FastQCFastQC Report
Mon 16 Jan 2017
ERR1146517_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146517_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229929
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11250.027.165621
GTACATG107250.025.2513141
TACATGG105050.024.434392
ACATGGG106750.023.296613
TATACGA2103.8504368E-822.3864692
GTGATCG7800.022.2910398
TGATCGC8600.020.2168279
GCATCGT12050.019.8886978
CATGGGG71350.019.8321914
TAGGCAT19200.019.0972735
GGCGTCG8150.019.0274038
GTATCAA103200.018.9983181
TACGGGC8450.018.9156114
CGTTAAC3759.094947E-1218.8069691
GGTATCA80850.018.376631
ATACGGG8600.018.039333
CTAGGCA22250.017.7479214
GTATAAG15800.017.5571371
CGCAATA1650.001569539117.0951232
TACTAGG19400.016.9629442
Produced by FastQC (version 0.11.5)