FastQCFastQC Report
Mon 16 Jan 2017
ERR1146519_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146519_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5167725
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87000.028.2157971
GTATCAA121750.025.1450821
GTACATG160450.025.1178321
GTACTAG22550.024.6080111
TACATGG157450.024.1552892
ACATGGG154700.023.4906793
ATCAACG129550.022.172273
CAACGCA130100.022.004355
CGACGAG10300.021.67104124-25
CTGTGCG26050.021.6455979
AACGCAG137650.021.5759816
GTGATCG14000.021.1464778
TATCAAC143800.020.9558622
GCATCGT22300.020.2298138
CATGGGG102300.020.172984
CTAGTAC32150.019.8867723
TCAACGC147650.019.6441334
ACGCAGA146250.019.6009127
CGCAGAG146450.019.5733858
CAATGCG11300.019.54513418-19
Produced by FastQC (version 0.11.5)