752584482978	41.0	39.0	41.0	36.0	41.0
24-25	39.23129222562484	41.0	39.0	41.0	36.0	41.0
26-27	39.145145821633534	41.0	39.0	41.0	35.5	41.0
28-29	39.08309616460159	40.0	39.0	41.0	35.0	41.0
30-31	38.93011591486558	40.0	38.5	41.0	35.0	41.0
32-33	38.800953924511546	40.0	38.0	41.0	35.0	41.0
34-35	38.715058173761825	40.0	38.0	41.0	35.0	41.0
36-37	38.629447256241164	40.0	38.0	41.0	35.0	41.0
38-39	38.53328525059988	40.0	38.0	41.0	35.0	41.0
40-41	38.43206028022975	40.0	38.0	41.0	34.0	41.0
42-43	38.30639214800598	40.0	38.0	41.0	34.0	41.0
44-45	38.13832467872975	40.0	38.0	41.0	33.5	41.0
46-47	37.86245973035018	40.0	37.5	41.0	33.0	41.0
48-49	37.54064678343242	40.0	37.0	41.0	33.0	41.0
50-51	37.40830937128725	40.0	37.0	41.0	32.0	41.0
52-53	37.295679678425444	40.0	36.5	41.0	32.0	41.0
54-55	37.12119473622424	40.0	36.0	41.0	32.0	41.0
56-57	36.944897389370055	39.0	35.5	41.0	31.0	41.0
58-59	36.74430715723169	39.0	35.0	41.0	31.0	41.0
60-61	36.5101192953673	39.0	35.0	41.0	31.0	41.0
62-63	36.265086137895054	38.0	35.0	40.5	31.0	41.0
64-65	35.97704792207309	37.0	35.0	40.0	31.0	41.0
66-67	35.68570621767143	37.0	35.0	40.0	30.5	41.0
68-69	35.37403679497286	36.0	35.0	39.0	30.0	41.0
70-71	35.05179046888885	36.0	35.0	39.0	30.0	41.0
72-73	34.72981787114347	35.0	35.0	39.0	30.0	40.5
74-75	34.43213008239829	35.0	34.0	37.0	29.0	40.0
76-77	34.158410685385185	35.0	34.0	37.0	29.0	39.0
78-79	33.89497946460583	35.0	34.0	36.5	29.0	39.0
80-81	33.661053505092816	35.0	34.0	36.0	29.0	38.5
82-83	33.43396037025147	35.0	34.0	36.0	29.0	37.0
84-85	33.252349405426855	35.0	34.0	35.0	29.0	37.0
86-87	33.100379123844334	35.0	34.0	35.0	29.0	36.5
88-89	32.923392696904855	35.0	34.0	35.0	29.0	36.0
90-91	32.85464232013178	35.0	34.0	35.0	29.0	36.0
92-93	32.76605010007381	35.0	34.0	35.0	29.0	36.0
94-95	32.70273217802091	35.0	34.0	35.0	29.0	35.5
96-97	32.65682802216709	35.0	34.0	35.0	28.5	35.0
98-99	32.597940662964845	35.0	34.0	35.0	27.0	35.0
100	31.86291084849728	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22285133279441993
1101	2	0.25208788055998355
1101	3	0.20755307090447417
1101	4	0.1219408585507864
1101	5	0.13391203637763738
1101	6	0.16859170928268696
1101	7	0.2000175798223509
1101	8	0.20617107371754173
1101	9	0.25495367502443855
1101	10-11	0.24710400866308646
1101	12-13	0.2890551242797983
1101	14-15	0.4370379883522446
1101	16-17	0.46889925577020364
1101	18-19	0.37874928088456983
1101	20-21	0.3703165896529441
1101	22-23	0.29313441477454205
1101	24-25	0.32504319285533256
1101	26-27	0.36082377376749974
1101	28-29	0.36917682859625955
1101	30-31	0.4820794790328691
1101	32-33	0.4608961869008965
1101	34-35	0.43932710514061313
1101	36-37	0.4550094303851395
1101	38-39	0.3853574714759276
1101	40-41	0.3822629880020614
1101	42-43	0.3739298992218636
1101	44-45	0.38906871987926195
1101	46-47	0.4466263772664263
1101	48-49	0.5613546327056582
1101	50-51	0.5124068599265925
1101	52-53	0.4527045779292109
1101	54-55	0.49608965355448476
1101	56-57	0.4159101180823015
1101	58-59	0.4083493388130961
1101	60-61	0.42958264204569474
1101	62-63	0.40709918256776945
1101	64-65	0.3887974444572393
1101	66-67	0.40910959909840017
1101	68-69	0.40377940255455513
1101	70-71	0.375132615912122
1101	72-73	0.3472188663789666
1101	74-75	0.36770726695974076
1101	76-77	0.33369187933685396
1101	78-79	0.30395696742206724
1101	80-81	0.2757563453871157
1101	82-83	0.33387160299465535
1101	84-85	0.3378951021051506
1101	86-87	0.40339893872067023
1101	88-89	0.462814340878694
1101	90-91	0.4228829975391406
1101	92-93	0.3723832274321026
1101	94-95	0.3484171408506853
1101	96-97	0.3035607470574959
1101	98-99	0.35745704208876816
1101	100	0.3697959108973201
1102	1	0.23027661961750567
1102	2	0.21216311612590033
1102	3	0.2206981683835565
1102	4	0.19193188914849202
1102	5	0.13996799509162372
1102	6	0.16419204372714802
1102	7	0.17591790559481524
1102	8	0.2184889149164846
1102	9	0.24436113719370667
1102	10-11	0.20564660639409738
1102	12-13	0.21292011566988833
1102	14-15	0.291972006603082
1102	16-17	0.4208332356178843
1102	18-19	0.36704059675335543
1102	20-21	0.26059637167307415
1102	22-23	0.30283865068281557
1102	24-25	0.3434307563108021
1102	26-27	0.33943212465737105
1102	28-29	0.3143228554513371
1102	30-31	0.3376025865289307
1102	32-33	0.3384231805283733
1102	34-35	0.33484377072127813
1102	36-37	0.3567671165777142
1102	38-39	0.3466367282677325
1102	40-41	0.3653424208709666
1102	42-43	0.3414778721456031
1102	44-45	0.37665737321479753
1102	46-47	0.37187347376050184
1102	48-49	0.38039111831692907
1102	50-51	0.2909686650180845
1102	52-53	0.27385653446490466
1102	54-55	0.3334132672601555
1102	56-57	0.27444306208383296
1102	58-59	0.28025503217465086
1102	60-61	0.24144781669301807
1102	62-63	0.22167072204502603
1102	64-65	0.18262309040291314
1102	66-67	0.13193577027240622
1102	68-69	0.12842019575040098
1102	70-71	0.21358491229182874
1102	72-73	0.1732685987743139
1102	74-75	0.2661293393700248
1102	76-77	0.18736903590831133
1102	78-79	0.16673466493924138
1102	80-81	0.14074193649805267
1102	82-83	0.14158358055873776
1102	84-85	0.19062303142253256
1102	86-87	0.2531811330663203
1102	88-89	0.2767526130266731
1102	90-91	0.1977707308251766
1102	92-93	0.23919364291110412
1102	94-95	0.24830024217529
1102	96-97	0.20119861035124842
1102	98-99	0.21635950833271522
1102	100	0.23068793090123663
1103	1	0.20663075062863356
1103	2	0.2069636250557636
1103	3	0.22325720242465508
1103	4	0.16733011428114963
1103	5	0.12342524139602773
1103	6	0.21454306953692281
1103	7	0.22319964663903846
1103	8	0.22777045701721477
1103	9	0.21591266896592742
1103	10-11	0.2478368549147092
1103	12-13	0.24283720580771728
1103	14-15	0.29263304817770575
1103	16-17	0.4025978563254
1103	18-19	0.3193916380335011
1103	20-21	0.28403741661227855
1103	22-23	0.3108404948230259
1103	24-25	0.3490257369273806
1103	26-27	0.3474096776061728
1103	28-29	0.331498876435667
1103	30-31	0.4272635000432885
1103	32-33	0.3995735860216385
1103	34-35	0.3844584613100821
1103	36-37	0.3881044271827676
1103	38-39	0.39955870753933453
1103	40-41	0.40024709875280706
1103	42-43	0.43047650012302086
1103	44-45	0.46222110459330423
1103	46-47	0.5699939914532948
1103	48-49	0.6068052874729162
1103	50-51	0.5065729599721323
1103	52-53	0.4921625120114328
1103	54-55	0.47523284215164097
1103	56-57	0.48179902741917147
1103	58-59	0.4081602552738559
1103	60-61	0.3749289538591327
1103	62-63	0.3009549542708072
1103	64-65	0.3726751101928585
1103	66-67	0.3436970811354598
1103	68-69	0.35169616462854236
1103	70-71	0.35458157218515396
1103	72-73	0.30480922258025345
1103	74-75	0.3184466851036518
1103	76-77	0.27080059326002015
1103	78-79	0.30286573586549537
1103	80-81	0.29598807163153396
1103	82-83	0.368408053721474
1103	84-85	0.30377517810116217
1103	86-87	0.3530639563396676
1103	88-89	0.37818923570245744
1103	90-91	0.3497338232322633
1103	92-93	0.40709318178105747
1103	94-95	0.33660168581843664
1103	96-97	0.3693543828407755
1103	98-99	0.41464728241323456
1103	100	0.31349004332114205
1104	1	0.15591384485916393
1104	2	0.18475812346245846
1104	3	0.1344493742233226
1104	4	0.15421830367548495
1104	5	0.10563848915431606
1104	6	0.17107437935242586
1104	7	0.1616833858179021
1104	8	0.1508338707971788
1104	9	0.1848186101168352
1104	10-11	0.2405090745130849
1104	12-13	0.206819594719164
1104	14-15	0.35706962690172617
1104	16-17	0.34230869272360565
1104	18-19	0.2740251716974029
1104	20-21	0.2882491689375257
1104	22-23	0.29219525641428135
1104	24-25	0.2853018790562345
1104	26-27	0.2914901537665884
1104	28-29	0.2896990770203871
1104	30-31	0.3680009260394428
1104	32-33	0.3489690990200245
1104	34-35	0.32057185430253554
1104	36-37	0.3316550423569353
1104	38-39	0.37752120229105657
1104	40-41	0.3446039635966187
1104	42-43	0.3608113028746658
1104	44-45	0.3601762823803014
1104	46-47	0.3761102534909284
1104	48-49	0.49004654264633984
1104	50-51	0.37495065070859823
1104	52-53	0.3670581568111331
1104	54-55	0.3734764664933081
1104	56-57	0.36416946013098084
1104	58-59	0.34207521776852445
1104	60-61	0.2840345142468763
1104	62-63	0.32143699412509363
1104	64-65	0.29927886918175517
1104	66-67	0.29721021796133584
1104	68-69	0.27002938045296077
1104	70-71	0.3584816831518367
1104	72-73	0.40927463567949474
1104	74-75	0.37829206335800336
1104	76-77	0.30895176221388
1104	78-79	0.3650614566619126
1104	80-81	0.353382211017788
1104	82-83	0.35774538310045756
1104	84-85	0.3481131487104534
1104	86-87	0.3713728593834986
1104	88-89	0.41624162863091385
1104	90-91	0.37595688759696344
1104	92-93	0.3854432203705329
1104	94-95	0.347168514567457
1104	96-97	0.34000154057134324
1104	98-99	0.31617426724379527
1104	100	0.4332659545299755
1105	1	0.16488437818919266
1105	2	0.11012442044322768
1105	3	0.14839138991401768
1105	4	0.14704999414849595
1105	5	0.11883379324022769
1105	6	0.12319233053503353
1105	7	0.12103802060970992
1105	8	0.11986020842864065
1105	9	0.19831913968080528
1105	10-11	0.1725673172057327
1105	12-13	0.166599140102619
1105	14-15	0.24865534902831854
1105	16-17	0.32661180319588823
1105	18-19	0.24720713410263073
1105	20-21	0.21264337985064685
1105	22-23	0.26115521065280234
1105	24-25	0.21725617933098818
1105	26-27	0.2501471129115487
1105	28-29	0.23124600101952808
1105	30-31	0.2561837476911464
1105	32-33	0.258833390889194
1105	34-35	0.22277203313955596
1105	36-37	0.16672351191097334
1105	38-39	0.2339200697919992
1105	40-41	0.19155174944232556
1105	42-43	0.20173942721068272
1105	44-45	0.23185444155337365
1105	46-47	0.28418422506632623
1105	48-49	0.28596164828084625
1105	50-51	0.24934985865802162
1105	52-53	0.14947840025211434
1105	54-55	0.15463359728443749
1105	56-57	0.22466601928553587
1105	58-59	0.19511528465568517
1105	60-61	0.21076286925908505
1105	62-63	0.22757162809624276
1105	64-65	0.23586001881699303
1105	66-67	0.20657972198313246
1105	68-69	0.21701399405001354
1105	70-71	0.19746165397401683
1105	72-73	0.20451806650649473
1105	74-75	0.16246401178371173
1105	76-77	0.14103373067242586
1105	78-79	0.1811858568652056
1105	80-81	0.19132864847622244
1105	82-83	0.12532217393873424
1105	84-85	0.19852483543770916
1105	86-87	0.10843039314701741
1105	88-89	0.1557088511196909
1105	90-91	0.12952418244119457
1105	92-93	0.09820274632647852
1105	94-95	0.09561537068002224
1105	96-97	0.08951237946811119
1105	98-99	0.10370922110652003
1105	100	0.12553407681029327
1106	1	0.06147357579361312
1106	2	0.08014627675950692
1106	3	0.04070922436383029
1106	4	0.062089269593862184
1106	5	0.04673026225344756
1106	6	0.04849849645033544
1106	7	0.09993323706837742
1106	8	0.06408451204380583
1106	9	0.06310410091246155
1106	10-11	0.0874990859446001
1106	12-13	0.11029123480993519
1106	14-15	0.2124385201719008
1106	16-17	0.291067398362145
1106	18-19	0.20161440196167035
1106	20-21	0.14994668568186853
1106	22-23	0.1635872963336169
1106	24-25	0.16317371357339283
1106	26-27	0.18141939393002104
1106	28-29	0.20017706995059825
1106	30-31	0.19160593971232487
1106	32-33	0.17547284280783515
1106	34-35	0.19307457323905197
1106	36-37	0.14023401156359938
1106	38-39	0.17812637110490215
1106	40-41	0.16192148123338512
1106	42-43	0.13992154965676207
1106	44-45	0.1107717171711542
1106	46-47	0.10789198938321931
1106	48-49	0.2325787367761052
1106	50-51	0.17633835239779216
1106	52-53	0.1768974279249491
1106	54-55	0.20529740048630885
1106	56-57	0.12923226522234188
1106	58-59	0.06509327620727845
1106	60-61	0.13190999624377753
1106	62-63	0.17309691499208668
1106	64-65	0.1703389916208451
1106	66-67	0.11503674719142509
1106	68-69	0.13486695327353715
1106	70-71	0.0734262984665861
1106	72-73	0.12652981110707628
1106	74-75	0.10619388597044122
1106	76-77	0.03825858703433482
1106	78-79	0.07045521694675472
1106	80-81	0.11427296304490397
1106	82-83	0.029560575131448275
1106	84-85	0.05811068106416428
1106	86-87	0.06129504118932516
1106	88-89	0.11145614950658
1106	90-91	0.05371674115152558
1106	92-93	0.08471597928640762
1106	94-95	0.11461794171305684
1106	96-97	0.12251186728993702
1106	98-99	0.14280210791514492
1106	100	0.15132148736927675
1107	1	0.11963832822688403
1107	2	0.05165436289341585
1107	3	0.07660431962234071
1107	4	0.04667241926938459
1107	5	0.07902643801875797
1107	6	0.07661348759882713
1107	7	0.06205149333330695
1107	8	0.04475904425539312
1107	9	0.07832908372449054
1107	10-11	0.13717439341173332
1107	12-13	0.15016168568587318
1107	14-15	0.18947674006059856
1107	16-17	0.2566367245935126
1107	18-19	0.21567942101816584
1107	20-21	0.1529905518504222
1107	22-23	0.15922813083078324
1107	24-25	0.1366368944747336
1107	26-27	0.19295551233421548
1107	28-29	0.16203997698221428
1107	30-31	0.16105985318075255
1107	32-33	0.12589882162410504
1107	34-35	0.11917020934985345
1107	36-37	0.12173567765933058
1107	38-39	0.13669687504197015
1107	40-41	0.04909776937331145
1107	42-43	0.09615015452018127
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2216	48-49	-0.5694065764011782
2216	50-51	-0.5924653808441818
2216	52-53	-0.6508233026710215
2216	54-55	-0.6227580164988424
2216	56-57	-0.5439588793316545
2216	58-59	-0.5238833728163712
2216	60-61	-0.6437784259915347
2216	62-63	-0.6754693555923836
2216	64-65	-0.6597354420059176
2216	66-67	-0.5870479302412761
2216	68-69	-0.6275537743419548
2216	70-71	-0.6290643258287503
2216	72-73	-0.5710938312333838
2216	74-75	-0.5746440613305239
2216	76-77	-0.5341050087685488
2216	78-79	-0.5419750993720811
2216	80-81	-0.5661923193762775
2216	82-83	-0.500900793533269
2216	84-85	-0.5166560515242082
2216	86-87	-0.4957783510771492
2216	88-89	-0.4164112415863741
2216	90-91	-0.4926444738831961
2216	92-93	-0.434319704237609
2216	94-95	-0.42121883597684473
2216	96-97	-0.4800582965790454
2216	98-99	-0.5315384918590027
2216	100	-0.5846043583859668
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	26.0
8	137.0
9	286.0
10	364.0
11	255.0
12	244.0
13	333.0
14	505.0
15	943.0
16	1749.0
17	3265.0
18	5384.0
19	8076.0
20	11763.0
21	17260.0
22	24531.0
23	28143.0
24	27132.0
25	23307.0
26	21502.0
27	23742.0
28	28942.0
29	35451.0
30	43673.0
31	55076.0
32	69849.0
33	91931.0
34	132498.0
35	207677.0
36	383212.0
37	865088.0
38	1040400.0
39	313523.0
40	671.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.386133501786205	19.225174474197722	13.541332248773742	23.84735977524233
2	15.558724634269952	21.019587617863337	38.68611496429162	24.735572783575098
3	18.525577809307425	25.981249698941223	30.606896993335024	24.88627549841632
4	12.584508962776663	17.481880372218928	38.159510441782714	31.774100223221698
5	12.896013743840973	38.253786853933036	34.71620733677891	14.133992065447082
6	30.05025418429111	38.151873671676505	17.396941711838316	14.400930432194063
7	26.137746831499825	33.51488632627043	22.460209868068095	17.887156974161652
8	26.27590585212446	33.71655433382109	20.296406416168313	19.71113339788614
9	26.43640398634069	14.976150431259391	20.62868715024983	37.95875843215009
10-11	24.89251322059985	25.756258115951308	27.97242119703323	21.37880746641561
12-13	26.31476855245013	23.796467837208652	27.946033693679816	21.942729916661403
14-15	23.240991549029275	24.609345592754877	26.660001805627388	25.489661052588463
16-17	21.88827497745485	27.585229186097273	27.26630162724588	23.260194209201998
18-19	21.813734609965284	26.5312588785195	29.789799924140485	21.86520658737473
20-21	23.00435947402487	25.340733536163963	29.500809361129665	22.154097628681495
22-23	22.740984054819506	25.17990643625029	29.563987388305712	22.515122120624493
24-25	22.794849984467575	25.35262353757967	29.590538171835057	22.2619883061177
26-27	22.511541173976436	25.30898329504303	29.696792700180417	22.482682830800115
28-29	22.175067299962706	26.079445828469535	29.50804469992423	22.237442171643533
30-31	22.71728486400812	25.347807212999378	29.27479171083392	22.66011621215858
32-33	22.644242806031382	25.700258903260863	28.960562249274	22.694936041433753
34-35	23.047897212941287	25.593589956775936	29.153002457935102	22.205510372347675
36-37	22.934252414448355	25.14610141908962	29.442554690143268	22.47709147631875
38-39	22.775597725297235	25.43630654866164	29.3063760297521	22.48171969628902
40-41	22.745532961870364	25.400510106585884	29.23206388374704	22.62189304779671
42-43	22.750906346545847	25.502914528975257	28.66159245282801	23.084586671650886
44-45	22.747938839635257	25.602130537536432	28.686541088289214	22.963389534539104
46-47	23.334360175151524	25.737458468430813	28.100377248992697	22.82780410742496
48-49	23.411710852631344	25.81411897184201	27.758832145253248	23.015338030273398
50-51	22.904153834749426	26.190105340702363	27.75468345149225	23.15105737305596
52-53	22.99055648657458	26.446611267028754	27.366329865327536	23.19650238106913
54-55	22.823008489646025	26.12270109944565	27.44659610854448	23.60769430236385
56-57	23.296669349631685	25.9565255828959	27.269183404190816	23.477621663281592
58-59	23.21321255924037	25.84615970372494	27.537721953547447	23.402905783487242
60-61	22.838436273317868	26.083388641743703	27.872237139388446	23.205937945549987
62-63	22.83579670106209	26.01014325626043	27.649848540526012	23.504211502151467
64-65	22.494107174997577	26.185761573358317	27.568657324294016	23.75147392735009
66-67	22.83760957903651	25.910855910633813	27.42870872686408	23.822825783465593
68-69	22.628421074189102	25.785156411123968	27.632420373842937	23.954002140843997
70-71	22.888624093443372	25.725310204403044	27.36336425209565	24.022701450057934
72-73	22.90663654517402	25.578298910624603	27.758304443025928	23.75676010117545
74-75	22.74669761502551	26.44408808513664	27.261890871844635	23.547323427993216
76-77	22.93418752632095	25.79859929272359	27.102647382904216	24.164565798051242
78-79	22.71775282882321	25.980190954399905	27.19926239601304	24.102793820763846
80-81	22.882725539457326	26.045066067477958	27.16964097712372	23.902567415940997
82-83	22.9440008953188	25.638581663876725	27.652818213507285	23.764599227297186
84-85	22.87805683570948	26.053697479536968	27.130747802986654	23.937497881766898
86-87	22.54973801829019	26.317094279523978	27.03539331003806	24.097774392147777
88-89	22.640592960768586	25.83851297152158	26.831102692546544	24.689791375163292
90-91	22.613771421805478	25.62393268647097	27.10395520200015	24.658340689723403
92-93	22.865526130771034	25.839736078311187	27.039180833228173	24.255556957689603
94-95	22.305106784461326	25.572861577813498	27.39250814191316	24.729523495812007
96-97	22.20764321197485	25.561962916785948	27.45337557047909	24.77701830076011
98-99	22.181326366952494	25.91404544040539	26.895127730202155	25.009500462439966
100	22.313150947328435	25.212549222456932	26.97777433960652	25.496525490608114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	195.0
1	148.0
2	388.5
3	656.0
4	445.5
5	360.0
6	799.5
7	2074.5
8	3882.5
9	4655.0
10	3408.0
11	2167.5
12	2164.5
13	2377.0
14	2681.0
15	3106.0
16	3564.5
17	4017.5
18	4497.5
19	4986.0
20	5663.0
21	6518.5
22	7710.0
23	9315.0
24	11132.5
25	13302.0
26	16366.5
27	20554.0
28	24633.5
29	29617.0
30	35370.0
31	41084.0
32	49209.5
33	53937.0
34	56136.0
35	60520.0
36	65463.0
37	73252.5
38	79151.0
39	81807.0
40	85247.5
41	87736.5
42	90665.0
43	96210.5
44	102864.0
45	114005.5
46	125484.5
47	140634.0
48	164197.0
49	164980.5
50	147785.5
51	141647.0
52	140121.0
53	139581.5
54	137350.0
55	127280.5
56	116326.0
57	103842.0
58	89336.5
59	78794.0
60	66397.5
61	53261.0
62	43256.5
63	33390.5
64	23796.0
65	17045.0
66	12559.0
67	9163.0
68	6775.0
69	5016.5
70	3691.5
71	2720.5
72	2120.5
73	1804.5
74	1374.5
75	990.5
76	759.0
77	535.0
78	328.0
79	198.0
80	150.5
81	99.5
82	70.5
83	44.5
84	28.0
85	21.0
86	14.0
87	9.5
88	5.5
89	2.0
90	2.0
91	2.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0027690116445592814
2	0.0031151381001291918
3	2.8843871297492516E-4
4	0.05122671542434671
5	0.0022786658325019086
6	0.0047592387640862654
7	2.8843871297492517E-5
8	3.461264555699102E-4
9	5.7687742594985034E-5
10-11	8.653161389247754E-5
12-13	0.0
14-15	5.7687742594985034E-5
16-17	2.740167773261789E-4
18-19	1.730632277849551E-4
20-21	2.5959484167743265E-4
22-23	0.0
24-25	0.0
26-27	1.730632277849551E-4
28-29	0.0
30-31	1.730632277849551E-4
32-33	2.5959484167743265E-4
34-35	1.8748516343370137E-4
36-37	0.0
38-39	7.643625893835516E-4
40-41	5.336116190036115E-4
42-43	0.0
44-45	6.489871041935817E-4
46-47	0.0
48-49	8.653161389247754E-5
50-51	0.0
52-53	3.1728258427241767E-4
54-55	3.461264555699102E-4
56-57	1.5864129213620883E-4
58-59	7.932064606810443E-4
60-61	5.191896833548653E-4
62-63	2.019070990824476E-4
64-65	3.7497032686740274E-4
66-67	0.001067223238007223
68-69	0.002422885188989371
70-71	0.0032882013279141465
72-73	0.002653636159369311
74-75	0.002610370352423073
76-77	0.003490108426996594
78-79	0.0011537548518997006
80-81	8.94160010222268E-4
82-83	5.624554903011041E-4
84-85	5.336116190036115E-4
86-87	1.4421935648746259E-5
88-89	0.0
90-91	1.4421935648746259E-5
92-93	1.009535495412238E-4
94-95	1.1537548518997007E-4
96-97	2.451729060286864E-4
98-99	1.2979742083871632E-4
100	0.07404221762066329
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3466941.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.89986414169631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.08322985457245	34.14527285783924
2	16.268226043054973	16.56100990835808
3	6.751163039573628	10.308998445382178
4	3.4411805909137194	7.006224982582022
5	1.9487494165339028	4.959554027389281
6	1.2163869526101352	3.714835837895273
7	0.804538744357822	2.866563894918086
8	0.5647297304311454	2.299573324457767
9	0.39600490786057785	1.814093640859354
>10	1.4846340614440359	11.675903858418756
>50	0.029413629167235895	0.9907739923194196
>100	0.009451165053079524	0.8661402937948194
>500	0.001100094925101392	0.39765556830785853
>1k	9.167457709178061E-4	0.7904116019205231
>5k	9.167457709178062E-5	0.34716702982990094
>10k+	1.8334915418356123E-4	1.255820735727436
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14435	0.41636128217930446	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12478	0.35991382605011163	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7440	0.2145984024533443	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1537548518997007E-4
2	0.0	0.0	0.0	0.0	1.1537548518997007E-4
3	0.0	0.0	0.0	0.0	1.1537548518997007E-4
4	0.0	0.0	0.0	0.0	1.4421935648746258E-4
5	0.0	0.0	0.0	0.0	1.4421935648746258E-4
6	0.0	0.0	0.0	0.0	2.3075097037994014E-4
7	0.0	0.0	0.0	0.0	2.3075097037994014E-4
8	0.0	0.0	0.0	0.0	2.5959484167743265E-4
9	0.0	0.0	0.0	0.0	3.461264555699102E-4
10-11	0.0	0.0	0.0	5.7687742594985034E-5	3.461264555699102E-4
12-13	0.0	0.0	0.0	7.210967824373129E-5	0.0010239574310609843
14-15	0.0	0.0	0.0	8.653161389247754E-5	0.0012979742083871631
16-17	0.0	0.0	0.0	1.1537548518997007E-4	0.0016296787283083271
18-19	0.0	0.0	0.0	1.8748516343370134E-4	0.001788320020444536
20-21	0.0	0.0	0.0	2.5959484167743265E-4	0.0018604296986882672
22-23	0.0	0.0	0.0	6.201432328960891E-4	0.0019902271195269836
24-25	0.0	0.0	0.0	0.001398927757928387	0.002076758733419461
26-27	0.0	0.0	0.0	0.0024949948672331025	0.0021632903473119388
28-29	0.0	0.0	0.0	0.007254233631319367	0.002192134218609431
30-31	0.0	0.0	0.0	0.020118600230001032	0.0022498219612044163
32-33	0.0	0.0	0.0	0.04639536698201671	0.0024661509959356103
34-35	0.0	0.0	0.0	0.08387797773310823	0.002567104545476834
36-37	0.0	0.0	0.0	0.14332519647724032	0.00258152648112558
38-39	0.0	0.0	0.0	0.23187588136054232	0.002725745837613043
40-41	0.0	0.0	0.0	0.33183431734200264	0.002740167773261789
42-43	0.0	0.0	0.0	0.4333070565665813	0.0027834335802080275
44-45	0.0	0.0	0.0	0.559902807691276	0.003014184550587968
46-47	0.0	0.0	0.0	0.715746244311628	0.0032160916496704155
48-49	0.0	0.0	0.0	0.8835743094560882	0.003403576813104117
50-51	0.0	0.0	0.0	1.0690836677059115	0.003562218105240326
52-53	0.0	0.0	0.0	1.2484492813693686	0.003980454239053967
54-55	0.0	0.0	0.0	1.4480632926836654	0.004283314887677639
56-57	0.0	0.0	0.0	1.6879000825223156	0.004427534244165102
58-59	0.0	0.0	0.0	1.9567682288218924	0.004557331665003817
60-61	0.0	0.0	0.0	2.249144130228925	0.004643863278896295
62-63	0.0	0.0	0.0	2.5471301646033204	0.005018833605763697
64-65	0.0	0.0	0.0	2.846890097062511	0.005191896833548653
66-67	0.0	0.0	0.0	3.1936222739296687	0.005264006511792384
68-69	0.0	0.0	0.0	3.583403928708334	0.00527842844744113
70-71	0.0	0.0	0.0	4.009341376158406	0.005451491675226086
72-73	0.0	0.0	0.0	4.440485142377676	0.00589857168033722
74-75	0.0	0.0	0.0	4.871196250527483	0.0061581665220146525
76-77	0.0	0.0	0.0	5.3441059423855215	0.006201432328960891
78-79	0.0	0.0	0.0	5.877919468488215	0.006331229749799607
80-81	0.0	0.0	0.0	6.459772462236883	0.006417761363692084
82-83	0.0	0.0	0.0	7.046471226363529	0.00670620007666701
84-85	0.0	0.0	0.0	7.636876427951903	0.006720622012315756
86-87	0.0	0.0	0.0	8.237665423207375	0.006980216853993189
88	0.0	0.0	0.0	8.713329704774324	0.0072398116956706214
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1115	0.0	24.886194	1
GTACATG	11420	0.0	23.927172	1
TACATGG	11470	0.0	23.371159	2
GTATCAA	27525	0.0	23.049738	1
GAGTACT	15800	0.0	22.963644	12-13
ACATGGG	11625	0.0	22.37148	3
GGTATCA	20805	0.0	21.633438	1
AGTACTT	16520	0.0	21.493395	12-13
AGAGTAC	22335	0.0	21.452696	10-11
CATGGGG	8835	0.0	21.451668	4
TCTAACG	550	0.0	21.37697	2
GTACTTT	17250	0.0	20.760914	14-15
CAACGCA	31245	0.0	19.932121	5
ATCAACG	31575	0.0	19.77959	3
TATCAAC	32000	0.0	19.678837	2
AACGCAG	32295	0.0	19.45788	6
TCAACGC	32260	0.0	19.38875	4
TACTTTT	18550	0.0	18.976604	14-15
ATACGGG	1000	0.0	18.81146	3
CTAGTAC	1655	0.0	18.75463	3
>>END_MODULE