FastQCFastQC Report
Mon 16 Jan 2017
ERR1146518_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146518_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381691
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.035.4565961
GTATCAA59050.032.4943731
CAACGCA62750.028.5438065
ATCAACG63700.028.0451453
GTACATG97150.026.1407991
TATCAAC72850.026.0069272
AACGCAG71000.025.8800286
TACATGG97400.025.3404252
TCAACGC71800.025.0122324
GTACTAG12700.024.81071
ACATGGG98400.023.84073
ACGCAGA76350.023.6346537
CGCAGAG77700.023.102038
CTGTGCG16350.020.9788069
CATGGGG72750.020.8729134
CTAGTAC18000.019.8497313
GCATCGT14050.019.0636628
CTCGTCG9000.018.794719
GTGATCG7550.018.6716238
TAGTACT20750.018.578444
Produced by FastQC (version 0.11.5)