##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5167725
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.349892264003984	33.0	31.0	34.0	30.0	34.0
2	32.406415395556074	34.0	31.0	34.0	30.0	34.0
3	32.50904527620955	34.0	31.0	34.0	30.0	34.0
4	36.027385551669255	37.0	35.0	37.0	35.0	37.0
5	36.07684193721609	37.0	35.0	37.0	35.0	37.0
6	36.14368663193185	37.0	36.0	37.0	35.0	37.0
7	36.110591604622925	37.0	36.0	37.0	35.0	37.0
8	36.11134067699036	37.0	36.0	37.0	35.0	37.0
9	37.89104915605997	39.0	38.0	39.0	35.0	39.0
10-11	37.84293610824879	39.0	38.0	39.0	35.0	39.0
12-13	37.81612121388038	39.0	38.0	39.0	35.0	39.0
14-15	39.141914382054	40.0	39.0	41.0	36.0	41.0
16-17	39.093900120459196	40.0	39.0	41.0	36.0	41.0
18-19	39.22164047041977	40.0	39.0	41.0	36.0	41.0
20-21	39.290189106424975	41.0	39.0	41.0	36.0	41.0
22-23	39.249745584372235	41.0	39.0	41.0	36.0	41.0
24-25	39.23956537934971	41.0	39.0	41.0	36.0	41.0
26-27	39.15420228437078	41.0	39.0	41.0	35.5	41.0
28-29	39.08930980266945	40.0	39.0	41.0	35.0	41.0
30-31	38.9241426352989	40.0	38.5	41.0	35.0	41.0
32-33	38.78716379064288	40.0	38.0	41.0	35.0	41.0
34-35	38.70120004450701	40.0	38.0	41.0	35.0	41.0
36-37	38.620120942968136	40.0	38.0	41.0	35.0	41.0
38-39	38.52226792253845	40.0	38.0	41.0	35.0	41.0
40-41	38.41516112022215	40.0	38.0	41.0	34.0	41.0
42-43	38.29685732116164	40.0	38.0	41.0	34.0	41.0
44-45	38.12862884538167	40.0	38.0	41.0	33.5	41.0
46-47	37.85520833635691	40.0	38.0	41.0	33.0	41.0
48-49	37.54258401907996	40.0	37.0	41.0	32.5	41.0
50-51	37.42525705218447	40.0	37.0	41.0	32.0	41.0
52-53	37.32886928000232	40.0	37.0	41.0	32.0	41.0
54-55	37.185739372741395	40.0	36.5	41.0	31.5	41.0
56-57	37.04936340459293	40.0	36.0	41.0	31.0	41.0
58-59	36.88864190722223	39.5	35.5	41.0	31.0	41.0
60-61	36.71003217082952	39.0	35.0	41.0	31.0	41.0
62-63	36.49755066300935	39.0	35.0	41.0	31.0	41.0
64-65	36.25241774668737	38.5	35.0	41.0	31.0	41.0
66-67	36.01110014561533	38.0	35.0	40.5	30.0	41.0
68-69	35.74123129616998	37.0	35.0	40.0	30.0	41.0
70-71	35.45117358218559	37.0	35.0	39.5	30.0	41.0
72-73	35.136869705721566	36.0	35.0	39.0	29.5	41.0
74-75	34.814051734564046	36.0	35.0	39.0	29.0	41.0
76-77	34.503716335524814	35.5	34.5	38.5	29.0	40.0
78-79	34.199559090315375	35.0	34.0	37.0	29.0	39.0
80-81	33.92338853170399	35.0	34.0	37.0	29.0	39.0
82-83	33.64867044976271	35.0	34.0	36.5	29.0	39.0
84-85	33.41826829020508	35.0	34.0	36.0	29.0	37.0
86-87	33.20934685959489	35.0	34.0	36.0	29.0	37.0
88-89	32.997091660256686	35.0	34.0	35.0	29.0	36.5
90-91	32.89274361542071	35.0	34.0	35.0	29.0	36.0
92-93	32.77948826611323	35.0	34.0	35.0	28.0	36.0
94-95	32.699788785200454	35.0	34.0	35.0	27.5	36.0
96-97	32.63867359427988	35.0	34.0	35.0	27.0	36.0
98-99	32.56437368474522	35.0	34.0	35.0	27.0	35.0
100	31.85116293920439	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31143845019180105
1101	2	0.3265860837869212
1101	3	0.32753450110172366
1101	4	0.228203648034075
1101	5	0.2304504092891193
1101	6	0.25202424536299617
1101	7	0.2542226514551018
1101	8	0.2528338942862547
1101	9	0.3226608587837063
1101	10-11	0.3329039356300356
1101	12-13	0.3573483806161022
1101	14-15	0.5031335857529555
1101	16-17	0.5361802171703971
1101	18-19	0.47073904973909464
1101	20-21	0.40784258596155354
1101	22-23	0.4135847085849491
1101	24-25	0.4630630175281212
1101	26-27	0.44219069406356226
1101	28-29	0.47511385636965997
1101	30-31	0.5093687044420463
1101	32-33	0.47283979465306203
1101	34-35	0.47700685230617523
1101	36-37	0.4965041004262929
1101	38-39	0.5238322055189641
1101	40-41	0.4937947070416868
1101	42-43	0.5197068058619081
1101	44-45	0.49459799547639705
1101	46-47	0.5229943596050148
1101	48-49	0.561329310155628
1101	50-51	0.5336677608248337
1101	52-53	0.4903682776325269
1101	54-55	0.5841317733644757
1101	56-57	0.5163727994910019
1101	58-59	0.4844519129516698
1101	60-61	0.4783949553772615
1101	62-63	0.4812129341831195
1101	64-65	0.4758769913614742
1101	66-67	0.484686891111032
1101	68-69	0.44899042192518124
1101	70-71	0.49401006555862637
1101	72-73	0.42501558191651867
1101	74-75	0.4176837369484474
1101	76-77	0.3560795839955233
1101	78-79	0.37456438250653434
1101	80-81	0.38354607602693136
1101	82-83	0.38805562161923035
1101	84-85	0.42153129821308966
1101	86-87	0.39837996861523095
1101	88-89	0.4421205732691149
1101	90-91	0.3987362411598028
1101	92-93	0.3476131851803146
1101	94-95	0.4588186433059036
1101	96-97	0.38705109240653
1101	98-99	0.44110607679651537
1101	100	0.5141659204171098
1102	1	0.29494379499209344
1102	2	0.24810303624827412
1102	3	0.2456006647697464
1102	4	0.16165566090802486
1102	5	0.18510915460647936
1102	6	0.1868736939990967
1102	7	0.15970305033654597
1102	8	0.18457196213831395
1102	9	0.20629500641204856
1102	10-11	0.21865194386091957
1102	12-13	0.2401405823783591
1102	14-15	0.3978953965480798
1102	16-17	0.4674062710932674
1102	18-19	0.3724109961327997
1102	20-21	0.3346646088603009
1102	22-23	0.3391853628349324
1102	24-25	0.33451683486574524
1102	26-27	0.29220868593819915
1102	28-29	0.283146283953684
1102	30-31	0.37241921887247287
1102	32-33	0.33000308278686674
1102	34-35	0.2983038184856639
1102	36-37	0.3367420152682712
1102	38-39	0.3886907925500438
1102	40-41	0.3459303161800804
1102	42-43	0.34014397148212794
1102	44-45	0.3623736088357603
1102	46-47	0.41945467723283514
1102	48-49	0.4628397939840454
1102	50-51	0.4685185074084899
1102	52-53	0.4477808666685661
1102	54-55	0.4429906453108643
1102	56-57	0.4096928332587453
1102	58-59	0.4494938377129216
1102	60-61	0.41734951666042974
1102	62-63	0.4359122007899856
1102	64-65	0.4141444318722165
1102	66-67	0.40190929389687113
1102	68-69	0.30247927329435953
1102	70-71	0.3496984420689415
1102	72-73	0.4001291892610084
1102	74-75	0.3936135739135693
1102	76-77	0.3234982934346675
1102	78-79	0.3700474405976024
1102	80-81	0.3185201276282612
1102	82-83	0.34183110834955954
1102	84-85	0.30578207645400113
1102	86-87	0.3747630952970269
1102	88-89	0.4292218659404696
1102	90-91	0.3402720718990011
1102	92-93	0.3139400358108233
1102	94-95	0.31318857946376966
1102	96-97	0.3341101226392098
1102	98-99	0.3644805188373539
1102	100	0.35023457445604933
1103	1	0.24533074050893333
1103	2	0.22803285075692514
1103	3	0.22934915196564276
1103	4	0.18293702204261564
1103	5	0.1759950356180937
1103	6	0.15408930319535585
1103	7	0.19019191138266933
1103	8	0.19162451129454894
1103	9	0.2372591218657618
1103	10-11	0.23183493922589804
1103	12-13	0.2091521737956441
1103	14-15	0.332764543913747
1103	16-17	0.3781410183854632
1103	18-19	0.31092393673556984
1103	20-21	0.2922588854695505
1103	22-23	0.31062196794133
1103	24-25	0.27761848336744066
1103	26-27	0.3114775711162494
1103	28-29	0.3422280749513007
1103	30-31	0.3693358665673614
1103	32-33	0.3091778419834341
1103	34-35	0.27476976430492783
1103	36-37	0.30823791840040826
1103	38-39	0.29921409120432685
1103	40-41	0.32249907010686485
1103	42-43	0.32549992828996466
1103	44-45	0.33195189834919603
1103	46-47	0.2959595355685636
1103	48-49	0.32575306620823596
1103	50-51	0.28983438032556563
1103	52-53	0.22537183195341015
1103	54-55	0.27405192706483916
1103	56-57	0.25318722447769915
1103	58-59	0.23246179962361424
1103	60-61	0.2175712668281946
1103	62-63	0.2586510087745282
1103	64-65	0.23946585327779246
1103	66-67	0.25988483484942293
1103	68-69	0.25253580117822594
1103	70-71	0.25968821388466523
1103	72-73	0.2756621256055922
1103	74-75	0.26631062672296224
1103	76-77	0.20238551521980241
1103	78-79	0.2107376381242574
1103	80-81	0.2626539285057419
1103	82-83	0.20632967040351247
1103	84-85	0.19241364733619548
1103	86-87	0.1718951945573579
1103	88-89	0.21200969611258103
1103	90-91	0.11601129990086889
1103	92-93	0.1278658145729139
1103	94-95	0.18965377110826864
1103	96-97	0.14878973428728415
1103	98-99	0.1617910923093433
1103	100	0.17955051143654188
1104	1	0.16943312868404092
1104	2	0.11949133138485735
1104	3	0.1409057748144633
1104	4	0.10461360122451424
1104	5	0.06986198849581626
1104	6	0.07540310715161525
1104	7	0.11343920725702361
1104	8	0.09674490685092962
1104	9	0.20387642912123027
1104	10-11	0.18517567743836594
1104	12-13	0.18283416529212815
1104	14-15	0.2820327727448202
1104	16-17	0.30454488827165704
1104	18-19	0.25810667723354186
1104	20-21	0.21513955565851717
1104	22-23	0.2615725380996068
1104	24-25	0.2607527440617545
1104	26-27	0.27801183227494164
1104	28-29	0.2827341569363
1104	30-31	0.31372302816709663
1104	32-33	0.30306088359122185
1104	34-35	0.23140059460722995
1104	36-37	0.22764911151276834
1104	38-39	0.216321587140051
1104	40-41	0.25907768314417723
1104	42-43	0.2602036770124414
1104	44-45	0.2143278796843262
1104	46-47	0.2727534628324122
1104	48-49	0.44560859076661785
1104	50-51	0.3335651739807801
1104	52-53	0.32063863979070106
1104	54-55	0.35340046023316063
1104	56-57	0.3017876307302245
1104	58-59	0.3105504101184522
1104	60-61	0.2568964089372443
1104	62-63	0.2259531657672511
1104	64-65	0.22153932198477833
1104	66-67	0.20813567159883206
1104	68-69	0.232589239367897
1104	70-71	0.16000055582716755
1104	72-73	0.10352989578693439
1104	74-75	0.10827245338106195
1104	76-77	0.14150220040350803
1104	78-79	0.20011407454891383
1104	80-81	0.18529394496016494
1104	82-83	0.15959181279932722
1104	84-85	0.1822396007506626
1104	86-87	0.18335858403774807
1104	88-89	0.22144119411169783
1104	90-91	0.15992145919380363
1104	92-93	0.1659732245988934
1104	94-95	0.22995197663922795
1104	96-97	0.2258967194082544
1104	98-99	0.21110297258366018
1104	100	0.271152271735172
1105	1	0.2204367619668659
1105	2	0.20125974626338916
1105	3	0.19232541626539046
1105	4	0.1619635314372445
1105	5	0.11861830872435064
1105	6	0.19860968290475256
1105	7	0.19401010515037598
1105	8	0.16374313094138415
1105	9	0.1831425494377399
1105	10-11	0.199919664056587
1105	12-13	0.2349337574303405
1105	14-15	0.3601104728605051
1105	16-17	0.3767482380606708
1105	18-19	0.3305336449901972
1105	20-21	0.2821402236611945
1105	22-23	0.31966528185269993
1105	24-25	0.26995855405572655
1105	26-27	0.2475908276640979
1105	28-29	0.28356047081065583
1105	30-31	0.3131914772604105
1105	32-33	0.31052554190514314
1105	34-35	0.29004077469640777
1105	36-37	0.26796877523044316
1105	38-39	0.2730583669327018
1105	40-41	0.2752191802304864
1105	42-43	0.29759824935495516
1105	44-45	0.2957164019863896
1105	46-47	0.33313425151003884
1105	48-49	0.4052623155603783
1105	50-51	0.361147981872989
1105	52-53	0.320730286498744
1105	54-55	0.312028828079967
1105	56-57	0.3376987483584344
1105	58-59	0.297851706478113
1105	60-61	0.29006260582967514
1105	62-63	0.3287572834877892
1105	64-65	0.272862428903224
1105	66-67	0.2612993159607342
1105	68-69	0.2599146218401671
1105	70-71	0.26742448227084026
1105	72-73	0.2721853639047467
1105	74-75	0.25835446021315533
1105	76-77	0.20797824191743075
1105	78-79	0.1572610242749235
1105	80-81	0.20651837213429758
1105	82-83	0.19350258827982003
1105	84-85	0.17536745537589837
1105	86-87	0.2234483842343451
1105	88-89	0.2679863168775469
1105	90-91	0.17234712353060644
1105	92-93	0.21829996480015978
1105	94-95	0.24770414764791582
1105	96-97	0.23966463464251575
1105	98-99	0.21723841419859014
1105	100	0.2157648030308188
1106	1	0.11044256432584376
1106	2	0.08849398027000177
1106	3	0.10255241749190702
1106	4	0.10670663409910475
1106	5	0.06240581738467199
1106	6	0.09761840594693894
1106	7	0.0820129633330069
1106	8	0.11496421943555646
1106	9	0.14097008240679543
1106	10-11	0.13131667617416554
1106	12-13	0.1535702784263151
1106	14-15	0.21928912656250787
1106	16-17	0.266681610667419
1106	18-19	0.17574873786118417
1106	20-21	0.1879110663862349
1106	22-23	0.2010331491954318
1106	24-25	0.1580230706137229
1106	26-27	0.11399162306157251
1106	28-29	0.15801923128670836
1106	30-31	0.19609836602133868
1106	32-33	0.19826461217131452
1106	34-35	0.1698280865463886
1106	36-37	0.16781451847539586
1106	38-39	0.12095974367424134
1106	40-41	0.12473883444712897
1106	42-43	0.14886831880152585
1106	44-45	0.15155406714195863
1106	46-47	0.14542674182133908
1106	48-49	0.19730860677142203
1106	50-51	0.13740551795821432
1106	52-53	0.1147313831401604
1106	54-55	0.11092460680840333
1106	56-57	0.07695833366486227
1106	58-59	0.1138363854135136
1106	60-61	0.09875009804481039
1106	62-63	0.02603629184734757
1106	64-65	0.05063691173307916
1106	66-67	0.05462239210638842
1106	68-69	0.06010176897229513
1106	70-71	0.047254392035071646
1106	72-73	0.04161863433855473
1106	74-75	0.05106183913822804
1106	76-77	0.031221720690943755
1106	78-79	0.03059464129883338
1106	80-81	0.04556385970310828
1106	82-83	0.02668813654955926
1106	84-85	-0.007718867679415098
1106	86-87	0.005276849836199915
1106	88-89	0.07256198003753411
1106	90-91	0.05248434998528495
1106	92-93	0.10408511616903127
1106	94-95	0.1024082916137985
1106	96-97	0.09683954628841462
1106	98-99	0.12452691289472284
1106	100	0.11992889321073719
1107	1	0.050627647900547856
1107	2	0.04048248812007671
1107	3	0.06244768521351318
1107	4	0.047645984452991286
1107	5	0.03302660519826617
1107	6	0.06758496473160847
1107	7	0.05303656363943787
1107	8	0.07615099752200649
1107	9	0.10392462335397568
1107	10-11	0.09431651399486896
1107	12-13	0.0892564105858753
1107	14-15	0.1790633049921837
1107	16-17	0.2513972298381617
1107	18-19	0.18716906185402138
1107	20-21	0.1544417508540974
1107	22-23	0.1639687592089274
1107	24-25	0.16471372619324853
1107	26-27	0.16326560673014257
1107	28-29	0.14093247103225792
1107	30-31	0.18912790179792438
1107	32-33	0.13939548691008952
1107	34-35	0.0970848019409587
1107	36-37	0.04960001545705239
1107	38-39	0.04730755102996653
1107	40-41	0.09583210574080425
1107	42-43	0.08041639783547083
1107	44-45	0.13450222394297384
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2216	52-53	-0.4333433601460328
2216	54-55	-0.40720549694253094
2216	56-57	-0.4040775272455406
2216	58-59	-0.4411764898558488
2216	60-61	-0.5118771053901554
2216	62-63	-0.5138888251675056
2216	64-65	-0.4391443479790951
2216	66-67	-0.4415442782520671
2216	68-69	-0.4373543512209892
2216	70-71	-0.4124763939576894
2216	72-73	-0.3649682730161814
2216	74-75	-0.3824723295531456
2216	76-77	-0.41388033632171783
2216	78-79	-0.42001126212326056
2216	80-81	-0.4499410276537432
2216	82-83	-0.37265011442232066
2216	84-85	-0.36382457466685025
2216	86-87	-0.28686545800402286
2216	88-89	-0.21255954513797093
2216	90-91	-0.2813558110435679
2216	92-93	-0.2818113712585344
2216	94-95	-0.3010313669337066
2216	96-97	-0.30359082295846207
2216	98-99	-0.30168567194215257
2216	100	-0.343802854367393
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	45.0
8	173.0
9	445.0
10	537.0
11	371.0
12	391.0
13	483.0
14	906.0
15	1620.0
16	3075.0
17	5658.0
18	9592.0
19	14436.0
20	20282.0
21	29976.0
22	42595.0
23	47352.0
24	42193.0
25	34504.0
26	30696.0
27	33274.0
28	40324.0
29	50274.0
30	61760.0
31	77630.0
32	98620.0
33	129484.0
34	184963.0
35	284390.0
36	491995.0
37	1105571.0
38	1652819.0
39	668729.0
40	2558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0797235085465	20.533031710984382	13.829971689040002	23.557273091429114
2	14.862009723005423	21.437442743526994	40.980095356821764	22.72045217664582
3	17.37312963792902	27.291534596715454	31.81515779891929	23.520177966436236
4	11.420214275674418	18.720642029365425	40.7416707678928	29.117472927067357
5	11.731513846533726	39.001465087058605	36.288451278550184	12.978569787857483
6	27.667668516130707	40.54421113111456	18.656422528511683	13.131697824243055
7	24.210348695092847	34.612297405976015	23.94015237655881	17.237201522372324
8	24.563109106956293	35.798416667811594	21.208721311513475	18.429752913718634
9	25.90678217335136	14.820199375972864	21.609571254192367	37.6634471964834
10-11	23.471577071554083	26.846846689947725	29.74147885900563	19.940097379492563
12-13	24.96479592083557	25.02753145726601	29.33171753527906	20.675955086619354
14-15	21.965073796019894	25.959268920005314	27.997400016196682	24.07825726777811
16-17	20.51843252874773	28.692214272002776	28.754011446458318	22.035341752791176
18-19	20.096862795638	27.561041402768954	31.72138516739097	20.620710634202073
20-21	21.384011754531844	26.592993142609227	31.504008836410875	20.518986266448053
22-23	21.267627437605523	26.54706858433837	31.185763561335015	20.99954041672109
24-25	20.85707927569675	26.769168251019547	31.565282595339344	20.808469877944358
26-27	20.967302638179905	26.94488207108351	31.149287484273742	20.938527806462844
28-29	20.630732091974707	27.1415951893725	31.294486451968712	20.933186266684082
30-31	21.159839727972564	26.742733827762137	31.279333569930536	20.818092874334766
32-33	21.133859078321763	26.989223185607486	30.705392103220873	21.17152563284988
34-35	21.30302557085631	27.015903922020527	31.121806352086868	20.559264155036296
36-37	21.335453546244764	26.774447919004903	30.915094084044803	20.975004450705534
38-39	21.23956773256396	26.746661292237395	31.104941569046307	20.908829406152336
40-41	21.350985696036346	26.61289799791725	31.044275361919198	20.9918409441272
42-43	21.26951414790841	26.7955047917604	30.509131194094113	21.425849866237076
44-45	21.312612834277065	27.224168660703153	30.25126939922312	21.21194910579667
46-47	21.525042450981815	27.037110140342218	29.638167665655583	21.79967974302038
48-49	21.96814186212029	27.087331710181004	29.414662785652943	21.52986364204576
50-51	21.806210663299613	27.38158473990005	29.02115534398599	21.791049252814343
52-53	21.48243964026968	27.643966484538733	28.985626016348263	21.88796785884332
54-55	21.46509703387703	27.573397903576357	28.82802628748871	22.133478775057906
56-57	21.887386087303323	27.354305386409884	28.729883409864527	22.02842511642227
58-59	21.51086224864623	27.306070805503772	29.10191136603259	22.08115557981741
60-61	21.503134331722976	27.648686642006382	28.893237276201923	21.95494175006872
62-63	21.569719070200183	27.35720984586126	28.730408771614606	22.34266231232395
64-65	21.183182176701933	27.264059811428975	28.94495973843723	22.607798273431857
66-67	21.50164948804721	27.234404171788256	28.79467395713034	22.46927238303419
68-69	21.40539332387374	27.100550207857914	28.939143324478465	22.55491314378988
70-71	21.364920648389944	26.941395792213257	28.858798052496365	22.83488550690043
72-73	21.519152319387906	26.950758864024692	29.02235571743296	22.507733099154446
74-75	21.555101958671596	27.25038431886158	28.728028417148128	22.466485305318695
76-77	21.474675611307543	27.156758340462844	28.54974630811986	22.818819740109753
78-79	21.32863448705858	27.167011939562872	28.664829661984754	22.83952391139379
80-81	21.228706283672135	27.258424165817313	28.70234572184757	22.810523828662976
82-83	21.49409948897844	26.949566227682475	28.96329074046266	22.59304354287643
84-85	21.683434938897335	27.02969563225081	28.455282586282166	22.83158684256969
86-87	21.0537035155702	27.255426710980167	28.646938449704656	23.043931323744975
88-89	21.463535695107616	26.849455030985585	28.446714947099544	23.240294326807252
90-91	21.234527766086625	26.867267511332354	28.57445007232389	23.323754650257126
92-93	21.40520109146717	26.940404893992962	28.523094213834604	23.131299800705264
94-95	20.875542453715585	26.901050404801186	28.671511354519456	23.551895786963772
96-97	20.980928196884207	26.753681395473716	28.603244281918045	23.662146125724025
98-99	20.83351273226033	26.902137393486626	28.489084982263453	23.77526489198959
100	21.147234376131664	26.648601376256764	28.28962121760806	23.914543030003514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	341.0
1	245.0
2	590.5
3	971.0
4	581.0
5	413.5
6	960.0
7	2415.5
8	4425.5
9	5478.0
10	4164.0
11	2517.5
12	2558.0
13	3062.0
14	3619.5
15	4333.5
16	5190.5
17	6104.5
18	7172.0
19	8400.0
20	9780.0
21	11645.0
22	14121.0
23	17218.0
24	21512.0
25	27315.5
26	35401.0
27	44206.5
28	53787.5
29	64459.5
30	74525.0
31	85973.0
32	100904.0
33	113099.5
34	123664.5
35	136136.0
36	148694.5
37	165202.0
38	174978.5
39	174077.0
40	173796.5
41	172918.0
42	170329.5
43	173542.0
44	181235.5
45	194830.0
46	203991.0
47	208421.0
48	215797.0
49	210049.0
50	197018.5
51	188374.0
52	178342.5
53	166416.0
54	153776.5
55	135923.5
56	119970.0
57	101142.0
58	80979.0
59	70152.0
60	56729.5
61	41762.5
62	31465.0
63	23875.0
64	16766.0
65	10482.5
66	7009.0
67	4754.5
68	3547.0
69	2744.5
70	2042.5
71	1690.5
72	1475.5
73	1456.5
74	1228.5
75	911.5
76	720.5
77	547.5
78	434.5
79	312.5
80	214.0
81	155.0
82	115.5
83	81.5
84	48.0
85	31.5
86	19.5
87	8.5
88	6.0
89	4.5
90	3.5
91	1.5
92	0.5
93	1.5
94	1.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0022253506136646205
2	0.003618613606567687
3	1.7415787411288335E-4
4	0.05037032736842614
5	0.002205999738763189
6	0.005456946722203678
7	1.935087490143148E-5
8	3.096139984229037E-4
9	1.935087490143148E-5
10-11	8.707893705644167E-5
12-13	0.0
14-15	9.67543745071574E-6
16-17	2.5156137371860925E-4
18-19	5.805262470429445E-5
20-21	3.096139984229037E-4
22-23	0.0
24-25	0.0
26-27	2.8058768607075647E-4
28-29	0.0
30-31	2.1285962391574627E-4
32-33	2.0318418646503053E-4
34-35	1.161052494085889E-4
36-37	9.675437450715741E-5
38-39	8.611139331137008E-4
40-41	6.095525593950916E-4
42-43	0.0
44-45	4.740964350850713E-4
46-47	0.0
48-49	5.805262470429445E-5
50-51	0.0
52-53	3.9669293547934533E-4
54-55	3.773420605779139E-4
56-57	1.2578068685930463E-4
58-59	6.482543091979546E-4
60-61	7.06306933902249E-4
62-63	2.0318418646503053E-4
64-65	3.2896487332433516E-4
66-67	0.0011804033689873203
68-69	0.0025639909244396714
70-71	0.003608938169116971
72-73	0.00320256979618691
74-75	0.002728473361101839
76-77	0.004566806476737829
78-79	0.0014222893052552139
80-81	0.0011997542438887518
82-83	7.159823713529648E-4
84-85	5.611753721415129E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	3.870174980286296E-5
94-95	5.805262470429445E-5
96-97	3.386403107750509E-4
98-99	1.0642981195787314E-4
100	0.07475242974422981
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5167725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.04635989299851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.34643493828648	31.877046906623224
2	15.980837872003997	15.35642175579929
3	6.866385443337003	9.897144785238474
4	3.5195938382818537	6.764146889250797
5	2.10023291531323	5.045427325413046
6	1.363421783188195	3.9304472208608274
7	0.8889215661260217	2.98966118379171
8	0.6182585699483654	2.3764059006935803
9	0.4740344318644718	2.0498066023530144
>10	1.7820380965626583	13.53033480295264
>50	0.042008714230280284	1.3418524281151807
>100	0.01476179086460076	1.3139859872152062
>500	0.0017318174318334163	0.5794647586595097
>1k	0.001102065638415101	0.9344583115959291
>5k	0.0	0.0
>10k+	2.3615692251752164E-4	2.013395141437556
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21476	0.41557938938314254	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20058	0.38813984877291263	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11700	0.22640523634674833	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.870174980286296E-5
2	0.0	0.0	0.0	0.0	5.8052624704294444E-5
3	0.0	0.0	0.0	0.0	5.8052624704294444E-5
4	0.0	0.0	0.0	0.0	5.8052624704294444E-5
5	0.0	0.0	0.0	0.0	7.740349960572592E-5
6	0.0	0.0	0.0	0.0	7.740349960572592E-5
7	0.0	0.0	0.0	0.0	7.740349960572592E-5
8	0.0	0.0	0.0	0.0	7.740349960572592E-5
9	0.0	0.0	0.0	0.0	7.740349960572592E-5
10-11	0.0	0.0	0.0	0.0	7.740349960572592E-5
12-13	0.0	0.0	0.0	0.0	1.7415787411288335E-4
14-15	0.0	0.0	0.0	0.0	2.61236811169325E-4
16-17	0.0	0.0	0.0	1.935087490143148E-5	3.2896487332433516E-4
18-19	0.0	0.0	0.0	2.9026312352147222E-5	3.2896487332433516E-4
20-21	0.0	0.0	0.0	7.740349960572592E-5	3.6766662312719817E-4
22-23	0.0	0.0	0.0	2.4188593626789352E-4	3.8701749802862965E-4
24-25	0.0	0.0	0.0	8.611139331137009E-4	3.8701749802862965E-4
26-27	0.0	0.0	0.0	0.0016835261164245387	4.1604381038077686E-4
28-29	0.0	0.0	0.0	0.006318060655317378	4.257192478314926E-4
30-31	0.0	0.0	0.0	0.017870532971471974	4.257192478314926E-4
32-33	0.0	0.0	0.0	0.04137217053926051	4.257192478314926E-4
34-35	0.0	0.0	0.0	0.08079957815092714	4.450701227329241E-4
36-37	0.0	0.0	0.0	0.1410195008441819	4.450701227329241E-4
38-39	0.0	0.0	0.0	0.23104944632309188	4.6442099763435555E-4
40-41	0.0	0.0	0.0	0.3351087761055397	4.6442099763435555E-4
42-43	0.0	0.0	0.0	0.4387230357652545	4.6442099763435555E-4
44-45	0.0	0.0	0.0	0.5656163979313915	4.6442099763435555E-4
46-47	0.0	0.0	0.0	0.7208007392034212	4.740964350850713E-4
48-49	0.0	0.0	0.0	0.8980934550503364	5.031227474372185E-4
50-51	0.0	0.0	0.0	1.0838618541040788	5.031227474372185E-4
52-53	0.0	0.0	0.0	1.2703946127164274	5.2247362233865E-4
54-55	0.0	0.0	0.0	1.4749914130492625	5.2247362233865E-4
56-57	0.0	0.0	0.0	1.7202540769874557	5.2247362233865E-4
58-59	0.0	0.0	0.0	2.0012191051187904	5.418244972400815E-4
60-61	0.0	0.0	0.0	2.298990368102018	5.418244972400815E-4
62-63	0.0	0.0	0.0	2.601173630562772	5.611753721415129E-4
64-65	0.0	0.0	0.0	2.9126840147260156	5.611753721415129E-4
66-67	0.0	0.0	0.0	3.2680531568533544	5.708508095922287E-4
68-69	0.0	0.0	0.0	3.667987363878689	5.902016844936602E-4
70-71	0.0	0.0	0.0	4.106865206643155	6.095525593950916E-4
72-73	0.0	0.0	0.0	4.555457188608139	6.482543091979546E-4
74-75	0.0	0.0	0.0	4.997082855608609	6.772806215501018E-4
76-77	0.0	0.0	0.0	5.47766183378566	6.772806215501018E-4
78-79	0.0	0.0	0.0	6.018702620592234	6.772806215501018E-4
80-81	0.0	0.0	0.0	6.609581585707444	6.869560590008175E-4
82-83	0.0	0.0	0.0	7.22527804788374	7.159823713529648E-4
84-85	0.0	0.0	0.0	7.821052784349012	7.159823713529648E-4
86-87	0.0	0.0	0.0	8.439313237449749	7.256578088036805E-4
88	0.0	0.0	0.0	8.92247555742614	7.353332462543962E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14395	0.0	50.93114	1
GTATCAA	24550	0.0	38.52206	1
ATCAACG	30210	0.0	30.711731	3
CAACGCA	30520	0.0	30.352512	5
TATCAAC	31090	0.0	30.28166	2
AACGCAG	32085	0.0	29.163858	6
TCAACGC	32140	0.0	28.91111	4
ACGCAGA	34915	0.0	26.51708	7
GTACTAG	2225	0.0	26.419765	1
CGCAGAG	35175	0.0	26.267633	8
CTGTGCG	2505	0.0	25.703033	9
GCAGAGT	37515	0.0	24.10303	9
GAGTACT	26070	0.0	23.110989	12-13
CTAGTAC	3285	0.0	22.760912	3
GTACATG	15085	0.0	22.695251	1
CGACGAG	1145	0.0	22.57505	24-25
TACATGG	14690	0.0	22.24843	2
TAGTACT	3235	0.0	22.094952	4
ACATGGG	14570	0.0	21.850283	3
CAGAGTA	36400	0.0	21.536058	10-11
>>END_MODULE