##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146521_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3492505
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26205832203533	33.0	31.0	34.0	30.0	34.0
2	32.33726021866826	34.0	31.0	34.0	30.0	34.0
3	32.49294560780872	34.0	31.0	34.0	30.0	34.0
4	35.988917696610315	37.0	35.0	37.0	35.0	37.0
5	36.032310333127654	37.0	35.0	37.0	35.0	37.0
6	36.12911162618235	37.0	36.0	37.0	35.0	37.0
7	36.118204841510604	37.0	36.0	37.0	35.0	37.0
8	36.124745419118945	37.0	36.0	37.0	35.0	37.0
9	37.90788331011695	39.0	38.0	39.0	35.0	39.0
10-11	37.86185646119333	39.0	38.0	39.0	35.0	39.0
12-13	37.841562861041	39.0	38.0	39.0	35.0	39.0
14-15	39.13745205805002	40.0	39.0	41.0	36.0	41.0
16-17	39.1246675380565	40.0	39.0	41.0	36.0	41.0
18-19	39.25562941212682	40.5	39.0	41.0	36.0	41.0
20-21	39.304883600739295	41.0	39.0	41.0	36.0	41.0
22-23	39.25979003609157	40.5	39.0	41.0	36.0	41.0
24-25	39.227902608586106	41.0	39.0	41.0	36.0	41.0
26-27	39.132705894479756	40.0	39.0	41.0	35.5	41.0
28-29	39.09415462540497	40.0	39.0	41.0	35.0	41.0
30-31	38.98454490401589	40.0	38.5	41.0	35.0	41.0
32-33	38.861035417272134	40.0	38.0	41.0	35.0	41.0
34-35	38.75380550636291	40.0	38.0	41.0	35.0	41.0
36-37	38.654851460484664	40.0	38.0	41.0	35.0	41.0
38-39	38.56600606155181	40.0	38.0	41.0	35.0	41.0
40-41	38.52700425625733	40.0	38.0	41.0	35.0	41.0
42-43	38.41689074174553	40.0	38.0	41.0	34.0	41.0
44-45	38.26656425688725	40.0	38.0	41.0	34.0	41.0
46-47	38.00831623719938	40.0	38.0	41.0	33.0	41.0
48-49	37.700484179693376	40.0	37.0	41.0	33.0	41.0
50-51	37.57237956710155	40.0	37.0	41.0	33.0	41.0
52-53	37.54006508222608	40.0	37.0	41.0	33.0	41.0
54-55	37.393461856174866	40.0	37.0	41.0	32.0	41.0
56-57	37.21550133786494	40.0	36.0	41.0	32.0	41.0
58-59	37.032554140938956	39.0	36.0	41.0	32.0	41.0
60-61	36.75327393947897	39.0	35.0	41.0	31.0	41.0
62-63	36.572453869071055	39.0	35.0	41.0	31.0	41.0
64-65	36.300585682769245	38.0	35.0	40.5	31.0	41.0
66-67	35.98724411275002	37.0	35.0	40.0	31.0	41.0
68-69	35.62581671321874	37.0	35.0	39.5	30.5	41.0
70-71	35.22714942426711	36.0	35.0	39.0	30.0	41.0
72-73	34.877940189062	36.0	34.5	39.0	29.5	41.0
74-75	34.586098373517004	35.0	34.0	37.5	30.0	40.0
76-77	34.304114382083924	35.0	34.0	37.0	29.5	39.0
78-79	34.008894332291575	35.0	34.0	37.0	29.0	39.0
80-81	33.74343758992471	35.0	34.0	36.0	29.0	39.0
82-83	33.46884328010984	35.0	34.0	36.0	29.0	37.0
84-85	33.27673446423126	35.0	34.0	35.5	29.0	37.0
86-87	33.17130197379818	35.0	34.0	35.0	29.0	36.5
88-89	32.977124155870925	35.0	34.0	35.0	29.0	36.0
90-91	32.8764885089642	35.0	34.0	35.0	29.0	36.0
92-93	32.77954333637318	35.0	34.0	35.0	29.0	36.0
94-95	32.69346643741383	35.0	34.0	35.0	28.5	35.5
96-97	32.637695006879014	35.0	34.0	35.0	28.0	35.0
98-99	32.613287454133925	35.0	34.0	35.0	27.0	35.0
100	31.829833887138314	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34319460015532854
1101	2	0.3263485083550677
1101	3	0.3229440080236259
1101	4	0.2646623735193785
1101	5	0.28358283801583184
1101	6	0.22031909343338185
1101	7	0.22702494333227463
1101	8	0.24647388430761907
1101	9	0.29064921590268966
1101	10-11	0.3207166482975481
1101	12-13	0.3365900603580343
1101	14-15	0.47444177285233735
1101	16-17	0.5163328080601772
1101	18-19	0.49967169691862523
1101	20-21	0.4486292415337303
1101	22-23	0.42095548300547847
1101	24-25	0.4281122506704449
1101	26-27	0.43345283329289686
1101	28-29	0.4663879092656842
1101	30-31	0.5254665324538976
1101	32-33	0.5168604507854155
1101	34-35	0.5409633237932425
1101	36-37	0.5228838956746813
1101	38-39	0.614619082324694
1101	40-41	0.5916295313168263
1101	42-43	0.5535007718870091
1101	44-45	0.5403982368558786
1101	46-47	0.5864297883776999
1101	48-49	0.6942242887991625
1101	50-51	0.6409839708198461
1101	52-53	0.6630799423536757
1101	54-55	0.6418946004965846
1101	56-57	0.6308355938787358
1101	58-59	0.5640003398403834
1101	60-61	0.5784407545371835
1101	62-63	0.5988987149275999
1101	64-65	0.4855170509176858
1101	66-67	0.531297374608279
1101	68-69	0.5218122742186608
1101	70-71	0.519383004345535
1101	72-73	0.5542009318446048
1101	74-75	0.5680494473459134
1101	76-77	0.49338338354015576
1101	78-79	0.48567147704756763
1101	80-81	0.5018599565842905
1101	82-83	0.4887866986261713
1101	84-85	0.5000618376342558
1101	86-87	0.470282038121276
1101	88-89	0.5363337574008114
1101	90-91	0.4422958787947948
1101	92-93	0.500897037535097
1101	94-95	0.48430948256741146
1101	96-97	0.4634117969173488
1101	98-99	0.46934285203325743
1101	100	0.619251621430287
1102	1	0.15809105074930585
1102	2	0.19993718400102978
1102	3	0.24764433777639283
1102	4	0.118796797048077
1102	5	0.15188032906507942
1102	6	0.16368690743621528
1102	7	0.16724663275866192
1102	8	0.17911724542858565
1102	9	0.2284162613478955
1102	10-11	0.2219274445248729
1102	12-13	0.2387490695976595
1102	14-15	0.3382840855740952
1102	16-17	0.4007697303032245
1102	18-19	0.3407155081822566
1102	20-21	0.32732894304835014
1102	22-23	0.35923464770090874
1102	24-25	0.30504624714073003
1102	26-27	0.32256635716323245
1102	28-29	0.3267763573983373
1102	30-31	0.3753015165279123
1102	32-33	0.3743113896765209
1102	34-35	0.36615710406606894
1102	36-37	0.38586147141996463
1102	38-39	0.40578825465954793
1102	40-41	0.3591760071604284
1102	42-43	0.3502347807067636
1102	44-45	0.3206266412441039
1102	46-47	0.3829258531180244
1102	48-49	0.5122846555784122
1102	50-51	0.4424733975956343
1102	52-53	0.36200941576840506
1102	54-55	0.325314699901341
1102	56-57	0.3470097461197881
1102	58-59	0.3601390274744958
1102	60-61	0.3433179906919861
1102	62-63	0.35543482623708655
1102	64-65	0.36341184410119354
1102	66-67	0.329468466991635
1102	68-69	0.3108046656396297
1102	70-71	0.25300631432335763
1102	72-73	0.3385156252366315
1102	74-75	0.2684988324863582
1102	76-77	0.2654680652191175
1102	78-79	0.21572091629044365
1102	80-81	0.3240158321086142
1102	82-83	0.3445414792239916
1102	84-85	0.23663833041377558
1102	86-87	0.2446322594125121
1102	88-89	0.31996940438917676
1102	90-91	0.3008068138315565
1102	92-93	0.3114660904319493
1102	94-95	0.2860890072563578
1102	96-97	0.3509613287314082
1102	98-99	0.330039413951873
1102	100	0.4173839410638607
1103	1	0.23399945602118777
1103	2	0.24259049957508694
1103	3	0.2345772830301911
1103	4	0.1601953198596462
1103	5	0.14545534898125112
1103	6	0.21796921731882435
1103	7	0.1588362914757937
1103	8	0.18844638101311517
1103	9	0.3007588012831022
1103	10-11	0.28462519289497124
1103	12-13	0.2722445620005942
1103	14-15	0.3970244106163463
1103	16-17	0.49654898538477
1103	18-19	0.44053668434276005
1103	20-21	0.38928539568308196
1103	22-23	0.40107106672746085
1103	24-25	0.40371610379251166
1103	26-27	0.4017702210667622
1103	28-29	0.369825123374369
1103	30-31	0.396625648881745
1103	32-33	0.3747560976104083
1103	34-35	0.37334666484272816
1103	36-37	0.3028280545171924
1103	38-39	0.28265821051219575
1103	40-41	0.29084524528960287
1103	42-43	0.3580780579757956
1103	44-45	0.43342214936497214
1103	46-47	0.41494739612898
1103	48-49	0.44040978416698806
1103	50-51	0.38798599473445705
1103	52-53	0.4066060869416148
1103	54-55	0.38697516713151003
1103	56-57	0.25307357072109227
1103	58-59	0.35394082665798265
1103	60-61	0.36833483927016175
1103	62-63	0.41603197024554106
1103	64-65	0.3623560740099592
1103	66-67	0.379314610187123
1103	68-69	0.37306845654377696
1103	70-71	0.3516540676423716
1103	72-73	0.3650309824654059
1103	74-75	0.38423241568774813
1103	76-77	0.30247022587781913
1103	78-79	0.28744824679727543
1103	80-81	0.3635505388821798
1103	82-83	0.37660134394603517
1103	84-85	0.3876873860963812
1103	86-87	0.4028129530920097
1103	88-89	0.39772480492381135
1103	90-91	0.35132583437496123
1103	92-93	0.3362676979605368
1103	94-95	0.30577390177388253
1103	96-97	0.2990067500144491
1103	98-99	0.35499136584760294
1103	100	0.3827842087875837
1104	1	0.18623681555911276
1104	2	0.19178075159894092
1104	3	0.17378641556466334
1104	4	0.16147036845351437
1104	5	0.17695210258522565
1104	6	0.15859706954915964
1104	7	0.1834213492659984
1104	8	0.16019282305579452
1104	9	0.1988693489736022
1104	10-11	0.19721745897835063
1104	12-13	0.1944673762450151
1104	14-15	0.3554377246113134
1104	16-17	0.375115505883457
1104	18-19	0.26663250302065933
1104	20-21	0.2865415717240367
1104	22-23	0.27777245780279003
1104	24-25	0.25433150222075795
1104	26-27	0.2613377736446765
1104	28-29	0.26605393426548574
1104	30-31	0.28060232486006953
1104	32-33	0.30517231651222687
1104	34-35	0.3156535054901113
1104	36-37	0.34140430763021357
1104	38-39	0.32121400714380854
1104	40-41	0.3593602725430003
1104	42-43	0.3643944399066896
1104	44-45	0.34815430179853024
1104	46-47	0.4921141310704087
1104	48-49	0.5774007853910845
1104	50-51	0.5028658749675969
1104	52-53	0.41886436312366726
1104	54-55	0.42065639616036776
1104	56-57	0.44165028226688463
1104	58-59	0.42124412093860997
1104	60-61	0.38345611463360996
1104	62-63	0.3586740722964805
1104	64-65	0.3288377183616191
1104	66-67	0.33390360011959075
1104	68-69	0.34023956649082976
1104	70-71	0.3583903047984762
1104	72-73	0.3287662252548671
1104	74-75	0.2709403995453812
1104	76-77	0.32867359461484824
1104	78-79	0.35321047172895703
1104	80-81	0.33620727272338513
1104	82-83	0.3289799959253159
1104	84-85	0.27930579045969495
1104	86-87	0.30283538169932456
1104	88-89	0.42473332865955626
1104	90-91	0.3905217069576352
1104	92-93	0.31887233653905867
1104	94-95	0.3651086829474792
1104	96-97	0.3352068076558865
1104	98-99	0.3082476138538581
1104	100	0.29484901768858407
1105	1	0.2091153854569754
1105	2	0.16806484122970744
1105	3	0.16146614360401657
1105	4	0.12773927035031107
1105	5	0.14010924805184288
1105	6	0.11473500994906516
1105	7	0.1438571382006728
1105	8	0.1323224351489074
1105	9	0.21662602365496753
1105	10-11	0.20280335372905967
1105	12-13	0.19278928771696258
1105	14-15	0.31308293254932806
1105	16-17	0.4140640377989442
1105	18-19	0.3216537104013355
1105	20-21	0.27764699576689367
1105	22-23	0.2773177555075321
1105	24-25	0.2949175471576311
1105	26-27	0.28239637746526114
1105	28-29	0.2878534775480901
1105	30-31	0.34439194340319546
1105	32-33	0.3622537247351403
1105	34-35	0.3189671929021287
1105	36-37	0.36896147113599653
1105	38-39	0.29265724320895004
1105	40-41	0.369042463467224
1105	42-43	0.3580489481126179
1105	44-45	0.3946069886543171
1105	46-47	0.44000964844661894
1105	48-49	0.4997090390927923
1105	50-51	0.4112403019568802
1105	52-53	0.34309893321975693
1105	54-55	0.43950444469515304
1105	56-57	0.40379728903132417
1105	58-59	0.39828290657953147
1105	60-61	0.35843554081137796
1105	62-63	0.45157746876718363
1105	64-65	0.4522749934241759
1105	66-67	0.35315599441103274
1105	68-69	0.343719447307798
1105	70-71	0.36579850045343676
1105	72-73	0.3766950906913422
1105	74-75	0.3527121823662469
1105	76-77	0.29176317734046364
1105	78-79	0.24777668990410717
1105	80-81	0.3136829444573124
1105	82-83	0.25598154633456716
1105	84-85	0.2858143835905551
1105	86-87	0.3247689777056024
1105	88-89	0.3696172978553207
1105	90-91	0.3912572405589927
1105	92-93	0.40747098516644087
1105	94-95	0.3390112280266777
1105	96-97	0.34934885465884946
1105	98-99	0.3813682947920469
1105	100	0.36876505428151063
1106	1	0.07182064674916688
1106	2	0.05197272389705887
1106	3	0.05593189541563248
1106	4	0.10288441110193958
1106	5	0.07703539804148818
1106	6	0.10335929497792051
1106	7	0.13878148960820624
1106	8	0.11364796849350967
1106	9	0.1506474574057961
1106	10-11	0.1552220868673544
1106	12-13	0.15555416102813524
1106	14-15	0.21345904432063634
1106	16-17	0.2579158123910972
1106	18-19	0.1791760991506095
1106	20-21	0.19156533915788287
1106	22-23	0.20374924635384417
1106	24-25	0.17578566660491646
1106	26-27	0.19364726699473778
1106	28-29	0.19195014796333254
1106	30-31	0.2097733264224786
1106	32-33	0.17389481131751694
1106	34-35	0.1461739670844011
1106	36-37	0.12916611453611893
1106	38-39	0.1938455380741999
1106	40-41	0.17538063574555451
1106	42-43	0.14225938835227936
1106	44-45	0.07454282191729078
1106	46-47	0.15974376166978743
1106	48-49	0.1998485631084037
1106	50-51	0.19821538245662396
1106	52-53	0.14850024006403828
1106	54-55	0.24764160794397583
1106	56-57	0.14700915435803807
1106	58-59	0.18372735240956928
1106	60-61	0.23785473657655842
1106	62-63	0.1753687154904071
1106	64-65	0.16925023133561723
1106	66-67	0.18046682386648882
1106	68-69	0.19032028453366934
1106	70-71	0.17097239848458656
1106	72-73	0.21345348477299098
1106	74-75	0.20257475376625678
1106	76-77	0.14321617941686782
1106	78-79	0.16369381447871234
1106	80-81	0.15903798043611062
1106	82-83	0.1526825648701049
1106	84-85	0.10381091685318466
1106	86-87	0.15400127261909802
1106	88-89	0.15461538949387688
1106	90-91	0.0989161788558448
1106	92-93	0.16862181604473392
1106	94-95	0.16859172990836413
1106	96-97	0.1079138608909247
1106	98-99	0.17776892272166833
1106	100	0.2459876002993937
1107	1	0.0839271842794389
1107	2	0.14166984873875066
1107	3	0.12556854531150208
1107	4	0.11211328817727662
1107	5	0.10192790199680246
1107	6	0.08690652972156698
1107	7	0.07234508453770161
1107	8	0.10840982192934234
1107	9	0.14078611479271075
1107	10-11	0.10006025575836475
1107	12-13	0.11381643982879552
1107	14-15	0.2245379697066383
1107	16-17	0.278127667174239
1107	18-19	0.17561313938126943
1107	20-21	0.18052952767342134
1107	22-23	0.178893408462109
1107	24-25	0.14867327790229723
1107	26-27	0.1592502649506784
1107	28-29	0.1669747844784979
1107	30-31	0.13454072307422393
1107	32-33	0.12773502811884896
1107	34-35	0.08860853596463159
1107	36-37	0.10364778535378605
1107	38-39	0.11239616033867605
1107	40-41	0.1317434007324465
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2216	52-53	-0.6894655195570536
2216	54-55	-0.6486583792963714
2216	56-57	-0.7023100052119844
2216	58-59	-0.8011836955305682
2216	60-61	-0.7161291747530711
2216	62-63	-0.7869441636950185
2216	64-65	-0.7162434773061719
2216	66-67	-0.7391292989537561
2216	68-69	-0.7259275409893107
2216	70-71	-0.7643364616361339
2216	72-73	-0.702397762154213
2216	74-75	-0.6965163591103831
2216	76-77	-0.695527404617728
2216	78-79	-0.6197690805768232
2216	80-81	-0.5986653631538914
2216	82-83	-0.656320364099173
2216	84-85	-0.6139057827519636
2216	86-87	-0.584980870067767
2216	88-89	-0.5340952358791284
2216	90-91	-0.634255930745212
2216	92-93	-0.6533315586876114
2216	94-95	-0.5804403146895822
2216	96-97	-0.5681170413923553
2216	98-99	-0.6143181529270478
2216	100	-0.9006968340247816
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	21.0
8	130.0
9	254.0
10	330.0
11	225.0
12	185.0
13	271.0
14	456.0
15	904.0
16	1758.0
17	3110.0
18	5123.0
19	7960.0
20	11323.0
21	16668.0
22	23561.0
23	25845.0
24	24189.0
25	21274.0
26	20218.0
27	23208.0
28	28853.0
29	35854.0
30	43353.0
31	54454.0
32	69496.0
33	91050.0
34	130346.0
35	203331.0
36	374511.0
37	851212.0
38	1087145.0
39	335167.0
40	719.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9880781773321	19.53605795868136	13.711499107065904	22.76436475692063
2	14.88759050099288	22.673632337971547	37.497168823991764	24.941608337043807
3	20.88861375932857	25.332999665568696	31.0511164508134	22.727270124289333
4	11.915908934002562	17.420369675949864	40.36877643923849	30.29494495080909
5	11.937855268598236	39.430791415027024	35.24771305235212	13.383640264022622
6	28.722162329323137	38.998953425277286	17.208523212912812	15.070361032486767
7	24.907938571312	33.940967872630104	23.080138754275225	18.070954801782673
8	24.919348488687888	31.93754035438848	20.803322553074523	22.33978860384911
9	24.493307812014585	17.003297060419385	22.588972671477922	35.914422456088104
10-11	24.718715063523987	25.244742339977428	28.434334779432323	21.602207817066258
12-13	27.461492539022853	23.848169150795776	27.442895572089377	21.247442738092
14-15	22.892128398421306	25.287029019866548	28.545536383064984	23.27530619864716
16-17	20.901970322965063	28.06538070095091	28.445796039711972	22.586852936372058
18-19	20.45613164550728	28.92053988252829	29.430446752978273	21.192881718986154
20-21	22.564344768474225	25.84655466171815	28.69158419576609	22.897516374041533
22-23	21.721658236709754	25.979003609157324	29.371697391986558	22.927640762146368
24-25	23.28788362507713	26.054508153889543	28.971168258885815	21.686439962147514
26-27	22.44686105491919	24.922526732321735	31.20315132877785	21.427460883981222
28-29	20.90038525356442	28.60748946672947	29.17012287741893	21.322002402287183
30-31	22.043580560902335	25.81132842614776	28.94315600798569	23.201935004964213
32-33	22.371555026166803	26.294693124332944	29.258317292997347	22.075434556502906
34-35	23.645793202656037	25.757534760593177	29.13131051097689	21.4653615257739
36-37	22.640414636545206	24.944291410361817	30.792344004138005	21.622949948954968
38-39	22.037969040311502	26.57474909641495	29.999281312375143	21.388000550898404
40-41	22.06908399815318	26.81084756924685	29.192659959412882	21.92740847318709
42-43	21.61568845284402	26.843984475326447	27.707132845908593	23.833194225920938
44-45	22.485299522102935	25.44573876191718	28.43143220085048	23.637529515129398
46-47	23.937302881456148	26.64497259130624	28.13781225796384	21.279912269273773
48-49	23.30089526101782	26.259262345236266	28.201981903512696	22.237860490233217
50-51	20.877736753419107	26.94328282994584	29.758482808184954	22.420497608450095
52-53	22.365605806619993	28.019856283320543	26.832653948257356	22.78188396180211
54-55	21.60523396747425	26.224740829905507	27.871987754309668	24.298037448310577
56-57	23.31010306804713	25.571292299767627	27.488644590684864	23.629960041500375
58-59	24.564453171137107	25.708311649824516	27.296911719545797	22.43032345949258
60-61	22.559225422031464	26.58928140656396	29.265558238301836	21.58593493310274
62-63	21.673563829223717	26.644059167948225	28.61323653922306	23.069140463605002
64-65	21.056588182205505	28.483769201167302	27.404269038532902	23.05537357809429
66-67	22.41689534952973	26.17306864150926	26.82193717673253	24.58809883222848
68-69	21.64999772364344	26.261681359904	27.730274940922108	24.358045975530455
70-71	23.27437225268609	26.192833357457225	26.952213363433074	23.580581026423605
72-73	22.57763725723615	24.918369441859873	29.43052386581593	23.073469435088047
74-75	21.345632551897438	28.35393081639449	27.341465810067927	22.958970821640143
76-77	22.032032979328218	25.940124605944476	27.163577622585468	24.864264792141842
78-79	22.214119049071158	26.342041524324266	27.62858490249993	23.81525452410465
80-81	23.169644525272297	25.827791530379425	27.44043220980678	23.562131734541495
82-83	22.471774347104162	25.103300549552337	29.217197870051614	23.207727233291884
84-85	21.959408868236302	26.506483775029082	28.064857506985707	23.469249849748905
86-87	21.605323399680174	27.101464421668688	27.459373716000407	23.833838462650732
88-89	21.461501128845914	26.769396178387716	26.327521363605776	25.44158132916059
90-91	22.146234069629422	25.201477965591156	27.192084417381402	25.46020354739802
92-93	23.18664488286914	26.2869133435495	27.284764741858563	23.241677031722798
94-95	21.97762206326266	25.9242334114292	27.767582127715013	24.33056239759313
96-97	20.273438070420223	26.132694400644123	29.273203567310553	24.320663961625097
98-99	21.567598916592853	27.0323225436904	26.66247291875835	24.737605620958394
100	21.663468324665764	24.32334077113807	26.949601255534294	27.063589648661868
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	133.0
1	97.5
2	251.5
3	429.0
4	278.5
5	235.5
6	507.0
7	1240.0
8	2342.5
9	2816.0
10	2113.5
11	1438.5
12	1503.5
13	1778.5
14	2060.0
15	2314.5
16	2693.5
17	3104.5
18	3542.5
19	4149.0
20	4835.5
21	5559.0
22	6606.5
23	8013.5
24	9761.5
25	12513.0
26	16430.5
27	20713.5
28	24881.0
29	30369.0
30	36756.0
31	42591.0
32	50208.0
33	56329.5
34	61090.0
35	67561.5
36	74662.5
37	82701.0
38	87434.0
39	89593.5
40	90508.5
41	90726.0
42	92848.0
43	96870.0
44	102934.5
45	112572.0
46	126440.5
47	183247.5
48	280491.0
49	267336.0
50	174018.0
51	133664.0
52	119796.0
53	113053.5
54	109220.0
55	100277.5
56	90059.5
57	78375.5
58	66507.0
59	58422.0
60	47666.5
61	36180.5
62	28170.0
63	21007.0
64	14265.0
65	9803.0
66	7173.0
67	5073.5
68	3489.5
69	2517.5
70	1847.5
71	1406.0
72	1146.0
73	975.5
74	805.0
75	628.0
76	446.5
77	304.5
78	208.5
79	124.0
80	97.0
81	79.0
82	54.0
83	39.5
84	22.5
85	15.5
86	10.5
87	5.0
88	1.5
89	1.5
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002462415945002226
2	0.0040085840965152515
3	2.5769469191883764E-4
4	0.04919105341295145
5	0.0022047212530833886
6	0.004581238967446002
7	0.0
8	2.8632743546537514E-4
9	0.0
10-11	1.5748008950595632E-4
12-13	0.0
14-15	2.8632743546537515E-5
16-17	1.8611283305249385E-4
18-19	1.2884734595941882E-4
20-21	3.006438072386439E-4
22-23	0.0
24-25	0.0
26-27	2.8632743546537514E-4
28-29	0.0
30-31	2.1474557659903134E-4
32-33	2.1474557659903134E-4
34-35	2.4337832014556887E-4
36-37	1.4316371773268757E-5
38-39	8.017168193030504E-4
40-41	5.010730120644064E-4
42-43	0.0
44-45	4.7244026851786893E-4
46-47	0.0
48-49	1.1453097418615006E-4
50-51	0.0
52-53	2.4337832014556887E-4
54-55	3.1496017901191264E-4
56-57	1.8611283305249385E-4
58-59	7.874004475297815E-4
60-61	6.442367297970941E-4
62-63	1.7179646127922507E-4
64-65	2.5769469191883764E-4
66-67	0.001173942485408038
68-69	0.002347884970816076
70-71	0.0033357146231716202
72-73	0.0026771615216012574
74-75	0.002519681432095301
76-77	0.0038797367505558327
78-79	0.0013171062031407256
80-81	9.878296523555443E-4
82-83	7.444513322099754E-4
84-85	5.153893838376753E-4
86-87	0.0
88-89	0.0
90-91	4.294911531980627E-5
92-93	2.8632743546537515E-5
94-95	7.158185886634379E-5
96-97	4.008584096515252E-4
98-99	1.2884734595941882E-4
100	0.07662122173053439
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3492505.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.69308377313577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.20620359304033	34.575827925895474
2	16.107493017344105	16.330769858068486
3	6.678787563801867	10.157050124243565
4	3.398387301418308	6.890989286574365
5	1.8423403553401356	4.669695698594312
6	1.1159563691456407	3.394276182495864
7	0.7080823132832844	2.5126413217881716
8	0.4686722493928193	1.900675328049134
9	0.33594107845763005	1.5326900309781197
>10	1.1135143899365862	8.234621666332693
>50	0.013375731017733813	0.44262838982344926
>100	0.008646528317251233	0.8411217314370929
>500	0.0010062617436257164	0.3550756643955867
>1k	7.546963077191268E-4	0.755848244776355
>5k	2.515654359063756E-4	0.909389882486956
>10k+	5.869860171148763E-4	6.496698664060507
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	68521	1.961944220552297	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	18896	0.5410443220553729	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	16463	0.4713808570066471	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	14683	0.4204145734938103	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	12003	0.3436788207890898	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11421	0.32701456404500495	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	10845	0.3105221037621993	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9873	0.2826910770349649	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	5816	0.1665280364666622	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5704	0.16332116918945	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8632743546537515E-5
2	0.0	0.0	0.0	0.0	2.8632743546537515E-5
3	0.0	0.0	0.0	0.0	2.8632743546537515E-5
4	0.0	0.0	0.0	5.726548709307503E-5	5.726548709307503E-5
5	0.0	0.0	0.0	5.726548709307503E-5	8.589823063961255E-5
6	0.0	0.0	0.0	1.717964612792251E-4	1.1453097418615006E-4
7	0.0	0.0	0.0	1.717964612792251E-4	1.1453097418615006E-4
8	0.0	0.0	0.0	1.717964612792251E-4	1.1453097418615006E-4
9	0.0	0.0	0.0	1.717964612792251E-4	1.1453097418615006E-4
10-11	0.0	0.0	0.0	1.8611283305249385E-4	1.4316371773268757E-4
12-13	0.0	0.0	0.0	2.004292048257626E-4	4.1517478142479394E-4
14-15	0.0	0.0	0.0	2.2906194837230012E-4	4.294911531980627E-4
16-17	0.0	0.0	0.0	4.5812389674460024E-4	4.294911531980627E-4
18-19	0.0	0.0	0.0	6.156039862505566E-4	4.8675664029113774E-4
20-21	0.0	0.0	0.0	7.730840757565129E-4	4.8675664029113774E-4
22-23	0.0	0.0	0.0	0.0012168916007278445	4.8675664029113774E-4
24-25	0.0	0.0	0.0	0.00230493585549627	4.8675664029113774E-4
26-27	0.0	0.0	0.0	0.003593409315090458	5.153893838376753E-4
28-29	0.0	0.0	0.0	0.010150307587247548	5.153893838376753E-4
30-31	0.0	0.0	0.0	0.026886146190198724	5.153893838376753E-4
32-33	0.0	0.0	0.0	0.06608437210540857	5.153893838376753E-4
34-35	0.0	0.0	0.0	0.12229044768726172	5.440221273842128E-4
36-37	2.8632743546537515E-5	0.0	0.0	0.20025740836448336	5.440221273842128E-4
38-39	2.8632743546537515E-5	0.0	0.0	0.32014270559383595	6.156039862505565E-4
40-41	2.8632743546537515E-5	0.0	0.0	0.4527982064449443	6.299203580238253E-4
42-43	2.8632743546537515E-5	0.0	0.0	0.5881308688176539	6.299203580238253E-4
44-45	2.8632743546537515E-5	0.0	0.0	0.762547226131387	7.015022168901691E-4
46-47	5.726548709307503E-5	0.0	0.0	0.9747158558112301	7.730840757565129E-4
48-49	5.726548709307503E-5	0.0	0.0	1.1832910761759825	8.303495628495879E-4
50-51	5.726548709307503E-5	0.0	0.0	1.4123243918047361	8.87615049942663E-4
52-53	5.726548709307503E-5	0.0	0.0	1.6402267140634015	8.87615049942663E-4
54-55	5.726548709307503E-5	0.0	0.0	1.8990523993523274	8.87615049942663E-4
56-57	5.726548709307503E-5	0.0	0.0	2.2423446781035388	9.305641652624692E-4
58-59	5.726548709307503E-5	0.0	0.0	2.5781924435326506	9.735132805822755E-4
60-61	5.726548709307503E-5	0.0	0.0	2.9356865630829447	9.735132805822755E-4
62-63	5.726548709307503E-5	0.0	0.0	3.2914054525333536	0.001002146024128813
64-65	5.726548709307503E-5	0.0	0.0	3.6595223199394127	0.0010307787676753506
66-67	5.726548709307503E-5	0.0	0.0	4.103587539602664	0.0010450951394486193
68-69	5.726548709307503E-5	0.0	0.0	4.611918952156117	0.001059411511221888
70-71	5.726548709307503E-5	0.0	0.0	5.131044909026616	0.0011023606265416943
72-73	5.726548709307503E-5	0.0	0.0	5.634480122433612	0.0012168916007278443
74-75	5.726548709307503E-5	0.0	0.0	6.17870267902265	0.001288473459594188
76-77	5.726548709307503E-5	0.0	0.0	6.733290288775535	0.001288473459594188
78-79	5.726548709307503E-5	0.0	0.0	7.444484689356207	0.001360055318460532
80-81	5.726548709307503E-5	0.0	0.0	8.186745616684872	0.0013743716902338008
82-83	5.726548709307503E-5	0.0	0.0	8.865384587853132	0.0014316371773268758
84-85	5.726548709307503E-5	0.0	0.0	9.547402222759882	0.0014602699208734133
86-87	5.726548709307503E-5	0.0	0.0	10.247730497164643	0.0015175354079664883
88	5.726548709307503E-5	0.0	0.0	10.827414706636068	0.0015748008950595633
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1270	0.0	25.55426	1
GAGTACT	13905	0.0	22.814377	12-13
GTACATG	9480	0.0	22.57465	1
TGATCGC	785	0.0	22.151424	9
TACATGG	9540	0.0	21.742123	2
ACATGGG	9265	0.0	21.523222	3
AGAGTAC	18840	0.0	21.191336	10-11
GTGATCG	840	0.0	20.701035	8
CTGTGCG	1375	0.0	20.507757	9
GTACTTT	15480	0.0	20.356817	14-15
TATATCG	140	5.212213E-4	20.143564	5
GGGCTAT	1970	0.0	20.04074	6
AGTACTT	14875	0.0	20.031254	12-13
TAGTACT	1520	0.0	19.802645	4
CATGGGG	6785	0.0	19.547268	4
CTATTGA	1885	0.0	18.94837	9
GGCGTCG	1105	0.0	18.713715	8
GCTATTG	1865	0.0	18.647581	8
TACGGGC	1180	0.0	18.334229	4
TATTCTC	1520	0.0	18.244062	5
>>END_MODULE