FastQCFastQC Report
Mon 16 Jan 2017
ERR1146522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4020308
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT495631.232815993202511No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149830.37268288897268564No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC140600.34972444897256627No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT135700.33753632806242706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131010.32587055519129376No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC112420.27963031688119416No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG83220.20699906574322166No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC77580.19297028983849No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75830.18861738951344026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG114000.023.1855721
GTACTAG13350.022.8991031
GAGTACT179600.022.51750212-13
TACATGG114400.022.2000562
AGAGTAC236150.021.68264610-11
AGTACTT186300.021.1022512-13
ACATGGG114900.020.7107223
GTACTTT197550.020.18638814-15
CTAACGC5250.019.7073463
CATGGGG81800.019.6624454
TATTCGC2001.4095704E-518.7989239
TAGTACT18100.018.4477984
TAACGCC6000.018.0277424
ACTTTTT222750.017.94509516-17
TACTTTT219450.017.81851614-15
GTACCGT3351.6636477E-816.8361136
ATGGGGG55450.016.6984235
TGATCGC7900.016.6572749
CTGTGCG17050.016.5386419
TCTAACG6000.016.4609322
Produced by FastQC (version 0.11.5)