##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146518_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3381691
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03069588557914	31.0	31.0	34.0	28.0	34.0
2	31.201126596131935	31.0	31.0	34.0	28.0	34.0
3	31.310010583462535	31.0	31.0	34.0	28.0	34.0
4	34.851208759168124	37.0	35.0	37.0	32.0	37.0
5	34.90364643014397	37.0	35.0	37.0	32.0	37.0
6	35.097993873479275	37.0	35.0	37.0	32.0	37.0
7	35.05168094896902	37.0	35.0	37.0	32.0	37.0
8	34.99791613130827	37.0	35.0	37.0	32.0	37.0
9	36.630140068977326	39.0	37.0	39.0	32.0	39.0
10-11	36.620846345807465	39.0	37.0	39.0	32.0	39.0
12-13	36.56659360657139	39.0	37.0	39.0	32.0	39.0
14-15	37.909518788085606	40.0	38.0	41.0	32.0	41.0
16-17	37.92833171333513	40.0	38.0	41.0	32.0	41.0
18-19	37.94659890569541	40.0	38.0	41.0	32.0	41.0
20-21	38.05444317650549	40.0	38.0	41.0	32.0	41.0
22-23	38.06912606740237	40.0	38.0	41.0	32.0	41.0
24-25	38.092251923667774	40.0	38.0	41.0	32.0	41.0
26-27	38.1594041265154	40.0	38.0	41.0	32.0	41.0
28-29	38.13868963781729	40.0	38.0	41.0	32.0	41.0
30-31	38.10471713707728	40.0	38.0	41.0	32.0	41.0
32-33	38.075074422825736	40.0	38.0	41.0	32.0	41.0
34-35	38.02932039030178	40.0	38.0	41.0	32.0	41.0
36-37	37.95606295785156	40.0	38.0	41.0	32.0	41.0
38-39	37.90079696814404	40.0	38.0	41.0	32.0	41.0
40-41	37.8570995989876	40.0	38.0	41.0	31.5	41.0
42-43	37.706514137453716	40.0	37.5	41.0	31.0	41.0
44-45	37.59327877680131	40.0	37.0	41.0	31.0	41.0
46-47	37.486847556444395	40.0	37.0	41.0	31.0	41.0
48-49	37.39035736854727	40.0	37.0	41.0	30.0	41.0
50-51	37.268362484922484	40.0	36.5	41.0	30.0	41.0
52-53	37.15226583386833	40.0	36.0	41.0	30.0	41.0
54-55	37.037040344608656	40.0	36.0	41.0	30.0	41.0
56-57	36.86136758207654	39.0	35.0	41.0	30.0	41.0
58-59	36.68513888465859	39.0	35.0	41.0	30.0	41.0
60-61	36.4750676510657	39.0	35.0	41.0	30.0	41.0
62-63	36.25055763521859	38.5	35.0	40.5	29.5	41.0
64-65	35.9663824991698	38.0	35.0	40.0	29.0	41.0
66-67	35.73295579046105	37.0	35.0	40.0	29.0	41.0
68-69	35.477451074033674	37.0	34.5	40.0	29.0	41.0
70-71	35.185105912988504	36.0	34.0	39.0	29.0	41.0
72-73	34.838984697300845	36.0	34.0	39.0	28.0	41.0
74-75	34.504055811131174	35.0	34.0	38.5	28.0	40.0
76-77	34.21880088985067	35.0	34.0	37.5	28.0	39.5
78-79	33.89272955453352	35.0	34.0	37.0	28.0	39.0
80-81	33.62848867622736	35.0	34.0	36.5	27.5	39.0
82-83	33.3463780398623	35.0	33.0	36.0	27.0	38.0
84-85	33.11855000944794	35.0	33.0	36.0	27.0	37.0
86-87	32.89592558870695	35.0	33.0	35.5	26.5	37.0
88-89	32.69569248047796	35.0	33.0	35.0	26.0	36.5
90-91	32.53290927527087	35.0	33.0	35.0	26.0	36.0
92-93	32.36700529409695	35.0	33.0	35.0	25.5	36.0
94-95	32.23030785485723	35.0	33.0	35.0	25.0	36.0
96-97	32.12724832044087	35.0	33.0	35.0	25.0	35.0
98-99	32.01087030127827	35.0	33.0	35.0	25.0	35.0
100	30.872581794137904	34.0	31.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36860103414283074
1101	2	0.36280537170986094
1101	3	0.34535569773343155
1101	4	0.3484838033254505
1101	5	0.30714263174114365
1101	6	0.35868979562291514
1101	7	0.41851303558996733
1101	8	0.4073480961372624
1101	9	0.4766681457784756
1101	10-11	0.5315764626916462
1101	12-13	0.4965351332748682
1101	14-15	0.5642586286438203
1101	16-17	0.5361715623069401
1101	18-19	0.6221026471595366
1101	20-21	0.5814728886598814
1101	22-23	0.6157930699292606
1101	24-25	0.5985981297573773
1101	26-27	0.574166411218485
1101	28-29	0.595772361557259
1101	30-31	0.6615773044838775
1101	32-33	0.6820684196005473
1101	34-35	0.5603997843990456
1101	36-37	0.5920763711887318
1101	38-39	0.65074117475978
1101	40-41	0.6229217848696038
1101	42-43	0.6340559709281379
1101	44-45	0.5186840686076479
1101	46-47	0.44989156461372204
1101	48-49	0.5326877795151006
1101	50-51	0.45498971641841734
1101	52-53	0.4432917957234679
1101	54-55	0.42879743885195865
1101	56-57	0.43980762505196935
1101	58-59	0.4290362273346702
1101	60-61	0.40799971955349434
1101	62-63	0.41458097174700725
1101	64-65	0.43658908048260514
1101	66-67	0.42866178191333404
1101	68-69	0.3493454814076955
1101	70-71	0.35861049819012436
1101	72-73	0.40178910046101635
1101	74-75	0.39744392169257736
1101	76-77	0.3551946426893551
1101	78-79	0.4048325180645591
1101	80-81	0.3846798137523635
1101	82-83	0.3593173723363918
1101	84-85	0.27814309288556416
1101	86-87	0.3968827577096903
1101	88-89	0.3173200724578322
1101	90-91	0.3077245376689888
1101	92-93	0.3326529685183033
1101	94-95	0.365096956927637
1101	96-97	0.36389848972073224
1101	98-99	0.3945556884393753
1101	100	0.44253283410476385
1102	1	0.12513494371161826
1102	2	0.23663608850949913
1102	3	0.13966834248899573
1102	4	0.19919746629769008
1102	5	0.1542385498834875
1102	6	0.28411390678867576
1102	7	0.31507370650313504
1102	8	0.29477856363085664
1102	9	0.3457199203143517
1102	10-11	0.4457894806333087
1102	12-13	0.4592523759532341
1102	14-15	0.4980781047024223
1102	16-17	0.44255018447605465
1102	18-19	0.4199611293625125
1102	20-21	0.4246516851967783
1102	22-23	0.41432323014338834
1102	24-25	0.43099346332423494
1102	26-27	0.3904080106664978
1102	28-29	0.3837751589273424
1102	30-31	0.4025374684042262
1102	32-33	0.4268485233668642
1102	34-35	0.3866407308832791
1102	36-37	0.3887729948574261
1102	38-39	0.3468139434035251
1102	40-41	0.35293514316608565
1102	42-43	0.3409068120309229
1102	44-45	0.24867998402422842
1102	46-47	0.29850943759537785
1102	48-49	0.29924997197627334
1102	50-51	0.32814870435302623
1102	52-53	0.29009174430002815
1102	54-55	0.28328501891121505
1102	56-57	0.25549629029228527
1102	58-59	0.3261039114043385
1102	60-61	0.2854906950244285
1102	62-63	0.20588362636008384
1102	64-65	0.2738343483259129
1102	66-67	0.23866594989711842
1102	68-69	0.2591944230164671
1102	70-71	0.16391749694481206
1102	72-73	0.22116223081821573
1102	74-75	0.24244216825088927
1102	76-77	0.2553404610970773
1102	78-79	0.1541960309364967
1102	80-81	0.10324855782025111
1102	82-83	0.14790031073851395
1102	84-85	0.09229647957071307
1102	86-87	0.10779982398612731
1102	88-89	0.1755606178066671
1102	90-91	0.21003327318529585
1102	92-93	0.18797099239358772
1102	94-95	0.14876079978020584
1102	96-97	0.12313151968017877
1102	98-99	0.22252144587940137
1102	100	0.1807167323026242
1103	1	0.18409604217531594
1103	2	0.11910648640191468
1103	3	0.11911825157079292
1103	4	0.19970947179573528
1103	5	0.20490132210117906
1103	6	0.2900798589957745
1103	7	0.3614815068553554
1103	8	0.3051246255125051
1103	9	0.4540010651678088
1103	10-11	0.4431907950098335
1103	12-13	0.45552515919565195
1103	14-15	0.5368454829650986
1103	16-17	0.502253089563176
1103	18-19	0.43502706295642923
1103	20-21	0.41434886315304453
1103	22-23	0.45136416481114594
1103	24-25	0.4728071024863496
1103	26-27	0.4538205215838431
1103	28-29	0.4785465748172655
1103	30-31	0.578650178339366
1103	32-33	0.5293469842004512
1103	34-35	0.4882082465970896
1103	36-37	0.5329664000662504
1103	38-39	0.5045699103579793
1103	40-41	0.4235258967133291
1103	42-43	0.5469288901203555
1103	44-45	0.4327525467721003
1103	46-47	0.5464074373949259
1103	48-49	0.47953461037955236
1103	50-51	0.5048578949662641
1103	52-53	0.5078301003271122
1103	54-55	0.4136661594693294
1103	56-57	0.4693381928451501
1103	58-59	0.4691596220696894
1103	60-61	0.42275613322152594
1103	62-63	0.5068002536527274
1103	64-65	0.4404348666312501
1103	66-67	0.49121566855180987
1103	68-69	0.4564057398270194
1103	70-71	0.443400766563812
1103	72-73	0.3942913919367186
1103	74-75	0.48502496108624626
1103	76-77	0.3731339407585992
1103	78-79	0.3661466917042091
1103	80-81	0.35267169256532327
1103	82-83	0.3353895365736008
1103	84-85	0.30089562336466
1103	86-87	0.3261591441756906
1103	88-89	0.27051759273615517
1103	90-91	0.2379103689572375
1103	92-93	0.29946505765423836
1103	94-95	0.30974958892156224
1103	96-97	0.352966721489949
1103	98-99	0.3195545057422322
1103	100	0.42679665441364634
1104	1	0.1576778020711842
1104	2	0.13347936165549967
1104	3	0.20818512872150308
1104	4	0.20859425255306263
1104	5	0.22097621010843227
1104	6	0.27941344158652726
1104	7	0.3142625965257082
1104	8	0.325308937527403
1104	9	0.3979524876660605
1104	10-11	0.38461183925994646
1104	12-13	0.4208504055104427
1104	14-15	0.5081937578960307
1104	16-17	0.5248507673148737
1104	18-19	0.48896013733899935
1104	20-21	0.4323593735076088
1104	22-23	0.47239075919316065
1104	24-25	0.6008376919109182
1104	26-27	0.5892537794475672
1104	28-29	0.5605255402604925
1104	30-31	0.5890274571407232
1104	32-33	0.6012757519686929
1104	34-35	0.558913645097391
1104	36-37	0.5619792714425031
1104	38-39	0.543933888506416
1104	40-41	0.5775232933580483
1104	42-43	0.6195348072145705
1104	44-45	0.5391921122333372
1104	46-47	0.5287806449917483
1104	48-49	0.5458224750050746
1104	50-51	0.5801322512243559
1104	52-53	0.5303151968105126
1104	54-55	0.5779985996302415
1104	56-57	0.5829297845614931
1104	58-59	0.5501401038288947
1104	60-61	0.5029506232477701
1104	62-63	0.5259826200749842
1104	64-65	0.577490859541264
1104	66-67	0.5302193312511321
1104	68-69	0.46193447713046965
1104	70-71	0.44437806336708974
1104	72-73	0.4820934953924052
1104	74-75	0.49453046205904627
1104	76-77	0.44137923775573995
1104	78-79	0.4255820544083804
1104	80-81	0.2924508920335711
1104	82-83	0.3066457939734306
1104	84-85	0.3390938384045725
1104	86-87	0.3212684366308096
1104	88-89	0.32546334462809057
1104	90-91	0.3548428409677129
1104	92-93	0.38892084561926055
1104	94-95	0.28568883934911327
1104	96-97	0.2587222138079781
1104	98-99	0.3220661896838237
1104	100	0.4611500045026169
1105	1	0.01919963839655381
1105	2	-0.04958093948181741
1105	3	0.03606752606413721
1105	4	0.08097261285981006
1105	5	-0.02281822860434346
1105	6	0.1174604402005528
1105	7	0.11794950861415998
1105	8	0.11591062436662014
1105	9	0.12931838272520224
1105	10-11	0.25192418963482055
1105	12-13	0.21446228195217287
1105	14-15	0.2787388621339275
1105	16-17	0.2602102120532166
1105	18-19	0.2670213068705678
1105	20-21	0.1983432136613672
1105	22-23	0.22718860419236364
1105	24-25	0.27795986527431893
1105	26-27	0.18753572857642098
1105	28-29	0.19286297299984057
1105	30-31	0.1933930864951563
1105	32-33	0.24278717820185847
1105	34-35	0.2115768892534149
1105	36-37	0.18166128754277366
1105	38-39	0.16844114458537973
1105	40-41	0.1703128815636319
1105	42-43	0.19981626004214093
1105	44-45	0.14655702639262813
1105	46-47	0.15993583808246825
1105	48-49	0.16516384916384652
1105	50-51	0.20959467395654485
1105	52-53	0.2488128986909004
1105	54-55	0.16070523956153693
1105	56-57	0.2590109487045069
1105	58-59	0.18115439895473884
1105	60-61	0.14598153994495533
1105	62-63	0.1022994475342287
1105	64-65	0.19045605222504491
1105	66-67	0.20957971433401923
1105	68-69	0.18616870101341476
1105	70-71	0.22316852448842894
1105	72-73	0.15299853774423866
1105	74-75	0.10417767057712979
1105	76-77	0.0596690968524598
1105	78-79	0.11743394970211085
1105	80-81	0.075314029109812
1105	82-83	0.07725280288584457
1105	84-85	0.10870857722269989
1105	86-87	0.06959907169165547
1105	88-89	0.08822408565430351
1105	90-91	0.0889331401395026
1105	92-93	0.1812679650105693
1105	94-95	0.16450395595198586
1105	96-97	0.27462785858339345
1105	98-99	0.1851351109307089
1105	100	0.18910868223472832
1106	1	0.13763956280141088
1106	2	0.15115843376440097
1106	3	0.16259592000044876
1106	4	0.19185043133102653
1106	5	0.1833100304292543
1106	6	0.24263623816462143
1106	7	0.24491878357836327
1106	8	0.2626589175405627
1106	9	0.32923254326979645
1106	10-11	0.3118281610989442
1106	12-13	0.31504236106349026
1106	14-15	0.333959710092131
1106	16-17	0.38337321839193095
1106	18-19	0.41181178577774347
1106	20-21	0.40671929890125114
1106	22-23	0.40464704169164634
1106	24-25	0.4471800687993195
1106	26-27	0.3984713412523462
1106	28-29	0.3680603922759502
1106	30-31	0.39618597454927595
1106	32-33	0.4433679498409546
1106	34-35	0.41112925714184456
1106	36-37	0.40270226130990494
1106	38-39	0.34633130730050965
1106	40-41	0.3181060419553603
1106	42-43	0.3544827586327415
1106	44-45	0.272595934897474
1106	46-47	0.36062031894282853
1106	48-49	0.30756997192514035
1106	50-51	0.3450000843914225
1106	52-53	0.29226978152904337
1106	54-55	0.24465294700143403
1106	56-57	0.28895922750105996
1106	58-59	0.28748190630255266
1106	60-61	0.30563604420935775
1106	62-63	0.26813665286259436
1106	64-65	0.3195236863572717
1106	66-67	0.37107604508392456
1106	68-69	0.34028924979112674
1106	70-71	0.2808994669949385
1106	72-73	0.28413504550594837
1106	74-75	0.31478960086787566
1106	76-77	0.30349739975747525
1106	78-79	0.3319487413731892
1106	80-81	0.2339490305173797
1106	82-83	0.3169889464034341
1106	84-85	0.33266131164950963
1106	86-87	0.226881328424696
1106	88-89	0.265337278937281
1106	90-91	0.29642255614825075
1106	92-93	0.330641150895822
1106	94-95	0.30130606320780373
1106	96-97	0.3427843394410317
1106	98-99	0.3501405394137791
1106	100	0.33646376569877035
1107	1	0.052737270477702936
1107	2	0.10212480252842582
1107	3	0.10310695683620708
1107	4	0.10152620968477777
1107	5	0.09380928766232444
1107	6	0.06906938363367487
1107	7	0.12298385782410293
1107	8	0.07945305521664636
1107	9	0.15607672476266288
1107	10-11	0.22556546253369447
1107	12-13	0.23471952695523868
1107	14-15	0.2147673022241321
1107	16-17	0.19807063421897197
1107	18-19	0.16680863085719722
1107	20-21	0.17204138417309878
1107	22-23	0.18447689785958943
1107	24-25	0.18650675834990693
1107	26-27	0.18675223634136984
1107	28-29	0.1971632219450541
1107	30-31	0.24797072946190468
1107	32-33	0.23578283994046245
1107	34-35	0.19173608635304618
1107	36-37	0.23194848732312323
1107	38-39	0.24326320032759696
1107	40-41	0.2159528780190314
1107	42-43	0.2896194572521722
1107	44-45	0.19680112925770032
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2216	66-67	-0.6382945529555499
2216	68-69	-0.6650076975581811
2216	70-71	-0.6664901179763376
2216	72-73	-0.5432986357641099
2216	74-75	-0.5080367253880027
2216	76-77	-0.515001862813719
2216	78-79	-0.5275045558260487
2216	80-81	-0.5707829684221792
2216	82-83	-0.46157012347067905
2216	84-85	-0.4353186956814099
2216	86-87	-0.45552018147289175
2216	88-89	-0.45633523542933574
2216	90-91	-0.4835861709210363
2216	92-93	-0.4317290566638121
2216	94-95	-0.47272213935214324
2216	96-97	-0.4259631725717483
2216	98-99	-0.45571520503726504
2216	100	-0.45643967636803495
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1077.0
1	259.0
2	176.0
3	172.0
4	141.0
5	175.0
6	261.0
7	612.0
8	1634.0
9	2237.0
10	2405.0
11	3011.0
12	3942.0
13	5541.0
14	7505.0
15	8644.0
16	9414.0
17	10418.0
18	11380.0
19	12568.0
20	13938.0
21	15631.0
22	17620.0
23	20322.0
24	24520.0
25	29880.0
26	32521.0
27	31240.0
28	34126.0
29	42087.0
30	49520.0
31	55874.0
32	71655.0
33	94634.0
34	136509.0
35	207537.0
36	377107.0
37	771518.0
38	967659.0
39	305649.0
40	572.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47661879181083	20.205087464380973	13.1639820616547	24.1543116821535
2	14.814360002580266	21.032443280037448	40.929392471899675	23.22380424548261
3	17.885044395132958	26.388720363045117	30.736134696508465	24.99010054531346
4	11.83999545421453	18.089059513683818	40.36547996806112	29.705465064040524
5	12.897524357916126	38.77117897907621	35.75053397855681	12.580762684450859
6	29.210553031085563	39.26129137566531	18.80683942111583	12.721316172133305
7	25.774935057670444	33.41902534267678	23.38817681124505	17.417862788407724
8	24.56831935710432	36.140743531634676	21.3337149382028	17.957222173058202
9	25.774258175824237	15.88789554831512	21.019125998396056	37.318720277464585
10-11	23.755611180691858	26.893803078336344	29.572831112931524	19.777754628040277
12-13	24.438783821633564	24.85194747240582	28.77596643437432	21.9333022715863
14-15	22.025157843210952	26.244263811687883	26.239721028734657	25.490857316366505
16-17	21.83546251843048	28.92706058810612	27.015692024123727	22.221784869339675
18-19	21.682453584803362	28.759354215116023	28.14971360993537	21.408478590145247
20-21	22.803602717426205	28.062394151088398	27.54381401427013	21.59018911721527
22-23	22.80378248702058	27.88796245863728	27.14143220701255	22.16682284732959
24-25	22.45913142401218	27.92046290591127	27.48179787996574	22.13860779011081
26-27	22.393106571830142	28.09955911403776	27.173856913958065	22.333477400174033
28-29	22.15916304741893	28.403693482394548	27.27876469587221	22.158378774314315
30-31	22.549918816995216	28.06761067436647	27.19408687025458	22.188383638383726
32-33	22.41573221598811	28.256563204500633	26.930189305249353	22.397515274261902
34-35	22.578231064999702	28.26924562484073	27.236444916160973	21.916078393998596
36-37	22.600055376877236	28.005531176279163	27.17071957235199	22.223693874491605
38-39	22.413911494104884	28.10582348841029	27.28352638409777	22.196738633387053
40-41	22.52427193086434	27.74880995137697	27.557540906048494	22.169377211710195
42-43	22.357434571121253	27.87214589436783	27.279155520379426	22.49126401413149
44-45	22.318258065518627	28.197027961549814	27.33841769992963	22.146296273001926
46-47	22.35742346262664	27.990075146713	27.122013039873295	22.530488350787063
48-49	22.3636080559734	27.86544587448371	27.305264940082203	22.465681129460684
50-51	22.330750257244546	27.93315386028125	27.148197275523888	22.587898606950315
52-53	22.25300108734888	27.97838799751539	27.12085201266102	22.647758902474706
54-55	22.152605794139564	27.90033659669589	27.233802625143454	22.71325498402109
56-57	22.4820353104531	27.77744889420628	27.136190062079073	22.604325733261543
58-59	22.23587639961691	27.760282900478806	27.34214242380873	22.661698276095557
60-61	22.208961320643716	27.88412399238978	27.231202191008784	22.67571249595772
62-63	22.26016535232276	27.698637910204283	27.15781880332469	22.883377934148267
64-65	21.886341398508723	27.7214509234593	27.229228400456627	23.162979277575346
66-67	22.165258433598435	27.616716686359283	27.19093876136174	23.027086118680547
68-69	22.148886195214434	27.46665837843758	27.30284745070354	23.081607975644445
70-71	22.05789521280556	27.307260294308534	27.250617527524728	23.38422696536118
72-73	22.1079929692324	27.300036645715263	27.410551005921924	23.181419379130414
74-75	22.011175598812788	27.594416315255316	27.260581081999142	23.13382700393276
76-77	22.002473742937052	27.409838404526088	27.21904613336692	23.36864171916994
78-79	22.063690590725418	27.433963381240655	27.246621571616764	23.255724456417166
80-81	21.909075139512332	27.370185152075248	27.369474713722948	23.351264994689476
82-83	22.147991637218077	27.07004384909989	27.694558641232952	23.087405872449075
84-85	22.237120088378767	27.233631530758956	27.321282095988174	23.207966284874104
86-87	21.829106066768375	27.32903709729077	27.53008460453257	23.311772231408288
88-89	22.18277632176409	26.96299883433393	27.378885282237412	23.475339561664562
90-91	22.05444612575748	26.97375198027891	27.443646412764718	23.528155481198894
92-93	22.19235033751645	26.885983788081226	27.48345272549787	23.438213148904456
94-95	21.787464686741483	26.774779366593837	27.70507070939377	23.73268523727091
96-97	21.832908868679482	26.75826263156641	27.596413215711564	23.81241528404255
98-99	21.819109991544043	26.91056473751525	27.403520597355048	23.866804673585662
100	22.070919563842814	26.681381917375823	27.14696960399553	24.100728914785833
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2082.0
1	1399.0
2	751.0
3	766.0
4	690.5
5	584.0
6	520.0
7	512.0
8	553.0
9	583.5
10	526.0
11	492.0
12	500.0
13	496.0
14	540.0
15	617.5
16	714.5
17	833.5
18	1070.5
19	1456.0
20	2006.5
21	2798.0
22	3987.0
23	5862.0
24	8428.5
25	11900.0
26	16624.0
27	21566.5
28	27003.5
29	33939.0
30	40692.5
31	48047.0
32	57394.5
33	66000.0
34	74243.5
35	82522.0
36	90908.5
37	100545.5
38	105313.0
39	106459.0
40	108146.0
41	109559.0
42	111872.0
43	116456.5
44	121721.0
45	129239.5
46	135553.0
47	139806.0
48	141546.5
49	138206.5
50	132736.0
51	129031.5
52	125275.5
53	119137.5
54	112184.5
55	101594.0
56	90859.0
57	78465.5
58	66822.5
59	58160.5
60	47287.0
61	36969.5
62	29338.0
63	22467.5
64	15921.0
65	10939.5
66	8009.5
67	5813.0
68	4261.5
69	3039.5
70	2181.0
71	1701.5
72	1425.5
73	1297.0
74	1048.0
75	757.0
76	528.5
77	361.0
78	247.5
79	174.0
80	137.5
81	93.0
82	67.0
83	59.5
84	60.0
85	51.5
86	40.5
87	29.0
88	21.5
89	18.0
90	13.5
91	9.5
92	9.5
93	10.0
94	8.0
95	7.0
96	5.5
97	6.5
98	5.5
99	3.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09551434474645969
2	0.06484921301207
3	0.08034441940437491
4	0.08093583949568425
5	0.08309452282896337
6	0.08823987762335471
7	0.08646561734942666
8	0.0880624515959619
9	0.0745485025095433
10-11	0.08052184543176771
12-13	0.07098519645940447
14-15	0.07998956734958931
16-17	0.08211867967830296
18-19	0.08081755547742239
20-21	0.07286295524931166
22-23	0.07663325833140876
24-25	0.07951643127654183
26-27	0.07311430878811813
28-29	0.08179339862808281
30-31	0.08590376826268278
32-33	0.08776674155030723
34-35	0.08912700776031873
36-37	0.08943750330825613
38-39	0.09007327990641369
40-41	0.08701268093388781
42-43	0.09276424132187122
44-45	0.08970364234934534
46-47	0.08577069874213818
48-49	0.09548477374189422
50-51	0.09156661563696979
52-53	0.09730339052267047
54-55	0.08926007728086333
56-57	0.09634233287429278
58-59	0.10055620102487188
60-61	0.0987227987418129
62-63	0.10023091997465174
64-65	0.10179818321662151
66-67	0.10413429257729342
68-69	0.10071884154998195
70-71	0.1035428724859841
72-73	0.10045270250889274
74-75	0.10431171860468623
76-77	0.1008223400659611
78-79	0.10766802762286677
80-81	0.10335066095630853
82-83	0.10729839006579844
84-85	0.10361679999739774
86-87	0.10009785045410713
88-89	0.0995064303627978
90-91	0.09992042442671432
92-93	0.09430193355927552
94-95	0.09402100901590359
96-97	0.09230589075110647
98-99	0.1064408309333999
100	0.2460307579846887
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3381691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.144818885392056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69787777212997	41.107152986420814
2	15.214477296575293	17.6928605369056
3	6.1255101858000796	10.685000410019097
4	3.0048101455745955	6.9885656679769355
5	1.618156406688362	4.704370559756581
6	1.0011027872525073	3.4925364150274927
7	0.6384409913179757	2.5985425066435703
8	0.4077538819113776	1.8967020490842073
9	0.2897955071545436	1.516509654957115
>10	0.9878542241446322	8.578869202693813
>50	0.011427928904007786	0.43436548119136253
>100	0.0027108900619569522	0.21300088008475265
>500	0.0	0.0
>1k	8.19824848122205E-5	0.09152364923867455
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	8.871301369640218E-5
6	0.0	0.0	0.0	0.0	8.871301369640218E-5
7	0.0	0.0	0.0	0.0	1.1828401826186958E-4
8	2.9571004565467394E-5	0.0	0.0	0.0	1.1828401826186958E-4
9	2.9571004565467394E-5	0.0	0.0	0.0	1.1828401826186958E-4
10-11	2.9571004565467394E-5	0.0	0.0	0.0	1.1828401826186958E-4
12-13	2.9571004565467394E-5	0.0	0.0	0.0	1.774260273928044E-4
14-15	2.9571004565467394E-5	0.0	0.0	1.4785502282733697E-5	2.2178253424100545E-4
16-17	2.9571004565467394E-5	0.0	0.0	2.9571004565467394E-5	2.3656803652373915E-4
18-19	2.9571004565467394E-5	0.0	0.0	5.914200913093479E-5	2.3656803652373915E-4
20-21	2.9571004565467394E-5	0.0	0.0	1.626405251100707E-4	2.3656803652373915E-4
22-23	2.9571004565467394E-5	0.0	0.0	3.6963755706834244E-4	2.3656803652373915E-4
24-25	2.9571004565467394E-5	0.0	0.0	0.0010497706620740926	2.5135353880647286E-4
26-27	2.9571004565467394E-5	0.0	0.0	0.0019812573058863156	2.6613904108920656E-4
28-29	2.9571004565467394E-5	0.0	0.0	0.006062055935920817	2.6613904108920656E-4
30-31	2.9571004565467394E-5	0.0	0.0	0.016825901597750947	2.6613904108920656E-4
32-33	2.9571004565467394E-5	0.0	0.0	0.03992085616338098	2.6613904108920656E-4
34-35	2.9571004565467394E-5	0.0	0.0	0.07616012225836127	2.6613904108920656E-4
36-37	2.9571004565467394E-5	0.0	0.0	0.13525777488244786	2.6613904108920656E-4
38-39	2.9571004565467394E-5	0.0	0.0	0.22339415398982343	2.6613904108920656E-4
40-41	2.9571004565467394E-5	0.0	0.0	0.3244974185991565	2.6613904108920656E-4
42-43	2.9571004565467394E-5	0.0	0.0	0.4258816077518614	2.6613904108920656E-4
44-45	2.9571004565467394E-5	0.0	0.0	0.5525342203057583	2.6613904108920656E-4
46-47	2.9571004565467394E-5	0.0	0.0	0.7101624601419823	2.6613904108920656E-4
48-49	2.9571004565467394E-5	0.0	0.0	0.8849270971238945	2.6613904108920656E-4
50-51	2.9571004565467394E-5	0.0	0.0	1.069184026571322	2.8092454337194027E-4
52-53	2.9571004565467394E-5	0.0	0.0	1.247068404534891	2.9571004565467397E-4
54-55	2.9571004565467394E-5	0.0	0.0	1.4478111690275663	2.9571004565467397E-4
56-57	2.9571004565467394E-5	0.0	0.0	1.685798613770448	2.9571004565467397E-4
58-59	2.9571004565467394E-5	0.0	0.0	1.958310206343513	3.252810502201414E-4
60-61	2.9571004565467394E-5	0.0	0.0	2.248120836587376	3.8442305935107614E-4
62-63	2.9571004565467394E-5	0.0	0.0	2.535462287950023	4.1399406391654355E-4
64-65	2.9571004565467394E-5	0.0	0.0	2.8340702920521124	4.1399406391654355E-4
66-67	2.9571004565467394E-5	0.0	0.0	3.1845014816551838	4.1399406391654355E-4
68-69	2.9571004565467394E-5	0.0	0.0	3.575193593974139	4.1399406391654355E-4
70-71	2.9571004565467394E-5	0.0	0.0	4.003751377639175	4.2877956619927725E-4
72-73	2.9571004565467394E-5	0.0	0.0	4.434512201144338	4.731360730474783E-4
74-75	2.9571004565467394E-5	0.0	0.0	4.864592891544497	4.731360730474783E-4
76-77	2.9571004565467394E-5	0.0	0.0	5.334136087537271	5.027070776129457E-4
78-79	4.435650684820109E-5	0.0	0.0	5.861963733528581	5.027070776129457E-4
80-81	5.914200913093479E-5	0.0	0.0	6.434325312395485	5.027070776129457E-4
82-83	5.914200913093479E-5	0.0	0.0	7.02967834731204	5.322780821784131E-4
84-85	5.914200913093479E-5	0.0	0.0	7.615154666703729	5.322780821784131E-4
86-87	5.914200913093479E-5	0.0	0.0	8.22168554134603	5.470635844611468E-4
88	5.914200913093479E-5	0.0	0.0	8.70061753128834	5.618490867438805E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3820	0.0	35.456596	1
GTATCAA	5905	0.0	32.494373	1
CAACGCA	6275	0.0	28.543806	5
ATCAACG	6370	0.0	28.045145	3
GTACATG	9715	0.0	26.140799	1
TATCAAC	7285	0.0	26.006927	2
AACGCAG	7100	0.0	25.880028	6
TACATGG	9740	0.0	25.340425	2
TCAACGC	7180	0.0	25.012232	4
GTACTAG	1270	0.0	24.8107	1
ACATGGG	9840	0.0	23.8407	3
ACGCAGA	7635	0.0	23.634653	7
CGCAGAG	7770	0.0	23.10203	8
CTGTGCG	1635	0.0	20.978806	9
CATGGGG	7275	0.0	20.872913	4
CTAGTAC	1800	0.0	19.849731	3
GCATCGT	1405	0.0	19.063662	8
CTCGTCG	900	0.0	18.79471	9
GTGATCG	755	0.0	18.671623	8
TAGTACT	2075	0.0	18.57844	4
>>END_MODULE