FastQCFastQC Report
Mon 16 Jan 2017
ERR1146521_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146521_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492505
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT675291.933540538954132No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT188250.5390113972635686No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC178970.5124402112523818No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC142950.40930506899775376No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA136310.39029292728285286No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG110120.3153037719344711No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA104260.2985249842162001No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC99090.28372185580264025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11550.024.4296421
GTACATG94300.024.336381
TACATGG93900.023.3819542
ACATGGG92350.022.4504453
TCTAACG6250.020.3101562
GTGATCG8200.020.0581448
CATGGGG67600.019.9599064
TAGGCAT17950.019.6429925
CTAGTAC17400.019.4538963
CTGTGCG15300.019.3493779
ACGCCTA6650.019.0805596
TGATCGC8750.018.7965379
TAACGCC6800.018.6671564
TAGTACT19050.018.0157014
CTAACGC7350.017.9099333
TCGCAAT1851.6470709E-417.7891772
GCTATTG17850.017.6389668
GGCTATT19750.017.36977
GTATAGA8150.017.3105741
GGGCTAT19900.017.003116
Produced by FastQC (version 0.11.5)