##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146519_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5167725
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21026389755647	31.0	31.0	34.0	28.0	34.0
2	31.24068327939277	31.0	31.0	34.0	28.0	34.0
3	31.438766188216285	33.0	31.0	34.0	28.0	34.0
4	34.8879994581755	37.0	35.0	37.0	32.0	37.0
5	34.93128678480376	37.0	35.0	37.0	32.0	37.0
6	34.93835159572152	37.0	35.0	37.0	32.0	37.0
7	34.904039785398794	37.0	35.0	37.0	32.0	37.0
8	34.84108577759072	37.0	35.0	37.0	32.0	37.0
9	36.410402062803264	39.0	37.0	39.0	32.0	39.0
10-11	36.4126476350812	39.0	37.0	39.0	32.0	39.0
12-13	36.35599533643915	39.0	37.0	39.0	32.0	39.0
14-15	37.69008906240174	40.0	38.0	41.0	32.0	41.0
16-17	37.72796791624941	40.0	38.0	41.0	32.0	41.0
18-19	37.76594526605034	40.0	38.0	41.0	32.0	41.0
20-21	37.88623678698073	40.0	38.0	41.0	32.0	41.0
22-23	37.91385899985003	40.0	38.0	41.0	32.0	41.0
24-25	37.9544090484691	40.0	38.0	41.0	32.0	41.0
26-27	38.02552467478436	40.0	38.0	41.0	32.0	41.0
28-29	38.011333323657894	40.0	38.0	41.0	32.0	41.0
30-31	37.978240328190836	40.0	38.0	41.0	32.0	41.0
32-33	37.94550474338321	40.0	38.0	41.0	31.0	41.0
34-35	37.89562573472853	40.0	38.0	41.0	31.0	41.0
36-37	37.82339665907145	40.0	38.0	41.0	31.0	41.0
38-39	37.76636779240381	40.0	38.0	41.0	31.0	41.0
40-41	37.71770121281608	40.0	38.0	41.0	31.0	41.0
42-43	37.56621182435211	40.0	37.0	41.0	30.0	41.0
44-45	37.4575770769536	40.0	37.0	41.0	30.0	41.0
46-47	37.35807303987731	40.0	37.0	41.0	30.0	41.0
48-49	37.26032722329458	40.0	36.5	41.0	30.0	41.0
50-51	37.13715716296823	40.0	36.0	41.0	30.0	41.0
52-53	37.025030163176254	40.0	36.0	41.0	30.0	41.0
54-55	36.92565703476868	39.5	35.5	41.0	30.0	41.0
56-57	36.758789796283665	39.0	35.0	41.0	30.0	41.0
58-59	36.58747185657131	39.0	35.0	41.0	29.5	41.0
60-61	36.387826171090765	39.0	35.0	41.0	29.0	41.0
62-63	36.171061347111156	38.5	35.0	40.5	29.0	41.0
64-65	35.898706200504094	38.0	35.0	40.0	29.0	41.0
66-67	35.677583269233565	37.0	35.0	40.0	28.5	41.0
68-69	35.43512290224422	37.0	34.0	40.0	28.0	41.0
70-71	35.1616840098883	36.5	34.0	39.5	28.0	41.0
72-73	34.82235490472113	36.0	34.0	39.0	28.0	41.0
74-75	34.4827326337992	35.5	34.0	39.0	28.0	40.5
76-77	34.19576883444843	35.0	34.0	37.5	28.0	40.0
78-79	33.8614577981607	35.0	34.0	37.0	27.0	39.0
80-81	33.59372857495319	35.0	33.5	37.0	27.0	39.0
82-83	33.29255146123295	35.0	33.0	36.0	26.5	38.5
84-85	33.057082468591105	35.0	33.0	36.0	26.0	37.0
86-87	32.81712252490216	35.0	33.0	35.5	26.0	37.0
88-89	32.60699950171497	35.0	33.0	35.0	26.0	36.5
90-91	32.43326657281493	35.0	33.0	35.0	25.5	36.0
92-93	32.25720360506799	35.0	33.0	35.0	25.0	36.0
94-95	32.11855110324176	35.0	33.0	35.0	25.0	36.0
96-97	32.008578629861304	35.0	33.0	35.0	25.0	35.5
98-99	31.88583293422154	35.0	32.5	35.0	25.0	35.0
100	30.747664397776585	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39539681484294675
1101	2	0.4313093002982349
1101	3	0.4352443909958694
1101	4	0.4860570325190352
1101	5	0.4033867344842861
1101	6	0.4477062878710214
1101	7	0.4262878919942068
1101	8	0.4824602309549917
1101	9	0.5798815597781299
1101	10-11	0.6479682534143976
1101	12-13	0.6244093722552222
1101	14-15	0.7365611510980656
1101	16-17	0.6861247478983614
1101	18-19	0.687886898268026
1101	20-21	0.7352970558797409
1101	22-23	0.6931926154362742
1101	24-25	0.7222075079989381
1101	26-27	0.6941903198921295
1101	28-29	0.6543794408144734
1101	30-31	0.7267088094905958
1101	32-33	0.7446319829012182
1101	34-35	0.6724680855031622
1101	36-37	0.7320448748441919
1101	38-39	0.7340128247225763
1101	40-41	0.7092837423146605
1101	42-43	0.6941814438466736
1101	44-45	0.6464715153057767
1101	46-47	0.6784620095106177
1101	48-49	0.6626434469725169
1101	50-51	0.6610195938805674
1101	52-53	0.5915117130283463
1101	54-55	0.6058661526010098
1101	56-57	0.5798307606358506
1101	58-59	0.5600069133242158
1101	60-61	0.6439108355581098
1101	62-63	0.5549285930650001
1101	64-65	0.6789393932215617
1101	66-67	0.569341850271293
1101	68-69	0.5409779080070152
1101	70-71	0.5694526092664347
1101	72-73	0.537608761458678
1101	74-75	0.48440563573024775
1101	76-77	0.44897108438226496
1101	78-79	0.41762855687665024
1101	80-81	0.4145552043141407
1101	82-83	0.36753288299227194
1101	84-85	0.35380978251313877
1101	86-87	0.4638888164691579
1101	88-89	0.485614538609795
1101	90-91	0.411186706138011
1101	92-93	0.4761985315684285
1101	94-95	0.4653725672642324
1101	96-97	0.4155947771670512
1101	98-99	0.45499020215066466
1101	100	0.5746004289586182
1102	1	0.3240418209105904
1102	2	0.34832526610839665
1102	3	0.31779596594442694
1102	4	0.3549058480099916
1102	5	0.32934895682375753
1102	6	0.3680443662754982
1102	7	0.38171495894930985
1102	8	0.4096234030121835
1102	9	0.544255242044791
1102	10-11	0.5499951093211166
1102	12-13	0.525761907473921
1102	14-15	0.5937719567752708
1102	16-17	0.5020047879975564
1102	18-19	0.5015034972233394
1102	20-21	0.5604057246920675
1102	22-23	0.545410079689951
1102	24-25	0.572371355410958
1102	26-27	0.5032524947457162
1102	28-29	0.558598994447145
1102	30-31	0.5516677605801377
1102	32-33	0.5468586172071426
1102	34-35	0.5093094509862013
1102	36-37	0.5782242132075055
1102	38-39	0.4767121863012207
1102	40-41	0.48554151680946944
1102	42-43	0.5489844512861239
1102	44-45	0.49397928716247463
1102	46-47	0.5015398336017896
1102	48-49	0.5003772621996561
1102	50-51	0.4724340190375358
1102	52-53	0.4656093967723436
1102	54-55	0.4406440980450057
1102	56-57	0.499452435304768
1102	58-59	0.503990704808416
1102	60-61	0.5013353804850382
1102	62-63	0.4685360325204968
1102	64-65	0.5257327346450822
1102	66-67	0.516853621494846
1102	68-69	0.5033616889387886
1102	70-71	0.4367513588047629
1102	72-73	0.48801628533352215
1102	74-75	0.5903099358410415
1102	76-77	0.5368905905290546
1102	78-79	0.46661928130022545
1102	80-81	0.43382556784498405
1102	82-83	0.45396573267306195
1102	84-85	0.43924574515757797
1102	86-87	0.41319372862027137
1102	88-89	0.44893850842987604
1102	90-91	0.46524606349568387
1102	92-93	0.42033086945689035
1102	94-95	0.41879909604595156
1102	96-97	0.44087401528239667
1102	98-99	0.512385865106399
1102	100	0.5333905307894611
1103	1	0.2228769441381857
1103	2	0.28111716965292644
1103	3	0.2899689895445441
1103	4	0.2733042849952483
1103	5	0.2382864787497354
1103	6	0.2762181412325688
1103	7	0.3248089310294304
1103	8	0.2648232390164722
1103	9	0.3571141536015574
1103	10-11	0.31739096835474356
1103	12-13	0.3397672786070487
1103	14-15	0.42340796220499755
1103	16-17	0.3903379863805654
1103	18-19	0.3830304653107248
1103	20-21	0.36360395811644963
1103	22-23	0.42217257986710166
1103	24-25	0.43888054942274124
1103	26-27	0.42445493895149866
1103	28-29	0.44843231685461404
1103	30-31	0.4421877860331236
1103	32-33	0.4696748917844289
1103	34-35	0.48726484014078864
1103	36-37	0.48232504048748126
1103	38-39	0.5123729560948433
1103	40-41	0.4362619153054581
1103	42-43	0.5113832975976607
1103	44-45	0.46745593497007576
1103	46-47	0.4910927521793482
1103	48-49	0.4292406715386079
1103	50-51	0.4705787905884691
1103	52-53	0.4124497837253145
1103	54-55	0.36909425484291347
1103	56-57	0.42401241784769894
1103	58-59	0.42305590271837445
1103	60-61	0.42964430562938105
1103	62-63	0.45293496436042346
1103	64-65	0.368528416107857
1103	66-67	0.4684913264698025
1103	68-69	0.4418218683876276
1103	70-71	0.3766834739607816
1103	72-73	0.4105011166830579
1103	74-75	0.4013969407553617
1103	76-77	0.395918008427806
1103	78-79	0.39009364586448925
1103	80-81	0.33234969758326116
1103	82-83	0.353983946822062
1103	84-85	0.34170199494158027
1103	86-87	0.2913788012808638
1103	88-89	0.25369953075601615
1103	90-91	0.3085363095547251
1103	92-93	0.30388909535278685
1103	94-95	0.29816692812256207
1103	96-97	0.3153456164177655
1103	98-99	0.28407669754874476
1103	100	0.3165229400902092
1104	1	0.0972683102851768
1104	2	0.12964652621595008
1104	3	0.1341480618507589
1104	4	0.18147826927685884
1104	5	0.1032231473938694
1104	6	0.13512752462884237
1104	7	0.16611484444555202
1104	8	0.20576912477120146
1104	9	0.21390057924105577
1104	10-11	0.24845359463144945
1104	12-13	0.2340653959063701
1104	14-15	0.30834834006086
1104	16-17	0.33147660796241496
1104	18-19	0.27533730477209417
1104	20-21	0.30098904898149215
1104	22-23	0.30562124317710015
1104	24-25	0.34496587212803576
1104	26-27	0.313431807943374
1104	28-29	0.2688102365769822
1104	30-31	0.33826732143935345
1104	32-33	0.30649800901603186
1104	34-35	0.3102602017879619
1104	36-37	0.3128741431368667
1104	38-39	0.3260534751290791
1104	40-41	0.3499232594371051
1104	42-43	0.3310511654529762
1104	44-45	0.27173374738264044
1104	46-47	0.26219741231919613
1104	48-49	0.2536528088843184
1104	50-51	0.27243319328929516
1104	52-53	0.2748771232279026
1104	54-55	0.21677997373922153
1104	56-57	0.27121011515666993
1104	58-59	0.28947707838950976
1104	60-61	0.2635492193965021
1104	62-63	0.21451849205489992
1104	64-65	0.27820093547335034
1104	66-67	0.28965764233830527
1104	68-69	0.2401709112097734
1104	70-71	0.20312952476285773
1104	72-73	0.2688766599558434
1104	74-75	0.22658784809782873
1104	76-77	0.23774299864686554
1104	78-79	0.21944393012129382
1104	80-81	0.18725749551887105
1104	82-83	0.14298383150175908
1104	84-85	0.178335133584838
1104	86-87	0.1501176905760815
1104	88-89	0.11617443020871576
1104	90-91	0.13038749450952736
1104	92-93	0.09952338990792953
1104	94-95	0.11273743545836368
1104	96-97	0.15306390749471888
1104	98-99	0.11671353302034149
1104	100	0.2238160978425796
1105	1	0.19836779923597803
1105	2	0.19417748515279243
1105	3	0.227689888050584
1105	4	0.26423049459470604
1105	5	0.226242628089679
1105	6	0.21908215475630755
1105	7	0.29491173361684986
1105	8	0.3010325513533658
1105	9	0.37223241907247484
1105	10-11	0.3592429239676278
1105	12-13	0.3637446731600633
1105	14-15	0.4523581754498025
1105	16-17	0.40330101558524944
1105	18-19	0.39557534182179666
1105	20-21	0.3674543644362487
1105	22-23	0.37952202577209704
1105	24-25	0.44983400948676433
1105	26-27	0.39519995127611196
1105	28-29	0.338567501784901
1105	30-31	0.4000863524553111
1105	32-33	0.43328016411212644
1105	34-35	0.38624482686467587
1105	36-37	0.4282960438488601
1105	38-39	0.42215176927835074
1105	40-41	0.4693123085380151
1105	42-43	0.4860979799491787
1105	44-45	0.399863584292234
1105	46-47	0.3701311660054287
1105	48-49	0.38511967215013954
1105	50-51	0.3824397918522706
1105	52-53	0.3893666714299968
1105	54-55	0.31803288606794666
1105	56-57	0.39992879913709345
1105	58-59	0.36816548501305135
1105	60-61	0.4254675004029522
1105	62-63	0.3543926848850063
1105	64-65	0.357738737364393
1105	66-67	0.35920147254614676
1105	68-69	0.3568755942886668
1105	70-71	0.35305525698611007
1105	72-73	0.3687123747458543
1105	74-75	0.3784123643389776
1105	76-77	0.38293826723281654
1105	78-79	0.3425873714755454
1105	80-81	0.33409955361880606
1105	82-83	0.3415363566061629
1105	84-85	0.334182600877142
1105	86-87	0.286944867966092
1105	88-89	0.27071861342609793
1105	90-91	0.24793098256965607
1105	92-93	0.24534973093686574
1105	94-95	0.26714896855165904
1105	96-97	0.2958883096866387
1105	98-99	0.2882312296602372
1105	100	0.36445184087335747
1106	1	0.11902468478322703
1106	2	0.10257565729889961
1106	3	0.0826232894696588
1106	4	0.09950786198155015
1106	5	0.1757344451677625
1106	6	0.09018432370380225
1106	7	0.13808935184822246
1106	8	0.20513999206978895
1106	9	0.16503988758171317
1106	10-11	0.19565239409124047
1106	12-13	0.23838574916298683
1106	14-15	0.20635650610802259
1106	16-17	0.21288891511800045
1106	18-19	0.18767296329788508
1106	20-21	0.23951895368995935
1106	22-23	0.20079341172028364
1106	24-25	0.2594985498038511
1106	26-27	0.24316019381382858
1106	28-29	0.22856709841234846
1106	30-31	0.21308439011435354
1106	32-33	0.22055872477248784
1106	34-35	0.21590113128949184
1106	36-37	0.19576617544140618
1106	38-39	0.164645870508906
1106	40-41	0.12350962154491896
1106	42-43	0.15087792692563085
1106	44-45	0.16018830362694558
1106	46-47	0.18392717208526932
1106	48-49	0.10400774690615577
1106	50-51	0.13805635565893937
1106	52-53	0.1361630733468573
1106	54-55	0.09883337224268729
1106	56-57	0.13283832533392825
1106	58-59	0.12690220197717395
1106	60-61	0.13994640291046778
1106	62-63	0.11299043778961959
1106	64-65	0.180893474177104
1106	66-67	0.1745352145712218
1106	68-69	0.13059383368717192
1106	70-71	0.09304968955839854
1106	72-73	0.15318089151839587
1106	74-75	0.13116396353382953
1106	76-77	0.1477432144419879
1106	78-79	0.1563895389602621
1106	80-81	0.14883045315288967
1106	82-83	0.08414814510242508
1106	84-85	0.056665256965700905
1106	86-87	0.09251788083545165
1106	88-89	0.13409814843063117
1106	90-91	0.1486969914731091
1106	92-93	0.16767086069916104
1106	94-95	0.09595783601008634
1106	96-97	0.1564213199673148
1106	98-99	0.1213339314141102
1106	100	0.19295955304620804
1107	1	0.025042686942125414
1107	2	0.03421985911450065
1107	3	0.03372237826930302
1107	4	0.02386007419207914
1107	5	0.017969074902310922
1107	6	0.08709659827222538
1107	7	0.11430823568208837
1107	8	0.10207717305029007
1107	9	0.1813881782986968
1107	10-11	0.17611513822838276
1107	12-13	0.12654349286356137
1107	14-15	0.14630846875647308
1107	16-17	0.15421765889472994
1107	18-19	0.11960732781906813
1107	20-21	0.17568733099471245
1107	22-23	0.20814227139553054
1107	24-25	0.20915253440480086
1107	26-27	0.21699791819368386
1107	28-29	0.21521611967449417
1107	30-31	0.23671311441066933
1107	32-33	0.21679452539172672
1107	34-35	0.19977646598282206
1107	36-37	0.17421281377548326
1107	38-39	0.1928054240239092
1107	40-41	0.22132143741810495
1107	42-43	0.2499316549759456
1107	44-45	0.23304099271670964
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2216	60-61	-0.5765143799390628
2216	62-63	-0.6318151598439812
2216	64-65	-0.5693875816262377
2216	66-67	-0.5187277492670006
2216	68-69	-0.6214408583996303
2216	70-71	-0.5916023085374533
2216	72-73	-0.540716880378497
2216	74-75	-0.5254031033087259
2216	76-77	-0.5408578881197315
2216	78-79	-0.5181509527392691
2216	80-81	-0.5425522892162888
2216	82-83	-0.49592302526085774
2216	84-85	-0.5191945404626779
2216	86-87	-0.5719200938505011
2216	88-89	-0.5020198664690554
2216	90-91	-0.5001661879472223
2216	92-93	-0.4352856677874044
2216	94-95	-0.5610925715289881
2216	96-97	-0.5248916897962488
2216	98-99	-0.5362368750932021
2216	100	-0.5707527481121097
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1502.0
1	409.0
2	291.0
3	277.0
4	203.0
5	279.0
6	366.0
7	779.0
8	2258.0
9	3085.0
10	3301.0
11	4335.0
12	6056.0
13	9142.0
14	12938.0
15	15184.0
16	16213.0
17	17848.0
18	19122.0
19	21521.0
20	23898.0
21	26842.0
22	29581.0
23	34796.0
24	41746.0
25	50859.0
26	55762.0
27	52065.0
28	53540.0
29	66189.0
30	77839.0
31	86843.0
32	109047.0
33	144477.0
34	208861.0
35	312982.0
36	550906.0
37	1115721.0
38	1468463.0
39	520825.0
40	1374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97554801007986	20.768502180031263	13.286530292846669	23.96941951704221
2	14.490607701001665	21.38078500190822	41.20441932679988	22.924187970290234
3	17.476977552338884	27.12612923866346	31.12072144232691	24.276171766670746
4	11.51586537381487	18.40915552257236	41.30526310081471	28.769716002798063
5	12.263026222677206	39.37422073900378	36.165301906867874	12.197451131451139
6	28.15554853134631	40.245988066075085	19.14791047851909	12.450552924059517
7	25.095626790762964	33.93158099216354	23.997879651432356	16.974912565641134
8	24.10914533576178	36.84561488862428	21.415102598288982	17.630137177324954
9	25.59965932936375	16.09203994165686	21.398403387186356	36.909897341793034
10-11	23.31003838766389	27.433555566830982	29.920523088566625	19.335882956938498
12-13	24.13461704176584	25.501060107073815	28.94409888574991	21.420223965410436
14-15	21.638492255115203	26.856583512767063	26.534663090805942	24.970261141311795
16-17	21.296582664603267	29.457235908834136	27.422277256580287	21.823904169982317
18-19	20.97044679129744	29.464434332093415	28.59446874087673	20.97065013573241
20-21	22.361210126531784	28.509440818460202	27.87117098371749	21.258178071290523
22-23	22.25363917318744	28.433257247119847	27.499579289135163	21.81352429055754
24-25	21.847013091764357	28.65268746456664	27.91330125388708	21.586998189781927
26-27	21.86314636558903	28.81424151844258	27.517976277433043	21.804635838535344
28-29	21.57973746409602	29.016799040436624	27.61338822141187	21.790075274055486
30-31	22.03816563853036	28.65900257737749	27.668519197085324	21.634312587006825
32-33	21.93119174593011	28.919941909463475	27.13665114080196	22.012215203804452
34-35	22.166110276194534	28.886751321687626	27.63324343736688	21.31389496475096
36-37	22.09960315231475	28.633083423232534	27.564741952165235	21.70257147228748
38-39	21.94194971635313	28.632248780289398	27.806351210801246	21.61945029255623
40-41	22.05445032322877	28.308501153382043	27.995541992722277	21.64150653066691
42-43	21.799142555270514	28.527604510155403	27.622590676184362	22.05066225838972
44-45	21.792235782506157	28.928422088708956	27.614014102497308	21.66532802628758
46-47	21.777910162862057	28.55703464469453	27.437876061221818	22.227179131221593
48-49	21.97465497635344	28.368252614273317	27.731663576353398	21.925428833019847
50-51	21.95425393720956	28.44378127838042	27.51254040441896	22.089424379991055
52-53	21.652849789606606	28.65611360129433	27.49395994850497	22.197076660594092
54-55	21.603969052038565	28.49329530559059	27.542779273821438	22.35995636854941
56-57	22.04670489303744	28.375206859363523	27.432495669124187	22.145592578474847
58-59	21.761099568867166	28.292651604457998	27.74743069391506	22.198818132759783
60-61	21.705570726691096	28.567001264535698	27.53574175231119	22.191686256462017
62-63	21.80428539426892	28.283842360419513	27.3791854630414	22.53268678227016
64-65	21.420766667958006	28.17767210320381	27.635387207995972	22.76617402084221
66-67	21.740701971063586	28.080401867935095	27.443442043106316	22.735454117895003
68-69	21.59952218434662	28.036619878452207	27.589670093549636	22.774187843651543
70-71	21.503123759692198	27.853412542573615	27.583322050103735	23.060141647630452
72-73	21.669468668250406	27.899199998605372	27.791087513971675	22.640243819172547
74-75	21.67294013000713	28.075824321848042	27.65705321041064	22.594182337734185
76-77	21.53187638470231	27.97074455000153	27.5796575487607	22.91772151653546
78-79	21.527945595267507	27.913603536737657	27.656197470435252	22.902253397559583
80-81	21.430867508547898	27.940617436175373	27.800288831503106	22.828226223773616
82-83	21.675017932929393	27.651502017224523	28.07936437523572	22.594115674610364
84-85	21.85962894355703	27.750192106425896	27.621940364974122	22.76823858504295
86-87	21.28383014689354	27.9617997187316	27.84980372130042	22.904566413074438
88-89	21.76945018215176	27.429587613370295	27.663214099212663	23.13774810526528
90-91	21.62227024627273	27.40474880251404	27.811774905761354	23.161206045451877
92-93	21.797173668579113	27.38305386500686	27.851752598964403	22.96801986744962
94-95	21.233460884514322	27.41430388054446	28.022329155647206	23.32990607929401
96-97	21.35400012124426	27.279677776914824	27.918514947616085	23.447807154224833
98-99	21.20076464279623	27.498898043849863	27.787193598804276	23.513143714549628
100	21.610074146262736	27.220605305383906	27.42569855462894	23.743621993724414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3995.0
1	2680.0
2	1408.5
3	1364.0
4	1232.0
5	1110.0
6	994.0
7	943.0
8	912.0
9	870.5
10	770.5
11	740.0
12	799.5
13	879.0
14	935.5
15	1010.5
16	1174.5
17	1384.5
18	1816.0
19	2490.0
20	3454.5
21	4874.0
22	7095.0
23	10242.5
24	14682.5
25	20618.0
26	29140.5
27	38473.0
28	48198.0
29	59577.5
30	71151.0
31	84160.0
32	99816.0
33	113331.5
34	125265.0
35	138785.0
36	152349.0
37	168492.0
38	178498.0
39	179047.0
40	178315.0
41	176834.0
42	174620.5
43	178213.0
44	186478.0
45	199387.5
46	208161.5
47	211897.5
48	219021.0
49	213716.5
50	200090.0
51	192688.0
52	183841.0
53	171581.0
54	157780.5
55	139152.0
56	123417.0
57	103817.5
58	83287.0
59	71794.5
60	57704.5
61	43031.0
62	32977.0
63	25242.5
64	17799.5
65	11181.0
66	7493.0
67	5202.0
68	3935.5
69	3015.5
70	2340.5
71	1992.5
72	1654.5
73	1502.0
74	1275.0
75	1021.0
76	816.5
77	650.5
78	489.5
79	363.5
80	275.0
81	202.5
82	165.0
83	115.5
84	85.0
85	67.0
86	48.5
87	33.5
88	34.0
89	34.0
90	23.5
91	16.0
92	11.5
93	11.0
94	9.0
95	9.0
96	12.0
97	11.5
98	8.0
99	5.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.095883585136593
2	0.06246462418182082
3	0.0787387099739247
4	0.08036418346564494
5	0.08086730621308216
6	0.08957519991872633
7	0.08558891968903144
8	0.08671127043331446
9	0.07339786850112962
10-11	0.07908702572215047
12-13	0.07012757064278768
14-15	0.07791629779061386
16-17	0.0789902713476433
18-19	0.07880643803607972
20-21	0.07133700032412715
22-23	0.07442346487090548
24-25	0.07706485929495087
26-27	0.07154018451059219
28-29	0.079851385280757
30-31	0.08392474444750833
32-33	0.08525027937825638
34-35	0.08774654224054104
36-37	0.08775621767799177
38-39	0.08930428767010629
40-41	0.0857534021256936
42-43	0.09114262078574228
44-45	0.08758205980387888
46-47	0.08392474444750833
48-49	0.09323251527509688
50-51	0.08928493679520486
52-53	0.09475155895485925
54-55	0.08663386693370874
56-57	0.09282614690216681
58-59	0.09766386562752467
60-61	0.09599001494855086
62-63	0.09771224281477826
64-65	0.09917323386983634
66-67	0.10126312835919095
68-69	0.09800250593829972
70-71	0.10054714598783797
72-73	0.09765419019007397
74-75	0.10137923360859953
76-77	0.09797347962594757
78-79	0.10552999627495659
80-81	0.10061487404999299
82-83	0.10515265421437865
84-85	0.10105026873527519
86-87	0.09753808494066538
88-89	0.09669632188245311
90-91	0.09741230425380608
92-93	0.09108456816103798
94-95	0.09093943659927724
96-97	0.08889791929717622
98-99	0.10284022466365761
100	0.2420600941420064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5167725.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.69805723381376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.04762099133923	35.17929804635122
2	15.168793402050056	15.68394298934161
3	6.527114006400078	10.12317340423496
4	3.404947596283779	7.041167028432617
5	2.002538167306147	5.1763666393144865
6	1.2774298933433825	3.9624386242950482
7	0.8443579368384767	3.055616546115033
8	0.6020896842330881	2.4901493564296837
9	0.41873236549467546	1.9482884817294557
>10	1.6688464245850794	13.58001844297832
>50	0.031140948968127535	1.0842883657958622
>100	0.006312839424697827	0.5156047519159183
>500	0.0	0.0
>1k	7.57437331692075E-5	0.15964732306578927
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.935087490143148E-5	0.0
5	0.0	0.0	0.0	1.935087490143148E-5	3.870174980286296E-5
6	0.0	0.0	0.0	1.935087490143148E-5	5.8052624704294444E-5
7	0.0	0.0	0.0	1.935087490143148E-5	5.8052624704294444E-5
8	0.0	0.0	0.0	1.935087490143148E-5	5.8052624704294444E-5
9	0.0	0.0	0.0	1.935087490143148E-5	5.8052624704294444E-5
10-11	0.0	0.0	0.0	1.935087490143148E-5	5.8052624704294444E-5
12-13	0.0	0.0	0.0	1.935087490143148E-5	9.675437450715741E-5
14-15	0.0	0.0	0.0	2.9026312352147222E-5	9.675437450715741E-5
16-17	0.0	0.0	0.0	5.8052624704294444E-5	1.1610524940858889E-4
18-19	0.0	0.0	0.0	6.772806215501018E-5	1.1610524940858889E-4
20-21	0.0	0.0	0.0	1.3545612431002037E-4	1.3545612431002037E-4
22-23	0.0	0.0	0.0	2.9993856097218795E-4	1.3545612431002037E-4
24-25	0.0	0.0	0.0	8.998156829165639E-4	1.3545612431002037E-4
26-27	0.0	0.0	0.0	0.0017415787411288334	1.451315617607361E-4
28-29	0.0	0.0	0.0	0.006414815029824536	1.5480699921145184E-4
30-31	0.0	0.0	0.0	0.017928585596176265	1.5480699921145184E-4
32-33	0.0	0.0	0.0	0.04137217053926051	1.5480699921145184E-4
34-35	0.0	0.0	0.0	0.08055769221465925	1.5480699921145184E-4
36-37	0.0	0.0	0.0	0.14040994828478684	1.5480699921145184E-4
38-39	0.0	0.0	0.0	0.23020768326487961	1.5480699921145184E-4
40-41	0.0	0.0	0.0	0.3338896709867495	1.5480699921145184E-4
42-43	0.0	0.0	0.0	0.43744587802176005	1.5480699921145184E-4
44-45	0.0	0.0	0.0	0.5639231963775162	1.7415787411288335E-4
46-47	0.0	0.0	0.0	0.718497985090151	1.9350874901431482E-4
48-49	0.0	0.0	0.0	0.8955294641258968	1.9350874901431482E-4
50-51	0.0	0.0	0.0	1.0801174598106518	2.7091224862004073E-4
52-53	0.0	0.0	0.0	1.265169876493041	2.7091224862004073E-4
54-55	0.0	0.0	0.0	1.4680444489596485	2.7091224862004073E-4
56-57	0.0	0.0	0.0	1.7104722097247822	2.8058768607075647E-4
58-59	0.0	0.0	0.0	1.9884378522463946	2.902631235214722E-4
60-61	0.0	0.0	0.0	2.283151676995196	2.902631235214722E-4
62-63	0.0	0.0	0.0	2.5823645801585804	3.4831574822576664E-4
64-65	0.0	0.0	0.0	2.890391806839567	3.579911856764824E-4
66-67	0.0	0.0	0.0	3.2412908968646743	3.676666231271981E-4
68-69	0.0	0.0	0.0	3.6365228412889614	3.676666231271981E-4
70-71	0.0	0.0	0.0	4.070263026767098	3.676666231271981E-4
72-73	0.0	0.0	0.0	4.5136883251333995	4.1604381038077686E-4
74-75	0.0	0.0	0.0	4.950814913719286	4.934473099865028E-4
76-77	0.0	0.0	0.0	5.427494690603699	5.031227474372185E-4
78-79	0.0	0.0	0.0	5.965197451489775	5.418244972400815E-4
80-81	0.0	0.0	0.0	6.553096381870166	5.418244972400815E-4
82-83	0.0	0.0	0.0	7.166228853122021	5.418244972400815E-4
84-85	0.0	0.0	0.0	7.760542598532236	5.418244972400815E-4
86-87	0.0	0.0	0.0	8.375435999400123	5.514999346907972E-4
88	0.0	0.0	0.0	8.857011547634597	5.611753721415129E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8700	0.0	28.215797	1
GTATCAA	12175	0.0	25.145082	1
GTACATG	16045	0.0	25.117832	1
GTACTAG	2255	0.0	24.608011	1
TACATGG	15745	0.0	24.155289	2
ACATGGG	15470	0.0	23.490679	3
ATCAACG	12955	0.0	22.17227	3
CAACGCA	13010	0.0	22.00435	5
CGACGAG	1030	0.0	21.671041	24-25
CTGTGCG	2605	0.0	21.645597	9
AACGCAG	13765	0.0	21.575981	6
GTGATCG	1400	0.0	21.146477	8
TATCAAC	14380	0.0	20.955862	2
GCATCGT	2230	0.0	20.229813	8
CATGGGG	10230	0.0	20.17298	4
CTAGTAC	3215	0.0	19.886772	3
TCAACGC	14765	0.0	19.644133	4
ACGCAGA	14625	0.0	19.600912	7
CGCAGAG	14645	0.0	19.573385	8
CAATGCG	1130	0.0	19.545134	18-19
>>END_MODULE