##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146518_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3381691
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34776211073099	33.0	31.0	34.0	30.0	34.0
2	32.40417087190994	34.0	31.0	34.0	30.0	34.0
3	32.511480498957475	34.0	31.0	34.0	30.0	34.0
4	36.02566171776191	37.0	35.0	37.0	35.0	37.0
5	36.07051176467631	37.0	35.0	37.0	35.0	37.0
6	36.13721478396459	37.0	36.0	37.0	35.0	37.0
7	36.10146757938558	37.0	36.0	37.0	35.0	37.0
8	36.10261049871203	37.0	36.0	37.0	35.0	37.0
9	37.88107636090938	39.0	38.0	39.0	35.0	39.0
10-11	37.833959105074946	39.0	38.0	39.0	35.0	39.0
12-13	37.80863671459042	39.0	38.0	39.0	35.0	39.0
14-15	39.15082720449621	40.0	39.0	41.0	36.0	41.0
16-17	39.094811885533005	40.0	39.0	41.0	36.0	41.0
18-19	39.20937912423104	40.0	39.0	41.0	36.0	41.0
20-21	39.27261863960959	40.5	39.0	41.0	36.0	41.0
22-23	39.2370024345808	40.0	39.0	41.0	36.0	41.0
24-25	39.23080656984922	41.0	39.0	41.0	36.0	41.0
26-27	39.153424573682216	40.5	39.0	41.0	35.5	41.0
28-29	39.09156838989725	40.0	39.0	41.0	35.0	41.0
30-31	38.942028263374745	40.0	38.5	41.0	35.0	41.0
32-33	38.82754885056026	40.0	38.0	41.0	35.0	41.0
34-35	38.75454114524361	40.0	38.0	41.0	35.0	41.0
36-37	38.67931561458454	40.0	38.0	41.0	35.0	41.0
38-39	38.5965716560147	40.0	38.0	41.0	35.0	41.0
40-41	38.495460407234134	40.0	38.0	41.0	34.5	41.0
42-43	38.386957590152385	40.0	38.0	41.0	34.0	41.0
44-45	38.239953177271374	40.0	38.0	41.0	34.0	41.0
46-47	37.995146363165645	40.0	38.0	41.0	33.0	41.0
48-49	37.713970761964944	40.0	37.5	41.0	33.0	41.0
50-51	37.607302086441365	40.0	37.0	41.0	33.0	41.0
52-53	37.50739822177721	40.0	37.0	41.0	33.0	41.0
54-55	37.35209381933477	40.0	36.5	41.0	32.0	41.0
56-57	37.20981233944793	40.0	36.0	41.0	32.0	41.0
58-59	37.042988700032026	39.0	36.0	41.0	32.0	41.0
60-61	36.85367779019431	39.0	35.0	41.0	31.5	41.0
62-63	36.62872450498878	39.0	35.0	41.0	31.0	41.0
64-65	36.363418183388134	38.5	35.0	41.0	31.0	41.0
66-67	36.10568322179643	37.5	35.0	40.0	31.0	41.0
68-69	35.81032699321138	37.0	35.0	40.0	31.0	41.0
70-71	35.505843378357156	36.5	35.0	39.5	30.0	41.0
72-73	35.180855524647285	36.0	35.0	39.0	30.0	41.0
74-75	34.85961890072156	36.0	35.0	39.0	30.0	40.5
76-77	34.55703034369492	35.0	34.5	37.5	30.0	39.5
78-79	34.26285621601737	35.0	34.0	37.0	29.5	39.0
80-81	33.99033146434728	35.0	34.0	37.0	29.0	39.0
82-83	33.72608733323062	35.0	34.0	36.0	29.0	38.0
84-85	33.50477394297705	35.0	34.0	36.0	29.0	37.0
86-87	33.310496287212516	35.0	34.0	35.5	29.0	37.0
88-89	33.1104883621833	35.0	34.0	35.0	29.0	36.5
90-91	33.01612625162973	35.0	34.0	35.0	29.0	36.0
92-93	32.91218003064148	35.0	34.0	35.0	29.0	36.0
94-95	32.83440828863429	35.0	34.0	35.0	29.0	36.0
96-97	32.77482404513009	35.0	34.0	35.0	29.0	35.5
98-99	32.70438014590925	35.0	34.0	35.0	29.0	35.0
100	31.9762769572974	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3217362522222871
1101	2	0.3240973671211478
1101	3	0.31981066229155175
1101	4	0.2950275138274847
1101	5	0.25006044765773083
1101	6	0.29084930726345704
1101	7	0.30233427424863635
1101	8	0.2848153940611198
1101	9	0.32309202134023707
1101	10-11	0.3362529470879281
1101	12-13	0.34056939758266225
1101	14-15	0.4900419117194019
1101	16-17	0.5353472933127321
1101	18-19	0.45135272839323903
1101	20-21	0.4389812039551728
1101	22-23	0.3989011409750489
1101	24-25	0.4320922561506393
1101	26-27	0.3900590666529311
1101	28-29	0.39295659409523154
1101	30-31	0.4498815740155422
1101	32-33	0.39723575437537306
1101	34-35	0.42444515956324125
1101	36-37	0.37511104602236855
1101	38-39	0.4051909378744796
1101	40-41	0.393993773152836
1101	42-43	0.40794720492996106
1101	44-45	0.3669109794649259
1101	46-47	0.37034556789529915
1101	48-49	0.46845230161900275
1101	50-51	0.4423638215358352
1101	52-53	0.4538440456422492
1101	54-55	0.3954221059319707
1101	56-57	0.3310756436064679
1101	58-59	0.35791921451791353
1101	60-61	0.32949200555562186
1101	62-63	0.3597037578057325
1101	64-65	0.28582171941490486
1101	66-67	0.27964920754114075
1101	68-69	0.26467796076524763
1101	70-71	0.3052361729061772
1101	72-73	0.3149831379936643
1101	74-75	0.2621729641395376
1101	76-77	0.25881549875779086
1101	78-79	0.22563011238539588
1101	80-81	0.2415521923508166
1101	82-83	0.2664943358144143
1101	84-85	0.27122849563638596
1101	86-87	0.31056632397984174
1101	88-89	0.39429293548990785
1101	90-91	0.33021277287109996
1101	92-93	0.2720694781786861
1101	94-95	0.2584532576724996
1101	96-97	0.2779086145083767
1101	98-99	0.31574795743999573
1101	100	0.35011150739448027
1102	1	0.2907910576492512
1102	2	0.23154951463970264
1102	3	0.2536738726846153
1102	4	0.1751162070577763
1102	5	0.14459601546975165
1102	6	0.165090313145015
1102	7	0.21094355089088168
1102	8	0.22852177923326877
1102	9	0.2310193474881217
1102	10-11	0.2595505016673556
1102	12-13	0.3009994362763635
1102	14-15	0.3943327788105577
1102	16-17	0.4361923016792204
1102	18-19	0.3574211419109332
1102	20-21	0.3622931501818769
1102	22-23	0.40299121661713144
1102	24-25	0.3969090520064924
1102	26-27	0.41568403029241097
1102	28-29	0.3882219964124616
1102	30-31	0.4453930582449388
1102	32-33	0.43292978571681573
1102	34-35	0.4676322554529051
1102	36-37	0.45887323010282444
1102	38-39	0.5009742665902763
1102	40-41	0.4782632755130649
1102	42-43	0.45689416352229273
1102	44-45	0.4636341386867855
1102	46-47	0.4875155455562066
1102	48-49	0.5362436221233224
1102	50-51	0.5112651296196091
1102	52-53	0.49417712023105764
1102	54-55	0.48963654167972237
1102	56-57	0.4549174465513559
1102	58-59	0.41230326625380087
1102	60-61	0.3214357851703724
1102	62-63	0.4001925469667782
1102	64-65	0.4149870079414981
1102	66-67	0.3979584137609393
1102	68-69	0.3922558963663363
1102	70-71	0.32027605455088803
1102	72-73	0.3601186493375437
1102	74-75	0.3105395274304428
1102	76-77	0.32673522530225085
1102	78-79	0.3833925194092487
1102	80-81	0.34746591854459297
1102	82-83	0.3348392007729615
1102	84-85	0.33492058680070613
1102	86-87	0.36821407585197363
1102	88-89	0.34929554327673884
1102	90-91	0.29382107718721073
1102	92-93	0.3059655198349276
1102	94-95	0.30954482597759636
1102	96-97	0.32739594640071346
1102	98-99	0.32237970986867737
1102	100	0.3587402915840485
1103	1	0.18189138266392746
1103	2	0.18785036082328332
1103	3	0.21062159829777727
1103	4	0.14442079019423915
1103	5	0.13347450007093187
1103	6	0.1794035585668894
1103	7	0.14997824298600904
1103	8	0.13532503797072337
1103	9	0.1329428043842995
1103	10-11	0.22934446813501097
1103	12-13	0.2221952611979745
1103	14-15	0.3231906457406737
1103	16-17	0.4250510276789541
1103	18-19	0.31019092556856265
1103	20-21	0.2829383114722006
1103	22-23	0.2982634700999469
1103	24-25	0.3123191861645296
1103	26-27	0.3116285057588115
1103	28-29	0.32818519959674575
1103	30-31	0.3903564761966294
1103	32-33	0.2842620595309455
1103	34-35	0.32450710332599186
1103	36-37	0.2771974716159349
1103	38-39	0.3279116833679083
1103	40-41	0.3139096538186976
1103	42-43	0.36664889914361254
1103	44-45	0.391835533243146
1103	46-47	0.47575345057174445
1103	48-49	0.42631048637600344
1103	50-51	0.41124193473966386
1103	52-53	0.418038924563632
1103	54-55	0.45886395172797734
1103	56-57	0.4337485391447444
1103	58-59	0.41453226558085987
1103	60-61	0.346693004934707
1103	62-63	0.4318263542201919
1103	64-65	0.42317651870133943
1103	66-67	0.32478773094376834
1103	68-69	0.3172672790389015
1103	70-71	0.40456395741875895
1103	72-73	0.3976120273424897
1103	74-75	0.39972069089707674
1103	76-77	0.3758974892370759
1103	78-79	0.38417613408788753
1103	80-81	0.38692355925304156
1103	82-83	0.3336745887144872
1103	84-85	0.34647765050071655
1103	86-87	0.3609397606020508
1103	88-89	0.4149231652288279
1103	90-91	0.30898823781891593
1103	92-93	0.37563205764114826
1103	94-95	0.39269302980618903
1103	96-97	0.3645391103541513
1103	98-99	0.30718434311753384
1103	100	0.39592712491022297
1104	1	0.25979568166204814
1104	2	0.23967333266411828
1104	3	0.2325818694344619
1104	4	0.20629949876384757
1104	5	0.21718192724968333
1104	6	0.23049412902019384
1104	7	0.17457164181865892
1104	8	0.15801037124866468
1104	9	0.1977108218877106
1104	10-11	0.22091845950793498
1104	12-13	0.2459249830682424
1104	14-15	0.3809341737025065
1104	16-17	0.40695161551104064
1104	18-19	0.35966748498530166
1104	20-21	0.3377170608923237
1104	22-23	0.3775602179829889
1104	24-25	0.35865877170483884
1104	26-27	0.3483534709666358
1104	28-29	0.33805821678858194
1104	30-31	0.3605754765358995
1104	32-33	0.3343278357947099
1104	34-35	0.3331003913775561
1104	36-37	0.2815220186251892
1104	38-39	0.2759649136748408
1104	40-41	0.2896544834581718
1104	42-43	0.28593623063521534
1104	44-45	0.20951335417938566
1104	46-47	0.2730275437247798
1104	48-49	0.36108245045523546
1104	50-51	0.266056480294921
1104	52-53	0.22731829366540524
1104	54-55	0.2713617880350867
1104	56-57	0.27598417049301815
1104	58-59	0.22964875880590796
1104	60-61	0.20148760989631143
1104	62-63	0.24622512455025003
1104	64-65	0.24722542854632223
1104	66-67	0.24246444406924184
1104	68-69	0.24110556636346558
1104	70-71	0.21055506723742212
1104	72-73	0.21712057479487612
1104	74-75	0.17778264521373188
1104	76-77	0.1856142358738353
1104	78-79	0.1760846843097923
1104	80-81	0.16367012677064707
1104	82-83	0.11372063209912397
1104	84-85	0.16858373722845243
1104	86-87	0.21879850088274821
1104	88-89	0.24998994544609587
1104	90-91	0.10677670135464012
1104	92-93	0.19549572767971313
1104	94-95	0.18863456509341603
1104	96-97	0.22911395335658824
1104	98-99	0.2230867588373755
1104	100	0.32345400663065504
1105	1	0.12332001119652602
1105	2	0.1499436040845339
1105	3	0.13902761553939058
1105	4	0.09538773267335188
1105	5	0.042434007605805846
1105	6	0.09569035021523575
1105	7	0.15873229023388546
1105	8	0.11337969413786197
1105	9	0.12814233536990116
1105	10-11	0.147117182689108
1105	12-13	0.1658227944753463
1105	14-15	0.2604227945461517
1105	16-17	0.2969145604766581
1105	18-19	0.20529092423613093
1105	20-21	0.14647324105340687
1105	22-23	0.21743510005823907
1105	24-25	0.23675483658891494
1105	26-27	0.23328644502678486
1105	28-29	0.25933601704088716
1105	30-31	0.25563395417827195
1105	32-33	0.19978683601626557
1105	34-35	0.18880644643933664
1105	36-37	0.19017944387479702
1105	38-39	0.1243263880353993
1105	40-41	0.1490804043749847
1105	42-43	0.21161099158742047
1105	44-45	0.2902867197696466
1105	46-47	0.28172205858496113
1105	48-49	0.3536521607041223
1105	50-51	0.31511650003525915
1105	52-53	0.19379860797890558
1105	54-55	0.23601215761463834
1105	56-57	0.17228300237641037
1105	58-59	0.23810210475689075
1105	60-61	0.18417311845750817
1105	62-63	0.2677872341551648
1105	64-65	0.2847425204867946
1105	66-67	0.20143454444392006
1105	68-69	0.14057756138024757
1105	70-71	0.19051364496455392
1105	72-73	0.20659458586936807
1105	74-75	0.1971116650346616
1105	76-77	0.15368219563672625
1105	78-79	0.23817061783893223
1105	80-81	0.16540246026701055
1105	82-83	0.1614667567069219
1105	84-85	0.13560091334864666
1105	86-87	0.08842067358122563
1105	88-89	0.18267562688823347
1105	90-91	0.11945725222245329
1105	92-93	0.18267939878251127
1105	94-95	0.16600730942937503
1105	96-97	0.16093649346696282
1105	98-99	0.14957858170196658
1105	100	0.16236354814242304
1106	1	0.20734895256967434
1106	2	0.13574678463094614
1106	3	0.17158122207535342
1106	4	0.1495592523452629
1106	5	0.1375775099830463
1106	6	0.1564438153395784
1106	7	0.16052742420232136
1106	8	0.17191545588995183
1106	9	0.19511873614753483
1106	10-11	0.15935019729821676
1106	12-13	0.18374819623280558
1106	14-15	0.2725087425967061
1106	16-17	0.3444866940425797
1106	18-19	0.22431198566284394
1106	20-21	0.19398073161230656
1106	22-23	0.22398951434501413
1106	24-25	0.21035304352827922
1106	26-27	0.1816927431718014
1106	28-29	0.20972899893888552
1106	30-31	0.23263442688158875
1106	32-33	0.22714329773805275
1106	34-35	0.1921068936253434
1106	36-37	0.1878066652638637
1106	38-39	0.23436165936736586
1106	40-41	0.1965579208021282
1106	42-43	0.22098824256823946
1106	44-45	0.2465491082631175
1106	46-47	0.2199418891646303
1106	48-49	0.32051708575264826
1106	50-51	0.19331887806518466
1106	52-53	0.19434865772619503
1106	54-55	0.24758336208869025
1106	56-57	0.21419826739659698
1106	58-59	0.2739222756013646
1106	60-61	0.2406101599074617
1106	62-63	0.18140684232720616
1106	64-65	0.2651604903165392
1106	66-67	0.2199093374099732
1106	68-69	0.14829894870286608
1106	70-71	0.1458438613280748
1106	72-73	0.20190602410851
1106	74-75	0.2183114273489295
1106	76-77	0.1784127024880391
1106	78-79	0.1107506474961113
1106	80-81	0.20202645192745905
1106	82-83	0.13557824144313457
1106	84-85	0.1712980224317988
1106	86-87	0.1316242739439275
1106	88-89	0.20314834125127845
1106	90-91	0.15598248432335282
1106	92-93	0.19530018646105418
1106	94-95	0.15719119713757124
1106	96-97	0.14217825270505102
1106	98-99	0.16836868723895293
1106	100	0.18205648897310311
1107	1	0.07359101331601892
1107	2	0.05401834478879408
1107	3	0.07155769984954219
1107	4	0.004372132074259127
1107	5	0.026524708695767174
1107	6	0.07088662276787971
1107	7	0.04802309417536321
1107	8	0.030387625422974907
1107	9	0.04076188693922944
1107	10-11	0.04629400739555223
1107	12-13	0.10309644487607983
1107	14-15	0.1488860408672963
1107	16-17	0.19714786490116154
1107	18-19	0.1504775798816098
1107	20-21	0.10213293134638946
1107	22-23	0.11900683003137402
1107	24-25	0.07718802353799958
1107	26-27	0.12254758957431733
1107	28-29	0.17146697549206635
1107	30-31	0.1770928525533222
1107	32-33	0.1467738774033478
1107	34-35	0.1017401454669482
1107	36-37	0.061136384245614295
1107	38-39	0.030912855700847786
1107	40-41	0.07284837736241911
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2216	42-43	-0.5882007320084028
2216	44-45	-0.5779984603429611
2216	46-47	-0.5032400859010124
2216	48-49	-0.41039877404159597
2216	50-51	-0.49127288371563793
2216	52-53	-0.5442074241888406
2216	54-55	-0.47263854531826155
2216	56-57	-0.4569051607529957
2216	58-59	-0.4978669838159746
2216	60-61	-0.5322878229130694
2216	62-63	-0.5650257789423705
2216	64-65	-0.44492522972745263
2216	66-67	-0.4653642761254062
2216	68-69	-0.40889719836854255
2216	70-71	-0.41926133870214244
2216	72-73	-0.3602807237769525
2216	74-75	-0.4051045158662916
2216	76-77	-0.42272163922924477
2216	78-79	-0.4159319885106356
2216	80-81	-0.388076391103894
2216	82-83	-0.4252018894078162
2216	84-85	-0.3743117735404269
2216	86-87	-0.3481222833022741
2216	88-89	-0.28783688997425827
2216	90-91	-0.3624894376941441
2216	92-93	-0.3363910958849985
2216	94-95	-0.3056688482903027
2216	96-97	-0.3074172236046877
2216	98-99	-0.34052045935270314
2216	100	-0.4024616597282282
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	21.0
8	105.0
9	224.0
10	285.0
11	193.0
12	219.0
13	316.0
14	502.0
15	960.0
16	1789.0
17	3047.0
18	5174.0
19	7923.0
20	11168.0
21	16275.0
22	22752.0
23	25975.0
24	24295.0
25	20885.0
26	19496.0
27	21895.0
28	27035.0
29	33047.0
30	41061.0
31	51910.0
32	66359.0
33	86111.0
34	122725.0
35	188823.0
36	335608.0
37	761328.0
38	1091652.0
39	391493.0
40	1039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45976615844661	19.91595117822715	13.529488651250324	24.09479401207592
2	15.067187527723727	21.19710312930642	40.41575239976579	23.319956943204065
3	17.688139991791072	26.576196948029445	31.305201787038644	24.43046127314084
4	11.894358106798716	18.241824501981835	39.80420403041823	30.05961336080122
5	12.351909127658525	38.51830910778641	35.825874143865235	13.303907620689829
6	28.80750852717488	39.54663286749063	18.282073681178588	13.363784924155903
7	25.04537671992406	34.01686730599198	23.299030957893834	17.63872501619013
8	24.836427552915975	35.60201698444738	21.07004739061804	18.4915080720186
9	25.9429829295361	14.865737210015467	21.15466561236116	38.036614248087275
10-11	23.83557309447805	26.490980088328747	29.385069869015002	20.288376948178204
12-13	25.092121071972574	24.523825506233422	29.012245648700606	21.3718077730934
14-15	22.217382433043834	25.563017307914087	27.336952825362467	24.88264743367961
16-17	21.172490147657246	28.366910795600887	28.045251432496777	22.41534762424509
18-19	20.950200580420535	27.24228387851504	30.641934230440082	21.165581310624347
20-21	22.024659603815792	26.49549359202492	30.43722736405481	21.042619440104477
22-23	21.88452463575176	26.428686121824853	30.093391146618657	21.593398095804734
24-25	21.64142140721905	26.400209244428307	30.449559111107433	21.508810237245214
26-27	21.580479072036162	26.683987428155238	30.10439918460731	21.63113431520129
28-29	21.369238255245413	26.95304315992253	30.214193167588814	21.46352541724324
30-31	21.833785375824142	26.563523896941412	30.09646365956883	21.506227067665616
32-33	21.73471406431169	26.716576667207814	29.832137036928607	21.716572231551883
34-35	21.894511324219547	26.753723729851053	29.976505829925276	21.375259116004127
36-37	21.950321562734118	26.570883154968293	29.87860945796752	21.600185824330065
38-39	21.82715320687964	26.681234464912862	29.92879679340868	21.56281553479882
40-41	21.954256685556224	26.3801113915606	30.029560798947387	21.63607112393579
42-43	21.956870098421174	26.474846459951547	29.562887324714175	22.005396116913108
44-45	21.90065109688149	26.888485538836044	29.455406457977375	21.755456906305096
46-47	22.132995001613097	26.748659176725493	28.895159256123637	22.223186565537773
48-49	22.32803888591125	26.86417653907997	28.673968248100984	22.133816326907795
50-51	22.217139295104136	27.08332902089517	28.35223561230166	22.34729607169904
52-53	22.116926010378872	27.217028710902902	28.258287234410616	22.407758044307602
54-55	22.060790225918876	27.14617903505611	28.24944772248456	22.543583016540456
56-57	22.341119193207177	27.04720367296077	28.118710607667303	22.49296652616476
58-59	21.984375418156503	27.09049420572775	28.400280513245203	22.524849862870543
60-61	22.089603480888336	27.21743176550135	28.276022569352293	22.416942184258023
62-63	22.035244290379126	27.035950299887705	28.211149331730578	22.717656078002594
64-65	21.670499083591046	27.049248923167728	28.29629255062132	22.983959442619906
66-67	21.90199359986326	26.98663210362171	28.294767060956843	22.81660723555818
68-69	21.983648578395684	26.79205004018805	28.354859619789675	22.86944176162659
70-71	21.87200951985531	26.647240744223677	28.30231809896249	23.178431636958518
72-73	21.924777482008786	26.708162056174622	28.31653719318526	23.05052326863134
74-75	21.87928146120828	26.997147787206295	28.08425634724545	23.03931440433998
76-77	21.894447936438514	26.793777222213105	27.952700311943257	23.359074529405127
78-79	21.84861077329911	26.850994966210994	28.01629974738644	23.28409451310345
80-81	21.726063125895458	26.835233044175116	28.073398971805226	23.365304858124205
82-83	21.983922414538757	26.5344329693262	28.278559271624964	23.20308534451008
84-85	22.017500825775674	26.671681941668446	27.889823043000366	23.420994189555515
86-87	21.53101806167388	26.77052693460165	28.104504521554453	23.593950482170015
88-89	21.879320138948234	26.484028256869124	27.94570822703789	23.690943377144748
90-91	21.69377687080221	26.505777730727026	27.94671364119312	23.853731757277647
92-93	21.829083581927133	26.434786414358747	27.94734463971839	23.788785363995732
94-95	21.365339050400134	26.312055741401295	28.11503132681538	24.207573881383183
96-97	21.374651670760542	26.283086934491255	28.066624310974564	24.275637083773635
98-99	21.33453450842678	26.351096501052655	27.925177023253323	24.38919196726724
100	21.45641103338643	26.126096267666714	27.770131568395534	24.647361130551324
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	301.0
1	195.5
2	307.5
3	524.0
4	344.0
5	239.0
6	558.5
7	1406.5
8	2646.5
9	3221.0
10	2419.0
11	1590.0
12	1604.0
13	1816.5
14	2112.0
15	2468.5
16	2856.5
17	3278.5
18	3779.5
19	4455.5
20	5318.5
21	6264.0
22	7569.0
23	9342.0
24	11733.0
25	15109.0
26	19584.0
27	24445.0
28	29459.5
29	35895.5
30	42229.5
31	49066.5
32	57605.0
33	65199.5
34	72927.5
35	81317.5
36	89292.5
37	97782.5
38	102062.5
39	103915.5
40	105971.0
41	106731.5
42	108733.0
43	113534.5
44	118880.5
45	126460.5
46	133564.5
47	138081.5
48	139963.0
49	136592.5
50	131602.5
51	127992.5
52	123579.5
53	117990.5
54	111417.5
55	100614.0
56	89686.5
57	77482.0
58	66101.0
59	57509.0
60	46774.0
61	36186.5
62	28176.5
63	21636.5
64	15377.5
65	10474.5
66	7603.0
67	5493.5
68	3896.5
69	2697.5
70	1983.0
71	1517.5
72	1231.0
73	1110.0
74	897.0
75	654.0
76	462.5
77	292.5
78	203.0
79	135.0
80	95.0
81	67.5
82	50.0
83	37.0
84	22.5
85	13.5
86	9.5
87	5.0
88	4.5
89	2.5
90	0.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0025135353880647287
2	0.0032823815067668806
3	2.0699703195827177E-4
4	0.05157183196217514
5	0.002010828310451783
6	0.004406079680254642
7	2.9571004565467394E-5
8	2.6613904108920656E-4
9	5.914200913093479E-5
10-11	1.7742602739280437E-4
12-13	0.0
14-15	2.9571004565467394E-5
16-17	2.3656803652373915E-4
18-19	1.4785502282733698E-4
20-21	1.9221152967553807E-4
22-23	0.0
24-25	0.0
26-27	2.2178253424100545E-4
28-29	1.4785502282733697E-5
30-31	8.871301369640218E-5
32-33	1.1828401826186958E-4
34-35	2.9571004565467394E-5
36-37	7.392751141366849E-5
38-39	9.31486643812223E-4
40-41	4.87921575330212E-4
42-43	0.0
44-45	3.8442305935107614E-4
46-47	0.0
48-49	1.0349851597913589E-4
50-51	0.0
52-53	4.1399406391654355E-4
54-55	3.6963755706834244E-4
56-57	1.4785502282733698E-4
58-59	9.610576483776903E-4
60-61	7.540606164194186E-4
62-63	2.5135353880647286E-4
64-65	3.8442305935107614E-4
66-67	0.001315909703163299
68-69	0.002631819406326598
70-71	0.0037555175798143587
72-73	0.003267596004484147
74-75	0.0031345264839395437
76-77	0.004332152168840973
78-79	0.0012863386985978316
80-81	0.0010645561643568262
82-83	7.836316209848859E-4
84-85	6.505621004402828E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	8.871301369640218E-5
94-95	1.0349851597913589E-4
96-97	3.6963755706834244E-4
98-99	7.392751141366849E-5
100	0.07049727488407427
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3381691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.25851107849557
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.32511248340151	38.308024961819946
2	15.880908343574124	17.55110699279927
3	6.356950033444043	10.538267815455313
4	3.155810035381802	6.975414552070913
5	1.7437078457919415	4.817734965717683
6	1.032094826721237	3.421921404986007
7	0.6551028185880893	2.5340004450952525
8	0.462724773756722	2.045558562954415
9	0.3155866748117675	1.5694964789680472
>10	1.0452477187004734	8.690513455576495
>50	0.016248709391285157	0.595320474261886
>100	0.008728086964484653	0.8797942341747167
>500	0.0011004496734969356	0.4293130259482431
>1k	4.232498744218456E-4	0.345489868125237
>5k	8.464997488436911E-5	0.2735478945026247
>10k+	1.6929994976873822E-4	1.0244948675438588
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11501	0.3400961235074405	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10401	0.30756801848542636	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5848	0.17293123469885333	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.914200913093479E-5
2	0.0	0.0	0.0	0.0	5.914200913093479E-5
3	0.0	0.0	0.0	0.0	5.914200913093479E-5
4	0.0	0.0	0.0	0.0	5.914200913093479E-5
5	0.0	0.0	0.0	0.0	1.1828401826186958E-4
6	0.0	0.0	0.0	0.0	1.1828401826186958E-4
7	0.0	0.0	0.0	0.0	1.4785502282733698E-4
8	0.0	0.0	0.0	0.0	1.4785502282733698E-4
9	0.0	0.0	0.0	0.0	1.7742602739280437E-4
10-11	0.0	0.0	0.0	0.0	2.3656803652373915E-4
12-13	0.0	0.0	0.0	0.0	2.3656803652373915E-4
14-15	0.0	0.0	0.0	1.4785502282733697E-5	2.5135353880647286E-4
16-17	0.0	0.0	0.0	2.9571004565467394E-5	2.6613904108920656E-4
18-19	0.0	0.0	0.0	4.435650684820109E-5	2.6613904108920656E-4
20-21	0.0	0.0	0.0	1.4785502282733698E-4	3.252810502201413E-4
22-23	0.0	0.0	0.0	3.4006655250287503E-4	3.252810502201413E-4
24-25	0.0	0.0	0.0	0.001020199657508625	3.252810502201413E-4
26-27	0.0	0.0	0.0	0.0019812573058863156	3.252810502201413E-4
28-29	0.0	0.0	0.0	0.006091626940486284	3.252810502201413E-4
30-31	0.0	0.0	0.0	0.016885043606881884	3.252810502201413E-4
32-33	0.0	0.0	0.0	0.03999478367479465	3.252810502201413E-4
34-35	0.0	0.0	0.0	0.07636711929031956	3.252810502201413E-4
36-37	0.0	0.0	0.0	0.13574569645777806	3.252810502201413E-4
38-39	0.0	0.0	0.0	0.22402993058798099	3.5485205478560873E-4
40-41	0.0	0.0	0.0	0.32516276620187945	3.5485205478560873E-4
42-43	0.0	0.0	0.0	0.42638431482947436	3.5485205478560873E-4
44-45	0.0	0.0	0.0	0.5528595013559785	3.6963755706834244E-4
46-47	0.0	0.0	0.0	0.7102955296625268	3.8442305935107614E-4
48-49	0.0	0.0	0.0	0.8852671636763974	3.8442305935107614E-4
50-51	0.0	0.0	0.0	1.0703077247448096	3.9920856163380984E-4
52-53	0.0	0.0	0.0	1.2496558674343694	4.435650684820109E-4
54-55	0.0	0.0	0.0	1.4513596895754224	4.435650684820109E-4
56-57	0.0	0.0	0.0	1.6913283916241904	4.435650684820109E-4
58-59	0.0	0.0	0.0	1.966235235567058	4.435650684820109E-4
60-61	0.0	0.0	0.0	2.257790555080284	4.435650684820109E-4
62-63	0.0	0.0	0.0	2.548118677904043	4.435650684820109E-4
64-65	0.0	0.0	0.0	2.8504082720745334	5.027070776129457E-4
66-67	0.0	0.0	0.0	3.2051272573395972	5.322780821784131E-4
68-69	0.0	0.0	0.0	3.5996340292474978	5.322780821784131E-4
70-71	0.0	0.0	0.0	4.032701391108768	5.322780821784131E-4
72-73	0.0	0.0	0.0	4.467602155253097	5.322780821784131E-4
74-75	0.0	0.0	0.0	4.902976055470473	5.322780821784131E-4
76-77	0.0	0.0	0.0	5.376348696554475	5.322780821784131E-4
78-79	0.0	0.0	0.0	5.907665721084511	5.470635844611468E-4
80-81	0.0	0.0	0.0	6.481210140134033	5.618490867438805E-4
82-83	0.0	0.0	0.0	7.078322649822233	5.618490867438805E-4
84-85	0.0	0.0	0.0	7.665543658483285	5.618490867438805E-4
86-87	0.0	0.0	0.0	8.27485420755474	5.766345890266143E-4
88	0.0	0.0	0.0	8.7550281796888	5.914200913093479E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6860	0.0	58.963	1
GTATCAA	12470	0.0	41.03625	1
ATCAACG	15270	0.0	32.953274	3
CAACGCA	15495	0.0	32.48541	5
TATCAAC	15905	0.0	32.024857	2
TCAACGC	16240	0.0	31.042461	4
AACGCAG	16500	0.0	30.847197	6
ACGCAGA	17980	0.0	28.072807	7
CGCAGAG	18070	0.0	27.776934	8
GCAGAGT	20265	0.0	24.605944	9
TACATGG	9355	0.0	24.081533	2
GTACATG	9780	0.0	24.045645	1
GAGTACT	14170	0.0	23.183817	12-13
ACATGGG	9485	0.0	22.807325	3
TACGGGC	750	0.0	22.57297	4
CAGAGTA	19545	0.0	22.410496	10-11
CTAACGC	525	0.0	22.394146	3
GTACTAG	1430	0.0	22.036572	1
TAACGCC	475	0.0	21.780933	4
AGAGTAC	18480	0.0	21.743795	10-11
>>END_MODULE