FastQCFastQC Report
Mon 16 Jan 2017
ERR1146518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381691
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115010.3400961235074405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104010.30756801848542636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58480.17293123469885333No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68600.058.9631
GTATCAA124700.041.036251
ATCAACG152700.032.9532743
CAACGCA154950.032.485415
TATCAAC159050.032.0248572
TCAACGC162400.031.0424614
AACGCAG165000.030.8471976
ACGCAGA179800.028.0728077
CGCAGAG180700.027.7769348
GCAGAGT202650.024.6059449
TACATGG93550.024.0815332
GTACATG97800.024.0456451
GAGTACT141700.023.18381712-13
ACATGGG94850.022.8073253
TACGGGC7500.022.572974
CAGAGTA195450.022.41049610-11
CTAACGC5250.022.3941463
GTACTAG14300.022.0365721
TAACGCC4750.021.7809334
AGAGTAC184800.021.74379510-11
Produced by FastQC (version 0.11.5)