FastQCFastQC Report
Mon 16 Jan 2017
ERR1146521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492505
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT685211.961944220552297No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT188960.5410443220553729No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC164630.4713808570066471No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA146830.4204145734938103No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG120030.3436788207890898No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114210.32701456404500495No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC108450.3105221037621993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98730.2826910770349649No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT58160.1665280364666622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57040.16332116918945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12700.025.554261
GAGTACT139050.022.81437712-13
GTACATG94800.022.574651
TGATCGC7850.022.1514249
TACATGG95400.021.7421232
ACATGGG92650.021.5232223
AGAGTAC188400.021.19133610-11
GTGATCG8400.020.7010358
CTGTGCG13750.020.5077579
GTACTTT154800.020.35681714-15
TATATCG1405.212213E-420.1435645
GGGCTAT19700.020.040746
AGTACTT148750.020.03125412-13
TAGTACT15200.019.8026454
CATGGGG67850.019.5472684
CTATTGA18850.018.948379
GGCGTCG11050.018.7137158
GCTATTG18650.018.6475818
TACGGGC11800.018.3342294
TATTCTC15200.018.2440625
Produced by FastQC (version 0.11.5)