FastQCFastQC Report
Mon 16 Jan 2017
ERR1146520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4192457
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149880.3574991943864898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133000.317236408149207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77440.18471268757198941No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102950.050.9778631
GTATCAA179350.039.4619871
ATCAACG217200.032.063283
CAACGCA218700.031.8485265
TATCAAC225050.031.1762032
AACGCAG232800.030.262046
TCAACGC233350.029.9039424
ACGCAGA256250.027.1985977
CGCAGAG257950.026.9829068
GTACATG132850.026.0885371
TACATGG135350.024.6332342
GCAGAGT281800.024.3156419
ACATGGG134800.023.9651173
GAGTACT190100.023.0781712-13
AGAGTAC245600.021.8719310-11
CATGGGG96750.021.6763534
CAGAGTA269750.021.66474310-11
GTACTTT205050.021.01751314-15
AGTACTT198400.020.44270512-13
GTACTAG18250.018.810651
Produced by FastQC (version 0.11.5)