##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146522_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4020308
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30417072522802	33.0	31.0	34.0	30.0	34.0
2	32.348189740686536	34.0	31.0	34.0	30.0	34.0
3	32.466158065501446	34.0	31.0	34.0	30.0	34.0
4	35.97889340816674	37.0	35.0	37.0	35.0	37.0
5	36.03363349275727	37.0	35.0	37.0	35.0	37.0
6	36.12747058185592	37.0	36.0	37.0	35.0	37.0
7	36.10693658296827	37.0	36.0	37.0	35.0	37.0
8	36.11351568088813	37.0	36.0	37.0	35.0	37.0
9	37.88784217527612	39.0	38.0	39.0	35.0	39.0
10-11	37.8408120721099	39.0	38.0	39.0	35.0	39.0
12-13	37.82588610126388	39.0	38.0	39.0	35.0	39.0
14-15	39.13369013020893	40.0	39.0	41.0	36.0	41.0
16-17	39.1151720216461	40.0	39.0	41.0	36.0	41.0
18-19	39.24095206138435	40.5	39.0	41.0	36.0	41.0
20-21	39.29868184228671	41.0	39.0	41.0	36.0	41.0
22-23	39.2573514268061	40.5	39.0	41.0	36.0	41.0
24-25	39.231925389796004	41.0	39.0	41.0	36.0	41.0
26-27	39.13724284308566	40.5	39.0	41.0	35.5	41.0
28-29	39.091226716958005	40.0	39.0	41.0	35.0	41.0
30-31	38.95428944747517	40.0	38.5	41.0	35.0	41.0
32-33	38.82509238098176	40.0	38.0	41.0	35.0	41.0
34-35	38.723655625389895	40.0	38.0	41.0	35.0	41.0
36-37	38.62578053721257	40.0	38.0	41.0	35.0	41.0
38-39	38.53331685035077	40.0	38.0	41.0	35.0	41.0
40-41	38.46986636347265	40.0	38.0	41.0	34.5	41.0
42-43	38.35445431046577	40.0	38.0	41.0	34.0	41.0
44-45	38.19727294525693	40.0	38.0	41.0	34.0	41.0
46-47	37.93403577536846	40.0	38.0	41.0	33.0	41.0
48-49	37.61532113957438	40.0	37.0	41.0	33.0	41.0
50-51	37.49105926710093	40.0	37.0	41.0	32.5	41.0
52-53	37.436250531053844	40.0	37.0	41.0	32.0	41.0
54-55	37.288900253413416	40.0	36.5	41.0	32.0	41.0
56-57	37.127255051105536	40.0	36.0	41.0	31.5	41.0
58-59	36.950983108756844	39.0	36.0	41.0	31.0	41.0
60-61	36.706101124590454	39.0	35.0	41.0	31.0	41.0
62-63	36.51436195933247	39.0	35.0	41.0	31.0	41.0
64-65	36.253139436083	38.5	35.0	40.5	31.0	41.0
66-67	35.96025764692656	37.5	35.0	40.0	31.0	41.0
68-69	35.62078191521644	37.0	35.0	39.5	30.0	41.0
70-71	35.24982526214409	36.0	35.0	39.0	30.0	41.0
72-73	34.905783959835915	36.0	35.0	39.0	29.5	41.0
74-75	34.60804296586232	35.0	34.0	38.5	29.0	40.0
76-77	34.315727302485286	35.0	34.0	37.0	29.0	39.5
78-79	34.02093720182633	35.0	34.0	37.0	29.0	39.0
80-81	33.74654205598178	35.0	34.0	36.0	29.0	39.0
82-83	33.47047129224925	35.0	34.0	36.0	29.0	37.5
84-85	33.26745040429738	35.0	34.0	36.0	29.0	37.0
86-87	33.125195631777466	35.0	34.0	35.0	29.0	37.0
88-89	32.927003975814785	35.0	34.0	35.0	29.0	36.0
90-91	32.832995631180495	35.0	34.0	35.0	28.5	36.0
92-93	32.72619212259359	35.0	34.0	35.0	28.0	36.0
94-95	32.64225029525102	35.0	34.0	35.0	27.0	36.0
96-97	32.58677394866264	35.0	34.0	35.0	27.0	35.0
98-99	32.54247435768603	35.0	34.0	35.0	27.0	35.0
100	31.79254101924529	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33829436986371064
1101	2	0.3049221556261301
1101	3	0.2621774577812488
1101	4	0.25788337193866084
1101	5	0.22284800569518382
1101	6	0.25052101956284645
1101	7	0.23274633661204547
1101	8	0.23711821834544367
1101	9	0.28691923859395985
1101	10-11	0.30397051988943247
1101	12-13	0.32584391753045594
1101	14-15	0.49164326859779095
1101	16-17	0.521006416590545
1101	18-19	0.45808165177953697
1101	20-21	0.4521958671148951
1101	22-23	0.4305239315574241
1101	24-25	0.4303306120715078
1101	26-27	0.4460805525845899
1101	28-29	0.456633141428334
1101	30-31	0.5338198799615199
1101	32-33	0.5490642487743358
1101	34-35	0.5260092450375566
1101	36-37	0.5477091715507214
1101	38-39	0.5385991246103217
1101	40-41	0.5850273966134196
1101	42-43	0.5604934527016923
1101	44-45	0.6225009013275624
1101	46-47	0.7066205873200175
1101	48-49	0.7278749439069543
1101	50-51	0.6833788198271691
1101	52-53	0.6676920530835844
1101	54-55	0.7188610677905771
1101	56-57	0.6519814734148852
1101	58-59	0.6172816860013413
1101	60-61	0.6602968996439316
1101	62-63	0.6648545749152817
1101	64-65	0.5907549111086681
1101	66-67	0.6125869252174354
1101	68-69	0.5875679090956893
1101	70-71	0.5567862550253864
1101	72-73	0.6122346893881314
1101	74-75	0.6182158766874508
1101	76-77	0.5167276539359023
1101	78-79	0.4806610114725913
1101	80-81	0.5128239124682707
1101	82-83	0.48624348266655204
1101	84-85	0.44753468916799477
1101	86-87	0.41962616095243277
1101	88-89	0.5094726831287915
1101	90-91	0.5265716912861222
1101	92-93	0.488817888330658
1101	94-95	0.5104904913367818
1101	96-97	0.4441948623637231
1101	98-99	0.4306472466241189
1101	100	0.5413540061151849
1102	1	0.2790533031605733
1102	2	0.2495309888885302
1102	3	0.25978001397049866
1102	4	0.21006550100350552
1102	5	0.21244290499063823
1102	6	0.24072989040260495
1102	7	0.22228508041624906
1102	8	0.23534200600029465
1102	9	0.32183122003412024
1102	10-11	0.3233234009964576
1102	12-13	0.33008962840525413
1102	14-15	0.4328240214719514
1102	16-17	0.4874524215792775
1102	18-19	0.42860550011280907
1102	20-21	0.3690439047654266
1102	22-23	0.40445012385793433
1102	24-25	0.3798866098046645
1102	26-27	0.38147819734996347
1102	28-29	0.37640961353346114
1102	30-31	0.4143075637228364
1102	32-33	0.42103455656740607
1102	34-35	0.4314816938540176
1102	36-37	0.4535267751755754
1102	38-39	0.3954769998114003
1102	40-41	0.4544435733442924
1102	42-43	0.44431311364303383
1102	44-45	0.3881614227612502
1102	46-47	0.47385337175974485
1102	48-49	0.531151876363694
1102	50-51	0.5271923940504948
1102	52-53	0.4634348207136867
1102	54-55	0.47940200162263125
1102	56-57	0.4570572328716267
1102	58-59	0.4533047112773403
1102	60-61	0.42917216867846975
1102	62-63	0.4359106154093837
1102	64-65	0.41748222425390935
1102	66-67	0.37369908780714667
1102	68-69	0.35889548879620037
1102	70-71	0.40684945990022214
1102	72-73	0.386805874876643
1102	74-75	0.36658330188521404
1102	76-77	0.3847103047567799
1102	78-79	0.3888106651670853
1102	80-81	0.34498170119497473
1102	82-83	0.32742058017034736
1102	84-85	0.3716984406132582
1102	86-87	0.40689347502983964
1102	88-89	0.4143867350985033
1102	90-91	0.42068853420190067
1102	92-93	0.3655087603009264
1102	94-95	0.4229716681758333
1102	96-97	0.3710950596448228
1102	98-99	0.3280496939726234
1102	100	0.3645107084672716
1103	1	0.19267860729682695
1103	2	0.21554259285633748
1103	3	0.1981600038103437
1103	4	0.15278628387027737
1103	5	0.1678571987260966
1103	6	0.19524447557412827
1103	7	0.18469250267768444
1103	8	0.21862587541554745
1103	9	0.24407928826759218
1103	10-11	0.2527470795077349
1103	12-13	0.2503798612867314
1103	14-15	0.38313417360692625
1103	16-17	0.4425659212183177
1103	18-19	0.3525961688115018
1103	20-21	0.33232563520044067
1103	22-23	0.3624773156442487
1103	24-25	0.36130622425347525
1103	26-27	0.3550129958847634
1103	28-29	0.37095721921245683
1103	30-31	0.4175601505520632
1103	32-33	0.41042588300252447
1103	34-35	0.41350757551493444
1103	36-37	0.42180436133637045
1103	38-39	0.42370151252496413
1103	40-41	0.4576507068434026
1103	42-43	0.4559210920393326
1103	44-45	0.45470442963272006
1103	46-47	0.5278211801071109
1103	48-49	0.6218640230977428
1103	50-51	0.612585164297279
1103	52-53	0.5450810333817913
1103	54-55	0.5304307174323739
1103	56-57	0.5266710132518284
1103	58-59	0.528779297518355
1103	60-61	0.5112675671972298
1103	62-63	0.4700300239813444
1103	64-65	0.4229727191604624
1103	66-67	0.45403584350886916
1103	68-69	0.4313273756441376
1103	70-71	0.47705212827807486
1103	72-73	0.43078798765438364
1103	74-75	0.4444776095240428
1103	76-77	0.3937735650974119
1103	78-79	0.4225143094546695
1103	80-81	0.4595287721289125
1103	82-83	0.44018895534748026
1103	84-85	0.4591934273783238
1103	86-87	0.39007106386436874
1103	88-89	0.4739406240319539
1103	90-91	0.3638671925265413
1103	92-93	0.3769777049815559
1103	94-95	0.4494408802289911
1103	96-97	0.3726404728690724
1103	98-99	0.3617065480674384
1103	100	0.4536313202156208
1104	1	0.15295435432525295
1104	2	0.13984215906032205
1104	3	0.10550947486143514
1104	4	0.11305683892549467
1104	5	0.09911843552067978
1104	6	0.136419951621491
1104	7	0.15224732958196086
1104	8	0.14136185891838693
1104	9	0.1926940631589389
1104	10-11	0.19856243230563564
1104	12-13	0.19810186640015104
1104	14-15	0.29230462941557533
1104	16-17	0.32122398252221274
1104	18-19	0.2818157120643008
1104	20-21	0.29455216631666303
1104	22-23	0.3024475716284982
1104	24-25	0.2751840857462824
1104	26-27	0.234793638344847
1104	28-29	0.24753707675269965
1104	30-31	0.2852108625513168
1104	32-33	0.2963710284742902
1104	34-35	0.2964144348437472
1104	36-37	0.2669817504261687
1104	38-39	0.2587542709604236
1104	40-41	0.24994944632549476
1104	42-43	0.2234339504935008
1104	44-45	0.1858622429866088
1104	46-47	0.19671903069676944
1104	48-49	0.2993437893790656
1104	50-51	0.21077621093917287
1104	52-53	0.20035707661894975
1104	54-55	0.23203605225140933
1104	56-57	0.26612423523730655
1104	58-59	0.2235234397411432
1104	60-61	0.23856912565451438
1104	62-63	0.2760463227834009
1104	64-65	0.2287941525577608
1104	66-67	0.19426423375089286
1104	68-69	0.27211919482502367
1104	70-71	0.19636450467749
1104	72-73	0.17473316507675918
1104	74-75	0.1490259278239563
1104	76-77	0.19917073773518013
1104	78-79	0.2013379481093054
1104	80-81	0.22170756038892847
1104	82-83	0.16883956151035306
1104	84-85	0.170412149899974
1104	86-87	0.21878076200437846
1104	88-89	0.2319839390669145
1104	90-91	0.1320920668708112
1104	92-93	0.24051364508481754
1104	94-95	0.2671786409841985
1104	96-97	0.2181149027338236
1104	98-99	0.24907587508786122
1104	100	0.2600886704854375
1105	1	0.14718515720041836
1105	2	0.1252386124610041
1105	3	0.14139914929825892
1105	4	0.09045717470350212
1105	5	0.12340679085334472
1105	6	0.10874185895907118
1105	7	0.11844439145416885
1105	8	0.14221646847205704
1105	9	0.14489760791936135
1105	10-11	0.16192878559738233
1105	12-13	0.1885239437210373
1105	14-15	0.2697468991339136
1105	16-17	0.33820783493823825
1105	18-19	0.2841662794223794
1105	20-21	0.22778381955532723
1105	22-23	0.2892646709453075
1105	24-25	0.22746971056805165
1105	26-27	0.2882952325050141
1105	28-29	0.25899672713952526
1105	30-31	0.29731465465653173
1105	32-33	0.2945048164841282
1105	34-35	0.33124669397437145
1105	36-37	0.2699555484018248
1105	38-39	0.3114550976608399
1105	40-41	0.3037857617627182
1105	42-43	0.3272729540447443
1105	44-45	0.286568635934735
1105	46-47	0.3031554324090422
1105	48-49	0.3684165394499743
1105	50-51	0.34981169391664224
1105	52-53	0.3193288691721392
1105	54-55	0.346754830190072
1105	56-57	0.3361893527244888
1105	58-59	0.3176162101957374
1105	60-61	0.2766910521217625
1105	62-63	0.3531372189648607
1105	64-65	0.30703685132719016
1105	66-67	0.27411022333377844
1105	68-69	0.2341475409555258
1105	70-71	0.2425440210187375
1105	72-73	0.23273699905525547
1105	74-75	0.23673201709820546
1105	76-77	0.20197581026381073
1105	78-79	0.22883376301586367
1105	80-81	0.23315887826077386
1105	82-83	0.2644008818608867
1105	84-85	0.19390308993408212
1105	86-87	0.22278550917544493
1105	88-89	0.30585431820847475
1105	90-91	0.30128292156209113
1105	92-93	0.35279190600657984
1105	94-95	0.3105761668210576
1105	96-97	0.23454916033994522
1105	98-99	0.24637483129510684
1105	100	0.32149921011443183
1106	1	0.10780063386463468
1106	2	0.10945928526700754
1106	3	0.11996885092039378
1106	4	0.15034759137134301
1106	5	0.10038180263624952
1106	6	0.09090440466778915
1106	7	0.12569828404491545
1106	8	0.11316961815206383
1106	9	0.13810962205695887
1106	10-11	0.1514021393073719
1106	12-13	0.15144584684066587
1106	14-15	0.23018574987536766
1106	16-17	0.34125463210583007
1106	18-19	0.24365067989613465
1106	20-21	0.2265845389472929
1106	22-23	0.18457835717300242
1106	24-25	0.22941841661265983
1106	26-27	0.18319146065960723
1106	28-29	0.19648055798880648
1106	30-31	0.25853779740319993
1106	32-33	0.23702789544791614
1106	34-35	0.2269509092895987
1106	36-37	0.2523990845515769
1106	38-39	0.23754755603967936
1106	40-41	0.20986616207090236
1106	42-43	0.21227636661750182
1106	44-45	0.2037401328852937
1106	46-47	0.2793001730017721
1106	48-49	0.3513743721863989
1106	50-51	0.3095637379324785
1106	52-53	0.29354301316907083
1106	54-55	0.3233875263530379
1106	56-57	0.3412682299574783
1106	58-59	0.32548682359334435
1106	60-61	0.32486959435346563
1106	62-63	0.28898790679464526
1106	64-65	0.3358281702689183
1106	66-67	0.26950136861294993
1106	68-69	0.2869211794307205
1106	70-71	0.29198052317634904
1106	72-73	0.2624753598538021
1106	74-75	0.2976490866413073
1106	76-77	0.24697097010204772
1106	78-79	0.22060631116112717
1106	80-81	0.2094013393207561
1106	82-83	0.2230991676251648
1106	84-85	0.24936068983474513
1106	86-87	0.17883840428833508
1106	88-89	0.2611140429824843
1106	90-91	0.21435209918614362
1106	92-93	0.23448428809054178
1106	94-95	0.3211160424161932
1106	96-97	0.3072538347414664
1106	98-99	0.2851203497944894
1106	100	0.2590559491342219
1107	1	0.10049828941455985
1107	2	0.08802417894483483
1107	3	0.0627344862034036
1107	4	0.04874827724047748
1107	5	0.024394433989037623
1107	6	0.09867567360575435
1107	7	0.08183567627874311
1107	8	0.05557711711841051
1107	9	0.08441030354460821
1107	10-11	0.11978222219082113
1107	12-13	0.112580313821951
1107	14-15	0.18071603738749076
1107	16-17	0.2498958842718224
1107	18-19	0.1279636558960533
1107	20-21	0.13623389396830987
1107	22-23	0.13304254954641692
1107	24-25	0.12171631452317655
1107	26-27	0.13024598823438538
1107	28-29	0.09037014969450041
1107	30-31	0.09771703470462256
1107	32-33	0.029487149310014615
1107	34-35	0.016061788760296736
1107	36-37	-0.009970810935556074
1107	38-39	0.002259293836523568
1107	40-41	0.018501267369984475
1107	42-43	0.11435312077374249
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2216	56-57	-0.4753502179441611
2216	58-59	-0.5778679872357344
2216	60-61	-0.5841785724236743
2216	62-63	-0.5947944719111149
2216	64-65	-0.42504997012436263
2216	66-67	-0.4898520970295621
2216	68-69	-0.4629745198713451
2216	70-71	-0.4090465950051154
2216	72-73	-0.36096438618537263
2216	74-75	-0.34635830213710506
2216	76-77	-0.3096058225052829
2216	78-79	-0.33932181558534324
2216	80-81	-0.3811869361110709
2216	82-83	-0.3629039711435027
2216	84-85	-0.31973043193389117
2216	86-87	-0.31100774405910414
2216	88-89	-0.292444560362938
2216	90-91	-0.37342200694712346
2216	92-93	-0.42445015518417506
2216	94-95	-0.42742618474487415
2216	96-97	-0.35195286259805414
2216	98-99	-0.380445733308143
2216	100	-0.4822298412766095
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	28.0
8	141.0
9	312.0
10	347.0
11	275.0
12	292.0
13	327.0
14	625.0
15	1171.0
16	2183.0
17	3843.0
18	6432.0
19	9901.0
20	14110.0
21	20623.0
22	28957.0
23	32204.0
24	30129.0
25	26306.0
26	24791.0
27	28192.0
28	33867.0
29	41316.0
30	49799.0
31	62734.0
32	79539.0
33	104152.0
34	149146.0
35	231864.0
36	416613.0
37	930134.0
38	1268909.0
39	420036.0
40	1008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44613717646462	19.546494522403645	13.814432835828322	23.192935465303417
2	14.923482300921275	22.152460299673074	38.70099381097518	24.22306358843047
3	19.603979901007413	26.01633559278273	31.203111408822377	23.176573097387486
4	11.916219448007952	17.75532525125331	40.1419237474041	30.186531553334632
5	11.946580939865624	39.15297522792947	35.50913844614701	13.391305386057903
6	28.53623056460588	39.320725730663355	17.575496615270115	14.567547089460648
7	24.857324364202942	34.098059153676786	23.32147188722854	17.723144594891735
8	25.020819383268677	33.21273678628319	20.895621512069273	20.870822318378863
9	25.175092349912582	16.208911409004337	21.97053608095103	36.645460160132046
10-11	24.26846666254592	25.753055333730234	28.85345404721109	21.125023956512756
12-13	26.61480165201273	24.153883234816835	28.156524325996912	21.07479078717352
14-15	22.610912874428855	25.382007565841064	28.21885595041075	23.78822360931934
16-17	20.83017902068993	28.205692649745746	28.548118238748728	22.4160100908156
18-19	20.570817459224795	28.164160202293136	30.22062134845756	21.04440099002451
20-21	22.14767963660396	25.932325397849958	29.7381387494506	22.18185621609548
22-23	21.672108206634917	25.982462040221794	30.100119194847757	22.245310558295532
24-25	22.48932171365975	26.138867967329865	29.954806945139527	21.41700337387086
26-27	21.91596517421907	25.487131238422034	31.210861882733802	21.386041704625093
28-29	20.95436990259699	27.846306264670833	29.9871999824889	21.212123850243277
30-31	21.752708817283896	25.942536086264273	29.815098237767014	22.489656858684818
32-33	21.86058485757886	26.398721984331996	29.8646989835323	21.875994174556844
34-35	22.81370225761703	25.953056748735975	29.805069321923906	21.42817167172309
36-37	22.286111767846556	25.42612111787994	30.84667798338261	21.44108913089089
38-39	21.766523065611203	26.494674564455252	30.355523611965978	21.38327875796757
40-41	21.844238787229354	26.62962448902838	29.830718342643625	21.695418381098637
42-43	21.632770424554536	26.633170891384438	28.745359808253497	22.988698875807525
44-45	22.10799875730265	25.980508356741694	29.069392309554022	22.842100576401634
46-47	23.161148349827922	26.645072964558935	28.683896855663793	21.509881829949347
48-49	22.779505880711273	26.501885615878102	28.560771951477804	22.157836551932817
50-51	21.266293527759565	26.99985921476663	29.454111476036164	22.27973578143764
52-53	22.204039479187536	27.657991806821165	27.6614368272183	22.476531886773
54-55	21.637078667476057	26.618229928620917	28.19014174455584	23.55454965934718
56-57	22.809781803678156	26.19380660878295	27.96459419772373	23.031817389815167
58-59	23.46086868273308	26.099820136503237	27.96410988214476	22.475201298618924
60-61	22.144803613778592	26.78523968296234	29.21467276093615	21.855283942322913
62-63	21.621201792950107	26.883033523729498	28.780490716927577	22.715273966392818
64-65	21.14825959202476	27.896200600304503	28.017634535592308	22.937905272078428
66-67	22.02473600849698	26.548618525826424	27.53257413557979	23.894071330096804
68-69	21.581219701543176	26.56717442583266	28.09889674999807	23.75270912262609
70-71	22.583037533238645	26.37649651631647	27.698704283649693	23.341761666795186
72-73	22.294700939061496	25.670790192687697	29.11264758030976	22.921861287941052
74-75	21.40540978868184	27.87511000712901	27.805001450178775	22.91447875401038
76-77	21.822867992506733	26.285109758533466	27.656404262236446	24.235617986723355
78-79	21.93972345276237	26.5528902592834	27.888900538237205	23.618485749717028
80-81	22.520282523207698	26.253276675468314	27.69294312585605	23.533497675467938
82-83	22.080453718907844	25.726313286378964	28.904581800326916	23.28865119438627
84-85	21.86026768735665	26.587256083956195	28.116015100414472	23.436461128272683
86-87	21.48185182352345	27.007001330122137	27.709870451451785	23.80127639490263
88-89	21.430547112310798	26.68998245905538	26.984213647312593	24.895256781321233
90-91	21.860062161927207	25.625869569894732	27.55149389729365	24.962574370884408
92-93	22.488674045963965	26.319282272735016	27.585779325257075	23.606264356043944
94-95	21.619313069274497	26.011207860302086	27.977850428456456	24.391628641966957
96-97	20.532319344346377	26.032775965237363	28.94970230407307	24.485202386343186
98-99	21.294745372489242	26.7030677282116	27.19793080324274	24.804256096056417
100	21.362966311422074	24.870822034358177	27.312495923905605	26.453715730314144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	370.0
1	239.5
2	381.0
3	643.0
4	422.5
5	306.0
6	703.5
7	1832.5
8	3430.0
9	4057.5
10	2992.0
11	1992.5
12	2088.0
13	2398.5
14	2719.5
15	3168.0
16	3680.5
17	4214.0
18	4974.5
19	5783.0
20	6562.5
21	7592.5
22	8948.5
23	10842.5
24	13084.0
25	15948.0
26	20271.5
27	25875.5
28	31771.0
29	39285.0
30	48349.5
31	56487.5
32	65170.5
33	72546.0
34	79021.5
35	86212.0
36	93750.5
37	103638.5
38	109296.0
39	110688.0
40	113581.5
41	115060.5
42	117324.5
43	121807.5
44	126763.5
45	137639.0
46	151581.0
47	192185.0
48	259777.5
49	245087.0
50	177482.0
51	150315.5
52	137303.0
53	129032.0
54	122512.5
55	111537.5
56	100812.5
57	88682.0
58	76425.0
59	68059.0
60	56203.5
61	43583.5
62	34535.0
63	26585.0
64	18451.0
65	12486.0
66	9058.0
67	6463.0
68	4541.0
69	3271.5
70	2421.5
71	1885.0
72	1559.0
73	1305.0
74	995.5
75	736.5
76	578.5
77	383.0
78	232.0
79	162.5
80	124.5
81	72.5
82	41.5
83	34.0
84	30.0
85	17.5
86	11.0
87	9.0
88	4.0
89	3.5
90	1.5
91	0.5
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002835603640318105
2	0.0031589619501789417
3	1.741160130019889E-4
4	0.04857836762755491
5	0.0023630030335984207
6	0.004825500931769407
7	0.0
8	3.9797945829026036E-4
9	2.4873716143141273E-5
10-11	6.218429035785317E-5
12-13	0.0
14-15	3.7310574214711905E-5
16-17	4.601637486481135E-4
18-19	8.705800650099445E-5
20-21	2.7361087757455396E-4
22-23	0.0
24-25	0.0
26-27	2.6117401950298335E-4
28-29	2.4873716143141273E-5
30-31	2.7361087757455396E-4
32-33	3.233583098608365E-4
34-35	1.741160130019889E-4
36-37	3.7310574214711905E-5
38-39	6.591534777932438E-4
40-41	6.46716619721673E-4
42-43	0.0
44-45	5.223480390059667E-4
46-47	0.0
48-49	8.705800650099445E-5
50-51	0.0
52-53	3.109214517892659E-4
54-55	3.109214517892659E-4
56-57	1.6167915493041826E-4
58-59	9.20327497296227E-4
60-61	7.089009100795262E-4
62-63	1.6167915493041826E-4
64-65	3.731057421471191E-4
66-67	0.0013804912459443406
68-69	0.0023630030335984207
70-71	0.003432572827753495
72-73	0.003084340801749518
74-75	0.002835603640318105
76-77	0.00410416316361831
78-79	0.0013556175298011994
80-81	9.078906392246563E-4
82-83	5.347848970775373E-4
84-85	5.223480390059667E-4
86-87	1.2436858071570636E-5
88-89	0.0
90-91	3.7310574214711905E-5
92-93	1.6167915493041826E-4
94-95	1.1193172264413572E-4
96-97	3.606688840755484E-4
98-99	9.949486457256509E-5
100	0.07417342153884728
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4020308.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.62360525824074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.29501850868319	34.57340058087822
2	16.005435625630312	16.205057101961845
3	6.468958798265375	9.824460499056292
4	3.3318392855933507	6.746788671111063
5	1.8516209998041293	4.686786529097664
6	1.1730130724548848	3.5629290445627335
7	0.7430122481873364	2.6329771127991597
8	0.499061682912146	2.0211441308206224
9	0.34710787350885425	1.5814666773485635
>10	1.2467214586964093	9.690537185930246
>50	0.023991281394124816	0.8052009553296609
>100	0.01165915423736626	1.1078355790135146
>500	0.0010860651168599484	0.38317503456286717
>1k	7.757607977526954E-4	0.673040252363491
>5k	2.3272823932580865E-4	0.9292888021751248
>10k+	4.654564786516173E-4	4.5759118429887735
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	49563	1.232815993202511	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14983	0.37268288897268564	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	14060	0.34972444897256627	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	13570	0.33753632806242706	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13101	0.32587055519129376	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	11242	0.27963031688119416	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	8322	0.20699906574322166	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	7758	0.19297028983849	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7583	0.18861738951344026	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.4873716143141273E-5	0.0
9	0.0	0.0	2.4873716143141273E-5	2.4873716143141273E-5	0.0
10-11	1.2436858071570636E-5	0.0	2.4873716143141273E-5	2.4873716143141273E-5	0.0
12-13	2.4873716143141273E-5	0.0	2.4873716143141273E-5	2.4873716143141273E-5	0.0
14-15	2.4873716143141273E-5	0.0	2.4873716143141273E-5	4.9747432286282545E-5	1.2436858071570636E-5
16-17	2.4873716143141273E-5	0.0	2.4873716143141273E-5	7.462114842942381E-5	2.4873716143141273E-5
18-19	2.4873716143141273E-5	0.0	2.4873716143141273E-5	1.741160130019889E-4	2.4873716143141273E-5
20-21	2.4873716143141273E-5	0.0	2.4873716143141273E-5	3.7310574214711903E-4	2.4873716143141273E-5
22-23	2.4873716143141273E-5	0.0	2.4873716143141273E-5	6.964640520079556E-4	2.4873716143141273E-5
24-25	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.0011566278006560691	2.4873716143141273E-5
26-27	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.0025495559046719803	2.4873716143141273E-5
28-29	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.008357568624095467	2.4873716143141273E-5
30-31	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.02288381885168997	2.4873716143141273E-5
32-33	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.0565006462191454	2.4873716143141273E-5
34-35	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.1067331159702192	2.4873716143141273E-5
36-37	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.17676506377123347	2.4873716143141273E-5
38-39	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.28455531267753614	2.4873716143141273E-5
40-41	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.40835179792195025	2.4873716143141273E-5
42-43	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.5348719550840384	2.4873716143141273E-5
44-45	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.6950462501877965	2.4873716143141273E-5
46-47	2.4873716143141273E-5	0.0	2.4873716143141273E-5	0.8949065593979366	2.4873716143141273E-5
48-49	2.4873716143141273E-5	0.0	2.4873716143141273E-5	1.0974407930934644	2.4873716143141273E-5
50-51	2.4873716143141273E-5	0.0	2.4873716143141273E-5	1.314551024448873	2.4873716143141273E-5
52-53	2.4873716143141273E-5	0.0	2.4873716143141273E-5	1.53275569931458	2.4873716143141273E-5
54-55	2.4873716143141273E-5	0.0	2.4873716143141273E-5	1.7778861719052372	2.4873716143141273E-5
56-57	2.4873716143141273E-5	0.0	2.4873716143141273E-5	2.0829996109750795	3.7310574214711905E-5
58-59	2.4873716143141273E-5	0.0	2.4873716143141273E-5	2.4000773075097728	4.9747432286282545E-5
60-61	2.4873716143141273E-5	0.0	2.4873716143141273E-5	2.7432724059947646	4.9747432286282545E-5
62-63	2.4873716143141273E-5	0.0	2.4873716143141273E-5	3.0851616343822412	4.9747432286282545E-5
64-65	2.4873716143141273E-5	0.0	2.4873716143141273E-5	3.435545236832601	4.9747432286282545E-5
66-67	2.4873716143141273E-5	0.0	2.4873716143141273E-5	3.84903345713811	4.9747432286282545E-5
68-69	2.4873716143141273E-5	0.0	2.4873716143141273E-5	4.318748712785189	4.9747432286282545E-5
70-71	2.4873716143141273E-5	0.0	2.4873716143141273E-5	4.814655991530998	4.9747432286282545E-5
72-73	2.4873716143141273E-5	0.0	2.4873716143141273E-5	5.309618069063365	4.9747432286282545E-5
74-75	2.4873716143141273E-5	0.0	2.4873716143141273E-5	5.81587281372472	4.9747432286282545E-5
76-77	2.4873716143141273E-5	0.0	2.4873716143141273E-5	6.345471540986412	4.9747432286282545E-5
78-79	2.4873716143141273E-5	0.0	2.4873716143141273E-5	6.987014427750312	4.9747432286282545E-5
80-81	2.4873716143141273E-5	0.0	2.4873716143141273E-5	7.664947561231627	4.9747432286282545E-5
82-83	2.4873716143141273E-5	0.0	2.4873716143141273E-5	8.328727550227494	4.9747432286282545E-5
84-85	4.9747432286282545E-5	0.0	2.4873716143141273E-5	8.996076917489905	4.9747432286282545E-5
86-87	4.9747432286282545E-5	0.0	2.4873716143141273E-5	9.675402979075233	4.9747432286282545E-5
88	4.9747432286282545E-5	0.0	2.4873716143141273E-5	10.216779410930705	4.9747432286282545E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	11400	0.0	23.185572	1
GTACTAG	1335	0.0	22.899103	1
GAGTACT	17960	0.0	22.517502	12-13
TACATGG	11440	0.0	22.200056	2
AGAGTAC	23615	0.0	21.682646	10-11
AGTACTT	18630	0.0	21.10225	12-13
ACATGGG	11490	0.0	20.710722	3
GTACTTT	19755	0.0	20.186388	14-15
CTAACGC	525	0.0	19.707346	3
CATGGGG	8180	0.0	19.662445	4
TATTCGC	200	1.4095704E-5	18.798923	9
TAGTACT	1810	0.0	18.447798	4
TAACGCC	600	0.0	18.027742	4
ACTTTTT	22275	0.0	17.945095	16-17
TACTTTT	21945	0.0	17.818516	14-15
GTACCGT	335	1.6636477E-8	16.836113	6
ATGGGGG	5545	0.0	16.698423	5
TGATCGC	790	0.0	16.657274	9
CTGTGCG	1705	0.0	16.538641	9
TCTAACG	600	0.0	16.460932	2
>>END_MODULE