FastQCFastQC Report
Mon 16 Jan 2017
ERR1146520_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146520_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4192457
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61750.032.6731031
GTATCAA95000.032.2770651
CAACGCA97900.029.8676725
ATCAACG100850.029.0406153
AACGCAG107800.027.9026876
TATCAAC109850.027.2604542
GTACATG136250.026.2329521
TCAACGC113650.026.0180594
TACATGG140150.024.8552882
ACGCAGA117300.024.8011747
CGCAGAG117850.024.5253248
ACATGGG143600.022.8831833
GCAGAGT130900.020.9308939
GTACTAG18800.020.763011
CATGGGG97150.020.4203574
CTGTGCG21000.019.2458929
CGTTAAC4200.019.0357361
AGAGTAC101250.018.79864910-11
GTGATCG10450.018.4389768
CTCGTCG10500.017.9031549
Produced by FastQC (version 0.11.5)