##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146520_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4192457 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28920940632188 33.0 31.0 34.0 30.0 34.0 2 32.38615899936481 34.0 31.0 34.0 30.0 34.0 3 32.5318225088534 34.0 31.0 34.0 31.0 34.0 4 36.05962183989007 37.0 35.0 37.0 35.0 37.0 5 36.08449794476127 37.0 35.0 37.0 35.0 37.0 6 36.15367504067424 37.0 36.0 37.0 35.0 37.0 7 36.12759987758968 37.0 36.0 37.0 35.0 37.0 8 36.129218260318474 37.0 36.0 37.0 35.0 37.0 9 37.91605352183696 39.0 38.0 39.0 35.0 39.0 10-11 37.867584330620446 39.0 38.0 39.0 35.0 39.0 12-13 37.834303249860405 39.0 38.0 39.0 35.0 39.0 14-15 39.191594332392675 40.0 39.0 41.0 36.0 41.0 16-17 39.13959928509702 40.0 39.0 41.0 36.0 41.0 18-19 39.25262692020455 40.5 39.0 41.0 36.0 41.0 20-21 39.31139186400719 41.0 39.0 41.0 36.0 41.0 22-23 39.27153230194132 41.0 39.0 41.0 36.0 41.0 24-25 39.25783412924689 41.0 39.0 41.0 36.0 41.0 26-27 39.17810904202476 41.0 39.0 41.0 35.5 41.0 28-29 39.11507774557974 40.5 39.0 41.0 35.0 41.0 30-31 38.962308379072226 40.0 38.5 41.0 35.0 41.0 32-33 38.844400431536926 40.0 38.0 41.0 35.0 41.0 34-35 38.762555346423355 40.0 38.0 41.0 35.0 41.0 36-37 38.67904584352326 40.0 38.0 41.0 35.0 41.0 38-39 38.583956019107646 40.0 38.0 41.0 35.0 41.0 40-41 38.47975685856766 40.0 38.0 41.0 34.5 41.0 42-43 38.362069903161796 40.0 38.0 41.0 34.0 41.0 44-45 38.20328854893443 40.0 38.0 41.0 34.0 41.0 46-47 37.94581781041523 40.0 38.0 41.0 33.0 41.0 48-49 37.64825089440393 40.0 37.0 41.0 33.0 41.0 50-51 37.53130562817937 40.0 37.0 41.0 33.0 41.0 52-53 37.430671203067796 40.0 37.0 41.0 32.0 41.0 54-55 37.265792111880934 40.0 36.0 41.0 32.0 41.0 56-57 37.11086279477643 40.0 36.0 41.0 32.0 41.0 58-59 36.93690096284828 39.0 35.5 41.0 31.5 41.0 60-61 36.737943883503156 39.0 35.0 41.0 31.0 41.0 62-63 36.501361015748046 39.0 35.0 41.0 31.0 41.0 64-65 36.2324561706894 38.0 35.0 40.5 31.0 41.0 66-67 35.966309135669135 37.0 35.0 40.0 31.0 41.0 68-69 35.6792023150148 37.0 35.0 40.0 30.5 41.0 70-71 35.3679346025493 36.5 35.0 39.0 30.0 41.0 72-73 35.04380521970768 36.0 35.0 39.0 30.0 41.0 74-75 34.7235070031726 35.5 35.0 39.0 30.0 40.5 76-77 34.42534974121381 35.0 34.0 37.0 29.5 39.5 78-79 34.1407121170235 35.0 34.0 37.0 29.0 39.0 80-81 33.877105358504565 35.0 34.0 36.5 29.0 39.0 82-83 33.62125932358997 35.0 34.0 36.0 29.0 37.5 84-85 33.40954743244832 35.0 34.0 36.0 29.0 37.0 86-87 33.22154431160534 35.0 34.0 35.5 29.0 37.0 88-89 33.02645143408746 35.0 34.0 35.0 29.0 36.0 90-91 32.93982108820675 35.0 34.0 35.0 29.0 36.0 92-93 32.84361807407923 35.0 34.0 35.0 29.0 36.0 94-95 32.76907932508312 35.0 34.0 35.0 29.0 36.0 96-97 32.71443332632869 35.0 34.0 35.0 29.0 35.5 98-99 32.647752022262836 35.0 34.0 35.0 28.0 35.0 100 31.92461628109722 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2539297648250951 1101 2 0.3050119190652012 1101 3 0.28768734189112166 1101 4 0.22154977472575865 1101 5 0.2124204158999916 1101 6 0.21428963341872986 1101 7 0.2105606588867701 1101 8 0.21642951737244687 1101 9 0.24689547454799765 1101 10-11 0.26030745756557394 1101 12-13 0.2974724661277648 1101 14-15 0.41718580269093053 1101 16-17 0.4218799410735343 1101 18-19 0.38974927346062316 1101 20-21 0.38105701386471225 1101 22-23 0.4127942118669239 1101 24-25 0.3963087119457853 1101 26-27 0.36810566987201554 1101 28-29 0.3902538141724534 1101 30-31 0.4495919031767741 1101 32-33 0.38325468778163696 1101 34-35 0.36881590132786357 1101 36-37 0.344529461728996 1101 38-39 0.38877863401724966 1101 40-41 0.4254808476259626 1101 42-43 0.4240609091180261 1101 44-45 0.4180124239729466 1101 46-47 0.44618945443316704 1101 48-49 0.4797535046708248 1101 50-51 0.47519338666603517 1101 52-53 0.4363921245834419 1101 54-55 0.38526308022288447 1101 56-57 0.3420044386809735 1101 58-59 0.2974985577582814 1101 60-61 0.29816798725033067 1101 62-63 0.3256121990367262 1101 64-65 0.275501124217854 1101 66-67 0.25047979753250615 1101 68-69 0.19101136064904267 1101 70-71 0.21006750202564461 1101 72-73 0.27930636605659487 1101 74-75 0.2718416053108399 1101 76-77 0.2786468444994554 1101 78-79 0.21587676847948956 1101 80-81 0.13711643858106726 1101 82-83 0.20293989858105732 1101 84-85 0.25148706171572144 1101 86-87 0.2095857846253395 1101 88-89 0.3265698477898695 1101 90-91 0.24403819865464982 1101 92-93 0.23971183459607204 1101 94-95 0.2312883127905181 1101 96-97 0.26869449222770925 1101 98-99 0.27092725873437473 1101 100 0.18502906513115036 1102 1 0.19936875591396586 1102 2 0.20845776441239394 1102 3 0.20834071897897388 1102 4 0.16622624453788148 1102 5 0.14517579183821283 1102 6 0.16391256215876382 1102 7 0.1713290344117695 1102 8 0.1918076699817064 1102 9 0.23645596522758439 1102 10-11 0.27344483136394615 1102 12-13 0.2662985941382985 1102 14-15 0.3846117193826899 1102 16-17 0.4180224824973777 1102 18-19 0.37179673286105697 1102 20-21 0.33837109047539116 1102 22-23 0.40476730163958763 1102 24-25 0.37294048163444415 1102 26-27 0.32678265465123246 1102 28-29 0.3075000568576982 1102 30-31 0.3744064007655723 1102 32-33 0.3627164301876391 1102 34-35 0.3971292983887196 1102 36-37 0.38314753949528324 1102 38-39 0.3834759419063687 1102 40-41 0.34033145956085065 1102 42-43 0.3798183188090434 1102 44-45 0.3558403142427906 1102 46-47 0.4375631094454704 1102 48-49 0.5126212525235658 1102 50-51 0.4742628141874832 1102 52-53 0.4422377016471941 1102 54-55 0.4055612306667342 1102 56-57 0.3205549912844674 1102 58-59 0.31933582869925914 1102 60-61 0.27774605971733024 1102 62-63 0.31586784769260134 1102 64-65 0.2931350068222258 1102 66-67 0.2744871800877746 1102 68-69 0.2629721638453759 1102 70-71 0.33563027972498105 1102 72-73 0.28435143859948653 1102 74-75 0.3794661243824393 1102 76-77 0.3419044520977863 1102 78-79 0.2956565431943048 1102 80-81 0.2277433368931554 1102 82-83 0.246472538871501 1102 84-85 0.2688846456957492 1102 86-87 0.28272774089638375 1102 88-89 0.36228113623320723 1102 90-91 0.3359016096846261 1102 92-93 0.37279961322713717 1102 94-95 0.34570975827296735 1102 96-97 0.2863570407898166 1102 98-99 0.3411829985228252 1102 100 0.293452558512346 1103 1 0.20603703826902375 1103 2 0.19913830916038222 1103 3 0.22526727552246228 1103 4 0.15516374650724174 1103 5 0.10755155343080247 1103 6 0.1491814558780149 1103 7 0.15680182564881306 1103 8 0.15723548505089013 1103 9 0.24776640491310076 1103 10-11 0.23592246278328588 1103 12-13 0.2790189513763508 1103 14-15 0.3625525369441718 1103 16-17 0.4099158985903273 1103 18-19 0.3199485272065772 1103 20-21 0.320265540263712 1103 22-23 0.358034325235316 1103 24-25 0.35515222286051085 1103 26-27 0.3484002906688417 1103 28-29 0.3478975130451474 1103 30-31 0.3589665469655827 1103 32-33 0.3452125639333019 1103 34-35 0.32936420462080207 1103 36-37 0.3399632260530865 1103 38-39 0.3176861724343212 1103 40-41 0.3598698965793403 1103 42-43 0.3805118482106593 1103 44-45 0.36403989600345454 1103 46-47 0.4318267473573272 1103 48-49 0.46486649949814307 1103 50-51 0.4509379232259221 1103 52-53 0.4294022455548472 1103 54-55 0.42527128195557395 1103 56-57 0.43178681771053107 1103 58-59 0.36926672518467285 1103 60-61 0.3873593035715004 1103 62-63 0.3866480231202445 1103 64-65 0.41881856709389353 1103 66-67 0.3682597684944682 1103 68-69 0.3333730861011901 1103 70-71 0.3088119467302164 1103 72-73 0.3040409675305469 1103 74-75 0.31951908595757317 1103 76-77 0.2943724771593139 1103 78-79 0.3157494311743747 1103 80-81 0.35383012992240026 1103 82-83 0.3126047949220734 1103 84-85 0.3404416555111851 1103 86-87 0.3715811832882636 1103 88-89 0.361615927685321 1103 90-91 0.3817122008251488 1103 92-93 0.36136229801123676 1103 94-95 0.3354957018723539 1103 96-97 0.3046227285379146 1103 98-99 0.30202981288774566 1103 100 0.2962444516379641 1104 1 0.19296748742458192 1104 2 0.2293783298801344 1104 3 0.24732523982780208 1104 4 0.17273025719799762 1104 5 0.13333540120592602 1104 6 0.14802494328047544 1104 7 0.15812372913272554 1104 8 0.14574132828439446 1104 9 0.2198942677712239 1104 10-11 0.20742832539056621 1104 12-13 0.22377492844187685 1104 14-15 0.3278029124385782 1104 16-17 0.4083864917440394 1104 18-19 0.3481212638139368 1104 20-21 0.35273232188146864 1104 22-23 0.3522246389579635 1104 24-25 0.300839364891722 1104 26-27 0.28607423405866683 1104 28-29 0.32265184843788575 1104 30-31 0.33591837865435537 1104 32-33 0.3720095583465124 1104 34-35 0.3338906330035911 1104 36-37 0.3251627810679878 1104 38-39 0.3378307212139333 1104 40-41 0.3523222804207933 1104 42-43 0.33615272769862514 1104 44-45 0.3347767215792956 1104 46-47 0.3887120352159812 1104 48-49 0.4500220046592176 1104 50-51 0.41793540565441845 1104 52-53 0.37704909624520866 1104 54-55 0.376645545624541 1104 56-57 0.35950121151010705 1104 58-59 0.3270625806236609 1104 60-61 0.3292139043849929 1104 62-63 0.3072513225766542 1104 64-65 0.3197554422258122 1104 66-67 0.32093268711337686 1104 68-69 0.3442056793222008 1104 70-71 0.314028387522427 1104 72-73 0.2947404323044367 1104 74-75 0.3371054802629132 1104 76-77 0.3059455565805891 1104 78-79 0.34271732270568833 1104 80-81 0.3186407206492916 1104 82-83 0.25425811237571594 1104 84-85 0.27380880798384055 1104 86-87 0.2342507675779686 1104 88-89 0.28638927997096175 1104 90-91 0.2602253551107765 1104 92-93 0.26396654168443234 1104 94-95 0.2612181653604253 1104 96-97 0.2820055758103379 1104 98-99 0.28741372469830395 1104 100 0.36508376857972635 1105 1 0.10090399219762958 1105 2 0.10481003085785545 1105 3 0.11083456000214653 1105 4 0.10069208990890388 1105 5 0.0822379663714159 1105 6 0.07976398694012943 1105 7 0.08233226666354199 1105 8 0.09038341314722231 1105 9 0.12547805880207363 1105 10-11 0.12401116349191454 1105 12-13 0.13393532922460594 1105 14-15 0.23068928947579792 1105 16-17 0.29597245683214624 1105 18-19 0.25070117735843667 1105 20-21 0.19300939460276823 1105 22-23 0.22555949644702622 1105 24-25 0.2029798119514652 1105 26-27 0.2311005228979468 1105 28-29 0.21394744819268396 1105 30-31 0.2672328604779395 1105 32-33 0.2656013281333216 1105 34-35 0.24511861661673606 1105 36-37 0.276812114971122 1105 38-39 0.22183172681506136 1105 40-41 0.20537319225218198 1105 42-43 0.1964546158770304 1105 44-45 0.22559685307616206 1105 46-47 0.2920359996418469 1105 48-49 0.2824063754766257 1105 50-51 0.2877240956027052 1105 52-53 0.26137609655014415 1105 54-55 0.2557895148506475 1105 56-57 0.20463472271222116 1105 58-59 0.26384918961536385 1105 60-61 0.19198845527390773 1105 62-63 0.1936857006969106 1105 64-65 0.15321404388789972 1105 66-67 0.1977390038372988 1105 68-69 0.18995072242326216 1105 70-71 0.18392089753074004 1105 72-73 0.25293818837896254 1105 74-75 0.26067392932815636 1105 76-77 0.1924177443922659 1105 78-79 0.2493678566679307 1105 80-81 0.1658487824271404 1105 82-83 0.19399179890319118 1105 84-85 0.1518041654775999 1105 86-87 0.17999390311175745 1105 88-89 0.23118351467633147 1105 90-91 0.19676343940646746 1105 92-93 0.21399659298659657 1105 94-95 0.25947658492102477 1105 96-97 0.23052917569155795 1105 98-99 0.2595013463804605 1105 100 0.25536656400281643 1106 1 0.02751522808909357 1106 2 -0.019718048780660524 1106 3 0.019645995744483002 1106 4 0.04298836119104266 1106 5 0.05152830716528456 1106 6 0.07779064885711051 1106 7 0.12921172737549824 1106 8 0.10938882223772595 1106 9 0.11960783727163005 1106 10-11 0.09142262430609094 1106 12-13 0.11380769206129315 1106 14-15 0.22702864725282978 1106 16-17 0.24395343338142084 1106 18-19 0.1671678323044432 1106 20-21 0.150718486457059 1106 22-23 0.14921231551004865 1106 24-25 0.1600663851474735 1106 26-27 0.15226642050108552 1106 28-29 0.11304028298756919 1106 30-31 0.14753974704124317 1106 32-33 0.19393118054617275 1106 34-35 0.16075903049293316 1106 36-37 0.14266225051370895 1106 38-39 0.12770188254014414 1106 40-41 0.14616512570015772 1106 42-43 0.1371861180739984 1106 44-45 0.1576577868607245 1106 46-47 0.14774717511303237 1106 48-49 0.20838237034117668 1106 50-51 0.14318981709974565 1106 52-53 0.1008952886270933 1106 54-55 0.23037548675021924 1106 56-57 0.18659997002684037 1106 58-59 0.1913223934006183 1106 60-61 0.12404598911513887 1106 62-63 0.10422260768510228 1106 64-65 0.1682612334563487 1106 66-67 0.17134631301356507 1106 68-69 0.10707312773182309 1106 70-71 0.12848290202327917 1106 72-73 0.17245459250237616 1106 74-75 0.19363447715334559 1106 76-77 0.16837112414980737 1106 78-79 0.17232478410183916 1106 80-81 0.13757644389456658 1106 82-83 0.10929024428458689 1106 84-85 0.11897516255704943 1106 86-87 0.09304029731281815 1106 88-89 0.15302890740762365 1106 90-91 0.0710080898972194 1106 92-93 0.054540401859775045 1106 94-95 0.08558481016266484 1106 96-97 0.08067349231885146 1106 98-99 0.034151648498323084 1106 100 0.16709789302202083 1107 1 0.07008439600427607 1107 2 0.10459815977591091 1107 3 0.09874193801264397 1107 4 0.05983979875932022 1107 5 0.08926699422804063 1107 6 0.08839208019963252 1107 7 0.0837835862863372 1107 8 0.10671009460347136 1107 9 0.13635061896990663 1107 10-11 0.1340906441599401 1107 12-13 0.1519151013116229 1107 14-15 0.2381333558084222 1107 16-17 0.33287667651561037 1107 18-19 0.2316573455319073 1107 20-21 0.19074983859063366 1107 22-23 0.20477422206747065 1107 24-25 0.21173974845847 1107 26-27 0.20739941816948004 1107 28-29 0.21560074218065495 1107 30-31 0.2500086417098544 1107 32-33 0.23325354329782044 1107 34-35 0.2642949153194962 1107 36-37 0.2306148782320605 1107 38-39 0.26537581647139774 1107 40-41 0.2781847339822434 1107 42-43 0.23912566598484375 1107 44-45 0.2727424799827318 1107 46-47 0.32154200326139204 1107 48-49 0.41539707272553983 1107 50-51 0.42565208860982295 1107 52-53 0.35857614455328957 1107 54-55 0.36508218484328836 1107 56-57 0.336678629536884 1107 58-59 0.31373146206856006 1107 60-61 0.3183178351445477 1107 62-63 0.34346765569055293 1107 64-65 0.34668413321702474 1107 66-67 0.32224780809212206 1107 68-69 0.3257922299475524 1107 70-71 0.26164468141269737 1107 72-73 0.293573985459723 1107 74-75 0.2594608901082509 1107 76-77 0.2507424314296074 1107 78-79 0.17092882486291927 1107 80-81 0.2800909902810744 1107 82-83 0.1928385738126437 1107 84-85 0.17706449115604528 1107 86-87 0.18726211970007256 1107 88-89 0.2636820007498102 1107 90-91 0.19893361315322267 1107 92-93 0.1854832029457114 1107 94-95 0.24145249452283934 1107 96-97 0.24302920348370805 1107 98-99 0.2550893358117392 1107 100 0.17420765684112283 1108 1 0.029611578527486415 1108 2 0.04473411092686064 1108 3 0.03830430841068022 1108 4 0.06007300294782425 1108 5 0.046022305860155654 1108 6 0.07728397560281053 1108 7 0.0669004527494792 1108 8 0.05779428553816501 1108 9 0.10750114693698976 1108 10-11 0.08845716720905727 1108 12-13 0.07169712197332245 1108 14-15 0.15786896619422208 1108 16-17 0.2012658384014685 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2214 78-79 -0.1460253727259726 2214 80-81 -0.12825185166133934 2214 82-83 -0.18292259988861304 2214 84-85 -0.15694572176734312 2214 86-87 -0.1048815048319014 2214 88-89 -0.02662847150832448 2214 90-91 -0.11864744433057695 2214 92-93 -0.11195135312978977 2214 94-95 -0.12962677565173664 2214 96-97 -0.1110224663815842 2214 98-99 -0.08856664944260473 2214 100 -0.26769950166174183 2215 1 -0.4135174301407716 2215 2 -0.40519388992869665 2215 3 -0.38906699937078315 2215 4 -0.31189174815212084 2215 5 -0.2662490853831798 2215 6 -0.337214361605227 2215 7 -0.3081082590284012 2215 8 -0.2676229335963285 2215 9 -0.40384151051673456 2215 10-11 -0.3955906212884628 2215 12-13 -0.3881130721319934 2215 14-15 -0.40924065102072404 2215 16-17 -0.41589144995080574 2215 18-19 -0.502397748693177 2215 20-21 -0.49570362977005544 2215 22-23 -0.5242379338985614 2215 24-25 -0.5007094210919973 2215 26-27 -0.489285276713467 2215 28-29 -0.5078072913889002 2215 30-31 -0.44322247102972057 2215 32-33 -0.48062085089399176 2215 34-35 -0.488890437503116 2215 36-37 -0.5308445171271927 2215 38-39 -0.5126713114383179 2215 40-41 -0.5640810287329501 2215 42-43 -0.5242172012493 2215 44-45 -0.5152969679567931 2215 46-47 -0.43645249979443435 2215 48-49 -0.3813113755574449 2215 50-51 -0.45765365281749837 2215 52-53 -0.44643947182640176 2215 54-55 -0.4221283398137743 2215 56-57 -0.39411643488262627 2215 58-59 -0.5041272022498333 2215 60-61 -0.5065464826646675 2215 62-63 -0.4577683342872447 2215 64-65 -0.3583206394434981 2215 66-67 -0.43178818021804943 2215 68-69 -0.4152747197813227 2215 70-71 -0.44509462193634874 2215 72-73 -0.42551099741126563 2215 74-75 -0.3855255997197915 2215 76-77 -0.33281975549386544 2215 78-79 -0.33397138454221675 2215 80-81 -0.3393953505438674 2215 82-83 -0.3607441041075745 2215 84-85 -0.30291372894301105 2215 86-87 -0.2901096244778856 2215 88-89 -0.2794446405778359 2215 90-91 -0.33247072835078484 2215 92-93 -0.2966240928691093 2215 94-95 -0.2582028551797819 2215 96-97 -0.3136466277836405 2215 98-99 -0.30270082484846483 2215 100 -0.49019919306238435 2216 1 -0.3800849375908655 2216 2 -0.4291974130256726 2216 3 -0.40538430429922556 2216 4 -0.30865499948399133 2216 5 -0.2669796246340894 2216 6 -0.2759194034782979 2216 7 -0.2932911802369915 2216 8 -0.3163568261671017 2216 9 -0.36470349579823846 2216 10-11 -0.41438658172164367 2216 12-13 -0.38353092860148763 2216 14-15 -0.5268163099277317 2216 16-17 -0.4737340621295374 2216 18-19 -0.49023060819747855 2216 20-21 -0.5012472439761027 2216 22-23 -0.5103094430294348 2216 24-25 -0.47716038302071695 2216 26-27 -0.48213233016763724 2216 28-29 -0.5258900674944726 2216 30-31 -0.5127573882478416 2216 32-33 -0.5728338505461963 2216 34-35 -0.5821640210846155 2216 36-37 -0.5714145032090272 2216 38-39 -0.5184448585665535 2216 40-41 -0.566690044278019 2216 42-43 -0.4987530592786058 2216 44-45 -0.4639388128672479 2216 46-47 -0.5094025964811664 2216 48-49 -0.4639481821196938 2216 50-51 -0.4724159011457587 2216 52-53 -0.494018128776581 2216 54-55 -0.4079036503290183 2216 56-57 -0.45104333315621403 2216 58-59 -0.5077205906294964 2216 60-61 -0.5218648255284748 2216 62-63 -0.47840717361025753 2216 64-65 -0.42222836146194 2216 66-67 -0.46156769122698904 2216 68-69 -0.44795541131811945 2216 70-71 -0.40528252297771417 2216 72-73 -0.3934412387978057 2216 74-75 -0.41179113499126885 2216 76-77 -0.4112251702426093 2216 78-79 -0.36434326108395254 2216 80-81 -0.43466123969729153 2216 82-83 -0.3979978100097483 2216 84-85 -0.3659598860019315 2216 86-87 -0.2657502462069985 2216 88-89 -0.25355242634827846 2216 90-91 -0.29982865349948895 2216 92-93 -0.3262123589629695 2216 94-95 -0.2715146519250098 2216 96-97 -0.3355147187015035 2216 98-99 -0.3047037731884217 2216 100 -0.4062637627598171 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 40.0 8 145.0 9 327.0 10 436.0 11 279.0 12 256.0 13 345.0 14 617.0 15 1174.0 16 2226.0 17 3961.0 18 6646.0 19 9953.0 20 14489.0 21 21426.0 22 30039.0 23 33378.0 24 31319.0 25 26331.0 26 24717.0 27 27942.0 28 34129.0 29 41350.0 30 51004.0 31 64097.0 32 81703.0 33 106969.0 34 153710.0 35 237799.0 36 423065.0 37 956813.0 38 1333554.0 39 471252.0 40 966.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.301896954715865 20.06597252949479 13.621330847536385 24.010799668252968 2 15.114142268037304 21.204099313219054 39.72612248325029 23.95563593549335 3 17.452381043247286 26.687248118521683 31.399602332575142 24.460768505655885 4 11.984809755624926 18.114220843701474 39.426358982999155 30.474610417674448 5 12.21032218360673 38.61382572459706 35.776525225829076 13.399326865967135 6 28.953252083427255 39.498946985760156 18.07942797318195 13.46837295763064 7 25.244105125969124 34.09156828360273 23.328139877914044 17.336186712514095 8 25.48003072196621 35.042457375657136 20.90062111801242 18.576890784364235 9 26.3600745529945 14.709427938863492 21.014971183941434 37.91552632420058 10-11 24.048605911622623 26.317635522688033 29.176952013534795 20.45680655215455 12-13 25.30569782826634 24.499893499205836 28.819424981580017 21.374983690947815 14-15 22.406722932810744 25.439539496657805 27.152986220964515 25.000751349566936 16-17 21.181057408842307 28.199691993684358 27.99023195644834 22.629018641024995 18-19 20.988629324469148 27.11480706631992 30.655516307763648 21.241047301447292 20-21 22.175650330845283 26.144291049395306 30.36827215062912 21.31178646913029 22-23 22.056517216515278 26.171311953825644 30.092008099307876 21.6801627303512 24-25 21.66858240883568 26.377145907519147 30.393239572880532 21.561032110764643 26-27 21.688764962190895 26.423773253896744 30.152530416338447 21.734931367573914 28-29 21.409021010829687 26.671114337010494 30.286869966704487 21.63299468545533 30-31 21.833415922322224 26.367450039624824 30.117765338743062 21.68136869930989 32-33 21.73231849195398 26.598488471524373 29.75131456703666 21.917878469484993 34-35 22.005434294541722 26.51144074876531 30.01020763880582 21.47291731788715 36-37 22.08800884732532 26.272646792623938 29.924756470167168 21.714587889883575 38-39 21.952989254785628 26.387356528424068 29.95189299175215 21.707761225038162 40-41 21.980817311981177 26.23476240248486 29.968113927353755 21.816306358180203 42-43 22.020595560073723 26.293936944374146 29.6041915277843 22.081275967767827 44-45 22.034407898887483 26.61562000253552 29.39749742303912 21.952474675537875 46-47 22.22902942117236 26.668097013278846 28.845292867643007 22.257580697905787 48-49 22.493222346625625 26.670835267363696 28.610689586576264 22.22525279943441 50-51 22.1990827812903 26.93443248195509 28.273515983586712 22.59296875316789 52-53 22.15915630008645 27.159527802256374 28.121545491961303 22.559770405695872 54-55 22.166176856086327 27.045653876253834 28.078501835567238 22.709667432092598 56-57 22.357998025259928 26.925732337209467 27.98574485256296 22.730524784967646 58-59 22.067464121566037 26.78062389249553 28.41758974110663 22.734322244831805 60-61 22.122899071469735 26.993306033347707 28.229640933949558 22.654153961233003 62-63 22.04058486088087 26.84592541354101 28.284678409998925 22.828811315579195 64-65 21.749966189148516 26.806197153400607 28.25667671888525 23.187159938565625 66-67 21.946960070079065 26.844861812693544 28.096362293026147 23.111815824201248 68-69 21.856568768288486 26.678032586677062 28.2549257786341 23.210472866400348 70-71 21.984211605984278 26.444510438134493 28.19145485077475 23.37982310510648 72-73 22.131539649510817 26.432072967355584 28.275804410448053 23.160582972685546 74-75 21.989518055735775 26.81207350909466 28.02616526474876 23.172243170420803 76-77 21.96096007953632 26.587813343921002 27.932147484249985 23.51907909229269 78-79 21.843135823742177 26.71494684195512 27.88057878009848 23.561338554204223 80-81 21.93327219919853 26.7194213939179 27.939993137615847 23.407313269267718 82-83 22.061763702673627 26.35207982005663 28.22400135482286 23.362155122446886 84-85 22.08140764119728 26.512797345311746 27.73607300011068 23.6697220133803 86-87 21.640854038574517 26.751806876015667 27.861704962030622 23.745634123379205 88-89 21.949479744216816 26.3302521647807 27.680283900347696 24.03998419065479 90-91 21.81060457164011 26.205307819569246 27.898849743443936 24.08523786534671 92-93 21.984460160526663 26.221445694045247 27.72807069851757 24.066023446910517 94-95 21.434945982069436 26.12511465549802 27.981940395002873 24.45799896742967 96-97 21.497161736025053 26.046279260559395 27.969515123593315 24.487043879822238 98-99 21.30751708428246 26.2460017412253 27.753056088921753 24.69342508557049 100 21.53083139165861 25.85661453247154 27.617453517369327 24.995100558500525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 396.0 1 270.0 2 392.5 3 649.0 4 449.0 5 325.5 6 699.5 7 1840.0 8 3443.0 9 4141.5 10 3125.0 11 2032.5 12 2037.5 13 2370.5 14 2809.5 15 3296.5 16 3783.5 17 4415.5 18 5094.5 19 5631.5 20 6448.5 21 7560.0 22 9300.0 23 11650.5 24 14389.0 25 18321.0 26 23422.5 27 29720.0 28 36689.0 29 44052.5 30 52821.5 31 62098.0 32 73150.5 33 83002.5 34 90064.0 35 97387.5 36 105474.0 37 117061.5 38 122783.5 39 122134.5 40 123774.0 41 126459.0 42 129885.0 43 134969.0 44 140971.0 45 151950.0 46 163562.0 47 169342.5 48 171694.5 49 167309.5 50 160382.5 51 158701.0 52 155238.0 53 148430.5 54 141563.0 55 127879.0 56 115294.5 57 102891.5 58 89208.0 59 79146.5 60 65168.0 61 50136.0 62 38920.5 63 29548.5 64 20685.0 65 14253.5 66 10452.0 67 7458.5 68 5322.0 69 3934.0 70 3063.0 71 2361.0 72 1832.0 73 1641.5 74 1302.0 75 952.0 76 720.0 77 500.0 78 339.0 79 216.5 80 154.5 81 114.0 82 74.0 83 40.0 84 27.5 85 20.5 86 14.0 87 9.5 88 6.5 89 3.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0025283503205876653 2 0.003458592419671806 3 2.1467125363480174E-4 4 0.050948644195992945 5 0.0030292499124022023 6 0.004913586472085462 7 2.385236151497797E-5 8 4.0549014575462554E-4 9 7.155708454493391E-5 10-11 9.540944605991187E-5 12-13 0.0 14-15 2.385236151497797E-5 16-17 2.504497959072687E-4 18-19 1.4311416908986783E-4 20-21 3.339330612096916E-4 22-23 0.0 24-25 0.0 26-27 3.4585924196718057E-4 28-29 0.0 30-31 2.2659743439229073E-4 32-33 2.8622833817973565E-4 34-35 2.0274507287731277E-4 36-37 8.34832653024229E-5 38-39 7.75201749236784E-4 40-41 7.394232069643171E-4 42-43 0.0 44-45 4.412686880270924E-4 46-47 0.0 48-49 7.155708454493391E-5 50-51 0.0 52-53 4.412686880270924E-4 54-55 4.770472302995594E-4 56-57 1.5504034984735682E-4 58-59 8.58685014539207E-4 60-61 5.963090378744493E-4 62-63 1.669665306048458E-4 64-65 3.816377842396475E-4 66-67 0.0011806918949914095 68-69 0.002373309970740308 70-71 0.0032677735275519816 72-73 0.0027788001164949336 74-75 0.002504497959072687 76-77 0.003780599300124008 78-79 0.0012641751602938327 80-81 8.70611195296696E-4 82-83 6.440137609044053E-4 84-85 5.963090378744493E-4 86-87 0.0 88-89 0.0 90-91 2.385236151497797E-5 92-93 1.669665306048458E-4 94-95 1.5504034984735682E-4 96-97 4.174163265121145E-4 98-99 1.669665306048458E-4 100 0.07642296629398942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4192457.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.421412235015026 #Duplication Level Percentage of deduplicated Percentage of total 1 67.64148102730914 34.105789990649015 2 15.728163907883836 15.860724721985914 3 6.616621642760208 10.008582223582046 4 3.378804599735637 6.81456398339342 5 1.9560837071894592 4.931425148319808 6 1.2315321520388702 3.725735419117624 7 0.8108447431177004 2.86187559359327 8 0.577796963277072 2.3306671098826466 9 0.414007454175666 1.8787356463824314 >10 1.5942718409923629 12.621028773935661 >50 0.03335719925538172 1.125412391306145 >100 0.014970911071778465 1.337322671055284 >500 0.0010732026203387334 0.3803684889575138 >1k 7.429864294630182E-4 0.5179388387051619 >5k 8.255404771811312E-5 0.3223433550536667 >10k+ 1.6510809543622624E-4 1.1774856440803358 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14988 0.3574991943864898 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13300 0.317236408149207 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7744 0.18471268757198941 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.3852361514977972E-5 0.0 3 0.0 0.0 0.0 4.7704723029955944E-5 0.0 4 0.0 0.0 0.0 7.155708454493391E-5 0.0 5 0.0 0.0 0.0 7.155708454493391E-5 0.0 6 0.0 0.0 0.0 7.155708454493391E-5 0.0 7 0.0 0.0 0.0 7.155708454493391E-5 0.0 8 0.0 0.0 0.0 7.155708454493391E-5 0.0 9 0.0 0.0 0.0 1.1926180757488985E-4 0.0 10-11 0.0 0.0 0.0 1.9081889211982378E-4 0.0 12-13 0.0 0.0 0.0 2.7430215742224666E-4 0.0 14-15 0.0 0.0 0.0 2.8622833817973565E-4 2.3852361514977972E-5 16-17 2.3852361514977972E-5 0.0 0.0 5.128257725720264E-4 4.7704723029955944E-5 18-19 2.3852361514977972E-5 0.0 0.0 8.70611195296696E-4 4.7704723029955944E-5 20-21 2.3852361514977972E-5 0.0 0.0 0.0010017991836290749 4.7704723029955944E-5 22-23 2.3852361514977972E-5 0.0 0.0 0.0013357322448387665 4.7704723029955944E-5 24-25 2.3852361514977972E-5 0.0 0.0 0.0020155245480156384 4.7704723029955944E-5 26-27 2.3852361514977972E-5 0.0 0.0 0.003076954635432158 7.155708454493391E-5 28-29 2.3852361514977972E-5 0.0 0.0 0.007895131661457708 7.155708454493391E-5 30-31 2.3852361514977972E-5 0.0 0.0 0.019797460057431715 7.155708454493391E-5 32-33 2.3852361514977972E-5 0.0 0.0 0.0468818165576892 7.155708454493391E-5 34-35 2.3852361514977972E-5 0.0 0.0 0.08719230751800197 7.155708454493391E-5 36-37 2.3852361514977972E-5 0.0 0.0 0.15241659008070924 7.155708454493391E-5 38-39 2.3852361514977972E-5 0.0 0.0 0.25198827322498474 7.155708454493391E-5 40-41 2.3852361514977972E-5 0.0 0.0 0.3644521577681059 7.155708454493391E-5 42-43 2.3852361514977972E-5 0.0 0.0 0.4744115443521544 7.155708454493391E-5 44-45 2.3852361514977972E-5 0.0 0.0 0.6135423690690209 7.155708454493391E-5 46-47 2.3852361514977972E-5 0.0 0.0 0.7830014714521818 7.155708454493391E-5 48-49 2.3852361514977972E-5 0.0 0.0 0.9716378724933852 7.155708454493391E-5 50-51 2.3852361514977972E-5 0.0 0.0 1.1772929334755253 7.155708454493391E-5 52-53 2.3852361514977972E-5 0.0 0.0 1.3739198756242461 7.155708454493391E-5 54-55 2.3852361514977972E-5 0.0 0.0 1.5879709678596585 7.155708454493391E-5 56-57 2.3852361514977972E-5 0.0 0.0 1.8473057684312564 7.155708454493391E-5 58-59 2.3852361514977972E-5 0.0 0.0 2.1456391800798436 7.155708454493391E-5 60-61 2.3852361514977972E-5 0.0 0.0 2.4627444002407177 7.155708454493391E-5 62-63 2.3852361514977972E-5 0.0 0.0 2.778370773987664 7.155708454493391E-5 64-65 2.3852361514977972E-5 0.0 0.0 3.0992088887256326 7.155708454493391E-5 66-67 2.3852361514977972E-5 0.0 0.0 3.4693379085343032 7.155708454493391E-5 68-69 2.3852361514977972E-5 0.0 0.0 3.888221155279589 7.155708454493391E-5 70-71 2.3852361514977972E-5 0.0 0.0 4.340843567387811 7.155708454493391E-5 72-73 2.3852361514977972E-5 0.0 0.0 4.809888330399096 7.155708454493391E-5 74-75 2.3852361514977972E-5 0.0 0.0 5.2653968782506295 8.348326530242291E-5 76-77 2.3852361514977972E-5 0.0 0.0 5.762074602077016 9.540944605991189E-5 78-79 2.3852361514977972E-5 0.0 0.0 6.316045698262379 9.540944605991189E-5 80-81 2.3852361514977972E-5 0.0 0.0 6.918007745815878 9.540944605991189E-5 82-83 2.3852361514977972E-5 0.0 0.0 7.540983723864073 9.540944605991189E-5 84-85 2.3852361514977972E-5 0.0 0.0 8.151043648151909 9.540944605991189E-5 86-87 2.3852361514977972E-5 0.0 0.0 8.77450859961116 1.1926180757488986E-4 88 2.3852361514977972E-5 0.0 0.0 9.276231097897963 1.4311416908986783E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10295 0.0 50.977863 1 GTATCAA 17935 0.0 39.461987 1 ATCAACG 21720 0.0 32.06328 3 CAACGCA 21870 0.0 31.848526 5 TATCAAC 22505 0.0 31.176203 2 AACGCAG 23280 0.0 30.26204 6 TCAACGC 23335 0.0 29.903942 4 ACGCAGA 25625 0.0 27.198597 7 CGCAGAG 25795 0.0 26.982906 8 GTACATG 13285 0.0 26.088537 1 TACATGG 13535 0.0 24.633234 2 GCAGAGT 28180 0.0 24.315641 9 ACATGGG 13480 0.0 23.965117 3 GAGTACT 19010 0.0 23.07817 12-13 AGAGTAC 24560 0.0 21.87193 10-11 CATGGGG 9675 0.0 21.676353 4 CAGAGTA 26975 0.0 21.664743 10-11 GTACTTT 20505 0.0 21.017513 14-15 AGTACTT 19840 0.0 20.442705 12-13 GTACTAG 1825 0.0 18.81065 1 >>END_MODULE