FastQCFastQC Report
Mon 16 Jan 2017
ERR1146522_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146522_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4020308
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT480171.1943612280452145No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT134250.33392963922167157No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC125060.31107069408612475No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC100800.250727058722864No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA97120.24157353118218805No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG77370.19244794179948402No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA76200.1895377170107365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118100.023.213661
TACATGG118350.021.9660532
ACATGGG118900.020.4806183
GTACTAG13700.020.2514631
GCTATTG21450.019.059858
CATGGGG83300.018.735794
CTATTGA25200.016.9692619
AGAGTAC90750.016.85501710-11
TAACGCC6700.016.8389644
GAGTACA96600.016.6971361
TTTAGGG27900.016.5126612
GGCTATT25350.016.4983147
GGGCTAT28200.016.497746
TGATCGC8150.016.1444239
AGTACAT93050.015.9143652
TTTCGCG900.002812974415.66588390-91
GTGATCG8150.015.5680298
CTAGGCA22350.015.5644254
ATTTAGG28900.015.4579081
CTAACGC6400.015.425333
Produced by FastQC (version 0.11.5)