##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146520_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4192457
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14403916366942	31.0	31.0	34.0	28.0	34.0
2	31.315845338425653	31.0	31.0	34.0	28.0	34.0
3	31.421121790873467	33.0	31.0	34.0	28.0	34.0
4	34.919728932222796	37.0	35.0	37.0	32.0	37.0
5	34.97447773465536	37.0	35.0	37.0	32.0	37.0
6	35.145135179681034	37.0	35.0	37.0	32.0	37.0
7	35.0949116472751	37.0	35.0	37.0	32.0	37.0
8	35.04645247405042	37.0	35.0	37.0	32.0	37.0
9	36.66681637998911	39.0	37.0	39.0	32.0	39.0
10-11	36.65184854609123	39.0	37.0	39.0	32.0	39.0
12-13	36.59661506367269	39.0	37.0	39.0	32.0	39.0
14-15	37.948277585196465	40.0	38.0	41.0	32.0	41.0
16-17	37.975368501096135	40.0	38.0	41.0	32.0	41.0
18-19	38.00011079421924	40.0	38.0	41.0	32.0	41.0
20-21	38.10508670691196	40.0	38.0	41.0	32.0	41.0
22-23	38.124721136078435	40.0	38.0	41.0	32.0	41.0
24-25	38.149067360738584	40.0	38.0	41.0	32.0	41.0
26-27	38.21483881647444	40.0	38.0	41.0	32.5	41.0
28-29	38.19451362291849	40.0	38.0	41.0	32.5	41.0
30-31	38.15996323874043	40.0	38.0	41.0	32.0	41.0
32-33	38.12634965606087	40.0	38.0	41.0	32.0	41.0
34-35	38.082840921206824	40.0	38.0	41.0	32.0	41.0
36-37	38.00718278088482	40.0	38.0	41.0	32.0	41.0
38-39	37.949919581763154	40.0	38.0	41.0	32.0	41.0
40-41	37.896384864531704	40.0	38.0	41.0	32.0	41.0
42-43	37.74666359130219	40.0	37.5	41.0	31.0	41.0
44-45	37.63881680837752	40.0	37.0	41.0	31.0	41.0
46-47	37.533478578313385	40.0	37.0	41.0	31.0	41.0
48-49	37.4278530465548	40.0	37.0	41.0	31.0	41.0
50-51	37.30262612115044	40.0	36.0	41.0	30.0	41.0
52-53	37.17999624086782	40.0	36.0	41.0	30.0	41.0
54-55	37.05860095881723	40.0	36.0	41.0	30.0	41.0
56-57	36.87654816733958	39.0	35.0	41.0	30.0	41.0
58-59	36.68986145832861	39.0	35.0	41.0	30.0	41.0
60-61	36.47967480644405	39.0	35.0	41.0	30.0	41.0
62-63	36.25509051136362	38.0	35.0	40.5	30.0	41.0
64-65	35.96791284919559	37.5	35.0	40.0	29.0	41.0
66-67	35.73079378035362	37.0	35.0	40.0	29.0	41.0
68-69	35.4778429450797	37.0	34.5	40.0	29.0	41.0
70-71	35.18862304371875	36.0	34.0	39.0	29.0	41.0
72-73	34.84415737597308	36.0	34.0	39.0	29.0	41.0
74-75	34.50519468655254	35.0	34.0	38.5	28.0	40.0
76-77	34.222003469564505	35.0	34.0	37.0	28.0	39.5
78-79	33.897738605309485	35.0	34.0	37.0	28.0	39.0
80-81	33.63982516696056	35.0	34.0	36.5	28.0	39.0
82-83	33.35480936357845	35.0	34.0	36.0	27.0	37.5
84-85	33.135070317954366	35.0	33.5	36.0	27.0	37.0
86-87	32.918474178745306	35.0	33.0	35.0	27.0	37.0
88-89	32.72755117106747	35.0	33.0	35.0	26.0	36.0
90-91	32.567391388868145	35.0	33.0	35.0	26.0	36.0
92-93	32.40963711732762	35.0	33.0	35.0	26.0	36.0
94-95	32.28101039557472	35.0	33.0	35.0	25.0	36.0
96-97	32.17693001979507	35.0	33.0	35.0	25.0	35.0
98-99	32.063883899107374	35.0	33.0	35.0	25.0	35.0
100	30.93470893082505	34.0	31.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15717970209015775
1101	2	0.19904311398536834
1101	3	0.2054985544924648
1101	4	0.19655102299705618
1101	5	0.08307444407974174
1101	6	0.30201558654007954
1101	7	0.2786119417873536
1101	8	0.3281746484173169
1101	9	0.455871477736288
1101	10-11	0.4691724350661488
1101	12-13	0.4167393791451133
1101	14-15	0.5257082041778105
1101	16-17	0.4429476480496888
1101	18-19	0.47721965783879483
1101	20-21	0.47045846943074565
1101	22-23	0.4661416904210043
1101	24-25	0.47676952300579956
1101	26-27	0.4309705767829115
1101	28-29	0.4326951660492
1101	30-31	0.48680049223375477
1101	32-33	0.4811823035066709
1101	34-35	0.45103370556679323
1101	36-37	0.5261067353789102
1101	38-39	0.5071969930296447
1101	40-41	0.5254013497409034
1101	42-43	0.5525440732094609
1101	44-45	0.44712007280531907
1101	46-47	0.47052855067184396
1101	48-49	0.5059745229492236
1101	50-51	0.4401450896753829
1101	52-53	0.4460369656177292
1101	54-55	0.4360129014432914
1101	56-57	0.4787963454835733
1101	58-59	0.5061285012884937
1101	60-61	0.4745281393286618
1101	62-63	0.42249393792027234
1101	64-65	0.5047361402069228
1101	66-67	0.3987953388958232
1101	68-69	0.38833049904381767
1101	70-71	0.36446549636560377
1101	72-73	0.3879466291453184
1101	74-75	0.4016039605787256
1101	76-77	0.36442814817797853
1101	78-79	0.340032318962308
1101	80-81	0.35225011068617107
1101	82-83	0.30663166517812357
1101	84-85	0.2754267281034046
1101	86-87	0.3897129002036692
1101	88-89	0.3174794927076121
1101	90-91	0.3338370466352103
1101	92-93	0.3176495096069303
1101	94-95	0.365088192592971
1101	96-97	0.38718435926298866
1101	98-99	0.3410298848578961
1101	100	0.31255480044779205
1102	1	0.21843460318682162
1102	2	0.2861567492423056
1102	3	0.27591037583016487
1102	4	0.2617066706463902
1102	5	0.23011666131642272
1102	6	0.3264121188229083
1102	7	0.3339083362287525
1102	8	0.35316163822107427
1102	9	0.43431052894545275
1102	10-11	0.38130828218974955
1102	12-13	0.37331545186401627
1102	14-15	0.4586981445009357
1102	16-17	0.4676321660929901
1102	18-19	0.39380093871675825
1102	20-21	0.3834522890094476
1102	22-23	0.4453030353529286
1102	24-25	0.5401166989703015
1102	26-27	0.4380223003720758
1102	28-29	0.4531373263756251
1102	30-31	0.5130115150324102
1102	32-33	0.4642422526954917
1102	34-35	0.4685257545890451
1102	36-37	0.49227774281288106
1102	38-39	0.48679557032762233
1102	40-41	0.44863819951333284
1102	42-43	0.45093416853958956
1102	44-45	0.4136227960372949
1102	46-47	0.44762972116784994
1102	48-49	0.48738163994142525
1102	50-51	0.4798364236197159
1102	52-53	0.41297256861482623
1102	54-55	0.4452402114026057
1102	56-57	0.41209028777841894
1102	58-59	0.4180819472290551
1102	60-61	0.377646655483467
1102	62-63	0.37816735601506934
1102	64-65	0.47956032233280155
1102	66-67	0.40496559278575006
1102	68-69	0.31777800028363146
1102	70-71	0.30099930396921337
1102	72-73	0.3405051831895278
1102	74-75	0.3192896799398852
1102	76-77	0.31700282044752726
1102	78-79	0.2874381075951078
1102	80-81	0.25473909792570737
1102	82-83	0.2793194343422911
1102	84-85	0.29768548723672694
1102	86-87	0.26828580749354103
1102	88-89	0.28560883051650876
1102	90-91	0.30906132800039643
1102	92-93	0.29261889177971057
1102	94-95	0.2752918249472671
1102	96-97	0.20416884740935615
1102	98-99	0.2131075893941059
1102	100	0.18748981747598137
1103	1	0.15573128582030193
1103	2	0.2010334190281604
1103	3	0.20810239033448497
1103	4	0.2240296739930514
1103	5	0.23088201897028426
1103	6	0.25537678734231406
1103	7	0.31856714761041616
1103	8	0.2701560890386787
1103	9	0.36208486914060245
1103	10-11	0.37980743480580514
1103	12-13	0.3948892647545392
1103	14-15	0.49437594777764815
1103	16-17	0.4721066084010701
1103	18-19	0.46288781440374294
1103	20-21	0.4222627354831374
1103	22-23	0.40268679656058737
1103	24-25	0.5012107919600766
1103	26-27	0.42759830025133994
1103	28-29	0.4457621119406596
1103	30-31	0.5030600658792608
1103	32-33	0.4852432550074468
1103	34-35	0.516654235536123
1103	36-37	0.4855426522900217
1103	38-39	0.4965051082417986
1103	40-41	0.4139961438474842
1103	42-43	0.44763270527022314
1103	44-45	0.4468956157783168
1103	46-47	0.4274228867428036
1103	48-49	0.4237776752239668
1103	50-51	0.4841337895529918
1103	52-53	0.4422564734600414
1103	54-55	0.35654785373289855
1103	56-57	0.4103800334129488
1103	58-59	0.4426844496811597
1103	60-61	0.37790732100017266
1103	62-63	0.37192203945390645
1103	64-65	0.35992190670176427
1103	66-67	0.38275943869265205
1103	68-69	0.3627554310574581
1103	70-71	0.39394441512367706
1103	72-73	0.4008200296251445
1103	74-75	0.46839796455050475
1103	76-77	0.39424846997605556
1103	78-79	0.39277087699510105
1103	80-81	0.380316210306205
1103	82-83	0.43675127154102
1103	84-85	0.3435261041653206
1103	86-87	0.331760449784106
1103	88-89	0.22704158913752082
1103	90-91	0.2983166581460921
1103	92-93	0.31292633311718276
1103	94-95	0.284052377464711
1103	96-97	0.34851483996435206
1103	98-99	0.3330218255137396
1103	100	0.37627568132232625
1104	1	0.19645639535862713
1104	2	0.20291389913078106
1104	3	0.15545669697245046
1104	4	0.2313523503887538
1104	5	0.2105499524101404
1104	6	0.32149235498891926
1104	7	0.3705396531599874
1104	8	0.36596411324978106
1104	9	0.4185204814080805
1104	10-11	0.44258153553110446
1104	12-13	0.45005997323322333
1104	14-15	0.5949209627515373
1104	16-17	0.5541521123079391
1104	18-19	0.5360452406278853
1104	20-21	0.5345180778025025
1104	22-23	0.5213727037683427
1104	24-25	0.5487105039025835
1104	26-27	0.5478787713808444
1104	28-29	0.5479013849345407
1104	30-31	0.5356677662423692
1104	32-33	0.5529881574094944
1104	34-35	0.5021495975555652
1104	36-37	0.5379046727507699
1104	38-39	0.5540428845603103
1104	40-41	0.5260367431294242
1104	42-43	0.5127571512056477
1104	44-45	0.5378677409900234
1104	46-47	0.5037976857398476
1104	48-49	0.47265985919940334
1104	50-51	0.5116891144359599
1104	52-53	0.4229460168200845
1104	54-55	0.45674740364944455
1104	56-57	0.4712476523857916
1104	58-59	0.43763166633200257
1104	60-61	0.44192674780145325
1104	62-63	0.4241061270560209
1104	64-65	0.5064922890891594
1104	66-67	0.4393240395367357
1104	68-69	0.419077247869204
1104	70-71	0.41236551337217975
1104	72-73	0.43616432715743514
1104	74-75	0.4481925413201253
1104	76-77	0.41267690986686034
1104	78-79	0.41750436008433667
1104	80-81	0.37301484506663485
1104	82-83	0.4311577558426478
1104	84-85	0.4494355144173099
1104	86-87	0.4308292848184223
1104	88-89	0.40876752766962454
1104	90-91	0.43982416242616296
1104	92-93	0.378022126254173
1104	94-95	0.43340187736631464
1104	96-97	0.3889701441479687
1104	98-99	0.4112089904875873
1104	100	0.5019723470950801
1105	1	0.12078237639626721
1105	2	0.022255406911803277
1105	3	0.07245531355911794
1105	4	0.13717734902925827
1105	5	0.12673039346816495
1105	6	0.23668803580110165
1105	7	0.2510756055357177
1105	8	0.28508280578609657
1105	9	0.36951769778342936
1105	10-11	0.35761635432787386
1105	12-13	0.35652360831048213
1105	14-15	0.40737230910966815
1105	16-17	0.32295884641897743
1105	18-19	0.35484884291207663
1105	20-21	0.37247248808377975
1105	22-23	0.36046503486394244
1105	24-25	0.3783643060684909
1105	26-27	0.33563681611870777
1105	28-29	0.32497568638768115
1105	30-31	0.39695030058498304
1105	32-33	0.36026054406369923
1105	34-35	0.3013545975455969
1105	36-37	0.26091101583898535
1105	38-39	0.3142555726158349
1105	40-41	0.3026705104295644
1105	42-43	0.3017434841979991
1105	44-45	0.25997077980161265
1105	46-47	0.23840374605256898
1105	48-49	0.31970695931280346
1105	50-51	0.3213326356684689
1105	52-53	0.2880688826580311
1105	54-55	0.26049555978637784
1105	56-57	0.2813876108805502
1105	58-59	0.2496281148403625
1105	60-61	0.25942799412617745
1105	62-63	0.2882400616241796
1105	64-65	0.2752690573114833
1105	66-67	0.2533218392860235
1105	68-69	0.2064473589095499
1105	70-71	0.24729241510997468
1105	72-73	0.2023875745942476
1105	74-75	0.23897287014485613
1105	76-77	0.19568183793877125
1105	78-79	0.2118444656610734
1105	80-81	0.08685697878595988
1105	82-83	0.0983879425536216
1105	84-85	0.15272685090164373
1105	86-87	0.10070989388471929
1105	88-89	0.12215749676987997
1105	90-91	0.09868276243692975
1105	92-93	0.0884732317463488
1105	94-95	0.16761979044786557
1105	96-97	0.17352592440418846
1105	98-99	0.14481196936739593
1105	100	0.2879612086105361
1106	1	0.07167068836717405
1106	2	0.06601415833854674
1106	3	0.04705324009818668
1106	4	0.06405142247614748
1106	5	0.12058093788134983
1106	6	0.16858261913201034
1106	7	0.20670195790430768
1106	8	0.2364636160013731
1106	9	0.27045848404117123
1106	10-11	0.2892376535032497
1106	12-13	0.2625088411501224
1106	14-15	0.3309423231009845
1106	16-17	0.31847676143333814
1106	18-19	0.3068546669053376
1106	20-21	0.2617704699761205
1106	22-23	0.25792197595246336
1106	24-25	0.30751255774633535
1106	26-27	0.33125941648530954
1106	28-29	0.3001889478337745
1106	30-31	0.26217394172908115
1106	32-33	0.28671346323859126
1106	34-35	0.29565758119329644
1106	36-37	0.3127199008560808
1106	38-39	0.26221716400428363
1106	40-41	0.2575457220897448
1106	42-43	0.27178049641990754
1106	44-45	0.20656693749274524
1106	46-47	0.2551257367435227
1106	48-49	0.23193887515126477
1106	50-51	0.22150997013262952
1106	52-53	0.1935320322624463
1106	54-55	0.18122489369689987
1106	56-57	0.17879733662686448
1106	58-59	0.17539676799886905
1106	60-61	0.21758886716143166
1106	62-63	0.21105838652913178
1106	64-65	0.2561715755617726
1106	66-67	0.2217791117045067
1106	68-69	0.15765655424276304
1106	70-71	0.18196456021868102
1106	72-73	0.16064677179743114
1106	74-75	0.20812674708379575
1106	76-77	0.2333145769364151
1106	78-79	0.21129891184923366
1106	80-81	0.14191799186598075
1106	82-83	0.17661608730653455
1106	84-85	0.22073347728154147
1106	86-87	0.1890760664665052
1106	88-89	0.17872907177388697
1106	90-91	0.258479304343588
1106	92-93	0.1785828897899151
1106	94-95	0.12700412212703327
1106	96-97	0.14828685629136373
1106	98-99	0.18878837200579568
1106	100	0.2159787049383226
1107	1	0.12167121002020309
1107	2	0.1401078150324473
1107	3	0.18099192861842894
1107	4	0.13600606581987762
1107	5	0.0998187802594046
1107	6	0.12267159510404468
1107	7	0.16374144536106883
1107	8	0.2039593370047612
1107	9	0.2769438632601151
1107	10-11	0.2163848101455912
1107	12-13	0.19451402926475225
1107	14-15	0.21924095619703365
1107	16-17	0.24623151278807853
1107	18-19	0.2676470547136347
1107	20-21	0.32611428734701775
1107	22-23	0.36261830284929886
1107	24-25	0.35929093322023675
1107	26-27	0.28763818712579337
1107	28-29	0.2835078601258729
1107	30-31	0.2973145214538917
1107	32-33	0.3127355610901148
1107	34-35	0.25851152080873163
1107	36-37	0.3371399209871768
1107	38-39	0.32840051607954734
1107	40-41	0.2826591999735797
1107	42-43	0.31639970831909636
1107	44-45	0.3030736656896522
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2216	64-65	-0.4506756827472671
2216	66-67	-0.4162771433099337
2216	68-69	-0.4870032462391407
2216	70-71	-0.47490127427979445
2216	72-73	-0.39396239387319554
2216	74-75	-0.3937942096600082
2216	76-77	-0.43135057980089186
2216	78-79	-0.38473604198486555
2216	80-81	-0.38562573827150004
2216	82-83	-0.3591949932495382
2216	84-85	-0.33435949868037085
2216	86-87	-0.3701827025094744
2216	88-89	-0.3013035376114459
2216	90-91	-0.32058017682432194
2216	92-93	-0.29984590277226886
2216	94-95	-0.3782761416017095
2216	96-97	-0.31386550646787015
2216	98-99	-0.3125215835111952
2216	100	-0.34164653497392905
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1256.0
1	312.0
2	230.0
3	200.0
4	148.0
5	227.0
6	303.0
7	745.0
8	2336.0
9	3024.0
10	2953.0
11	3679.0
12	4592.0
13	6346.0
14	9056.0
15	10537.0
16	11251.0
17	12352.0
18	13820.0
19	15642.0
20	17408.0
21	19507.0
22	21714.0
23	24835.0
24	29770.0
25	36339.0
26	40626.0
27	39003.0
28	41011.0
29	51152.0
30	60083.0
31	67801.0
32	85857.0
33	114679.0
34	169273.0
35	256985.0
36	465124.0
37	959163.0
38	1203516.0
39	389055.0
40	547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.506640498625345	20.16562746593797	13.255712619514053	24.072019415922632
2	14.877856674025896	21.06889432543708	40.335270600871176	23.717978399665853
3	17.752178622973737	26.616726114400326	30.634114994244605	24.996980268381332
4	11.899507156319416	17.918932048685175	40.04165716747971	30.13990362751569
5	12.806844322132708	38.921543725226385	35.64741561810982	12.624196334531094
6	29.43604587036375	39.1500747014449	18.59177868700214	12.822100741189216
7	26.057641462701355	33.56955709536684	23.290302173043752	17.082499268888053
8	25.163729721638582	35.716702043061396	21.139152537395887	17.98041569790413
9	26.141410736957738	15.820264434110957	20.862634473345697	37.17569035558561
10-11	23.9718709727627	26.788032189132938	29.27820091101725	19.96189592708711
12-13	24.66300547984573	24.881347185629714	28.5004847794528	21.95516255507176
14-15	22.181492621824667	26.23186518587144	25.926361354600836	25.66028083770306
16-17	21.853146540143257	28.84525108118099	26.853416887609576	22.44818549106618
18-19	21.792091208734153	28.761092103323588	27.970821703372284	21.475994984569972
20-21	23.046618124271237	27.78489616215913	27.24096036128718	21.92752535228245
22-23	22.984512245846688	27.747147330297405	26.918615441445453	22.34972498241045
24-25	22.49435657696748	28.00130861291447	27.23101255058134	22.273322259536705
26-27	22.469762536420237	28.01633192412346	27.011728351374824	22.502177188081482
28-29	22.230727134163626	28.293808966521905	27.143592898922375	22.33187100039209
30-31	22.593704914698478	27.99345513757735	26.995623827491215	22.41721612023296
32-33	22.48840145088694	28.260449198187775	26.644088667010752	22.60706068391453
34-35	22.771128589311406	28.113121895944005	27.058075591817033	22.05767392292756
36-37	22.750802023117938	27.84604211916495	27.043016323122092	22.36013953459502
38-39	22.576423029552682	27.978097082260916	27.074356426023282	22.37112346216312
40-41	22.61285911182299	27.7158705382534	27.30882133700778	22.36244901291583
42-43	22.462593102434045	27.788883143225075	27.074550704209884	22.673973050130996
44-45	22.442483229559663	28.040794553437003	27.13854074519614	22.378181471807196
46-47	22.454515277831987	27.98318867337406	26.95986821554698	22.602427833246974
48-49	22.523355761772304	27.76670667225965	27.123362303367177	22.586575262600867
50-51	22.366721934344863	27.8529999128609	26.955061289825856	22.825216862968386
52-53	22.31030772253712	28.036858708443468	26.874921958581165	22.77791161043825
54-55	22.349354180237004	27.8527968533408	26.90754337106827	22.890305595353922
56-57	22.52776571440713	27.792430230278935	26.839732994566827	22.840071060747103
58-59	22.34085948633	27.58498841011515	27.217779409817776	22.85637269373707
60-61	22.30231913224112	27.807251387373956	27.067040063164953	22.82338941721997
62-63	22.358911927081703	27.61432341401489	27.05282665597672	22.973938002926683
64-65	22.048740399081147	27.659707311203118	27.04102719236649	23.25052509734924
66-67	22.300565410433823	27.64175463114016	26.920477585041674	23.137202373384344
68-69	22.17351281136959	27.548864637114956	27.080420229492397	23.19720232202306
70-71	22.210413995987324	27.322001733907097	27.125952281978847	23.341631988126725
72-73	22.399462509166796	27.319609473944457	27.272717505375923	23.008210511512825
74-75	22.303181623694897	27.64249878706148	27.135320960723714	22.91899862851991
76-77	22.258901022393037	27.419107606861093	27.136237017738175	23.18575435300769
78-79	22.285879928099746	27.454152602754906	27.136353564353975	23.123613904791377
80-81	22.353977836198172	27.39437024104839	27.316986633121832	22.93466528963161
82-83	22.50714832338239	27.061540647247107	27.622327362767113	22.808983666603392
84-85	22.571269723459398	27.27703592350063	27.167036177787	22.98465817525297
86-87	22.229224473540864	27.442572353623536	27.32813548484834	23.00006768798727
88-89	22.577609884862515	26.945010295140083	27.194653116810684	23.282726703186718
90-91	22.493430042095003	26.89182197521096	27.419627097139877	23.195120885554164
92-93	22.727393184614744	26.892740789126247	27.30253341106847	23.077332615190535
94-95	22.22903304976947	26.85120680171284	27.52786616600519	23.391893982512503
96-97	22.35162109349354	26.81975369358164	27.471652797954487	23.356972414970333
98-99	22.266275464325947	27.03699087465146	27.233499374658855	23.463234286363743
100	22.61563343916437	26.717984915849264	26.979932273875228	23.68644937111114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2408.0
1	1614.0
2	853.5
3	930.0
4	915.5
5	840.5
6	769.5
7	758.5
8	796.5
9	784.5
10	737.0
11	656.0
12	656.5
13	707.5
14	752.5
15	858.0
16	988.5
17	1212.0
18	1468.5
19	1764.0
20	2348.0
21	3272.5
22	4952.5
23	7208.5
24	10103.5
25	14406.5
26	19760.0
27	26583.0
28	33968.0
29	41717.5
30	51188.0
31	61235.0
32	73333.0
33	84706.0
34	92810.5
35	100641.0
36	109098.5
37	120761.5
38	126856.0
39	126393.5
40	127622.5
41	129818.5
42	133263.5
43	138430.5
44	144581.0
45	155730.5
46	167181.0
47	172520.0
48	174640.5
49	169697.5
50	160894.5
51	158886.5
52	155949.5
53	148808.5
54	141573.5
55	128093.5
56	115928.5
57	103053.0
58	89025.5
59	78956.0
60	65044.0
61	50412.5
62	39759.5
63	30520.5
64	21794.0
65	15257.0
66	10993.0
67	7924.0
68	5715.5
69	4282.0
70	3477.0
71	2628.0
72	2045.5
73	1781.0
74	1396.0
75	1106.5
76	836.0
77	571.5
78	422.0
79	287.5
80	205.5
81	155.5
82	115.5
83	97.5
84	79.5
85	58.5
86	41.5
87	35.5
88	28.5
89	19.0
90	13.0
91	10.0
92	10.0
93	8.5
94	7.5
95	8.5
96	8.0
97	9.5
98	10.0
99	6.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09879648139503876
2	0.06456834262104537
3	0.07973844454457135
4	0.08353097002545286
5	0.08407957434029735
6	0.09023348361116167
7	0.08663177702239999
8	0.08811062343632863
9	0.07294052151280263
10-11	0.08118151241622752
12-13	0.06972045270828062
14-15	0.07898709515684954
16-17	0.08118151241622752
18-19	0.08072831754744295
20-21	0.07136626565281409
22-23	0.07583858343687246
24-25	0.07843849084200506
26-27	0.07140204419508656
28-29	0.08048979393229316
30-31	0.0840199434365099
32-33	0.08517678296998633
34-35	0.08786017364042135
36-37	0.08765742856754404
38-39	0.08825373760541849
40-41	0.08416305760559978
42-43	0.09042430250328148
44-45	0.0861905083343729
46-47	0.08337592967560549
48-49	0.09245175323205462
50-51	0.08949406040419734
52-53	0.09483698938355242
54-55	0.08666755556467245
56-57	0.09369207603083347
58-59	0.09794972256125704
60-61	0.0968048092085381
62-63	0.09774697748837972
64-65	0.09984598530169779
66-67	0.10162298623456366
68-69	0.09842676979155658
70-71	0.10084778448532686
72-73	0.09794972256125704
74-75	0.1021119596456207
76-77	0.09889189084109867
78-79	0.10573751859589735
80-81	0.10093126775062929
82-83	0.10523661900408281
84-85	0.10174224804213855
86-87	0.09830750798398172
88-89	0.09715066845050528
90-91	0.09717452081202026
92-93	0.09242790087053963
94-95	0.09218937725538986
96-97	0.08997110763449691
98-99	0.10359080605954933
100	0.24613251847305767
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4192457.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.09743409661072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.69806860706234	36.47691170427934
2	15.288709181388393	16.235824563620348
3	6.363957784728015	10.137294872046255
4	3.233075271926901	6.866720047220816
5	1.9477961079811135	5.1711487738581
6	1.2317780220032382	3.924255140698226
7	0.784683922850498	2.9165292016157003
8	0.5311739476957379	2.2563178945288795
9	0.39205563289159745	1.8735433316699943
>10	1.4934275737407434	12.455133156530227
>50	0.02746827430514885	0.9656243917993869
>100	0.0077254109472525475	0.623998230039679
>500	0.0	0.0
>1k	8.026247905525739E-5	0.09669869209300311
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.3852361514977972E-5	0.0
3	0.0	0.0	0.0	2.3852361514977972E-5	0.0
4	0.0	0.0	0.0	4.7704723029955944E-5	0.0
5	0.0	0.0	0.0	4.7704723029955944E-5	0.0
6	0.0	0.0	0.0	4.7704723029955944E-5	0.0
7	0.0	0.0	0.0	7.155708454493391E-5	0.0
8	0.0	0.0	0.0	7.155708454493391E-5	0.0
9	0.0	0.0	0.0	1.669665306048458E-4	0.0
10-11	0.0	0.0	0.0	2.1467125363480174E-4	0.0
12-13	0.0	1.1926180757488986E-5	0.0	3.6971160348215856E-4	0.0
14-15	0.0	2.3852361514977972E-5	0.0	3.8163778423964755E-4	0.0
16-17	0.0	2.3852361514977972E-5	0.0	5.247519533295153E-4	0.0
18-19	0.0	2.3852361514977972E-5	0.0	6.559399416618942E-4	0.0
20-21	0.0	2.3852361514977972E-5	0.0	7.632755684792951E-4	0.0
22-23	0.0	2.3852361514977972E-5	0.0	0.0011210609912039645	0.0
24-25	0.0	2.3852361514977972E-5	0.0	0.0018008532943808368	0.0
26-27	0.0	2.3852361514977972E-5	0.0	0.0028145786587674007	0.0
28-29	0.0	2.3852361514977972E-5	0.0	0.0074419367926731265	0.0
30-31	0.0	2.3852361514977972E-5	0.0	0.01926078192334471	0.0
32-33	0.0	2.3852361514977972E-5	0.0	0.04622587661602731	1.1926180757488986E-5
34-35	0.0	2.3852361514977972E-5	0.0	0.08614280361134294	2.3852361514977972E-5
36-37	0.0	2.3852361514977972E-5	0.0	0.15099737457056805	2.3852361514977972E-5
38-39	1.1926180757488986E-5	2.3852361514977972E-5	0.0	0.2501993461113614	2.3852361514977972E-5
40-41	2.3852361514977972E-5	2.3852361514977972E-5	0.0	0.36263937829296755	2.3852361514977972E-5
42-43	2.3852361514977972E-5	2.3852361514977972E-5	0.0	0.4722290532735339	2.3852361514977972E-5
44-45	2.3852361514977972E-5	2.3852361514977972E-5	0.0	0.611157132917523	2.3852361514977972E-5
46-47	2.3852361514977972E-5	2.3852361514977972E-5	0.0	0.7807832018312889	2.3852361514977972E-5
48-49	2.3852361514977972E-5	2.3852361514977972E-5	0.0	0.9692049316188573	2.3852361514977972E-5
50-51	2.3852361514977972E-5	2.3852361514977972E-5	0.0	1.1741802002978208	2.3852361514977972E-5
52-53	2.3852361514977972E-5	2.3852361514977972E-5	0.0	1.3698769003474573	2.3852361514977972E-5
54-55	2.3852361514977972E-5	2.3852361514977972E-5	0.0	1.5822941058190936	2.3852361514977972E-5
56-57	2.3852361514977972E-5	2.3852361514977972E-5	0.0	1.8399234625423708	2.3852361514977972E-5
58-59	2.3852361514977972E-5	2.3852361514977972E-5	0.0	2.1358358594971874	2.3852361514977972E-5
60-61	2.3852361514977972E-5	2.3852361514977972E-5	0.0	2.450794367121714	2.3852361514977972E-5
62-63	2.3852361514977972E-5	3.5778542272466956E-5	0.0	2.764464847224432	2.3852361514977972E-5
64-65	2.3852361514977972E-5	4.7704723029955944E-5	0.0	3.0827388330995404	2.3852361514977972E-5
66-67	2.3852361514977972E-5	4.7704723029955944E-5	0.0	3.4494808175730842	2.3852361514977972E-5
68-69	2.3852361514977972E-5	4.7704723029955944E-5	0.0	3.864142196330219	2.3852361514977972E-5
70-71	2.3852361514977972E-5	4.7704723029955944E-5	0.0	4.312876673511499	2.3852361514977972E-5
72-73	2.3852361514977972E-5	4.7704723029955944E-5	0.0	4.778057353957357	2.3852361514977972E-5
74-75	2.3852361514977972E-5	4.7704723029955944E-5	0.0	5.229212845832408	2.3852361514977972E-5
76-77	2.3852361514977972E-5	4.7704723029955944E-5	0.0	5.721978782370337	2.3852361514977972E-5
78-79	2.3852361514977972E-5	4.7704723029955944E-5	0.0	6.273910501646171	4.7704723029955944E-5
80-81	2.3852361514977972E-5	4.7704723029955944E-5	0.0	6.875216609258008	4.7704723029955944E-5
82-83	2.3852361514977972E-5	4.7704723029955944E-5	0.0	7.497166935761059	4.7704723029955944E-5
84-85	2.3852361514977972E-5	4.7704723029955944E-5	0.0	8.106225060865263	4.7704723029955944E-5
86-87	2.3852361514977972E-5	4.7704723029955944E-5	0.0	8.727841454307104	4.7704723029955944E-5
88	2.3852361514977972E-5	4.7704723029955944E-5	0.0	9.227786951661043	4.7704723029955944E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6175	0.0	32.673103	1
GTATCAA	9500	0.0	32.277065	1
CAACGCA	9790	0.0	29.867672	5
ATCAACG	10085	0.0	29.040615	3
AACGCAG	10780	0.0	27.902687	6
TATCAAC	10985	0.0	27.260454	2
GTACATG	13625	0.0	26.232952	1
TCAACGC	11365	0.0	26.018059	4
TACATGG	14015	0.0	24.855288	2
ACGCAGA	11730	0.0	24.801174	7
CGCAGAG	11785	0.0	24.525324	8
ACATGGG	14360	0.0	22.883183	3
GCAGAGT	13090	0.0	20.930893	9
GTACTAG	1880	0.0	20.76301	1
CATGGGG	9715	0.0	20.420357	4
CTGTGCG	2100	0.0	19.245892	9
CGTTAAC	420	0.0	19.035736	1
AGAGTAC	10125	0.0	18.798649	10-11
GTGATCG	1045	0.0	18.438976	8
CTCGTCG	1050	0.0	17.903154	9
>>END_MODULE