FastQCFastQC Report
Mon 16 Jan 2017
ERR1146519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5167725
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214760.41557938938314254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200580.38813984877291263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117000.22640523634674833No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143950.050.931141
GTATCAA245500.038.522061
ATCAACG302100.030.7117313
CAACGCA305200.030.3525125
TATCAAC310900.030.281662
AACGCAG320850.029.1638586
TCAACGC321400.028.911114
ACGCAGA349150.026.517087
GTACTAG22250.026.4197651
CGCAGAG351750.026.2676338
CTGTGCG25050.025.7030339
GCAGAGT375150.024.103039
GAGTACT260700.023.11098912-13
CTAGTAC32850.022.7609123
GTACATG150850.022.6952511
CGACGAG11450.022.5750524-25
TACATGG146900.022.248432
TAGTACT32350.022.0949524
ACATGGG145700.021.8502833
CAGAGTA364000.021.53605810-11
Produced by FastQC (version 0.11.5)