##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146522_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4020308
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.150167847836535	31.0	31.0	34.0	28.0	34.0
2	31.309053186969756	31.0	31.0	34.0	28.0	34.0
3	31.425067930118786	33.0	31.0	34.0	28.0	34.0
4	34.88680966731902	37.0	35.0	37.0	32.0	37.0
5	34.95968020360629	37.0	35.0	37.0	32.0	37.0
6	35.14341388769218	37.0	35.0	37.0	32.0	37.0
7	35.10435096017519	37.0	35.0	37.0	32.0	37.0
8	35.05849999552273	37.0	35.0	37.0	32.0	37.0
9	36.67216641113069	39.0	37.0	39.0	32.0	39.0
10-11	36.65900075317613	39.0	37.0	39.0	32.0	39.0
12-13	36.61763215654124	39.0	37.0	39.0	32.0	39.0
14-15	37.951762899757924	40.0	38.0	41.0	32.5	41.0
16-17	37.99793958572329	40.0	38.0	41.0	33.0	41.0
18-19	38.0386462927716	40.0	38.0	41.0	32.0	41.0
20-21	38.137942167615016	40.0	38.0	41.0	33.0	41.0
22-23	38.15267213357782	40.0	38.0	41.0	32.0	41.0
24-25	38.17769994239247	40.0	38.0	41.0	32.0	41.0
26-27	38.225578736753505	40.0	38.0	41.0	33.0	41.0
28-29	38.22124697908717	40.0	38.0	41.0	33.0	41.0
30-31	38.20105151147624	40.0	38.0	41.0	32.5	41.0
32-33	38.15562290252389	40.0	38.0	41.0	32.0	41.0
34-35	38.0757699410095	40.0	38.0	41.0	32.0	41.0
36-37	37.9971810617495	40.0	38.0	41.0	32.0	41.0
38-39	37.93343134406618	40.0	38.0	41.0	32.0	41.0
40-41	37.91481734732761	40.0	38.0	41.0	32.0	41.0
42-43	37.759394180744366	40.0	38.0	41.0	31.0	41.0
44-45	37.63104966087175	40.0	37.0	41.0	31.0	41.0
46-47	37.508999310500585	40.0	37.0	41.0	31.0	41.0
48-49	37.39144948595978	40.0	37.0	41.0	30.5	41.0
50-51	37.25565777547392	40.0	36.0	41.0	30.0	41.0
52-53	37.165624001942135	40.0	36.0	41.0	30.0	41.0
54-55	37.04436214837271	39.5	36.0	41.0	30.0	41.0
56-57	36.80271984136539	39.0	35.0	41.0	30.0	41.0
58-59	36.614378176995395	39.0	35.0	41.0	30.0	41.0
60-61	36.37116260744202	39.0	35.0	41.0	29.5	41.0
62-63	36.17419759879094	38.0	35.0	40.0	29.0	41.0
64-65	35.89442288998754	37.5	35.0	40.0	29.0	41.0
66-67	35.60520848153922	37.0	34.5	40.0	29.0	41.0
68-69	35.28825552669099	36.5	34.0	39.5	28.5	41.0
70-71	34.949884063609055	36.0	34.0	39.0	28.0	41.0
72-73	34.60249401289653	35.5	34.0	39.0	28.0	41.0
74-75	34.29114423571528	35.0	34.0	37.5	28.0	40.0
76-77	34.00848430020784	35.0	34.0	37.0	28.0	39.0
78-79	33.65783629015488	35.0	33.5	37.0	27.0	39.0
80-81	33.34763965845403	35.0	33.0	36.0	26.5	39.0
82-83	33.077625893339516	35.0	33.0	36.0	26.0	37.0
84-85	32.87496766416901	35.0	33.0	35.5	26.0	37.0
86-87	32.717858681474155	35.0	33.0	35.0	26.0	37.0
88-89	32.511206977176876	35.0	33.0	35.0	26.0	36.0
90-91	32.304786225333984	35.0	33.0	35.0	25.0	36.0
92-93	32.11573789868836	35.0	33.0	35.0	25.0	36.0
94-95	31.986575406660386	35.0	32.0	35.0	25.0	35.5
96-97	31.902351511376743	35.0	32.0	35.0	25.0	35.0
98-99	31.815201223388854	35.0	32.0	35.0	24.5	35.0
100	30.631513804414986	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36235618653693535
1101	2	0.33509237790922697
1101	3	0.31051674408182706
1101	4	0.385005302035907
1101	5	0.25946403467383305
1101	6	0.35720273592980334
1101	7	0.44447837769728693
1101	8	0.46940788461406413
1101	9	0.5556842645099991
1101	10-11	0.5782460114391057
1101	12-13	0.5408956027691829
1101	14-15	0.5752185273937087
1101	16-17	0.5548443818778139
1101	18-19	0.5747862503926768
1101	20-21	0.602640470557013
1101	22-23	0.5928837890670877
1101	24-25	0.6106740101740371
1101	26-27	0.5077433975819474
1101	28-29	0.5704276122148855
1101	30-31	0.5832564981105364
1101	32-33	0.622979818390327
1101	34-35	0.5669259551887151
1101	36-37	0.6278934917101182
1101	38-39	0.6205660333491494
1101	40-41	0.623042996022015
1101	42-43	0.6573651781228733
1101	44-45	0.6121563999555804
1101	46-47	0.5408243127657997
1101	48-49	0.5443082752743038
1101	50-51	0.4809643724860351
1101	52-53	0.5573062524901289
1101	54-55	0.5063218497352935
1101	56-57	0.5433454502878803
1101	58-59	0.5245346720464283
1101	60-61	0.5509724006871934
1101	62-63	0.5930567224927401
1101	64-65	0.6215092504658841
1101	66-67	0.6006530465669968
1101	68-69	0.5293910217534616
1101	70-71	0.4662459226087563
1101	72-73	0.5340699033995833
1101	74-75	0.49055896935011845
1101	76-77	0.4277251086640277
1101	78-79	0.4658137089942329
1101	80-81	0.48107768105919035
1101	82-83	0.44939417768576817
1101	84-85	0.3933391807843023
1101	86-87	0.43525629571914237
1101	88-89	0.4148642560092952
1101	90-91	0.4850781365126835
1101	92-93	0.5417348394276544
1101	94-95	0.5395277288431046
1101	96-97	0.4506108309054717
1101	98-99	0.46207962332004726
1101	100	0.4214692625744014
1102	1	0.31657505022276666
1102	2	0.3437799990319377
1102	3	0.29354112420433864
1102	4	0.3876462073074407
1102	5	0.2867645331255062
1102	6	0.3738547448153611
1102	7	0.45154547548712287
1102	8	0.428008074504568
1102	9	0.505641808490239
1102	10-11	0.4846198339343175
1102	12-13	0.5122737804911921
1102	14-15	0.5931268838099655
1102	16-17	0.5929923098890129
1102	18-19	0.5916284510664838
1102	20-21	0.5704379461952627
1102	22-23	0.582212521497901
1102	24-25	0.5676482136408794
1102	26-27	0.5789195571039016
1102	28-29	0.6477723816666483
1102	30-31	0.7040419504479232
1102	32-33	0.6619212194999662
1102	34-35	0.5882523007025995
1102	36-37	0.6233397423745828
1102	38-39	0.632120651842456
1102	40-41	0.6287723444135906
1102	42-43	0.6584856925260567
1102	44-45	0.62517744995219
1102	46-47	0.6684942395727305
1102	48-49	0.6210373852500624
1102	50-51	0.6393128391253171
1102	52-53	0.5957195669244371
1102	54-55	0.5685960571079747
1102	56-57	0.6703809688720455
1102	58-59	0.6704552787086868
1102	60-61	0.6698917084043288
1102	62-63	0.624139295095226
1102	64-65	0.6755997478532549
1102	66-67	0.6611464678441124
1102	68-69	0.6798104564466172
1102	70-71	0.6584740643288072
1102	72-73	0.6058961707241153
1102	74-75	0.617458654806768
1102	76-77	0.5380156369205906
1102	78-79	0.5628955182885846
1102	80-81	0.5266361737369039
1102	82-83	0.5108077032385907
1102	84-85	0.5352563219957247
1102	86-87	0.5217698557275554
1102	88-89	0.5196320713842653
1102	90-91	0.5666136410851976
1102	92-93	0.5615076525324554
1102	94-95	0.5633699456339372
1102	96-97	0.595120181466303
1102	98-99	0.6416597297998656
1102	100	0.6796135148874463
1103	1	0.22910412698541904
1103	2	0.25624089579940446
1103	3	0.23792362734596395
1103	4	0.302613721545022
1103	5	0.2951787466492064
1103	6	0.37961795970266365
1103	7	0.4011470904172043
1103	8	0.3750135021669294
1103	9	0.5291465276153957
1103	10-11	0.49831331806575463
1103	12-13	0.468557034949626
1103	14-15	0.5382065608239444
1103	16-17	0.5649201995176298
1103	18-19	0.5868210929744606
1103	20-21	0.566123890421359
1103	22-23	0.5402163181878308
1103	24-25	0.5665606758745128
1103	26-27	0.5054549438582683
1103	28-29	0.5135466925139482
1103	30-31	0.4965989676399758
1103	32-33	0.49862077298783447
1103	34-35	0.4896045372738129
1103	36-37	0.5083541804912102
1103	38-39	0.4699699072099577
1103	40-41	0.38084648681620337
1103	42-43	0.42743973118811596
1103	44-45	0.3521205798150291
1103	46-47	0.37412844721423255
1103	48-49	0.4023908249976671
1103	50-51	0.3909906017519873
1103	52-53	0.4151789396684791
1103	54-55	0.4079679990346605
1103	56-57	0.39270122768865434
1103	58-59	0.46183585676809713
1103	60-61	0.3971730645178013
1103	62-63	0.4436782111669828
1103	64-65	0.3904997505269847
1103	66-67	0.383129168841414
1103	68-69	0.3963922323349678
1103	70-71	0.3883843608449453
1103	72-73	0.46434246895143616
1103	74-75	0.45229194803421535
1103	76-77	0.3648745444364323
1103	78-79	0.36891674766747684
1103	80-81	0.26310779214283286
1103	82-83	0.3296862091608901
1103	84-85	0.34160174056741255
1103	86-87	0.2995866253326369
1103	88-89	0.25473607929849607
1103	90-91	0.2642801473283427
1103	92-93	0.31438123880019475
1103	94-95	0.3500928942182
1103	96-97	0.3753493197246378
1103	98-99	0.3421764272143655
1103	100	0.381390205350268
1104	1	0.13853448099145993
1104	2	0.11263620864574264
1104	3	0.11972226664960317
1104	4	0.1571823584323937
1104	5	0.09835084258888571
1104	6	0.1777344382934345
1104	7	0.21701911892779435
1104	8	0.19095003096008156
1104	9	0.27338733133284876
1104	10-11	0.3084938789186822
1104	12-13	0.27462182545017555
1104	14-15	0.3457087790612192
1104	16-17	0.32505874199762275
1104	18-19	0.33633614270756595
1104	20-21	0.362442215623922
1104	22-23	0.32785649314401155
1104	24-25	0.4010521021295119
1104	26-27	0.37849945038450983
1104	28-29	0.37824641674028925
1104	30-31	0.4345794664554745
1104	32-33	0.37096140104352315
1104	34-35	0.38501787824866796
1104	36-37	0.4354804599298632
1104	38-39	0.3837807556382913
1104	40-41	0.3966898762694413
1104	42-43	0.3791085511923953
1104	44-45	0.33214073532128197
1104	46-47	0.36873706562350606
1104	48-49	0.3501282229803806
1104	50-51	0.3370762866165222
1104	52-53	0.2530746196139475
1104	54-55	0.22777780640579692
1104	56-57	0.31706782359804464
1104	58-59	0.3326878029925666
1104	60-61	0.30219792941001344
1104	62-63	0.33351257723703753
1104	64-65	0.41304388063517194
1104	66-67	0.34467120818192853
1104	68-69	0.38944721932507775
1104	70-71	0.36380594241317255
1104	72-73	0.348763986618458
1104	74-75	0.3429896627006741
1104	76-77	0.2185829445831331
1104	78-79	0.2572660263492992
1104	80-81	0.22837633697252357
1104	82-83	0.17328126121042686
1104	84-85	0.18254639013917284
1104	86-87	0.22069900226457406
1104	88-89	0.24143938598635373
1104	90-91	0.2585143445927116
1104	92-93	0.22614879615652228
1104	94-95	0.2585302667069911
1104	96-97	0.23490434765146873
1104	98-99	0.20990826379501115
1104	100	0.3199046952821689
1105	1	0.1602522714153629
1105	2	0.1093209850706387
1105	3	0.08473349351633885
1105	4	0.22439443556045546
1105	5	0.1684182267755503
1105	6	0.230935662847493
1105	7	0.3162076213596734
1105	8	0.2987003681236473
1105	9	0.3354812329662664
1105	10-11	0.3102823048063499
1105	12-13	0.34260994385847
1105	14-15	0.39611342322834986
1105	16-17	0.40702350497301154
1105	18-19	0.3741495586058008
1105	20-21	0.34992661849674533
1105	22-23	0.3489342282936576
1105	24-25	0.42433889554661164
1105	26-27	0.3770328798599536
1105	28-29	0.3428243368653838
1105	30-31	0.4138101939868264
1105	32-33	0.40346801054764825
1105	34-35	0.4001118619940698
1105	36-37	0.33256037953928796
1105	38-39	0.35808972835859976
1105	40-41	0.3487622557509411
1105	42-43	0.42024185176606466
1105	44-45	0.41926423494182075
1105	46-47	0.3710145079794245
1105	48-49	0.3513932807288569
1105	50-51	0.3392178547051756
1105	52-53	0.28877371570528254
1105	54-55	0.2282477953685671
1105	56-57	0.312801799405527
1105	58-59	0.3131947461803932
1105	60-61	0.3054414007727857
1105	62-63	0.29591462549053205
1105	64-65	0.3060993710984974
1105	66-67	0.25192452200078463
1105	68-69	0.27593687953597623
1105	70-71	0.24565785567993004
1105	72-73	0.25581607498934034
1105	74-75	0.24755012157770295
1105	76-77	0.26985583042614536
1105	78-79	0.2535404982434386
1105	80-81	0.23934531077318155
1105	82-83	0.23427661173091252
1105	84-85	0.19888070545354708
1105	86-87	0.2944692315996491
1105	88-89	0.3117343340304046
1105	90-91	0.23994498378024787
1105	92-93	0.2869150823284201
1105	94-95	0.2489303474653468
1105	96-97	0.25310294347264417
1105	98-99	0.2056926018338423
1105	100	0.41622773839581484
1106	1	0.14512535475363464
1106	2	0.18075911519714083
1106	3	0.17809346420255068
1106	4	0.14519981684196637
1106	5	0.17574643866268502
1106	6	0.2325998506363618
1106	7	0.2478548003779224
1106	8	0.31179138292070974
1106	9	0.3692880460701673
1106	10-11	0.34998391988696653
1106	12-13	0.3123144823215327
1106	14-15	0.3647153432062211
1106	16-17	0.3652061418216448
1106	18-19	0.3487815722542109
1106	20-21	0.36376059107009695
1106	22-23	0.4101304067766165
1106	24-25	0.4381262421689911
1106	26-27	0.41532225191834726
1106	28-29	0.35613554609678033
1106	30-31	0.40246915167281117
1106	32-33	0.38707219773444024
1106	34-35	0.4312905119417181
1106	36-37	0.4615354035519488
1106	38-39	0.4261970134370756
1106	40-41	0.39812126357892197
1106	42-43	0.4373798579957153
1106	44-45	0.43471947355850205
1106	46-47	0.4739401482307528
1106	48-49	0.4840987923983988
1106	50-51	0.44783703510444894
1106	52-53	0.46634679791464606
1106	54-55	0.36654571649791734
1106	56-57	0.3853338460742819
1106	58-59	0.3872172357639343
1106	60-61	0.4305100755656568
1106	62-63	0.3858807208393742
1106	64-65	0.41153793255914195
1106	66-67	0.3786763759850018
1106	68-69	0.3763753516253132
1106	70-71	0.33566340375652004
1106	72-73	0.3224681953000541
1106	74-75	0.30455337469324206
1106	76-77	0.2765191080388263
1106	78-79	0.2564724650424637
1106	80-81	0.3082841017769766
1106	82-83	0.2975816002490532
1106	84-85	0.27267953158496283
1106	86-87	0.2662159631969203
1106	88-89	0.23590066437873958
1106	90-91	0.29762175551935144
1106	92-93	0.2852618307651227
1106	94-95	0.2502050645110856
1106	96-97	0.26207552858940275
1106	98-99	0.26847153438297156
1106	100	0.47338198198060155
1107	1	0.04497687914326676
1107	2	0.06379435745653694
1107	3	0.04752536470148172
1107	4	-0.009566754566336044
1107	5	0.052136475745165
1107	6	0.08130507748049354
1107	7	0.045717458853658854
1107	8	0.12186236964214459
1107	9	0.1397534153281157
1107	10-11	0.11502824460789896
1107	12-13	0.1456743380928387
1107	14-15	0.1971866556031756
1107	16-17	0.22010398261718223
1107	18-19	0.16616270168971425
1107	20-21	0.18887130139770392
1107	22-23	0.2009557227717238
1107	24-25	0.2831551505143395
1107	26-27	0.21890085719618924
1107	28-29	0.19484816641801928
1107	30-31	0.1996537782001866
1107	32-33	0.2498877925053975
1107	34-35	0.2095704784600585
1107	36-37	0.21248890465832915
1107	38-39	0.21130805070793457
1107	40-41	0.19591879751485664
1107	42-43	0.19582911718009655
1107	44-45	0.13815844403863764
1107	46-47	0.1626823519128422
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2216	64-65	-0.5673125827707821
2216	66-67	-0.5739683452949365
2216	68-69	-0.6249344506907448
2216	70-71	-0.5866398220918612
2216	72-73	-0.52401095755733
2216	74-75	-0.48090268904390854
2216	76-77	-0.6046901627010897
2216	78-79	-0.5143180757615227
2216	80-81	-0.49975095105571654
2216	82-83	-0.4924705176074866
2216	84-85	-0.47940167349425167
2216	86-87	-0.49270239632531343
2216	88-89	-0.5272460324408996
2216	90-91	-0.5179345473620813
2216	92-93	-0.5449194120053065
2216	94-95	-0.5350507808769365
2216	96-97	-0.4592169877542851
2216	98-99	-0.5090613479554449
2216	100	-0.6355723466608261
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1287.0
1	275.0
2	234.0
3	239.0
4	171.0
5	211.0
6	352.0
7	902.0
8	2417.0
9	3213.0
10	3161.0
11	3496.0
12	4355.0
13	5984.0
14	8936.0
15	10292.0
16	10857.0
17	11782.0
18	13227.0
19	14705.0
20	16439.0
21	18654.0
22	20691.0
23	23690.0
24	29122.0
25	35859.0
26	39155.0
27	37255.0
28	40085.0
29	49742.0
30	59101.0
31	67562.0
32	86830.0
33	117917.0
34	173695.0
35	267083.0
36	474127.0
37	922077.0
38	1108774.0
39	335812.0
40	542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50395259377295	19.728982782874997	13.40739726368469	23.35966735966736
2	14.73448150125507	21.85593516696721	39.15197855492128	24.25760477685644
3	19.766149418920886	25.84965005806435	30.545033164513164	23.839167358501605
4	11.826784292658301	17.630431070149907	40.74382391351213	29.798960723679656
5	12.564109202598772	39.38462135926197	35.45093894060554	12.600330497533715
6	28.97717158494325	38.96052324630404	18.13750228114854	13.924802887604171
7	25.641111819819983	33.627032579529335	23.28534361840857	17.446511982242114
8	24.64852446249824	33.90569426470596	21.100831093068383	20.344950179727427
9	24.91051194634401	17.393017591932484	21.798767130925874	35.89770333079763
10-11	24.175950972294206	26.188363736664776	28.97364297780878	20.66204231323223
12-13	25.906596459951643	24.610351860400367	27.812754324995094	21.670297354652895
14-15	22.367183227164222	26.174820183822877	26.974853741045283	24.48314284796761
16-17	21.473914705987788	28.900210008722983	27.41594672211136	22.209928563177872
18-19	21.318443319782624	29.84050325988733	27.51761882594268	21.323434594387365
20-21	23.00041494798464	27.57891914886626	26.57083450843848	22.84983139471062
22-23	22.49964122739686	27.590640024186193	26.952877381170342	22.956841367246604
24-25	23.321567744945213	27.698264561191433	26.77999851633599	22.20016917752737
26-27	22.68363155264676	27.118257394393147	28.090846690608846	22.107264362351245
28-29	21.735342138441656	29.51903665182663	26.807314059415997	21.93830715031572
30-31	22.4771394205006	27.65219878686848	26.642089855480535	23.228571937150384
32-33	22.614083556089323	28.101947182370125	26.679445727827655	22.604523533712896
34-35	23.606352777211384	27.606801411230343	26.740454763524806	22.046391048033467
36-37	22.940838169501145	27.029036924172374	27.930020589502092	22.100104316824385
38-39	22.418565128975267	28.106321192336576	27.445884363429222	22.029229315258934
40-41	22.401883222714787	28.194640343772043	27.1559475658256	22.24752886768757
42-43	22.03344234965358	28.185739709261043	26.154020242378735	23.626797698706643
44-45	22.477484486546707	27.48767326208867	26.74964989699192	23.285192354372708
46-47	23.337326984154647	28.06083460870832	26.72180601811022	21.880032389026816
48-49	22.72012311435396	27.68228041405443	27.046043518514022	22.551552953077593
50-51	21.325572504877595	27.999904894249	28.14425650270012	22.530266098173286
52-53	22.293725749137526	28.60909601548868	26.320252806526916	22.776925428846877
54-55	21.65389974268643	27.55316591789592	27.01780033308483	23.775134006332816
56-57	22.91746106320975	27.082682099340616	26.749399682079545	23.250457155370082
58-59	23.6336052990679	27.01363347707011	26.70603337164848	22.64672785221351
60-61	22.35049130461359	27.556058174489856	27.99401139831185	22.0994391225847
62-63	21.822675148197725	27.68801341348759	27.54581630254645	22.943495135768238
64-65	21.401224990930466	28.726956099968305	26.742701359222526	23.129117549878703
66-67	22.281527791247825	27.327104837602246	26.266128318399527	24.125239052750402
68-69	21.753289316017728	27.37202682371308	26.937837731848767	23.936846128420424
70-71	22.80769616713009	27.12887210545279	26.612354832227748	23.451076895189374
72-73	22.405990700222223	26.452327745581982	28.16642493884182	22.97525661535397
74-75	21.54869998377773	28.587782453282546	26.99481298264516	22.868704580294562
76-77	21.93513094398338	26.978676021486077	26.968579370013767	24.11761366451678
78-79	22.103676187341055	27.193173581093482	27.29301354332387	23.410136688241597
80-81	22.65476214931193	26.87715576404853	27.162966269081686	23.305115817557855
82-83	22.29084035129461	26.28382282578287	28.42554760765553	22.99978921526699
84-85	22.140484366514602	27.225042958438937	27.587248396512926	23.04722427853353
86-87	21.722599083316236	27.65380397867047	27.3382208526633	23.28537608534999
88-89	21.74626012240946	27.241502043747257	26.608377988108135	24.403859845735145
90-91	22.18984425001615	26.23657758622943	27.183362500066917	24.3902156636875
92-93	22.919379948796305	26.837411213275615	27.271121399763526	22.972087438164554
94-95	22.006195923228912	26.571338514611682	27.67265478106377	23.749810781095636
96-97	21.055348607695407	26.648256301757367	28.60319157252766	23.69320351801957
98-99	21.799560709249633	27.390246410686803	26.875594961079617	23.93459791898395
100	21.88537092777639	25.62049458034281	26.95291061112747	25.54122388075333
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2271.0
1	1578.0
2	936.5
3	974.0
4	899.0
5	821.0
6	793.5
7	805.0
8	823.0
9	829.5
10	772.0
11	680.0
12	656.0
13	705.0
14	765.5
15	840.5
16	983.5
17	1199.5
18	1496.5
19	1888.5
20	2521.5
21	3453.5
22	4760.5
23	6693.5
24	9062.5
25	12266.0
26	17243.5
27	23283.0
28	29291.5
29	37063.5
30	46577.5
31	55654.5
32	65389.0
33	73486.5
34	80783.5
35	88628.0
36	96778.0
37	107558.5
38	113536.5
39	114597.0
40	116974.5
41	118756.5
42	121070.5
43	125565.0
44	130743.5
45	141514.5
46	157238.0
47	194711.0
48	260600.0
49	250571.0
50	179196.0
51	147034.0
52	135291.0
53	129010.0
54	123484.0
55	112670.0
56	101452.5
57	88574.0
58	76406.0
59	68033.0
60	56233.5
61	44171.5
62	35228.0
63	27144.0
64	19098.5
65	12927.5
66	9721.5
67	7250.5
68	5029.0
69	3614.5
70	2796.5
71	2143.5
72	1737.0
73	1520.5
74	1202.0
75	854.0
76	656.0
77	501.0
78	351.0
79	251.0
80	175.5
81	121.0
82	93.0
83	71.5
84	58.5
85	45.0
86	31.0
87	23.0
88	17.0
89	15.0
90	14.5
91	13.5
92	16.5
93	15.5
94	12.0
95	10.5
96	9.0
97	5.5
98	4.5
99	6.0
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09845016849455315
2	0.06474628312059673
3	0.0799192499679129
4	0.08163553638178965
5	0.082928969621233
6	0.09180888628433444
7	0.08989361014131256
8	0.09128653824532847
9	0.0749196330231415
10-11	0.08243149529837017
12-13	0.07296704630590492
14-15	0.08180965239479164
16-17	0.08287922218894672
18-19	0.08203351584007991
20-21	0.07305410431240592
22-23	0.07809103183139203
24-25	0.08024260827777374
26-27	0.07438484812606397
28-29	0.0835632493828831
30-31	0.08739380166892685
32-33	0.0890727775085889
34-35	0.0913114119614716
36-37	0.09198300229733643
38-39	0.0926297189170581
40-41	0.08896084578594476
42-43	0.09412214188564656
44-45	0.09036621074803225
46-47	0.08744354910121313
48-49	0.09649758177731657
50-51	0.09269190320741595
52-53	0.09840042106226687
54-55	0.089881173283241
56-57	0.09687068751946369
58-59	0.10085048210236629
60-61	0.0997809123082112
62-63	0.10078829781200843
64-65	0.10235534192902633
66-67	0.10438254979469234
68-69	0.10162156730280367
70-71	0.10394725976218737
72-73	0.10155938301244581
74-75	0.10505414013055715
76-77	0.10260407909045775
78-79	0.10897175042310192
80-81	0.1041089389171178
82-83	0.10851158667445379
84-85	0.10489246097562675
86-87	0.10178324645773408
88-89	0.10032813406336032
90-91	0.10027838663107402
92-93	0.09454499506007998
94-95	0.09442062647936426
96-97	0.09277896121391695
98-99	0.10668336853793291
100	0.24871228771526954
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4020308.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.92279318623005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.27055702156221	36.659913631470936
2	15.603902251849771	16.516041835455898
3	6.264349355363821	9.945805961406393
4	3.1535654787939604	6.675819745337893
5	1.8369461187541816	4.860815976853777
6	1.1086330435865381	3.5203174371090675
7	0.6970732164845714	2.58237431701712
8	0.48604195472332556	2.0578158279722842
9	0.3476383213216811	1.6558191884623967
>10	1.2054116175461058	9.646082905790207
>50	0.020178693925906672	0.6948376147937643
>100	0.004716508390339682	0.4053623052066089
>500	1.5175656887424701E-4	0.0542118251867157
>1k	3.0351313774202345E-4	0.34378328549327714
>5k	2.2763485330651762E-4	1.0066935152471634
>10k+	3.0351313774202345E-4	3.374304627196483
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	48017	1.1943612280452145	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	13425	0.33392963922167157	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	12506	0.31107069408612475	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	10080	0.250727058722864	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	9712	0.24157353118218805	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	7737	0.19244794179948402	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	7620	0.1895377170107365	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.4873716143141273E-5	0.0
9	0.0	0.0	0.0	2.4873716143141273E-5	0.0
10-11	0.0	0.0	0.0	2.4873716143141273E-5	0.0
12-13	0.0	0.0	0.0	2.4873716143141273E-5	0.0
14-15	0.0	0.0	0.0	7.462114842942382E-5	0.0
16-17	0.0	0.0	0.0	1.36805438787277E-4	0.0
18-19	0.0	0.0	0.0	2.487371614314127E-4	0.0
20-21	0.0	0.0	0.0	3.9797945829026036E-4	0.0
22-23	0.0	0.0	0.0	7.462114842942382E-4	0.0
24-25	0.0	0.0	0.0	0.0012063752329423516	0.0
26-27	0.0	0.0	0.0	0.0025868664788866926	0.0
28-29	0.0	0.0	0.0	0.008357568624095467	0.0
30-31	0.0	0.0	0.0	0.02282163456133212	1.2436858071570636E-5
32-33	0.0	0.0	0.0	0.056139977335069854	2.4873716143141273E-5
34-35	0.0	0.0	0.0	0.10607396249242595	2.4873716143141273E-5
36-37	0.0	0.0	0.0	0.17599397857079607	2.4873716143141273E-5
38-39	0.0	0.0	0.0	0.28379666433517037	2.4873716143141273E-5
40-41	0.0	0.0	0.0	0.407605586437656	2.4873716143141273E-5
42-43	0.0	0.0	0.0	0.5341506173158872	2.4873716143141273E-5
44-45	0.0	0.0	0.0	0.6945239021487906	2.4873716143141273E-5
46-47	0.0	0.0	0.0	0.8938369896037817	2.4873716143141273E-5
48-49	0.0	0.0	0.0	1.0956498855311585	2.4873716143141273E-5
50-51	0.0	1.2436858071570636E-5	0.0	1.3114666836471236	4.9747432286282545E-5
52-53	0.0	2.4873716143141273E-5	0.0	1.5283157409830292	4.9747432286282545E-5
54-55	0.0	2.4873716143141273E-5	0.0	1.7722522751988157	4.9747432286282545E-5
56-57	0.0	2.4873716143141273E-5	0.0	2.07516439038999	4.9747432286282545E-5
58-59	0.0	2.4873716143141273E-5	0.0	2.389879083891085	4.9747432286282545E-5
60-61	0.0	2.4873716143141273E-5	0.0	2.730275889309973	4.9747432286282545E-5
62-63	0.0	2.4873716143141273E-5	0.0	3.0694538826378475	4.9747432286282545E-5
64-65	0.0	2.4873716143141273E-5	0.0	3.4169894445898175	4.9747432286282545E-5
66-67	0.0	2.4873716143141273E-5	0.0	3.8267217337577124	4.9747432286282545E-5
68-69	0.0	2.4873716143141273E-5	0.0	4.291698546479523	4.9747432286282545E-5
70-71	0.0	2.4873716143141273E-5	0.0	4.783737962365072	4.9747432286282545E-5
72-73	0.0	2.4873716143141273E-5	0.0	5.273837228391457	4.9747432286282545E-5
74-75	0.0	2.4873716143141273E-5	0.0	5.776236547050624	4.9747432286282545E-5
76-77	0.0	2.4873716143141273E-5	0.0	6.301494810845338	4.9747432286282545E-5
78-79	0.0	2.4873716143141273E-5	0.0	6.940326462549636	4.9747432286282545E-5
80-81	0.0	2.4873716143141273E-5	0.0	7.616170203874927	4.9747432286282545E-5
82-83	0.0	2.4873716143141273E-5	0.0	8.27912936023807	4.9747432286282545E-5
84-85	0.0	2.4873716143141273E-5	0.0	8.944911683383463	4.9747432286282545E-5
86-87	0.0	2.4873716143141273E-5	0.0	9.622757758858278	6.218429035785317E-5
88	0.0	4.9747432286282545E-5	0.0	10.163400416087523	7.462114842942381E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	11810	0.0	23.21366	1
TACATGG	11835	0.0	21.966053	2
ACATGGG	11890	0.0	20.480618	3
GTACTAG	1370	0.0	20.251463	1
GCTATTG	2145	0.0	19.05985	8
CATGGGG	8330	0.0	18.73579	4
CTATTGA	2520	0.0	16.969261	9
AGAGTAC	9075	0.0	16.855017	10-11
TAACGCC	670	0.0	16.838964	4
GAGTACA	9660	0.0	16.697136	1
TTTAGGG	2790	0.0	16.512661	2
GGCTATT	2535	0.0	16.498314	7
GGGCTAT	2820	0.0	16.49774	6
TGATCGC	815	0.0	16.144423	9
AGTACAT	9305	0.0	15.914365	2
TTTCGCG	90	0.0028129744	15.665883	90-91
GTGATCG	815	0.0	15.568029	8
CTAGGCA	2235	0.0	15.564425	4
ATTTAGG	2890	0.0	15.457908	1
CTAACGC	640	0.0	15.42533	3
>>END_MODULE